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The map label for this gene is gyrB [H]

Identifier: 125624430

GI number: 125624430

Start: 1606663

End: 1608618

Strand: Reverse

Name: gyrB [H]

Synonym: llmg_1631

Alternate gene names: 125624430

Gene position: 1608618-1606663 (Counterclockwise)

Preceding gene: 125624431

Following gene: 125624429

Centisome position: 63.59

GC content: 39.01

Gene sequence:

>1956_bases
TTGAACGAAGAAAATAAAAATTTAGAAATGCTTGCAGACGAATATGATGCCAGCCAGATTCAGGTCCTTGAAGGTCTTGA
AGCTGTACGTATGCGCCCTGGGATGTATATCGGTTCAACATCAAAAGAAGGTTTGCACCATTTGGTCTGGGAGATTGTTG
ACAATTCAATTGACGAAGCACTTGCTGGATTTGCAAGCCATATTGAGGTTTTTATTGAACCTGATAATTCAATTACAGTT
GTTGATGATGGACGGGGAATTCCTGTTGATATTCAGGAAAAAACAGGTCGTCCAGCGGTAGAAACCGTCTTTACCGTTCT
ACATGCCGGAGGTAAATTTGGCGGAGGCGGTTATAAGGTTTCTGGTGGATTACACGGTGTAGGTTCTTCTGTTGTTAACG
CCTTGTCAACGACTTTAGATGTTACCGTCCATAAAGATGGTCAAAAATATTTTCAAGAGTATCACCGGGGTCTCGTTAGA
GATGATTTGAAAATTATTGGTGAAACTGATCTTAGAGGAACAACTGTTCACTTTGTACCAGACCCAGAAATTTTTACAGA
AACTGTTGAATTTGACTTTGACAAACTCGCAACGCGTGTTCGTGAACTTGCCTTCTTGAATCGTGGTCTTCGTATTTCCT
TAACAGATAAACGTGAAGGAAATGAAAAAAATGAAGTTCACTTCCATTATGAAGGTGGGATTCAGTCTTATGTTGAATAT
TTGAATGAAAATAAGACAGTTATTTTTGAACCAGCTATTTTTACCGAAGGTGAAATGGATGGAATTGCTGTTGAAGTTGC
CATGCAATATACAGATACTTATCATTCAGTAGTGATGTCTTTTGCGAATAATATTAATACTCATGAAGGTGGAACACATG
AACAAGGGTTTAGAACTGCCCTAACTCGTGTCATTAACAACTACGCCAAACAAAATAAAATTCTTAAAGAAAATGAAGAC
AATTTGACTGGTGACGATGTGCGTGAAGGATTGACTGCCGTTATCTCAGTTAAACACCCTAATCCACAGTTTGAAGGACA
AACCAAAACAAAACTTGGAAACTCAGAAGTAACAGGAATTGTCAACAAACTTTTTGCCGAAGCACTGACAACATTTATGT
TGGAAAATCCACAAGTTGCTCGTAAAATTGTAGAAAAAGGGATTTTAGCAAGTAAAGCCAGAATTGCAGCTAAACGTGCG
CGTGAAGTGACGCGTAAAAAATCAGGTCTTGAAATTTCAAATCTTCCTGGTAAATTAGCAGACTGTTCATCGAATAATCC
TGAACAAACAGAATTATTTATTGTCGAAGGAGATTCAGCCGGAGGGTCTGCTAAATCGGGACGTAATCGTGAATTCCAAG
CCATTCTCCCAATTCGTGGTAAGATTTTGAACGTTGAAAAAGCAACAATGGATAAAATTTTAGCCAATGAAGAAATCCGT
TCATTATTTACTGCCATGGGAACAGGTTTTGGGGGAGATTATGATTTGTCCAAAGCACGTTATCATAAACTCGTTATCAT
GACCGATGCCGACGTTGATGGGGCTCATATCCGAACACTACTACTCACACTATTCTATCGTTACATGCGTCCAGTTGTAG
AAGCAGGCTACGTTTATATTGCTCAACCACCAATTTATGGTATAAAAGTAGGTTCTGAAACTAAAGAATACATTCAACCA
GGTGAAAATCAAGAAAAAGATTTACAAGAAGCTTTAGCACGCTGGGCACAAGGGAGAACAAAACCTACGGTCCAACGTTA
TAAAGGTCTTGGTGAAATGGATGACCATCAGCTATGGGAAACAACAATGGATCCTGAACATCGTTTGATGGCACGCGTTT
CTGTCGAAGATGCAGCAGAAGCAGATAAAATATTTGATATGCTCATGGGAGACCGTGTTGAACCTCGCCGTGAATTTATT
GAAAATAATGCTCAATACTCAACGATTGATGCTTAA

Upstream 100 bases:

>100_bases
ATATCTGAAGAGTTAGAGATGAAAATACCTTAGAAAAGTGCTAAAATCGCCGATTTGTGGTATAATGGTAATAAGTTTTT
CATAGAAATGAGAGTTAACT

Downstream 100 bases:

>100_bases
TAAAAAAATCCAGTTGTATCTGGATTTTTTTATTAAGCATAATGTTAGCGCTTTAAATTGGTCTGTAATATAATGATTTT
AAGAAGGAGGAATGCCTATG

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 651; Mature: 651

Protein sequence:

>651_residues
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIEVFIEPDNSITV
VDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTTLDVTVHKDGQKYFQEYHRGLVR
DDLKIIGETDLRGTTVHFVPDPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTALTRVINNYAKQNKILKENED
NLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGIVNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRA
REVTRKKSGLEISNLPGKLADCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
SLFTAMGTGFGGDYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSETKEYIQP
GENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWETTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFI
ENNAQYSTIDA

Sequences:

>Translated_651_residues
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIEVFIEPDNSITV
VDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTTLDVTVHKDGQKYFQEYHRGLVR
DDLKIIGETDLRGTTVHFVPDPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTALTRVINNYAKQNKILKENED
NLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGIVNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRA
REVTRKKSGLEISNLPGKLADCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
SLFTAMGTGFGGDYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSETKEYIQP
GENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWETTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFI
ENNAQYSTIDA
>Mature_651_residues
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEALAGFASHIEVFIEPDNSITV
VDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTTLDVTVHKDGQKYFQEYHRGLVR
DDLKIIGETDLRGTTVHFVPDPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTALTRVINNYAKQNKILKENED
NLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGIVNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRA
REVTRKKSGLEISNLPGKLADCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
SLFTAMGTGFGGDYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYIAQPPIYGIKVGSETKEYIQP
GENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWETTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFI
ENNAQYSTIDA

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=688, Percent_Identity=26.0174418604651, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI19913406, Length=680, Percent_Identity=26.1764705882353, Blast_Score=125, Evalue=1e-28,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=53.9426523297491, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1789408, Length=645, Percent_Identity=40.4651162790698, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI17535065, Length=677, Percent_Identity=25.1107828655835, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI212645845, Length=604, Percent_Identity=24.5033112582781, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI212645657, Length=229, Percent_Identity=25.764192139738, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=646, Percent_Identity=26.0061919504644, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI17136538, Length=690, Percent_Identity=25.9420289855072, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 72593; Mature: 72593

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEA
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LAGFASHIEVFIEPDNSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKV
HHHHHHCEEEEEECCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTTLDVTVHKDGQKYFQEYHRGLVRDDLKIIGETDLRGTTVHFVP
CCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECC
DPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHH
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTA
HCCCCEEEECCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LTRVINNYAKQNKILKENEDNLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHH
VNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
DCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHH
SLFTAMGTGFGGDYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYI
HHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPIYGIKVGSETKEYIQPGENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWE
ECCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHH
TTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFIENNAQYSTIDA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MNEENKNLEMLADEYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLHHLVWEIVDNSIDEA
CCCCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHH
LAGFASHIEVFIEPDNSITVVDDGRGIPVDIQEKTGRPAVETVFTVLHAGGKFGGGGYKV
HHHHHHCEEEEEECCCCEEEEECCCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGSSVVNALSTTLDVTVHKDGQKYFQEYHRGLVRDDLKIIGETDLRGTTVHFVP
CCCCCHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECC
DPEIFTETVEFDFDKLATRVRELAFLNRGLRISLTDKREGNEKNEVHFHYEGGIQSYVEY
CCHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEEECCCHHHHHHH
LNENKTVIFEPAIFTEGEMDGIAVEVAMQYTDTYHSVVMSFANNINTHEGGTHEQGFRTA
HCCCCEEEECCCEECCCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
LTRVINNYAKQNKILKENEDNLTGDDVREGLTAVISVKHPNPQFEGQTKTKLGNSEVTGI
HHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEECCCCCCCCCHHHCCCCHHHHHH
VNKLFAEALTTFMLENPQVARKIVEKGILASKARIAAKRAREVTRKKSGLEISNLPGKLA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
DCSSNNPEQTELFIVEGDSAGGSAKSGRNREFQAILPIRGKILNVEKATMDKILANEEIR
CCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEECHHHHHHHHHHHHHHH
SLFTAMGTGFGGDYDLSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPVVEAGYVYI
HHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEE
AQPPIYGIKVGSETKEYIQPGENQEKDLQEALARWAQGRTKPTVQRYKGLGEMDDHQLWE
ECCCEEEEEECCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHH
TTMDPEHRLMARVSVEDAAEADKIFDMLMGDRVEPRREFIENNAQYSTIDA
CCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7608096; 11463916 [H]