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The map label for this gene is pcrA [H]

Identifier: 125624253

GI number: 125624253

Start: 1410489

End: 1412765

Strand: Reverse

Name: pcrA [H]

Synonym: llmg_1445

Alternate gene names: 125624253

Gene position: 1412765-1410489 (Counterclockwise)

Preceding gene: 125624255

Following gene: 125624252

Centisome position: 55.85

GC content: 37.15

Gene sequence:

>2277_bases
ATGAATCCATTATTACAGGGAATGAACGAAAAACAAGCACAAGCGGTCCAAACGACAGAAGGACCTTTATTAATTATGGC
TGGAGCTGGTTCAGGGAAGACTCGTGTCTTAACTCACCGGATTGCTTATTTAATTGATGAAAAAATGGTTAATCCTTGGA
ATATACTAGCGATTACCTTTACAAATAAAGCAGCAAAAGAAATGCGGGAGCGTGCATTATCACTTACTCCTAGAGCACAA
GATACATTAATTGCAACTTTTCACTCAATGTGTGTCCGAATTTTAAGGCGTGATGCTGACCATATTGGCTATAATCGTAA
TTTTACAATTATTGACCCAGGTGATCAAAAATCATTGATGAAACGGATTTTGAAAGAAGCTAATTTGGATCCTAAAAAAT
GGGAACCTAAAGGTCTTTTAAATGCAATTTCAAATGCTAAAAATGACTTACTAGATGAAACAGCTTATGAAGCACAAATT
ACTGCTAGACACCCTTATGAAATGGTTGTTGCGCGTGTTTATAAGATTTACCAAAAGGAATTGCGCAAAGCAGAATCAAT
GGATTTTGATGATTTAATCATGCAAACCTTACGTCTTTTTGATGAAAATCCTGATGTTTTAGCCTATTATCAAGGGAAAT
TTCAATATATCCATGTTGATGAATATCAAGATACCAACCATGCTCAATATCAATTAGTCAAACTATTGGCTAGTCGTTTT
CAAAATATTTGTGTTGTTGGGGACGCTGACCAATCGATTTATGGTTGGCGTGGAGCTGATATGCAAAATATTTTAGATTT
TGAGAAAGATTATCCAAAAGCTAAAGTCGTCCTATTGGAAGAAAACTATCGTTCGACTAAGACCATACTTGATGCAGCTA
ATAATGTTATCAAAAACAACTTGAAAAGACGCCCTAAAGAACTATGGACTCAGAATGATGAAGGAGATAAGATTGTTTAT
TTCCGAGCAGGAAATGAGCAAGAAGAAGCAAATAAAGTTTCTGAAATCATTAGTTCAGGAGTTCGTTCAGGTCGTTCATA
TGCTGATTATGCGGTTCTCTATCGAACCAATGCTCAATCTCGGACGATTGAAGATTCGTTTGTGAAATCAAATATTCCTT
ATACGATGGTTGCAGGAACAAAATTTTATTCTCGTAAAGAAATTCGAGATGTGATTGCTTATTTAAATGTAGTAGCCAAT
CCAGCGGATAATATGAGTTTTGAACGGATTGTCAATGAACCTAAAAGAGGAGTTGGTCCGAGGGCTTTAGAAAAATTACG
TCGCTTTGCAGAAAGTCGTAATCTTTCTTTAGCAGATTCAAGCTTTGATATTATGCTTTCTGATATTCGAGGAAAAGCGG
CTGGCGAGATTTATAATTTAGCAATGGCCTTTGATTTGTTGAGAACACATAGCGAAAGTTCAACAATCACAGAATTGGTT
GAAGAAATGTTAGAGCGTACGGGTTATCTTAAGGCCTTGCAGCTAATGCCAAGTATTGAAAATCAGGCTCGGATTGAAAA
TATTGAAGAATTCTTATCTGTAACCAAGGGTTTTGACGAGAAATCAGAAGTTCCTTTGGATGAAGAAACTGGTGAGCCTA
TCATTGAAACAGGTTTGGACAAATTGAGCCGTTTCCTTAATGAAGTCAGCTTGCTTTCTGAAACAGATGCCTACGAGGAA
GAATCTGACCAAGTCACAATGATGACTTTGCATGCCGCAAAAGGTTTAGAGTTTCCTATTGTTTTCTTAATCGGGATGGA
AGAAAATATTTTTCCGTTGTCACGTGTGAATGAAGATATTGATGAGTTGGAAGAAGAACGGCGACTAGCCTATGTTGGTA
TTACAAGAGCAGAAGAAACGCTTTATCTAATGAATGCCAATCAACGGGTTCTTTATGGGAAAACTAGCTATAATCGCCCT
AGTCGTTTTATTTCTGAAATTGATGATGAACTTCTTGATTATGCTGGGATTGCTCGTAAAGCAAATTCAACATATAATGC
TTCTTATAAATCAGGTGGTTTTGCAAGTGGAATCTCAATGTCAGATGCACTTCATCAGCGTAAGGCAGTGATTAATCCAA
CTGTTGGTCAATCAATCATTGACAACTCAGAAACCGAAGATTGGCAAATCGGGGACACCGCCATTCATCGTAAATGGGGC
GAAGGAATGGTCTTAGGGATTTCAGGTTCTGGTAAAAACATGGAACTTAAGATTAATTTCCCAGAGGTAGGAATGAAGCG
TCTTTTAGCAGCAATGGCGCCTATTGAGAAAAAATAA

Upstream 100 bases:

>100_bases
TCACAAGCTTATTTGTGAAATAAAGGAGTGCCAAAAGAACTTCATGATGTGTTTGACTTATGCTATAATTAATAGTGATT
GTGACAGCTAGGAGAATAAA

Downstream 100 bases:

>100_bases
GTAACAAAATTATTGGAGAAACTAATGGCTTATCATGAGTTAGAAAAAGTCGAATTGACAAATATGTGTGCCATTATTGA
CGAAAAAAATCAAAAAGTTC

Product: ATP-dependent DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MNPLLQGMNEKQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITFTNKAAKEMRERALSLTPRAQ
DTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLMKRILKEANLDPKKWEPKGLLNAISNAKNDLLDETAYEAQI
TARHPYEMVVARVYKIYQKELRKAESMDFDDLIMQTLRLFDENPDVLAYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNNLKRRPKELWTQNDEGDKIVY
FRAGNEQEEANKVSEIISSGVRSGRSYADYAVLYRTNAQSRTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVAN
PADNMSFERIVNEPKRGVGPRALEKLRRFAESRNLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
EEMLERTGYLKALQLMPSIENQARIENIEEFLSVTKGFDEKSEVPLDEETGEPIIETGLDKLSRFLNEVSLLSETDAYEE
ESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDIDELEEERRLAYVGITRAEETLYLMNANQRVLYGKTSYNRP
SRFISEIDDELLDYAGIARKANSTYNASYKSGGFASGISMSDALHQRKAVINPTVGQSIIDNSETEDWQIGDTAIHRKWG
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK

Sequences:

>Translated_758_residues
MNPLLQGMNEKQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITFTNKAAKEMRERALSLTPRAQ
DTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLMKRILKEANLDPKKWEPKGLLNAISNAKNDLLDETAYEAQI
TARHPYEMVVARVYKIYQKELRKAESMDFDDLIMQTLRLFDENPDVLAYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNNLKRRPKELWTQNDEGDKIVY
FRAGNEQEEANKVSEIISSGVRSGRSYADYAVLYRTNAQSRTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVAN
PADNMSFERIVNEPKRGVGPRALEKLRRFAESRNLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
EEMLERTGYLKALQLMPSIENQARIENIEEFLSVTKGFDEKSEVPLDEETGEPIIETGLDKLSRFLNEVSLLSETDAYEE
ESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDIDELEEERRLAYVGITRAEETLYLMNANQRVLYGKTSYNRP
SRFISEIDDELLDYAGIARKANSTYNASYKSGGFASGISMSDALHQRKAVINPTVGQSIIDNSETEDWQIGDTAIHRKWG
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK
>Mature_758_residues
MNPLLQGMNEKQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITFTNKAAKEMRERALSLTPRAQ
DTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLMKRILKEANLDPKKWEPKGLLNAISNAKNDLLDETAYEAQI
TARHPYEMVVARVYKIYQKELRKAESMDFDDLIMQTLRLFDENPDVLAYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNNLKRRPKELWTQNDEGDKIVY
FRAGNEQEEANKVSEIISSGVRSGRSYADYAVLYRTNAQSRTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVAN
PADNMSFERIVNEPKRGVGPRALEKLRRFAESRNLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
EEMLERTGYLKALQLMPSIENQARIENIEEFLSVTKGFDEKSEVPLDEETGEPIIETGLDKLSRFLNEVSLLSETDAYEE
ESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDIDELEEERRLAYVGITRAEETLYLMNANQRVLYGKTSYNRP
SRFISEIDDELLDYAGIARKANSTYNASYKSGGFASGISMSDALHQRKAVINPTVGQSIIDNSETEDWQIGDTAIHRKWG
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=757, Percent_Identity=37.5165125495377, Blast_Score=496, Evalue=1e-141,
Organism=Escherichia coli, GI48994965, Length=652, Percent_Identity=40.1840490797546, Blast_Score=423, Evalue=1e-119,
Organism=Escherichia coli, GI1787196, Length=332, Percent_Identity=25.9036144578313, Blast_Score=91, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322369, Length=737, Percent_Identity=30.393487109905, Blast_Score=232, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324477, Length=679, Percent_Identity=23.4167893961708, Blast_Score=117, Evalue=5e-27,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 86086; Mature: 86086

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLLQGMNEKQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITF
CCHHHCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAKEMRERALSLTPRAQDTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLM
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEECCCCHHHHH
KRILKEANLDPKKWEPKGLLNAISNAKNDLLDETAYEAQITARHPYEMVVARVYKIYQKE
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHH
LRKAESMDFDDLIMQTLRLFDENPDVLAYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHH
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNN
CCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LKRRPKELWTQNDEGDKIVYFRAGNEQEEANKVSEIISSGVRSGRSYADYAVLYRTNAQS
HHHCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVANPADNMSFERIVNEPKRGVGP
CCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCH
RALEKLRRFAESRNLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
HHHHHHHHHHHHCCCEECCCCHHHHEEHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EEMLERTGYLKALQLMPSIENQARIENIEEFLSVTKGFDEKSEVPLDEETGEPIIETGLD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
KLSRFLNEVSLLSETDAYEEESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDI
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCHHHHCHHH
DELEEERRLAYVGITRAEETLYLMNANQRVLYGKTSYNRPSRFISEIDDELLDYAGIARK
HHHHHHCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ANSTYNASYKSGGFASGISMSDALHQRKAVINPTVGQSIIDNSETEDWQIGDTAIHRKWG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHCC
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK
CEEEEEEECCCCCEEEEEECCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNPLLQGMNEKQAQAVQTTEGPLLIMAGAGSGKTRVLTHRIAYLIDEKMVNPWNILAITF
CCHHHCCCCHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEEEE
TNKAAKEMRERALSLTPRAQDTLIATFHSMCVRILRRDADHIGYNRNFTIIDPGDQKSLM
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEECCCCHHHHH
KRILKEANLDPKKWEPKGLLNAISNAKNDLLDETAYEAQITARHPYEMVVARVYKIYQKE
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHH
LRKAESMDFDDLIMQTLRLFDENPDVLAYYQGKFQYIHVDEYQDTNHAQYQLVKLLASRF
HHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECEEEEEEECCCCCCCHHHHHHHHHHHHHH
QNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVLLEENYRSTKTILDAANNVIKNN
CCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHH
LKRRPKELWTQNDEGDKIVYFRAGNEQEEANKVSEIISSGVRSGRSYADYAVLYRTNAQS
HHHCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCC
RTIEDSFVKSNIPYTMVAGTKFYSRKEIRDVIAYLNVVANPADNMSFERIVNEPKRGVGP
CCHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCH
RALEKLRRFAESRNLSLADSSFDIMLSDIRGKAAGEIYNLAMAFDLLRTHSESSTITELV
HHHHHHHHHHHHCCCEECCCCHHHHEEHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EEMLERTGYLKALQLMPSIENQARIENIEEFLSVTKGFDEKSEVPLDEETGEPIIETGLD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
KLSRFLNEVSLLSETDAYEEESDQVTMMTLHAAKGLEFPIVFLIGMEENIFPLSRVNEDI
HHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHCCCCCCEEEEEECCCCCCCHHHHCHHH
DELEEERRLAYVGITRAEETLYLMNANQRVLYGKTSYNRPSRFISEIDDELLDYAGIARK
HHHHHHCCEEEEEEECCCCEEEEEECCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ANSTYNASYKSGGFASGISMSDALHQRKAVINPTVGQSIIDNSETEDWQIGDTAIHRKWG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHCC
EGMVLGISGSGKNMELKINFPEVGMKRLLAAMAPIEKK
CEEEEEEECCCCCEEEEEECCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA