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The map label for this gene is queF [H]

Identifier: 126173669

GI number: 126173669

Start: 1663391

End: 1664281

Strand: Reverse

Name: queF [H]

Synonym: Sbal_1431

Alternate gene names: 126173669

Gene position: 1664281-1663391 (Counterclockwise)

Preceding gene: 126173671

Following gene: 126173667

Centisome position: 32.46

GC content: 45.57

Gene sequence:

>891_bases
ATGTGCGCCTATTTTGACATGGAAACAGTATTGATGACACAGAATCACGATCCCTATAGTGATGCGCAAGCGCTTGTTGG
CCTGACTTTAGGTAAAGCCACAGACTATCAAGCCGAGTATGATGCGTCGCTGCTGCAAGGGGTTCCACGCTCACTAAACC
GTAATGCTATTGCACTCACTGGGACGTTACCGTTTCATGGTGCCGATATCTGGACGGGTTATGAGCTGTCTTGGTTAAAT
GCTAAAGGTAAGCCTATGGTGGCCATAGCTGAATTTCATTTAAGCTATGAGAGCCTGAACCTGATTGAGTCGAAATCGTT
CAAACTGTATCTCAATAGCTTTAATCAAACCAAGTTCGATAGCATAGATTCACTGCAAAAGACCTTAGTCGCAGACTTAA
GTCAATGTGCTCAAGGTGATATCAGCGTTAAAATCATCGAACCAAAAAGCTTTGGCATTCAGCGTATTATCGAGTTACCA
GGCACGTGTATTGACGATCTCGATATTGAAGTCAGCGATTATAGTTTTAATCCTGATTACTTGGAAAACAGCACGGACGA
TAAGCAAACGGTCGCGGAAACGCTTAATTCAAACCTGCTGAAATCCAACTGTTTGATCACTTCGCAACCCGACTGGGGCA
GCGTAATGATCCGTTATCAAGGGCCTAAAATCGATCGCGAGAAACTGCTGCGCTATTTGATTTCTTTCCGCCAACATAAC
GAGTTCCACGAGCAATGTGTCGAGCGGATTTTTGTCGATTTGAAGCGTTTCTGTCATTGCACTAAATTGACCGTTTACGC
CCGCTATACGCGCCGCGGCGGTTTAGATATCAACCCGTATCGCAGTGACTTCGAGCAACCTGGCGAAAGCCACAGACTCG
CAAGGCAGTAA

Upstream 100 bases:

>100_bases
AGTTCACCTAAACTCGTTAGGTAAGCTTGATGGTAGTTTTGCAAAAATTTATCGAGTGCGGGCAGACAAGACACTTAAGC
ACAATCCAATTTCAGGTATA

Downstream 100 bases:

>100_bases
TTAACGTTCGTTCGAGTATCAGATTACGAACTAAGGTTTAGAACTGAGAAAAAACCAAGGTTACATACCGAAACTAAGCT
CTGAATCGCTTGCCTAGCTG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MCAYFDMETVLMTQNHDPYSDAQALVGLTLGKATDYQAEYDASLLQGVPRSLNRNAIALTGTLPFHGADIWTGYELSWLN
AKGKPMVAIAEFHLSYESLNLIESKSFKLYLNSFNQTKFDSIDSLQKTLVADLSQCAQGDISVKIIEPKSFGIQRIIELP
GTCIDDLDIEVSDYSFNPDYLENSTDDKQTVAETLNSNLLKSNCLITSQPDWGSVMIRYQGPKIDREKLLRYLISFRQHN
EFHEQCVERIFVDLKRFCHCTKLTVYARYTRRGGLDINPYRSDFEQPGESHRLARQ

Sequences:

>Translated_296_residues
MCAYFDMETVLMTQNHDPYSDAQALVGLTLGKATDYQAEYDASLLQGVPRSLNRNAIALTGTLPFHGADIWTGYELSWLN
AKGKPMVAIAEFHLSYESLNLIESKSFKLYLNSFNQTKFDSIDSLQKTLVADLSQCAQGDISVKIIEPKSFGIQRIIELP
GTCIDDLDIEVSDYSFNPDYLENSTDDKQTVAETLNSNLLKSNCLITSQPDWGSVMIRYQGPKIDREKLLRYLISFRQHN
EFHEQCVERIFVDLKRFCHCTKLTVYARYTRRGGLDINPYRSDFEQPGESHRLARQ
>Mature_296_residues
MCAYFDMETVLMTQNHDPYSDAQALVGLTLGKATDYQAEYDASLLQGVPRSLNRNAIALTGTLPFHGADIWTGYELSWLN
AKGKPMVAIAEFHLSYESLNLIESKSFKLYLNSFNQTKFDSIDSLQKTLVADLSQCAQGDISVKIIEPKSFGIQRIIELP
GTCIDDLDIEVSDYSFNPDYLENSTDDKQTVAETLNSNLLKSNCLITSQPDWGSVMIRYQGPKIDREKLLRYLISFRQHN
EFHEQCVERIFVDLKRFCHCTKLTVYARYTRRGGLDINPYRSDFEQPGESHRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=279, Percent_Identity=64.8745519713262, Blast_Score=365, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 33767; Mature: 33767

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCAYFDMETVLMTQNHDPYSDAQALVGLTLGKATDYQAEYDASLLQGVPRSLNRNAIALT
CCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHCCCCEEEEE
GTLPFHGADIWTGYELSWLNAKGKPMVAIAEFHLSYESLNLIESKSFKLYLNSFNQTKFD
ECCCCCCCCEECCEEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCH
SIDSLQKTLVADLSQCAQGDISVKIIEPKSFGIQRIIELPGTCIDDLDIEVSDYSFNPDY
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCC
LENSTDDKQTVAETLNSNLLKSNCLITSQPDWGSVMIRYQGPKIDREKLLRYLISFRQHN
CCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
EFHEQCVERIFVDLKRFCHCTKLTVYARYTRRGGLDINPYRSDFEQPGESHRLARQ
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHCCCCCCCCCHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MCAYFDMETVLMTQNHDPYSDAQALVGLTLGKATDYQAEYDASLLQGVPRSLNRNAIALT
CCEEEEEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHCCCCEEEEE
GTLPFHGADIWTGYELSWLNAKGKPMVAIAEFHLSYESLNLIESKSFKLYLNSFNQTKFD
ECCCCCCCCEECCEEEEEECCCCCCEEEEEEEHHHHHHHHHHHCCCEEEEEECCCCCCCH
SIDSLQKTLVADLSQCAQGDISVKIIEPKSFGIQRIIELPGTCIDDLDIEVSDYSFNPDY
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCC
LENSTDDKQTVAETLNSNLLKSNCLITSQPDWGSVMIRYQGPKIDREKLLRYLISFRQHN
CCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCC
EFHEQCVERIFVDLKRFCHCTKLTVYARYTRRGGLDINPYRSDFEQPGESHRLARQ
HHHHHHHHHHHHHHHHHHHHHHHEEEEEEHHCCCCCCCCCHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA