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The map label for this gene is metG

Identifier: 124485549

GI number: 124485549

Start: 703582

End: 705603

Strand: Reverse

Name: metG

Synonym: Mlab_0726

Alternate gene names: 124485549

Gene position: 705603-703582 (Counterclockwise)

Preceding gene: 124485550

Following gene: 124485548

Centisome position: 39.09

GC content: 56.33

Gene sequence:

>2022_bases
ATGACCAATAAGCCCACTGTCGTTACGTGCGGTTTGCCCTATACAAACGGTTTTTGCCATCTCGGGCACCTCCGGACGTA
CATCCCAGGCGATTTCTACGTACGATACCTCAGGCGTCTCGGCGACGACATCGTCTTCATCTGCGGATCCGACAACCACG
GGACCCCCATCGTCGTCACGGCCGAGGCCGAAAAAACCACGCCGCGTGCCGTATCCGAAACATACCACGCCCACTTCGAC
AACGTCTTCAAATCCATGGGCATCGCATTCGACCGGTTCGGCATGACCGACGACCCGACCAACCACCACCGGACCGTCTC
CATCCTCCAGACCCTCATCGACCGCGGATATGTCTACGAACAGACCATCCAGCAAAGCTACTGCCCCAAATGCAAACGCT
TCCTCCCCGACCGGTACGTCGAAGGAACCTGCCCCTACTGCGGCAAACATGCCCGCGGGGATGAATGCGACTCGGGATGC
GGCCGGCACCTCGAACCCGGAGAGATCCTCGATCCCGTCTGCGCAACCTGCGGGACCAAAGCCGAACTCCGCGAACAGAA
GCACTACTACTTCAAGCTCAGCAGCTTTAGGGACTATCTTCTGGAGTATCTGCCGACCCTCGGCGGAACGCTCAACGCCA
AAAACTACGCCATCGGCTGGGTAGAAAACGAACTCAAAGACTGGTGCATCACCCGAATGATGGACTGGGGCGTCAAGTTC
CCGGGATCCGACGATCTCGTCTGCTATGTCTGGGTGGATGCCCCGATCGGATACATCTCGTTCACTGAAGAATGGGCGAA
AGCCACCAACAACAGCTGGCAGAAATACTGGTGCGAGGGAGATAGAGTCCACTTCATCGGGTCCGACATCATCTATCACC
ACTGTGTCTTCTGGCCGGCAATGCTTCACGGGGCCGGCTATCAGCCGCCGACCGCGGTCGTAGCGAGCGGCATGGTCACG
ATCGACGGCGAGAAGTTCTCCAAATCGAAAGGAAACGTCGTCTGGACGAAAGAGGATTACCTTGACAAGGGACTGCCGGC
GGATTATCTCCGGTATTATCTCCTCGCCTATACGAGCCACACCCGCGAACTGGACTTCTCCTGGAAAGAGTTCCAGGCAC
GGATCAATAATGAACTCGTGAACACGTTCGGGAACTTCGCGAACCGGAGCATGAGCCTGGTCAAGACCAAGTTCGGCGAT
GTGCCGCAGGTCCCGGTCGAGGCAGAGATCTTTGCCGAGATCGAACGTTCGCTCGCTGCAATTGAGGAGTCCGTCCGTGC
ATACGAGTTCAAGGCGGCCGTCGACGGCATCCTTCTTCTCGCGGCATACGGAAACAGCTATATCTCGAACGCGGCTCCCT
GGAAGCTGATCAAGGAAGATCCGCAAGCGGCCGCACAGATATTAAAGAACTGTCTCCAGATCGTGAAGGCGTGTGCTCTT
ATCATGCAGCCGGTGATGCCCGAGTCTTCCCAGAAACTTTGGGAGATGCTCGGCTATACGGACAAGATCGAGAGCCGCCC
GATCAGCGATGCGCTGGTTCCGTTCGAGAACACAACACTTGGCGATGTGAAGCCGCTGTTTGCGAGAATCGAGGACAAGC
AGCGTGAGGAGATGGAGGCCGTGCTGACAAAGCGGGCCGAGGATTCGAAGAAGAAAGCAGCAGGAAAGAATACTATGGAA
GCAGTAATAGAACCAATCTCGGAAGAGATCATAACAATAGACGATGTAGCAAAGCTCGATCTCCGCGTGGGTCGGGTCGT
GAAAGCGGAGAGAGTGCCAAAGACGACGAAACTTCTGCGTCTGCAGGTGGATATCGGAACCGAGGTCAGACAGATCGTGT
CAGGCATCGCCGATGTCTATACGCCTGAAGAGATGGTGGATAAGAAGATCATCGTGGTCGTAAACCTGAAGCCGGCAAAG
TTCCGGGGCGAGGAGAGCAACGGGATGCTGTTTGCCGCAGGCGAAGAGGCATCGCTTCTTGTTCCGCTGAAGGATGTGCC
GGAAGGAACCAAAGTCCACTGA

Upstream 100 bases:

>100_bases
TCGCACGTTTCCTTCGAAAACTTGCTCAAGCTAAGGCAACCCCTTCGTCGCCAACCCACGAAATCAGTATTTAATACCAA
TCCGCTCCAAATAGTACCGC

Downstream 100 bases:

>100_bases
TCTTTTGGCATACCTACATCACCCCCATTTTTTTTAAGTGCCGGAATAGACTGGCTTTATGTCTTCTTCCAACCATTCAT
TGATTATAGGCATCGGTTAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 673; Mature: 672

Protein sequence:

>673_residues
MTNKPTVVTCGLPYTNGFCHLGHLRTYIPGDFYVRYLRRLGDDIVFICGSDNHGTPIVVTAEAEKTTPRAVSETYHAHFD
NVFKSMGIAFDRFGMTDDPTNHHRTVSILQTLIDRGYVYEQTIQQSYCPKCKRFLPDRYVEGTCPYCGKHARGDECDSGC
GRHLEPGEILDPVCATCGTKAELREQKHYYFKLSSFRDYLLEYLPTLGGTLNAKNYAIGWVENELKDWCITRMMDWGVKF
PGSDDLVCYVWVDAPIGYISFTEEWAKATNNSWQKYWCEGDRVHFIGSDIIYHHCVFWPAMLHGAGYQPPTAVVASGMVT
IDGEKFSKSKGNVVWTKEDYLDKGLPADYLRYYLLAYTSHTRELDFSWKEFQARINNELVNTFGNFANRSMSLVKTKFGD
VPQVPVEAEIFAEIERSLAAIEESVRAYEFKAAVDGILLLAAYGNSYISNAAPWKLIKEDPQAAAQILKNCLQIVKACAL
IMQPVMPESSQKLWEMLGYTDKIESRPISDALVPFENTTLGDVKPLFARIEDKQREEMEAVLTKRAEDSKKKAAGKNTME
AVIEPISEEIITIDDVAKLDLRVGRVVKAERVPKTTKLLRLQVDIGTEVRQIVSGIADVYTPEEMVDKKIIVVVNLKPAK
FRGEESNGMLFAAGEEASLLVPLKDVPEGTKVH

Sequences:

