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The map label for this gene is truB

Identifier: 125624242

GI number: 125624242

Start: 1398755

End: 1399747

Strand: Reverse

Name: truB

Synonym: llmg_1431

Alternate gene names: 125624242

Gene position: 1399747-1398755 (Counterclockwise)

Preceding gene: 125624243

Following gene: 125624241

Centisome position: 55.34

GC content: 36.76

Gene sequence:

>993_bases
ATGAATGAAAATTTGAACGGCATTTTAAATGTCTATAAAGAAGCTGGTTGGACATCTTTTGATGTCGTCGCCAAACTTCG
TGGTATTTTAAAAACAAAAAAAATTGGACATGGTGGGACTTTAGATCCATCAGTAACCGGTGTTTTACCTATTGCTGTTG
GAAAATCAACACGATTATTAGAATACATGGAAGCAGCAGGTAAAATTTACGAAGGTCAAGTGACGATTGGGTTTTCAACT
GAAACCGAGGATGCTGATGGTGCAATTGTAAATCAAACGCCAGTAAAAAATAATTTAACTGAGAGTGAAATTGATTCTGC
TATGTCGCACTTTGTCGGGAAAATAAAACAGATTCCGCCAATGTATTCGGCAGTAAAAATCAATGGGAAAAAGTTATATG
AATATGCTAGAGCGGGTCAGACGATTGAACGTCCAGCAAGAGAGATTACAATAAAATCTTTTGTAAGGACAAGCCCGATT
GATTGGAATAAGGAAGAAGGCTTAGTCACTTTTTCTTTCAAAGTTGAGTGTAGTAAAGGGACTTATGTTCGGACTTTAGC
GGTTGATTTGGCGGACAGTCTTGGCTATGCAGGACATATGTCAAAATTACAACGGACGGCAAGTAATGGCTTACTGATTA
AAGATGCTATTAAATTGTCAAAAATTGAAGAAATAAAAGAATCGGGAAAGCTTTCTACTATATTGTATCCAGCAGAATAT
GCTGTCAGTGATTTGCCTAGAGTTAATTTAACTACTGTTCAGTTTGATATGGCGCGTGTTGGCAAGAAATTTGCTCAAAG
TGATTGGACAAATGAGGTAGAAACTGCTGACAGCTCTGTCAACAAGACAAAGCAGCAAGAATATTTACTGACAGACTTGT
CGTTACTGACAACAGAAAAATTTGCGGCTTTTTATAATGATAAATTAGTTGCTGTCTATATGAAACATCCTGAAAAAGAA
GGAATCTGGAAACCCAATAAAGTATTGGTCTGA

Upstream 100 bases:

>100_bases
ATTAAAGTACTGACTATTCTGTCAGTGTTTTTGGATTGGCAACGATGACTGATAGAGACTGTAAGTGATTTTTTAGACAA
AAAATGCTATAATTTTCAGG

Downstream 100 bases:

>100_bases
TTCTAAGCACATGAAGTTAAAAGTGAGAAAAATGGAAATATTAGAATTTACTGATGAACTAAAATTTGATGAAGAACTAA
TTCTTGTCCTCGGTTATTTT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPIAVGKSTRLLEYMEAAGKIYEGQVTIGFST
ETEDADGAIVNQTPVKNNLTESEIDSAMSHFVGKIKQIPPMYSAVKINGKKLYEYARAGQTIERPAREITIKSFVRTSPI
DWNKEEGLVTFSFKVECSKGTYVRTLAVDLADSLGYAGHMSKLQRTASNGLLIKDAIKLSKIEEIKESGKLSTILYPAEY
AVSDLPRVNLTTVQFDMARVGKKFAQSDWTNEVETADSSVNKTKQQEYLLTDLSLLTTEKFAAFYNDKLVAVYMKHPEKE
GIWKPNKVLV

Sequences:

