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The map label for this gene is nucA [H]

Identifier: 125624285

GI number: 125624285

Start: 1453908

End: 1454675

Strand: Reverse

Name: nucA [H]

Synonym: llmg_1479

Alternate gene names: 125624285

Gene position: 1454675-1453908 (Counterclockwise)

Preceding gene: 125624286

Following gene: 125624284

Centisome position: 57.51

GC content: 30.34

Gene sequence:

>768_bases
ATGAAACTTACCATTTTACATACAAATAATGTTATTTTGAACTCTAATAATCATTATTCTTTGATAGATGAAATTAAGGA
GAGAAATAAAAAAACCGCAGAGGAAACATTATTTCTAGGAGCTACTGATATAAATTTTAGTAAGTCAAATTATCAAAATG
ATGAAAAAATTTTTTCAAAAAAGCTTAAATATGATGCGATAGCAATAGGGGAACATCAATTTGATGAAGGCTCACAAGGT
CTTGCTAAATTTCTCAAGCAATTAGATTTTCCAGTTTTAGCTGCGAATGTCCATATTGAAAAAGATGAAATATTAAATAC
TTTTGAAAAAAATGGAAAATTTTTACCTTATTTACTGAAAGAAAGTAAATTTGGCAAGATTGGGATTTTTGGACTGACTC
CAATGAGTACGGTTTACGATTCTTGCCCGAGTTCGGACACGATTTTTACCAATCCTTCTGACAGAACTGAGTTTATTGTA
AAAATGCTAAAAAAGAAAGAAGCAAATCTTATTATTCTACTCAGTCAACTGGGGCAAGATGAGAATCAAATTTTAGCTCA
AAAATTTCCTGAAATTGATGTCATCATTGACAGAGCAACTTATAAGACCGATAAAACAATTAATAAATGGAAAGAAACAC
TTATGGTCCAGACAATGGCTGATTTTCCTTCCATCTTTGAATTAGAGCTAGAAGTTGATGAAGAAGGAAAGTTACTGACA
GCTCAGGAAAAATATCATGAAGTATTTGAAAGTGTTAATTATGGATAA

Upstream 100 bases:

>100_bases
TAGTCATTTTTTTTATAGAAAATGTTCAAGAAACACAATATATGGTATTTTTGTGGTAAAATGTAAGTATTAAATACTAT
ATATAGGGTTGGTTATAATA

Downstream 100 bases:

>100_bases
GTTTATTTTATCTTGACAAAATGAGAAAATAGCGCTAGAATTTATAGGTAGCGACTCGAAGATAGTGAAAGTTCGAGAAA
GAAAATGGCTTAATTTAAAA

Product: 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKLTILHTNNVILNSNNHYSLIDEIKERNKKTAEETLFLGATDINFSKSNYQNDEKIFSKKLKYDAIAIGEHQFDEGSQG
LAKFLKQLDFPVLAANVHIEKDEILNTFEKNGKFLPYLLKESKFGKIGIFGLTPMSTVYDSCPSSDTIFTNPSDRTEFIV
KMLKKKEANLIILLSQLGQDENQILAQKFPEIDVIIDRATYKTDKTINKWKETLMVQTMADFPSIFELELEVDEEGKLLT
AQEKYHEVFESVNYG

Sequences:

>Translated_255_residues
MKLTILHTNNVILNSNNHYSLIDEIKERNKKTAEETLFLGATDINFSKSNYQNDEKIFSKKLKYDAIAIGEHQFDEGSQG
LAKFLKQLDFPVLAANVHIEKDEILNTFEKNGKFLPYLLKESKFGKIGIFGLTPMSTVYDSCPSSDTIFTNPSDRTEFIV
KMLKKKEANLIILLSQLGQDENQILAQKFPEIDVIIDRATYKTDKTINKWKETLMVQTMADFPSIFELELEVDEEGKLLT
AQEKYHEVFESVNYG
>Mature_255_residues
MKLTILHTNNVILNSNNHYSLIDEIKERNKKTAEETLFLGATDINFSKSNYQNDEKIFSKKLKYDAIAIGEHQFDEGSQG
LAKFLKQLDFPVLAANVHIEKDEILNTFEKNGKFLPYLLKESKFGKIGIFGLTPMSTVYDSCPSSDTIFTNPSDRTEFIV
KMLKKKEANLIILLSQLGQDENQILAQKFPEIDVIIDRATYKTDKTINKWKETLMVQTMADFPSIFELELEVDEEGKLLT
AQEKYHEVFESVNYG

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=218, Percent_Identity=27.5229357798165, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786687, Length=215, Percent_Identity=29.3023255813954, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24641187, Length=136, Percent_Identity=33.0882352941176, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI221329836, Length=136, Percent_Identity=33.0882352941176, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19922444, Length=222, Percent_Identity=27.027027027027, Blast_Score=68, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19922446, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24654424, Length=201, Percent_Identity=28.3582089552239, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 29250; Mature: 29250

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTILHTNNVILNSNNHYSLIDEIKERNKKTAEETLFLGATDINFSKSNYQNDEKIFSK
CEEEEEECCCEEEECCCCCHHHHHHHHHCHHHHHHEEEEEECCCCCCCCCCCCHHHHHHH
KLKYDAIAIGEHQFDEGSQGLAKFLKQLDFPVLAANVHIEKDEILNTFEKNGKFLPYLLK
HHCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHCCCEEEHHHC
ESKFGKIGIFGLTPMSTVYDSCPSSDTIFTNPSDRTEFIVKMLKKKEANLIILLSQLGQD
CCCCCEEEEEECCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCC
ENQILAQKFPEIDVIIDRATYKTDKTINKWKETLMVQTMADFPSIFELELEVDEEGKLLT
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
AQEKYHEVFESVNYG
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKLTILHTNNVILNSNNHYSLIDEIKERNKKTAEETLFLGATDINFSKSNYQNDEKIFSK
CEEEEEECCCEEEECCCCCHHHHHHHHHCHHHHHHEEEEEECCCCCCCCCCCCHHHHHHH
KLKYDAIAIGEHQFDEGSQGLAKFLKQLDFPVLAANVHIEKDEILNTFEKNGKFLPYLLK
HHCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEECHHHHHHHHHHCCCEEEHHHC
ESKFGKIGIFGLTPMSTVYDSCPSSDTIFTNPSDRTEFIVKMLKKKEANLIILLSQLGQD
CCCCCEEEEEECCHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCEEEEEHHHCCC
ENQILAQKFPEIDVIIDRATYKTDKTINKWKETLMVQTMADFPSIFELELEVDEEGKLLT
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCEEE
AQEKYHEVFESVNYG
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]