>Translated_673_residues
MTNKPTVVTCGLPYTNGFCHLGHLRTYIPGDFYVRYLRRLGDDIVFICGSDNHGTPIVVTAEAEKTTPRAVSETYHAHFD
NVFKSMGIAFDRFGMTDDPTNHHRTVSILQTLIDRGYVYEQTIQQSYCPKCKRFLPDRYVEGTCPYCGKHARGDECDSGC
GRHLEPGEILDPVCATCGTKAELREQKHYYFKLSSFRDYLLEYLPTLGGTLNAKNYAIGWVENELKDWCITRMMDWGVKF
PGSDDLVCYVWVDAPIGYISFTEEWAKATNNSWQKYWCEGDRVHFIGSDIIYHHCVFWPAMLHGAGYQPPTAVVASGMVT
IDGEKFSKSKGNVVWTKEDYLDKGLPADYLRYYLLAYTSHTRELDFSWKEFQARINNELVNTFGNFANRSMSLVKTKFGD
VPQVPVEAEIFAEIERSLAAIEESVRAYEFKAAVDGILLLAAYGNSYISNAAPWKLIKEDPQAAAQILKNCLQIVKACAL
IMQPVMPESSQKLWEMLGYTDKIESRPISDALVPFENTTLGDVKPLFARIEDKQREEMEAVLTKRAEDSKKKAAGKNTME
AVIEPISEEIITIDDVAKLDLRVGRVVKAERVPKTTKLLRLQVDIGTEVRQIVSGIADVYTPEEMVDKKIIVVVNLKPAK
FRGEESNGMLFAAGEEASLLVPLKDVPEGTKVH
>Mature_672_residues
TNKPTVVTCGLPYTNGFCHLGHLRTYIPGDFYVRYLRRLGDDIVFICGSDNHGTPIVVTAEAEKTTPRAVSETYHAHFDN
VFKSMGIAFDRFGMTDDPTNHHRTVSILQTLIDRGYVYEQTIQQSYCPKCKRFLPDRYVEGTCPYCGKHARGDECDSGCG
RHLEPGEILDPVCATCGTKAELREQKHYYFKLSSFRDYLLEYLPTLGGTLNAKNYAIGWVENELKDWCITRMMDWGVKFP
GSDDLVCYVWVDAPIGYISFTEEWAKATNNSWQKYWCEGDRVHFIGSDIIYHHCVFWPAMLHGAGYQPPTAVVASGMVTI
DGEKFSKSKGNVVWTKEDYLDKGLPADYLRYYLLAYTSHTRELDFSWKEFQARINNELVNTFGNFANRSMSLVKTKFGDV
PQVPVEAEIFAEIERSLAAIEESVRAYEFKAAVDGILLLAAYGNSYISNAAPWKLIKEDPQAAAQILKNCLQIVKACALI
MQPVMPESSQKLWEMLGYTDKIESRPISDALVPFENTTLGDVKPLFARIEDKQREEMEAVLTKRAEDSKKKAAGKNTMEA
VIEPISEEIITIDDVAKLDLRVGRVVKAERVPKTTKLLRLQVDIGTEVRQIVSGIADVYTPEEMVDKKIIVVVNLKPAKF
RGEESNGMLFAAGEEASLLVPLKDVPEGTKVH

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=625, Percent_Identity=30.72, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI24308436, Length=565, Percent_Identity=25.4867256637168, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI4507947, Length=91, Percent_Identity=39.5604395604396, Blast_Score=67, Evalue=5e-11,
Organism=Escherichia coli, GI1788432, Length=689, Percent_Identity=33.0914368650218, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1786861, Length=198, Percent_Identity=26.2626262626263, Blast_Score=78, Evalue=2e-15,
Organism=Escherichia coli, GI1789455, Length=108, Percent_Identity=37.962962962963, Blast_Score=78, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540794, Length=595, Percent_Identity=30.0840336134454, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI212645096, Length=513, Percent_Identity=26.5107212475634, Blast_Score=168, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71992781, Length=371, Percent_Identity=27.2237196765499, Blast_Score=125, Evalue=8e-29,
Organism=Saccharomyces cerevisiae, GI6321704, Length=555, Percent_Identity=30.990990990991, Blast_Score=253, Evalue=6e-68,
Organism=Saccharomyces cerevisiae, GI6321610, Length=563, Percent_Identity=23.0905861456483, Blast_Score=120, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24655311, Length=623, Percent_Identity=31.4606741573034, Blast_Score=295, Evalue=7e-80,
Organism=Drosophila melanogaster, GI21358483, Length=524, Percent_Identity=26.9083969465649, Blast_Score=155, Evalue=1e-37,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_METLZ (A2SRE2)

Other databases:

- EMBL:   CP000559
- RefSeq:   YP_001030165.1
- ProteinModelPortal:   A2SRE2
- SMR:   A2SRE2
- STRING:   A2SRE2
- GeneID:   4795045
- GenomeReviews:   CP000559_GR
- KEGG:   mla:Mlab_0726
- eggNOG:   arNOG04371
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- PhylomeDB:   A2SRE2
- ProtClustDB:   PRK00133
- BioCyc:   MLAB410358:MLAB_0726-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75884; Mature: 75753

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKPTVVTCGLPYTNGFCHLGHLRTYIPGDFYVRYLRRLGDDIVFICGSDNHGTPIVVT
CCCCCCEEECCCCCCCCCEECCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEE
AEAEKTTPRAVSETYHAHFDNVFKSMGIAFDRFGMTDDPTNHHRTVSILQTLIDRGYVYE
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHH
QTIQQSYCPKCKRFLPDRYVEGTCPYCGKHARGDECDSGCGRHLEPGEILDPVCATCGTK
HHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
AELREQKHYYFKLSSFRDYLLEYLPTLGGTLNAKNYAIGWVENELKDWCITRMMDWGVKF
HHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCC
PGSDDLVCYVWVDAPIGYISFTEEWAKATNNSWQKYWCEGDRVHFIGSDIIYHHCVFWPA
CCCCCEEEEEEECCCCCCEEHHHHHHHHCCCCCHHEECCCCEEEEECHHHHHHHHHHHHH
MLHGAGYQPPTAVVASGMVTIDGEKFSKSKGNVVWTKEDYLDKGLPADYLRYYLLAYTSH
HHHCCCCCCCHHHHHCCEEEECCCHHCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHCC
TRELDFSWKEFQARINNELVNTFGNFANRSMSLVKTKFGDVPQVPVEAEIFAEIERSLAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
IEESVRAYEFKAAVDGILLLAAYGNSYISNAAPWKLIKEDPQAAAQILKNCLQIVKACAL
HHHHHHHHHHHHHHHHEEEEEECCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
IMQPVMPESSQKLWEMLGYTDKIESRPISDALVPFENTTLGDVKPLFARIEDKQREEMEA
HHHCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLTKRAEDSKKKAAGKNTMEAVIEPISEEIITIDDVAKLDLRVGRVVKAERVPKTTKLLR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LQVDIGTEVRQIVSGIADVYTPEEMVDKKIIVVVNLKPAKFRGEESNGMLFAAGEEASLL
EEECCCHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCCHHCCCCCCCCEEEECCCCCEEE
VPLKDVPEGTKVH
EEHHCCCCCCCCC
>Mature Secondary Structure 
TNKPTVVTCGLPYTNGFCHLGHLRTYIPGDFYVRYLRRLGDDIVFICGSDNHGTPIVVT
CCCCCEEECCCCCCCCCEECCCHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEE
AEAEKTTPRAVSETYHAHFDNVFKSMGIAFDRFGMTDDPTNHHRTVSILQTLIDRGYVYE
ECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHH
QTIQQSYCPKCKRFLPDRYVEGTCPYCGKHARGDECDSGCGRHLEPGEILDPVCATCGTK
HHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCH
AELREQKHYYFKLSSFRDYLLEYLPTLGGTLNAKNYAIGWVENELKDWCITRMMDWGVKF
HHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCCC
PGSDDLVCYVWVDAPIGYISFTEEWAKATNNSWQKYWCEGDRVHFIGSDIIYHHCVFWPA
CCCCCEEEEEEECCCCCCEEHHHHHHHHCCCCCHHEECCCCEEEEECHHHHHHHHHHHHH
MLHGAGYQPPTAVVASGMVTIDGEKFSKSKGNVVWTKEDYLDKGLPADYLRYYLLAYTSH
HHHCCCCCCCHHHHHCCEEEECCCHHCCCCCCEEEEEHHHHCCCCCHHHHHHHHHHHHCC
TRELDFSWKEFQARINNELVNTFGNFANRSMSLVKTKFGDVPQVPVEAEIFAEIERSLAA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
IEESVRAYEFKAAVDGILLLAAYGNSYISNAAPWKLIKEDPQAAAQILKNCLQIVKACAL
HHHHHHHHHHHHHHHHEEEEEECCCHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHH
IMQPVMPESSQKLWEMLGYTDKIESRPISDALVPFENTTLGDVKPLFARIEDKQREEMEA
HHHCCCCCHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
VLTKRAEDSKKKAAGKNTMEAVIEPISEEIITIDDVAKLDLRVGRVVKAERVPKTTKLLR
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEE
LQVDIGTEVRQIVSGIADVYTPEEMVDKKIIVVVNLKPAKFRGEESNGMLFAAGEEASLL
EEECCCHHHHHHHHHHHHHCCCHHHCCCEEEEEEECCCHHCCCCCCCCEEEECCCCCEEE
VPLKDVPEGTKVH
EEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA