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The map label for this gene is nadE [H]

Identifier: 125624277

GI number: 125624277

Start: 1444713

End: 1445537

Strand: Reverse

Name: nadE [H]

Synonym: llmg_1470

Alternate gene names: 125624277

Gene position: 1445537-1444713 (Counterclockwise)

Preceding gene: 125624278

Following gene: 125624276

Centisome position: 57.15

GC content: 38.91

Gene sequence:

>825_bases
ATGACATTACAAGAAGAAATTATTAAAGAACTTGGTGTGAAACCAATTATTGATCCTAAAGAAGAAATTAGAGTGTCAGT
TGATTTTTTGAAAGATTATCTTAAAAAGTATCCATTCATCAAATCATTTGTCCTTGGAATTTCTGGCGGACAAGATTCAA
GTTTAGCAGGCCGCTTAGCTCAGATTGCTATCGAAGAAATGCGACAAGAAACAGCTGATGCTTCTTATAAATTTGTTGCT
GTACGTTTGCCTTTTGGTGTGCAAGCCGATGAAGAAGATGCTCAACGTGCGCTTGCCTTTATTAAGCCAGATGTCAGTTT
GGCAGTAAATATCAAAGCAGCTGTTGAAGGACAAGTAGCGGCTCTAAATGAAGCAGGTGTTGAGGTTTCAGACTTCAATA
AAGGAAATATTAAAGCCCGTCAACGTATGATTACCCAATATGCTGTGGCTGGACAATATCAAGGTGCTGTTCTTGGAACA
GACCATGCAGCAGAAAATATTACTGGATTTTTCACGAAATTTGGTGATGGCGGAGCTGACTTATTGCCACTTTTCCGTTT
GAATAAACGTCAAGGTAAAGCACTTTTGGCAGAACTTGGTGCTGATCCAGCAATCTATGAAAAAGTTCCAACTGCTGATC
TTGAAGAAGGTAAACCTGGACTTGCCGACGAGATTGCACTTGGTGTGACTTACAATGATATTGATGACTATACAGAAGGA
AAAGTGATTTCTGATGATGCCAAGGCCAAAATCGAAGCTTGGTGGAATAAAACGCAACATAAACGTCACTTACCAATCAG
TATCTTTGATGATTTCTGGAAATAA

Upstream 100 bases:

>100_bases
GAGAATTTATTGATGAATTTTACATGAGTAAAATTTTAGATTAAAAGTTGTTGTGGAGCTGTCAGTGAAATTTTATAGCT
GTCAGTGAAAGGAAGAGAAA

Downstream 100 bases:

>100_bases
AAATAAATATAATTACTGACAGTTCAACACGACCAAAGTGAGTGTTGAATCGGTGGATAAAAAGTTACTGACGGGAGTTT
CGTCAGTAACTTTTTATTTC

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTLQEEIIKELGVKPIIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLAQIAIEEMRQETADASYKFVA
VRLPFGVQADEEDAQRALAFIKPDVSLAVNIKAAVEGQVAALNEAGVEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGT
DHAAENITGFFTKFGDGGADLLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
KVISDDAKAKIEAWWNKTQHKRHLPISIFDDFWK

Sequences:

>Translated_274_residues
MTLQEEIIKELGVKPIIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLAQIAIEEMRQETADASYKFVA
VRLPFGVQADEEDAQRALAFIKPDVSLAVNIKAAVEGQVAALNEAGVEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGT
DHAAENITGFFTKFGDGGADLLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
KVISDDAKAKIEAWWNKTQHKRHLPISIFDDFWK
>Mature_273_residues
TLQEEIIKELGVKPIIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLAQIAIEEMRQETADASYKFVAV
RLPFGVQADEEDAQRALAFIKPDVSLAVNIKAAVEGQVAALNEAGVEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGTD
HAAENITGFFTKFGDGGADLLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEGK
VISDDAKAKIEAWWNKTQHKRHLPISIFDDFWK

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1788036, Length=274, Percent_Identity=63.8686131386861, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729 [H]

Pfam domain/function: PF02540 NAD_synthase [H]

EC number: =6.3.1.5 [H]

Molecular weight: Translated: 30150; Mature: 30018

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQEEIIKELGVKPIIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLA
CCCHHHHHHHCCCCCCCCCHHHHEEEHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHH
QIAIEEMRQETADASYKFVAVRLPFGVQADEEDAQRALAFIKPDVSLAVNIKAAVEGQVA
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCEE
ALNEAGVEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGTDHAAENITGFFTKFGDGGAD
EHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
LLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
HHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCC
KVISDDAKAKIEAWWNKTQHKRHLPISIFDDFWK
CEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure 
TLQEEIIKELGVKPIIDPKEEIRVSVDFLKDYLKKYPFIKSFVLGISGGQDSSLAGRLA
CCHHHHHHHCCCCCCCCCHHHHEEEHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHH
QIAIEEMRQETADASYKFVAVRLPFGVQADEEDAQRALAFIKPDVSLAVNIKAAVEGQVA
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCEE
ALNEAGVEVSDFNKGNIKARQRMITQYAVAGQYQGAVLGTDHAAENITGFFTKFGDGGAD
EHHHCCCEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHCCCCCCC
LLPLFRLNKRQGKALLAELGADPAIYEKVPTADLEEGKPGLADEIALGVTYNDIDDYTEG
HHHHHHCCCHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCHHHEEEEEEECCCCCCCCC
KVISDDAKAKIEAWWNKTQHKRHLPISIFDDFWK
CEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]