>Translated_330_residues
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPIAVGKSTRLLEYMEAAGKIYEGQVTIGFST
ETEDADGAIVNQTPVKNNLTESEIDSAMSHFVGKIKQIPPMYSAVKINGKKLYEYARAGQTIERPAREITIKSFVRTSPI
DWNKEEGLVTFSFKVECSKGTYVRTLAVDLADSLGYAGHMSKLQRTASNGLLIKDAIKLSKIEEIKESGKLSTILYPAEY
AVSDLPRVNLTTVQFDMARVGKKFAQSDWTNEVETADSSVNKTKQQEYLLTDLSLLTTEKFAAFYNDKLVAVYMKHPEKE
GIWKPNKVLV
>Mature_330_residues
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPIAVGKSTRLLEYMEAAGKIYEGQVTIGFST
ETEDADGAIVNQTPVKNNLTESEIDSAMSHFVGKIKQIPPMYSAVKINGKKLYEYARAGQTIERPAREITIKSFVRTSPI
DWNKEEGLVTFSFKVECSKGTYVRTLAVDLADSLGYAGHMSKLQRTASNGLLIKDAIKLSKIEEIKESGKLSTILYPAEY
AVSDLPRVNLTTVQFDMARVGKKFAQSDWTNEVETADSSVNKTKQQEYLLTDLSLLTTEKFAAFYNDKLVAVYMKHPEKE
GIWKPNKVLV

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=259, Percent_Identity=30.8880308880309, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI215599015, Length=304, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4503337, Length=304, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI2367200, Length=256, Percent_Identity=36.328125, Blast_Score=164, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI17553978, Length=203, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=130, Percent_Identity=43.0769230769231, Blast_Score=105, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323204, Length=206, Percent_Identity=30.5825242718447, Blast_Score=97, Evalue=4e-21,
Organism=Drosophila melanogaster, GI281364189, Length=310, Percent_Identity=25.4838709677419, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364187, Length=310, Percent_Identity=25.4838709677419, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364185, Length=310, Percent_Identity=25.4838709677419, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364183, Length=310, Percent_Identity=25.4838709677419, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI62471759, Length=310, Percent_Identity=25.4838709677419, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17975520, Length=310, Percent_Identity=25.4838709677419, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_LACLM (A2RL47)

Other databases:

- EMBL:   AM406671
- RefSeq:   YP_001032725.1
- ProteinModelPortal:   A2RL47
- SMR:   A2RL47
- STRING:   A2RL47
- GeneID:   4798154
- GenomeReviews:   AM406671_GR
- KEGG:   llm:llmg_1431
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   PRK02484
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 36561; Mature: 36561

Theoretical pI: Translated: 9.02; Mature: 9.02

Prosite motif: NA

Important sites: ACT_SITE 42-42

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPIAVGKSTRLL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHH
EYMEAAGKIYEGQVTIGFSTETEDADGAIVNQTPVKNNLTESEIDSAMSHFVGKIKQIPP
HHHHHCCCEEECEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
MYSAVKINGKKLYEYARAGQTIERPAREITIKSFVRTSPIDWNKEEGLVTFSFKVECSKG
CHHEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
TYVRTLAVDLADSLGYAGHMSKLQRTASNGLLIKDAIKLSKIEEIKESGKLSTILYPAEY
CEEEHHHHHHHHHCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCEEEEEECHHH
AVSDLPRVNLTTVQFDMARVGKKFAQSDWTNEVETADSSVNKTKQQEYLLTDLSLLTTEK
HHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FAAFYNDKLVAVYMKHPEKEGIWKPNKVLV
HHHHHCCCEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNENLNGILNVYKEAGWTSFDVVAKLRGILKTKKIGHGGTLDPSVTGVLPIAVGKSTRLL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCHHHH
EYMEAAGKIYEGQVTIGFSTETEDADGAIVNQTPVKNNLTESEIDSAMSHFVGKIKQIPP
HHHHHCCCEEECEEEEEECCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
MYSAVKINGKKLYEYARAGQTIERPAREITIKSFVRTSPIDWNKEEGLVTFSFKVECSKG
CHHEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEECCC
TYVRTLAVDLADSLGYAGHMSKLQRTASNGLLIKDAIKLSKIEEIKESGKLSTILYPAEY
CEEEHHHHHHHHHCCCHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCEEEEEECHHH
AVSDLPRVNLTTVQFDMARVGKKFAQSDWTNEVETADSSVNKTKQQEYLLTDLSLLTTEK
HHHCCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
FAAFYNDKLVAVYMKHPEKEGIWKPNKVLV
HHHHHCCCEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA