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The map label for this gene is asnB [H]

Identifier: 126173960

GI number: 126173960

Start: 2013017

End: 2014681

Strand: Reverse

Name: asnB [H]

Synonym: Sbal_1731

Alternate gene names: 126173960

Gene position: 2014681-2013017 (Counterclockwise)

Preceding gene: 126173961

Following gene: 126173957

Centisome position: 39.29

GC content: 47.69

Gene sequence:

>1665_bases
ATGTGTTCAATCTTTTCGATTTTAGATATCCAATCTGATGCCAAAGAACTGCGCCAAGTCGCGCTAGAAATGTCTAAGCT
AATGCGCCACCGTGGCCCTGACTGGTCAGGTATTTATGCCAGTGATAAGGCAATTCTTGCCCACGAACGTTTAGCAATTG
TTGACATTGAACACGGTGCTCAGCCACTGCTGACCGAAGATGGCAGTTTGATCCTTGCGGTCAACGGCGAAATCTATAAC
CACAAAGAATTGAAAGCCCAGCTAGGCGATAAGTACAGCTATCAAACCAACTCTGACTGCGAAGTAATCTTGGCGCTGTA
TCAAGAATATGGCACTGACTTCCTTGATAAATTAAACGGTATCTTCGCCTTTGTGTTATATGACAAAACCCAAGACAGAT
ACCTTATCGGCCGCGATCACATGGGTATCATCCCGCTATACACAGGCCGTGACGCTGCGGGTAACTTCTATGTTGCCTCT
GAAATGAAAGCGCTGATGCCAGTGTGTAAAACCGTAGAAGAGTTCTTACCGGGCCAGTATCTGTATTCAAAAGATGGTGA
AGCCGTTAAATACTATAGCCGCGATTGGCAAAGCTATGATGCGGTTAAAGATAACCCAGCCAGCCAAGAAGAATTGCGCG
ACGCATTAGAAGCAGCGGTTAAACGCCAATTGATGTCAGACGTGCCCTATGGTGTGTTGCTATCAGGCGGCTTAGATTCA
TCGATCATTTCCGCCGTGACGCAAACCTTTGCGAAACGCCGCATTGAAGACGATGGCGAAACCAACGCTTGGTGGCCACA
GTTGCATTCTTTTGCCGTCGGCCTTGAAGGTGCACCAGATTTAATCGCCGCCAAAAAAGTCGCAGATGCCATTGGTACGA
TTCACCATGAGATCCATTTCACCTTCCAAGAAGGCTTAGATGCGATTAAAGAAGTGATCTATCACTTAGAAACCTACGAT
GTCACCACCATACGTGCCGCGACACCTATGTATTTAATGGCGCGTAAAATCAAAGCCATGGGCATTAAGATGGTGTTATC
GGGAGAAGGTGCTGATGAGCTATTTGGCGGCTATTTATACTTCCATAAAGCGCCAAACGCACAGGCATTCCACGAGGAAT
TAGTCCGCAAACTCGATAAGCTGCATCTGTTCGATTGTCTGCGTGCCAACAAAGCCATGGCCGCTTGGGGATTAGAAGCT
CGCGTGCCCTTCCTCGATAAAGAGTTTATGGATGTGGCGATGCGCATCAACCCTGAAGCGAAAATGTCTAAGGATGGTCG
TATCGAGAAACACATTCTGCGTAAAGCCTTTGAGCATAAATTGCCAAAAGAAGTGGCGTGGCGTCAAAAGGAACAATTCA
GTGACGGCGTGGGCTACTCTTGGATTGACGGTTTGAAAGAACAAGCTGCAGCGCGGGTGGATGATTTACAATTGGCGAAC
GCGAAATTCCGTTTCCCATACAACACGCCAGAAACCAAAGAAGCCTATTTCTATCGCTGCTTCTTTGAAGAACATTATCC
ATTGAGCGGCGCCGCTGAAACGGTTCCAGGTGGTAAATCCGTCGCCTGTTCAACGCCAGAAGCATTAGCTTGGGACGAAA
GCTTACGCGGCATTATCGACCCATCGGGCCGAGCAGTACGCTCAGTGCACACCTCAAGTTATTAA

Upstream 100 bases:

>100_bases
ATAAAACATCAAAAATAGTGTTTGGCAAATACCTAAATTGCTGGCACATTAAACCTCAATTGAGACATCCTCAAATGACA
GACAGTGTGGGAGTTGTACT

Downstream 100 bases:

>100_bases
TAGCCTAACGTTGAAGGACTAAGTTCATAAATTAGGTTGATGAGCTAGATTGATGAGCTAAGTTAATTAGATTGATGAAC
TGGTTAATACAGCCAACCAC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 554; Mature: 554

Protein sequence:

>554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNGIFAFVLYDKTQDRYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFLPGQYLYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTSSY

Sequences:

>Translated_554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNGIFAFVLYDKTQDRYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFLPGQYLYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTSSY
>Mature_554_residues
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGAQPLLTEDGSLILAVNGEIYN
HKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNGIFAFVLYDKTQDRYLIGRDHMGIIPLYTGRDAAGNFYVAS
EMKALMPVCKTVEEFLPGQYLYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHFTFQEGLDAIKEVIYHLETYD
VTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLYFHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEA
RVPFLDKEFMDVAMRINPEAKMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIIDPSGRAVRSVHTSSY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=543, Percent_Identity=40.8839779005525, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI168229252, Length=543, Percent_Identity=40.8839779005525, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI168229250, Length=543, Percent_Identity=40.8839779005525, Blast_Score=348, Evalue=5e-96,
Organism=Homo sapiens, GI296010848, Length=514, Percent_Identity=42.4124513618677, Blast_Score=348, Evalue=6e-96,
Organism=Homo sapiens, GI296010852, Length=449, Percent_Identity=43.4298440979955, Blast_Score=317, Evalue=2e-86,
Organism=Homo sapiens, GI296010850, Length=449, Percent_Identity=43.4298440979955, Blast_Score=317, Evalue=2e-86,
Organism=Escherichia coli, GI1786889, Length=554, Percent_Identity=70.3971119133574, Blast_Score=830, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=569, Percent_Identity=42.530755711775, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI71993933, Length=531, Percent_Identity=43.6911487758945, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17560178, Length=523, Percent_Identity=35.5640535372849, Blast_Score=295, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI25147560, Length=312, Percent_Identity=39.1025641025641, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321563, Length=545, Percent_Identity=50.6422018348624, Blast_Score=542, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6325403, Length=546, Percent_Identity=51.2820512820513, Blast_Score=538, Evalue=1e-154,
Organism=Drosophila melanogaster, GI45553209, Length=578, Percent_Identity=47.2318339100346, Blast_Score=501, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62399; Mature: 62399

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCC
IFAFVLYDKTQDRYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVEEFLPGQY
EEEEEEEECCCCCEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
LYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHTSSY
CCCHHHHHHHCCCC
>Mature Secondary Structure
MCSIFSILDIQSDAKELRQVALEMSKLMRHRGPDWSGIYASDKAILAHERLAIVDIEHGA
CCCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCEEEEEECCCC
QPLLTEDGSLILAVNGEIYNHKELKAQLGDKYSYQTNSDCEVILALYQEYGTDFLDKLNG
CCCEECCCCEEEEECCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHCC
IFAFVLYDKTQDRYLIGRDHMGIIPLYTGRDAAGNFYVASEMKALMPVCKTVEEFLPGQY
EEEEEEEECCCCCEEEECCCCCEEEEECCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCE
LYSKDGEAVKYYSRDWQSYDAVKDNPASQEELRDALEAAVKRQLMSDVPYGVLLSGGLDS
EECCCCCEEHHHHHCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCH
SIISAVTQTFAKRRIEDDGETNAWWPQLHSFAVGLEGAPDLIAAKKVADAIGTIHHEIHF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHEEE
TFQEGLDAIKEVIYHLETYDVTTIRAATPMYLMARKIKAMGIKMVLSGEGADELFGGYLY
EHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEEEECCCHHHHHCCEEE
FHKAPNAQAFHEELVRKLDKLHLFDCLRANKAMAAWGLEARVPFLDKEFMDVAMRINPEA
EEECCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHCCCCH
KMSKDGRIEKHILRKAFEHKLPKEVAWRQKEQFSDGVGYSWIDGLKEQAAARVDDLQLAN
HCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEECC
AKFRFPYNTPETKEAYFYRCFFEEHYPLSGAAETVPGGKSVACSTPEALAWDESLRGIID
CEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHCCCCC
PSGRAVRSVHTSSY
CCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]