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The map label for this gene is vat [H]

Identifier: 126173803

GI number: 126173803

Start: 1827270

End: 1827926

Strand: Reverse

Name: vat [H]

Synonym: Sbal_1569

Alternate gene names: 126173803

Gene position: 1827926-1827270 (Counterclockwise)

Preceding gene: 126173804

Following gene: 126173802

Centisome position: 35.65

GC content: 48.1

Gene sequence:

>657_bases
ATGCCTGACACAACAACCCACGCCCAATTAGGCCCAAATCCCAACGACAAAGCGCCGATGAAAGGCTTCCCTCAGGTTGG
ATTTCTTAAAAACTTTATTACCCGCGAGAACATAATCGTCGGCGATTATACCTATTATGACGATCCTGCGGGGCCTGAAA
GATTCGAGTCGAATGTGCTTTATCACTTTGACTTTATTGGCGATAAACTGATTATCGGTAAATTCTGCGCCATTGCTAAA
GACGTGAAATTCATTATGAATGGCGCCAATCATCAAGTGTCTGGCTTCTCGACTTATCCCTTTTATATCTTCGGCAATGG
CTGGGAAAAGGTAATGCCAGATCCCACAGATTTGCCCCATAAAGGCGACACCTGTATCGGTAACGATGTGTGGATTGGTT
ATAACGCCACTATCATGCCTGGAGTGAAGATTGGCCACGGCGCAATTGTTGCCAGTCAATCCGTGGTGACAAAAGATGTG
CCGCCCTACGCTGTGGTTGGCGGTAATCCTGCCACTGTCATCAAGCTCAGGTTCGAGCAAGATGTGATAGATAAGCTCGT
TGCCATCGCTTGGTGGGATTGGCCGATAGAAAAAATCACTCAGCATCTACACGCCATCGCGGGCGCGGATCTGACTCCAT
TACAGCAGGCGACCTAA

Upstream 100 bases:

>100_bases
CAGGCACCACAATCAACATCGCAATAGGCACCACAAATTCGCACATCAATCGCATAAAAGAATGCACGAGTTAAGACGTA
CTTTAACGATGGAGACAGTT

Downstream 100 bases:

>100_bases
GCAAAGCCAGTCGCGACCAAGACTGGGTCCCCAAATATCAACTGGCTAATATCTCGAGCGACAGATATATCAACTGGCTA
ATATCAACTGACCAATATTA

Product: hexapaptide repeat-containing transferase

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MPDTTTHAQLGPNPNDKAPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIAK
DVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPHKGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTKDV
PPYAVVGGNPATVIKLRFEQDVIDKLVAIAWWDWPIEKITQHLHAIAGADLTPLQQAT

Sequences:

>Translated_218_residues
MPDTTTHAQLGPNPNDKAPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIAK
DVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPHKGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTKDV
PPYAVVGGNPATVIKLRFEQDVIDKLVAIAWWDWPIEKITQHLHAIAGADLTPLQQAT
>Mature_217_residues
PDTTTHAQLGPNPNDKAPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVLYHFDFIGDKLIIGKFCAIAKD
VKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPHKGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTKDVP
PYAVVGGNPATVIKLRFEQDVIDKLVAIAWWDWPIEKITQHLHAIAGADLTPLQQAT

Specific function: Inactivates the A compounds of virginiamycin-like antibiotics, thus providing resistance to these antibiotics [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786664, Length=50, Percent_Identity=66, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 24049; Mature: 23917

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTTTHAQLGPNPNDKAPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCEECCCCCCHHHHHCCEE
YHFDFIGDKLIIGKFCAIAKDVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPH
EEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCHHCCCCCCCCCC
KGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTKDVPPYAVVGGNPATVIKLRFEQ
CCCEECCCCEEECCCCEECCCCEECCCEEEECCCEEECCCCCEEEECCCCEEEEEEECCH
DVIDKLVAIAWWDWPIEKITQHLHAIAGADLTPLQQAT
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
PDTTTHAQLGPNPNDKAPMKGFPQVGFLKNFITRENIIVGDYTYYDDPAGPERFESNVL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEEEECCEECCCCCCHHHHHCCEE
YHFDFIGDKLIIGKFCAIAKDVKFIMNGANHQVSGFSTYPFYIFGNGWEKVMPDPTDLPH
EEEECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEECCCHHCCCCCCCCCC
KGDTCIGNDVWIGYNATIMPGVKIGHGAIVASQSVVTKDVPPYAVVGGNPATVIKLRFEQ
CCCEECCCCEEECCCCEECCCCEECCCEEEECCCEEECCCCCEEEECCCCEEEEEEECCH
DVIDKLVAIAWWDWPIEKITQHLHAIAGADLTPLQQAT
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 90 {maltose}} 62 {glucose}} 0.018 {acetyl-CoA}} 40 {triethanolamine}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8344533; 3149758 [H]