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The map label for this gene is glyS [H]

Identifier: 125624283

GI number: 125624283

Start: 1450705

End: 1452726

Strand: Reverse

Name: glyS [H]

Synonym: llmg_1477

Alternate gene names: 125624283

Gene position: 1452726-1450705 (Counterclockwise)

Preceding gene: 125624284

Following gene: 125624282

Centisome position: 57.43

GC content: 35.21

Gene sequence:

>2022_bases
ATGACAAATTACTTACTTGAAATTGGTTTGGAAGAAATTCCAGCACATTTGGTGACTCCTTCAATCAATCAATTGGCTGA
ACGAATGGAAGCTTTTCTTAATGAAAACCGTTTGAAATTTGATAAAATCATTAAATTTTCAACACCACGCCGCCTAGCTC
TTATTGTTGAAGAATTGTCAGACTCATCAGAAGCGATTGATGAAGAAGTTAAAGGTCCTTCTGCAAAAATTGCTAAAGAC
GCTGAAGGAAATTGGTCAAAAGCGATTCAAGGTTTTTCACGTGGTCAAGGAGCGACTCCGGATGATTTAATCCTTAAGGG
TGATTATTATTATGCTAAAAAACATGTTGATGGTGTAAAATCAGAAGAAATTCTTTCTAAAGTTGGGGATGAAGTCATTG
CAAAAATGACCTTCTCAACTTATATGAAATGGGGAAATAATGATTTCTTGTTTGTTCGTCCTATACAATGGATCGTATCA
CTTTTAGAAGATGAAATCGTTGCTTTTGATTTATTAGATGTGACCGCCAATCGTTTTTCACGTGGTCATCGTTTCTTGGC
AAATGTTGAGATTGAACTCAAAAATGCCAATGATTATGCTAGCAAAATGCCAGAAAATTTTGTTTTAGTTGATGCTGAAC
ATCGTAAAGCCGAAATATCTGCACAAATTTTGGCTCTTGCATCTGAAAATAATTGGCAAGTTACTTTGCATAAAGATTTA
TTAGAAGAAGTAAATAATATTGTTGAATATCCAACAGCCTTTGTAGGCTCATTTGATCCAAAATATTTGTCTGTTCCAGC
AGAAGTTTTAGTGACATCAATGCGTGATAATCAACGTTATTTTGAAGTTTATAACCAAGAAGGTCAATTGGCTCCTAACT
TTATTTCGGTTCGTAATGGGAATGCCGAACATATTGAAAATGTAGTGCTTGGAAATGAAAAAGTCTTGTTTGCTCGTCTT
GAAGATGCTGAATTCTTCTGGAAAGAAGACCAAAAACTTAAGATTGAAGACTTAGTGGCTAAACTTGCTAAAGTAACTTT
CCATGCAAAAATTGGTTCAATCACTGAACATATGGCACGAACAAAATTAATTGCTGCTAAATTAGCTGATATTGCTGGTT
TAACAGATGAAGAAAAAGTAGACGTGGCTCGTAGCGCTGAAATTTATAAATTTGACCTTTTGACAGGAATGGTTGGAGAA
TTTGATGAATTGCAAGGCGTGATGGGTGAAAAATACGCTCTTTTAGCAGGGGAAAATGCAAATGTTGCTGCGGCTATTCG
TGAACATTACATGCCGACCTCAGCTGACGGCCAATTACCTGAAACAAAAGTTGGTTCTGTTCTTGCAGCAGCAGATAAAA
TTGATTCAGTCCTTTCTTTCTTTAATGTTGGTTTGATTCCATCTGGTTCAAATGACCCTTATGCGCTACGTCGTGCAGTA
CAAGGATTGATTCGAATTATCGAAAAAATGAATTGGCATTTCGACCTATCTTTATTTATCGACCAATTTGAGGGTCAAAA
TCATGCTGAAATTTTGGAATTCGTCAAAGCACGCGTTCAAAAACTTTTGCTTGAAAAATTAGACCGTTATGATATTGTTG
AAGCAGCAATTAATAGCTCAAACTTTGATATTACAAATATGATGGAATCAGCATTTGTTATTGATGGTCATAAACTTCAC
GAACCATTTAAACCAGCAATTGAAAATGTTTCACGTTCAATTAATTTAGTTAAAAAAGCAGCTGATATTGCTGAAATTAA
TCCAGCACTTTTTGAAGAAGATACTGAACAAGCACTTTATGATGCCGTTATTTCTCTGCAAAATCAATGGACTTATAAGC
CATGTGAAGAAAAATTCCGAGCAATTGTTCATATGCTTGCTCCTGCAATTGAAGCTTTCTTTGATAATGTGATGGTTATG
GCCGAAGATTTAGCTGTTCGTGATAATCGGATTGCCCTATTGTCAGAAGTTGTTGCATTAACAAGTGTTATGGCTGATTT
TTCTTTGATTAATACAAAATAA

Upstream 100 bases:

>100_bases
CGAAACTTGGCTTCCCACTCTTAAAAGATGAAGCTTTGCGTGAAAAATACCTTGGTAAAAAAGGAAAATATACGAAGATT
GTAGAAGGAGAAGAAAAATA

Downstream 100 bases:

>100_bases
AGCTGACTCATTTACTGACATAGAGTTGCAAAGCCCTTTTAAATGCTGTCAGTAAAAATAAATTCTGTCAGTAATTTTAA
AAACATTTACGAAAAGGAAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTNYLLEIGLEEIPAHLVTPSINQLAERMEAFLNENRLKFDKIIKFSTPRRLALIVEELSDSSEAIDEEVKGPSAKIAKD
AEGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHVDGVKSEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVS
LLEDEIVAFDLLDVTANRFSRGHRFLANVEIELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENNWQVTLHKDL
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNGNAEHIENVVLGNEKVLFARL
EDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMARTKLIAAKLADIAGLTDEEKVDVARSAEIYKFDLLTGMVGE
FDELQGVMGEKYALLAGENANVAAAIREHYMPTSADGQLPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
QGLIRIIEKMNWHFDLSLFIDQFEGQNHAEILEFVKARVQKLLLEKLDRYDIVEAAINSSNFDITNMMESAFVIDGHKLH
EPFKPAIENVSRSINLVKKAADIAEINPALFEEDTEQALYDAVISLQNQWTYKPCEEKFRAIVHMLAPAIEAFFDNVMVM
AEDLAVRDNRIALLSEVVALTSVMADFSLINTK

Sequences:

>Translated_673_residues
MTNYLLEIGLEEIPAHLVTPSINQLAERMEAFLNENRLKFDKIIKFSTPRRLALIVEELSDSSEAIDEEVKGPSAKIAKD
AEGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHVDGVKSEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVS
LLEDEIVAFDLLDVTANRFSRGHRFLANVEIELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENNWQVTLHKDL
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNGNAEHIENVVLGNEKVLFARL
EDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMARTKLIAAKLADIAGLTDEEKVDVARSAEIYKFDLLTGMVGE
FDELQGVMGEKYALLAGENANVAAAIREHYMPTSADGQLPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
QGLIRIIEKMNWHFDLSLFIDQFEGQNHAEILEFVKARVQKLLLEKLDRYDIVEAAINSSNFDITNMMESAFVIDGHKLH
EPFKPAIENVSRSINLVKKAADIAEINPALFEEDTEQALYDAVISLQNQWTYKPCEEKFRAIVHMLAPAIEAFFDNVMVM
AEDLAVRDNRIALLSEVVALTSVMADFSLINTK
>Mature_672_residues
TNYLLEIGLEEIPAHLVTPSINQLAERMEAFLNENRLKFDKIIKFSTPRRLALIVEELSDSSEAIDEEVKGPSAKIAKDA
EGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHVDGVKSEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVSL
LEDEIVAFDLLDVTANRFSRGHRFLANVEIELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENNWQVTLHKDLL
EEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNGNAEHIENVVLGNEKVLFARLE
DAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMARTKLIAAKLADIAGLTDEEKVDVARSAEIYKFDLLTGMVGEF
DELQGVMGEKYALLAGENANVAAAIREHYMPTSADGQLPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAVQ
GLIRIIEKMNWHFDLSLFIDQFEGQNHAEILEFVKARVQKLLLEKLDRYDIVEAAINSSNFDITNMMESAFVIDGHKLHE
PFKPAIENVSRSINLVKKAADIAEINPALFEEDTEQALYDAVISLQNQWTYKPCEEKFRAIVHMLAPAIEAFFDNVMVMA
EDLAVRDNRIALLSEVVALTSVMADFSLINTK

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=696, Percent_Identity=33.4770114942529, Blast_Score=351, Evalue=7e-98,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75643; Mature: 75512

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNYLLEIGLEEIPAHLVTPSINQLAERMEAFLNENRLKFDKIIKFSTPRRLALIVEELS
CCCHHEECCHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHC
DSSEAIDEEVKGPSAKIAKDAEGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHVDGVK
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCEEEHHHHCCCCC
SEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVSLLEDEIVAFDLLDVTANRFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGHRFLANVEIELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENNWQVTLHKDL
CCCEEEEEEEEEEECCCHHHHHCCCCEEEEECCCCHHHHHEEEEEEECCCCCEEEEHHHH
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNG
HHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCC
NAEHIENVVLGNEKVLFARLEDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMAR
CHHHHHHHHCCCCEEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TKLIAAKLADIAGLTDEEKVDVARSAEIYKFDLLTGMVGEFDELQGVMGEKYALLAGENA
HHHHHHHHHHHHCCCCCHHHHHHHCCCCEEHHHHHHHHCCHHHHHHHCCCCEEEEECCCC
NVAAAIREHYMPTSADGQLPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QGLIRIIEKMNWHFDLSLFIDQFEGQNHAEILEFVKARVQKLLLEKLDRYDIVEAAINSS
HHHHHHHHHCCCCEEHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
NFDITNMMESAFVIDGHKLHEPFKPAIENVSRSINLVKKAADIAEINPALFEEDTEQALY
CCCHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
DAVISLQNQWTYKPCEEKFRAIVHMLAPAIEAFFDNVMVMAEDLAVRDNRIALLSEVVAL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TSVMADFSLINTK
HHHHHHHHHHCCC
>Mature Secondary Structure 
TNYLLEIGLEEIPAHLVTPSINQLAERMEAFLNENRLKFDKIIKFSTPRRLALIVEELS
CCHHEECCHHHCCHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHHHHHHC
DSSEAIDEEVKGPSAKIAKDAEGNWSKAIQGFSRGQGATPDDLILKGDYYYAKKHVDGVK
CCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEECCEEEHHHHCCCCC
SEEILSKVGDEVIAKMTFSTYMKWGNNDFLFVRPIQWIVSLLEDEIVAFDLLDVTANRFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGHRFLANVEIELKNANDYASKMPENFVLVDAEHRKAEISAQILALASENNWQVTLHKDL
CCCEEEEEEEEEEECCCHHHHHCCCCEEEEECCCCHHHHHEEEEEEECCCCCEEEEHHHH
LEEVNNIVEYPTAFVGSFDPKYLSVPAEVLVTSMRDNQRYFEVYNQEGQLAPNFISVRNG
HHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCEEEECCC
NAEHIENVVLGNEKVLFARLEDAEFFWKEDQKLKIEDLVAKLAKVTFHAKIGSITEHMAR
CHHHHHHHHCCCCEEEEEEECCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHH
TKLIAAKLADIAGLTDEEKVDVARSAEIYKFDLLTGMVGEFDELQGVMGEKYALLAGENA
HHHHHHHHHHHHCCCCCHHHHHHHCCCCEEHHHHHHHHCCHHHHHHHCCCCEEEEECCCC
NVAAAIREHYMPTSADGQLPETKVGSVLAAADKIDSVLSFFNVGLIPSGSNDPYALRRAV
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
QGLIRIIEKMNWHFDLSLFIDQFEGQNHAEILEFVKARVQKLLLEKLDRYDIVEAAINSS
HHHHHHHHHCCCCEEHEEHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
NFDITNMMESAFVIDGHKLHEPFKPAIENVSRSINLVKKAADIAEINPALFEEDTEQALY
CCCHHHHHHHHEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHH
DAVISLQNQWTYKPCEEKFRAIVHMLAPAIEAFFDNVMVMAEDLAVRDNRIALLSEVVAL
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
TSVMADFSLINTK
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]