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The map label for this gene is mjaVM [H]

Identifier: 124485590

GI number: 124485590

Start: 750434

End: 751222

Strand: Reverse

Name: mjaVM [H]

Synonym: Mlab_0768

Alternate gene names: 124485590

Gene position: 751222-750434 (Counterclockwise)

Preceding gene: 124485591

Following gene: 124485579

Centisome position: 41.62

GC content: 51.33

Gene sequence:

>789_bases
ATGGACTGTGTCGAGGGAATGAGTACGTTAACGCCCGGCATGGTCGATGTGATCGTGACCTCGCCGCCATACAACATCGG
GAAAGCCTATACGACCTACGACGATACGATCCCGCGTAATACCTATCTGAAATGGATGAGGACGGTTGCCGAAGAGTCAT
ACCGCGTTCTTTCGGATCACGGTTCCTTCTATCTGAACATCGGCGGGAGCCTGAAAGACCCGTGGATCCCTATCGATGTG
GCGCAGGCGTTTCGGGAACAGGGATTTGTTCTTCAGAATATGATCCACTGGATCAAATCCATCGCACTTCCAGATGAAGG
AGTGGCAGCCGGACATTACAAACCGATAAACAGTAAACGATATCATAACGACTGTCATGAATTCATCTTTCATTTCACGA
AAAACGGCACGGTGCAGATCGACAGGCTCGCAACCGGCGTGCCGTATCAGGATAAGAGCAATGTGAACCGCTGGGGAGGG
GAAAAACGCGATCTCAGAGACCGGGGAAACACCTGGTTCATCCCGTACGAAACGATCCGGGAAAGCCGGCCGCATCCGGC
GACGTTTCCCATTCAGCTTCCCGTGATGTGCATAAAAGATCACGGATTAGATAAATGCCGGCTGGTGATGGATCCATTTA
TGGGGATTGGAAACACGGCGATTGCCGCGATCCGCCTTGGCGTCCCGTTTATCGGTTTCGAGATCGATGAGGGCTATAGA
AAGGTCGCTAACGAACGAGTTTCAGCAGAACTTCGCAGGGAATCGAATAATCAGGAAGATTGCATTTGA

Upstream 100 bases:

>100_bases
ACCGTGGATGCAAACCACCCGCTTGCAGGGGAAACCCTGACATACGAAATCACCATAGTCAAGATCGTGAAACATGACTG
AACTCAACAGGATTTACGAC

Downstream 100 bases:

>100_bases
GACTACATCATCTTAAGATTCGTTAAGATCTGTCTGTTGACGGAATTCCATTTGGTTCACAGCCTGAAAAAGAAGAGATG
GTCTGAGGCTGCATATTGCC

Product: hypothetical protein

Products: NA

Alternate protein names: M.MjaV; N-4 cytosine-specific methyltransferase MjaV [H]

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MDCVEGMSTLTPGMVDVIVTSPPYNIGKAYTTYDDTIPRNTYLKWMRTVAEESYRVLSDHGSFYLNIGGSLKDPWIPIDV
AQAFREQGFVLQNMIHWIKSIALPDEGVAAGHYKPINSKRYHNDCHEFIFHFTKNGTVQIDRLATGVPYQDKSNVNRWGG
EKRDLRDRGNTWFIPYETIRESRPHPATFPIQLPVMCIKDHGLDKCRLVMDPFMGIGNTAIAAIRLGVPFIGFEIDEGYR
KVANERVSAELRRESNNQEDCI

Sequences:

>Translated_262_residues
MDCVEGMSTLTPGMVDVIVTSPPYNIGKAYTTYDDTIPRNTYLKWMRTVAEESYRVLSDHGSFYLNIGGSLKDPWIPIDV
AQAFREQGFVLQNMIHWIKSIALPDEGVAAGHYKPINSKRYHNDCHEFIFHFTKNGTVQIDRLATGVPYQDKSNVNRWGG
EKRDLRDRGNTWFIPYETIRESRPHPATFPIQLPVMCIKDHGLDKCRLVMDPFMGIGNTAIAAIRLGVPFIGFEIDEGYR
KVANERVSAELRRESNNQEDCI
>Mature_262_residues
MDCVEGMSTLTPGMVDVIVTSPPYNIGKAYTTYDDTIPRNTYLKWMRTVAEESYRVLSDHGSFYLNIGGSLKDPWIPIDV
AQAFREQGFVLQNMIHWIKSIALPDEGVAAGHYKPINSKRYHNDCHEFIFHFTKNGTVQIDRLATGVPYQDKSNVNRWGG
EKRDLRDRGNTWFIPYETIRESRPHPATFPIQLPVMCIKDHGLDKCRLVMDPFMGIGNTAIAAIRLGVPFIGFEIDEGYR
KVANERVSAELRRESNNQEDCI

Specific function: This methylase recognizes the double-stranded sequence GTAC, causes specific methylation on C-4 on both strands, and protects the DNA from cleavage by the MjaV endonuclease (Potential) [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family. N(4) subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=261, Percent_Identity=25.2873563218391, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR017985
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.113 [H]

Molecular weight: Translated: 29888; Mature: 29888

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00093 N4_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCVEGMSTLTPGMVDVIVTSPPYNIGKAYTTYDDTIPRNTYLKWMRTVAEESYRVLSDH
CCHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GSFYLNIGGSLKDPWIPIDVAQAFREQGFVLQNMIHWIKSIALPDEGVAAGHYKPINSKR
CCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
YHNDCHEFIFHFTKNGTVQIDRLATGVPYQDKSNVNRWGGEKRDLRDRGNTWFIPYETIR
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHH
ESRPHPATFPIQLPVMCIKDHGLDKCRLVMDPFMGIGNTAIAAIRLGVPFIGFEIDEGYR
CCCCCCCCCCEEECEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECHHHH
KVANERVSAELRRESNNQEDCI
HHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MDCVEGMSTLTPGMVDVIVTSPPYNIGKAYTTYDDTIPRNTYLKWMRTVAEESYRVLSDH
CCHHHHHHHCCCCEEEEEEECCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
GSFYLNIGGSLKDPWIPIDVAQAFREQGFVLQNMIHWIKSIALPDEGVAAGHYKPINSKR
CCEEEEECCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
YHNDCHEFIFHFTKNGTVQIDRLATGVPYQDKSNVNRWGGEKRDLRDRGNTWFIPYETIR
HHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCEEEEEHHHHH
ESRPHPATFPIQLPVMCIKDHGLDKCRLVMDPFMGIGNTAIAAIRLGVPFIGFEIDEGYR
CCCCCCCCCCEEECEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECHHHH
KVANERVSAELRRESNNQEDCI
HHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]