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The map label for this gene is leuB [H]

Identifier: 126173606

GI number: 126173606

Start: 1567754

End: 1568761

Strand: Reverse

Name: leuB [H]

Synonym: Sbal_1368

Alternate gene names: 126173606

Gene position: 1568761-1567754 (Counterclockwise)

Preceding gene: 126173608

Following gene: 126173601

Centisome position: 30.6

GC content: 47.92

Gene sequence:

>1008_bases
ATGTCAAAAAGAACGATAACCGTAATTCCAGGTGATGGAATTGGCCCAAGTATTATTGATTCAGCCTTAAAAATTCTCGA
TAAAGCGGGCTGTGATTTTGAATATGAATTTGCCGATGCCGGCCTGACTGCACTCGAAAAGCAAGGCGAGTTACTGCCAC
CGCGCACCTTAGAATTAATCGAAAAAAACCGTATCACACTGAAAGGCCCACTGACGACGCCAGTAGGTGAAGGCTTTACC
TCTATCAACGTGACCTTACGTAAAAAATTCGGCCTGTACGCTAACGTACGCCCAGTGATTTCGTTCAAAGGCACTCAAGC
TCGTTACGAAAATATCGATATCATCACAGTACGTGAAAACACTGAAGGCATGTATTCTGGCTATGGCCAAAAAGTGTCTG
AAGATGGTTTGACGGCAGAAGCGACCAGTATCGTGACTCGCCAAGGTGCCGAGCAAATCGCCACTTTCGCCTACGAACTG
GCTCGCAAAGAAAACCGTAAAAAAGTCACTATCGTGCACAAAGCCAACATCATGAAGTCAACGTCAGGCCTGTTCCTGAA
AGTGGCCCGTGAAGTGAGCCTACGTTACCCAGACATCAAGACAGAAGAAATGATCGTCGATGCAACGTGCATGAAGTTAG
TGATGAACCCAGAAAACTTCGATGTGATCGTGACGACCAACCTGTTCGGTGACATTTTATCGGATCTATGTGCCGGTTTA
GTCGGTGGTTTAGGTATGGCACCAGGCGCAAACATTGGCCGTGATGCCGCGATTTTCGAAGCCGTTCACGGTAGTGCACC
GGATATCGCTGGTAAAAACCTCGCGAACCCAACCTCAGTAATTTTAGCCTCTATCCAGATGCTGGAATATTTAGGTATGG
CTGACAAAGCTGAGCAAATCCGTAAAGCGGTATCTGCAGTGATCGAAGAAGGCGACCGTACCACTCGCGATTTAGGCGGC
ACCCACGGCACGACTGACTTCACTCAAGCGGTTCTTGAGCGTCTGTGA

Upstream 100 bases:

>100_bases
AAAATAATAGGCAGAAAAAATACCAAATTCATAGTCCTGCTATCCAGTTAACGCTGGGGCAGACAATTGCAGTAACGACG
AACCGTGGAAGGACTTCTCC

Downstream 100 bases:

>100_bases
TCGCAATGCTGAGCGCGTTTAAGACGAGTCTATAACGCGCTTGTTCAAGCTTAAGCTTCATAAGAAGTAAGCGCTTGAAA
CTCAAATGAGTAAACATGAA

Product: isocitrate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPPRTLELIEKNRITLKGPLTTPVGEGFT
SINVTLRKKFGLYANVRPVISFKGTQARYENIDIITVRENTEGMYSGYGQKVSEDGLTAEATSIVTRQGAEQIATFAYEL
ARKENRKKVTIVHKANIMKSTSGLFLKVAREVSLRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKAEQIRKAVSAVIEEGDRTTRDLGG
THGTTDFTQAVLERL

Sequences:

>Translated_335_residues
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPPRTLELIEKNRITLKGPLTTPVGEGFT
SINVTLRKKFGLYANVRPVISFKGTQARYENIDIITVRENTEGMYSGYGQKVSEDGLTAEATSIVTRQGAEQIATFAYEL
ARKENRKKVTIVHKANIMKSTSGLFLKVAREVSLRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKAEQIRKAVSAVIEEGDRTTRDLGG
THGTTDFTQAVLERL
>Mature_334_residues
SKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPPRTLELIEKNRITLKGPLTTPVGEGFTS
INVTLRKKFGLYANVRPVISFKGTQARYENIDIITVRENTEGMYSGYGQKVSEDGLTAEATSIVTRQGAEQIATFAYELA
RKENRKKVTIVHKANIMKSTSGLFLKVAREVSLRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGLV
GGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKAEQIRKAVSAVIEEGDRTTRDLGGT
HGTTDFTQAVLERL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=335, Percent_Identity=47.1641791044776, Blast_Score=324, Evalue=6e-89,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=41.9642857142857, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI28178838, Length=310, Percent_Identity=43.2258064516129, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI28178821, Length=338, Percent_Identity=40.2366863905325, Blast_Score=231, Evalue=8e-61,
Organism=Homo sapiens, GI28178816, Length=338, Percent_Identity=39.9408284023669, Blast_Score=228, Evalue=9e-60,
Organism=Homo sapiens, GI28178819, Length=198, Percent_Identity=47.4747474747475, Blast_Score=171, Evalue=8e-43,
Organism=Escherichia coli, GI87081683, Length=338, Percent_Identity=38.1656804733728, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788101, Length=358, Percent_Identity=34.3575418994413, Blast_Score=192, Evalue=2e-50,
Organism=Escherichia coli, GI1787381, Length=361, Percent_Identity=34.0720221606648, Blast_Score=189, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI71986051, Length=332, Percent_Identity=46.3855421686747, Blast_Score=323, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI25144293, Length=338, Percent_Identity=40.2366863905325, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=40.1759530791789, Blast_Score=236, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI17505779, Length=335, Percent_Identity=39.7014925373134, Blast_Score=230, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6324709, Length=343, Percent_Identity=45.1895043731778, Blast_Score=293, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=44.047619047619, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=350, Percent_Identity=40.2857142857143, Blast_Score=227, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319830, Length=361, Percent_Identity=29.6398891966759, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=44.2771084337349, Blast_Score=317, Evalue=8e-87,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=44.2771084337349, Blast_Score=317, Evalue=8e-87,
Organism=Drosophila melanogaster, GI24661184, Length=339, Percent_Identity=42.7728613569322, Blast_Score=291, Evalue=4e-79,
Organism=Drosophila melanogaster, GI161078633, Length=336, Percent_Identity=39.2857142857143, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24650122, Length=336, Percent_Identity=39.2857142857143, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI161078637, Length=336, Percent_Identity=39.2857142857143, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI161078635, Length=336, Percent_Identity=39.2857142857143, Blast_Score=233, Evalue=1e-61,
Organism=Drosophila melanogaster, GI161078639, Length=331, Percent_Identity=39.5770392749245, Blast_Score=229, Evalue=2e-60,
Organism=Drosophila melanogaster, GI281362242, Length=339, Percent_Identity=41.2979351032448, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24648872, Length=339, Percent_Identity=41.2979351032448, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI20130355, Length=331, Percent_Identity=29.0030211480363, Blast_Score=171, Evalue=7e-43,
Organism=Drosophila melanogaster, GI45550436, Length=223, Percent_Identity=24.6636771300448, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 36206; Mature: 36075

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPPRTLELI
CCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHCCCCCCCHHHHHH
EKNRITLKGPLTTPVGEGFTSINVTLRKKFGLYANVRPVISFKGTQARYENIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEEEEEECCCCEECCCEEEEECCCCCHHCCEEEEEEECC
TEGMYSGYGQKVSEDGLTAEATSIVTRQGAEQIATFAYELARKENRKKVTIVHKANIMKS
CCCHHHCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSLRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKAEQI
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
RKAVSAVIEEGDRTTRDLGGTHGTTDFTQAVLERL
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure 
SKRTITVIPGDGIGPSIIDSALKILDKAGCDFEYEFADAGLTALEKQGELLPPRTLELI
CCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEHHHCCHHHHHHCCCCCCCHHHHHH
EKNRITLKGPLTTPVGEGFTSINVTLRKKFGLYANVRPVISFKGTQARYENIDIITVREN
HCCCEEEECCCCCCCCCCCEEEEEEEEECCCCEECCCEEEEECCCCCHHCCEEEEEEECC
TEGMYSGYGQKVSEDGLTAEATSIVTRQGAEQIATFAYELARKENRKKVTIVHKANIMKS
CCCHHHCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCCEEEEEEECCHHHC
TSGLFLKVAREVSLRYPDIKTEEMIVDATCMKLVMNPENFDVIVTTNLFGDILSDLCAGL
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHH
VGGLGMAPGANIGRDAAIFEAVHGSAPDIAGKNLANPTSVILASIQMLEYLGMADKAEQI
HCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHH
RKAVSAVIEEGDRTTRDLGGTHGTTDFTQAVLERL
HHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]