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The map label for this gene is flpA [H]

Identifier: 125624362

GI number: 125624362

Start: 1531681

End: 1532370

Strand: Reverse

Name: flpA [H]

Synonym: llmg_1559

Alternate gene names: 125624362

Gene position: 1532370-1531681 (Counterclockwise)

Preceding gene: 125624363

Following gene: 125624361

Centisome position: 60.58

GC content: 36.81

Gene sequence:

>690_bases
ATGAAGATTAAAGATTTTGATGAGCATTTAAGTGACCACCATTGTATCCAGCTAGTCCCACTTTTTTCTGACTTGAGTCC
AGACGAGCTTGCTCAAGTTGAAGAAATTGTTCAACATAAAAAAGTCAAAAAAGGTGAAACGGTTATTCAACCATCGGCAG
AGCCACTTCTGACAATAGTTGCCAAAGGAAGTTTAAAAATATATCAAATCTCATCCACTGGAAAAGAACAGCTATTAAGA
GTGATTGAACCTGGTGGATATGAAGGAGAAAAATCTATTTTCGGCCTTGTCAATAAAAACTTATTTGGCGAAGCTCTATC
GGATAGTACGATTTGTTTTGTCAGAAAATCCGATTTTACGGAACTCTTACTCGCTAAACCTCAACTTTCCCTCCGTCTTC
TTGAAATGAACGCTGTGAAACAAAATGAAACGGAGCAACAAGCCAAGTTTTTGATGATGGAATCTGTAGAAATGCGTCTT
GCTAACTATTTATTGGATTTGGCTAAAATTACTGACGGATCTTATGTACAAATTCCAATGACTTTGAAGGATTTGTCAGC
ATTTATTGGCACGACTCCTGAAACAATCAGCCGAAAACTTCGTCTCTTAGAAGAGAAGGGATTGGTCGAGCATTACGGAA
AATCTTTCAAAATTTTAGATTATGAAAGACTTGAAGATGAATATGCTTAG

Upstream 100 bases:

>100_bases
GAACTTGCAATTCGCAAGCTTTACGGCTATAATTTGTCTATTATTCCCTATTTTGCAGGAGAACTTGGAAAAACGATAGG
CGAATTTATTGAGGAGGAAG

Downstream 100 bases:

>100_bases
ACAAATTTCTGTCAGTAAATTTATTTACTGACGGAAAAAATATAATCAAAAAAATACTGACGAATTGATTCTGTCAGTAT
TTTATTTTTAATGAAAAAAT

Product: FNR like protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKIKDFDEHLSDHHCIQLVPLFSDLSPDELAQVEEIVQHKKVKKGETVIQPSAEPLLTIVAKGSLKIYQISSTGKEQLLR
VIEPGGYEGEKSIFGLVNKNLFGEALSDSTICFVRKSDFTELLLAKPQLSLRLLEMNAVKQNETEQQAKFLMMESVEMRL
ANYLLDLAKITDGSYVQIPMTLKDLSAFIGTTPETISRKLRLLEEKGLVEHYGKSFKILDYERLEDEYA

Sequences:

>Translated_229_residues
MKIKDFDEHLSDHHCIQLVPLFSDLSPDELAQVEEIVQHKKVKKGETVIQPSAEPLLTIVAKGSLKIYQISSTGKEQLLR
VIEPGGYEGEKSIFGLVNKNLFGEALSDSTICFVRKSDFTELLLAKPQLSLRLLEMNAVKQNETEQQAKFLMMESVEMRL
ANYLLDLAKITDGSYVQIPMTLKDLSAFIGTTPETISRKLRLLEEKGLVEHYGKSFKILDYERLEDEYA
>Mature_229_residues
MKIKDFDEHLSDHHCIQLVPLFSDLSPDELAQVEEIVQHKKVKKGETVIQPSAEPLLTIVAKGSLKIYQISSTGKEQLLR
VIEPGGYEGEKSIFGLVNKNLFGEALSDSTICFVRKSDFTELLLAKPQLSLRLLEMNAVKQNETEQQAKFLMMESVEMRL
ANYLLDLAKITDGSYVQIPMTLKDLSAFIGTTPETISRKLRLLEEKGLVEHYGKSFKILDYERLEDEYA

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=186, Percent_Identity=25.8064516129032, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787595, Length=223, Percent_Identity=26.0089686098655, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 25965; Mature: 25965

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKDFDEHLSDHHCIQLVPLFSDLSPDELAQVEEIVQHKKVKKGETVIQPSAEPLLTIV
CCCCCHHHHCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
AKGSLKIYQISSTGKEQLLRVIEPGGYEGEKSIFGLVNKNLFGEALSDSTICFVRKSDFT
ECCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
ELLLAKPQLSLRLLEMNAVKQNETEQQAKFLMMESVEMRLANYLLDLAKITDGSYVQIPM
HHHHCCCCHHEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TLKDLSAFIGTTPETISRKLRLLEEKGLVEHYGKSFKILDYERLEDEYA
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCC
>Mature Secondary Structure
MKIKDFDEHLSDHHCIQLVPLFSDLSPDELAQVEEIVQHKKVKKGETVIQPSAEPLLTIV
CCCCCHHHHCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
AKGSLKIYQISSTGKEQLLRVIEPGGYEGEKSIFGLVNKNLFGEALSDSTICFVRKSDFT
ECCCEEEEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCHH
ELLLAKPQLSLRLLEMNAVKQNETEQQAKFLMMESVEMRLANYLLDLAKITDGSYVQIPM
HHHHCCCCHHEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TLKDLSAFIGTTPETISRKLRLLEEKGLVEHYGKSFKILDYERLEDEYA
HHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHCCCCEEEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]