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The map label for this gene is insF7 [C]

Identifier: 126173619

GI number: 126173619

Start: 1586468

End: 1587235

Strand: Reverse

Name: insF7 [C]

Synonym: Sbal_1381

Alternate gene names: 126173619

Gene position: 1587235-1586468 (Counterclockwise)

Preceding gene: 126173620

Following gene: 126173614

Centisome position: 30.96

GC content: 46.88

Gene sequence:

>768_bases
ATGGCGAGCCGAAGCGCTGAGCTATCGGTCATCAAAGACTGGGTCCAATATTGGCGTAACTATATGCCACGATTGGGGAC
GCGTAAGCTCTATACTTTGATACAGCCTAAGCTGGTTGAACATGATATCAAACTCGGGCGAGACGGGTTCTTTACCTACT
TGAGAAGTGAAGGCTTGCTAGTTAAGCCCAAGAGAAGCTTCACAAAAACGACATTCAGCAAGCATTGGATGAAGAAGCAT
CCCAACCTGCTGAAGGAAGAGGGACTACACGATGTCGAGCATGTACTGGTCAGCGATATTACCTATCTCGAATCAGACCA
AGGCGTGCACTATTTGTCACTGGTGACCGATGCCGCGTCACGTAAGATAGTCGGTCATCACTTGAGTTCGGACATGAAAG
CAGAAAGCGTAGTGAAAGCACTGAAAATGGCGGTCAAAGATAAGCGCTATAGCGCTAATGCGGTCCATCACTCAGACCGA
GGTTCGCAATACTGCTCAGCTGTTTATCAAGATAAACTGCAGGCCAACCATATTCAGCCGTCGATGACAGACGGTTATGA
TTGCTATCAAAATACGTTAGCAGAACGAGTAAACGGGATCCTGAAGCAGGAGTTTTTACTCTACCGATGTAAAACGCTGG
AAGAGCTGAAGAGACTTGTTAGAGAATCGATAGCGATATACAACGAAATGAGACCGCATCTGAGTTTGGATATGGAAACA
CCCAATCAGGTGCATAATAGAAAAGGCCAGCTACTGGAGCTGGCCTAG

Upstream 100 bases:

>100_bases
GTATCTCTGGCAAGCCAAAACCCAAAGCAAAATAAACCTCGCCGTTGCGTGTCGCGCTGTCGGTATTTCACGGCAAGGTA
TTTATCAGGCTGTCGCCCGA

Downstream 100 bases:

>100_bases
AAACTGTCAACCTATCTTAGGACGGGACAATCTACCAGACAGAATGACCGAACATCATCGCTCAGATAAAACCCCAGAAA
CAAAAAAGGACCTCCGAGGA

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MASRSAELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLLVKPKRSFTKTTFSKHWMKKH
PNLLKEEGLHDVEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAVHHSDR
GSQYCSAVYQDKLQANHIQPSMTDGYDCYQNTLAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMET
PNQVHNRKGQLLELA

Sequences:

>Translated_255_residues
MASRSAELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLLVKPKRSFTKTTFSKHWMKKH
PNLLKEEGLHDVEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAVHHSDR
GSQYCSAVYQDKLQANHIQPSMTDGYDCYQNTLAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMET
PNQVHNRKGQLLELA
>Mature_254_residues
ASRSAELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLLVKPKRSFTKTTFSKHWMKKHP
NLLKEEGLHDVEHVLVSDITYLESDQGVHYLSLVTDAASRKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAVHHSDRG
SQYCSAVYQDKLQANHIQPSMTDGYDCYQNTLAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMETP
NQVHNRKGQLLELA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=192, Percent_Identity=31.7708333333333, Blast_Score=95, Evalue=7e-20,
Organism=Escherichia coli, GI1788405, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1787263, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1786753, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1786570, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1786490, Length=202, Percent_Identity=31.6831683168317, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1789981, Length=152, Percent_Identity=32.8947368421053, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29576; Mature: 29445

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CHHHHCCCCCEEECC
>Mature Secondary Structure 
ASRSAELSVIKDWVQYWRNYMPRLGTRKLYTLIQPKLVEHDIKLGRDGFFTYLRSEGLL
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCE
VKPKRSFTKTTFSKHWMKKHPNLLKEEGLHDVEHVLVSDITYLESDQGVHYLSLVTDAAS
ECCCHHHHHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
RKIVGHHLSSDMKAESVVKALKMAVKDKRYSANAVHHSDRGSQYCSAVYQDKLQANHIQP
HHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCC
SMTDGYDCYQNTLAERVNGILKQEFLLYRCKTLEELKRLVRESIAIYNEMRPHLSLDMET
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
PNQVHNRKGQLLELA
CHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]