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The map label for this gene is murD [H]

Identifier: 124265653

GI number: 124265653

Start: 499177

End: 500901

Strand: Direct

Name: murD [H]

Synonym: Mpe_A0460

Alternate gene names: 124265653

Gene position: 499177-500901 (Clockwise)

Preceding gene: 124265652

Following gene: 124265654

Centisome position: 12.34

GC content: 73.8

Gene sequence:

>1725_bases
ATGAAACCTCTGGACCACCAGACCGTGCTCATGCTGGGCCTCGGCGACTCGGGCCTCGCGATGGCGCAGTGGTGCGCGCG
CCAGGGCGCCGCGGTGCGCGTGGCCGACAGCCGCGAGGCGCCGCCGCAGGCCGCAGCGTTGGCCCAGGCGCTGCCGGGCG
CGACGCTGCACCACGGTTTCGACGCTGGCCTGCTGGACGGCGTGCAGCGCGTGCTGAAGAGCCCGGGCCTGTCGCCGCTG
GACCCGGTGCTGGCGCCGCTGCTGGCGCGCGCGGCCGAGCTTGGCGTACCCGTCGAGGGCGAGCTCGACCTGTTCGCGCA
GGCCCTGGCCGGGCTGAAGGCCGAACGCGGCTACGCACCCAAGGTGATCGCCATCACCGGCACCAACGGCAAGACCACCA
CCACGGTGATGTGCGGCCAGCTGATCGAACGCAGCGGATTGCGCGTGGCGGTGGCCGGCAACGTGGGCCCGACGATGCTG
GGCACGCTGGCGGCGGCGCTGGAGCAGGAAGGTGAACCGGAATGCCCCTCACCCCAGCCCTCTCCCGCCCCAGGGGGAGA
GGGAGACGAACCGCCGGCTCCCACTCCGATCTCAGCGGGAGAGAGCGGGGGTGAGGGCCTTCCCGTGTCCGAACCTGCCG
ACGACGACGAAGCCCCGCTGCAGCTCGCCCCGCCGCCGCCCGCGGCGCCGGTGTTCGGGCACCTTCCCGAGGCCTGGGTG
CTGGAGCTGTCGAGCTTCCAGCTCGACGGCGTGCGCGGCTTCGTGCCGGACGCCGCGGTGGTGCTGAACATCACGCAGGA
CCACCTCGACTGGCACGGCTCGATGCCGGCCTATGCCGCGGCGAAGGCGCGCATCTACGGCCCGGCCGACGCCTGGGGCT
GCACGATGGTGGTCAACCGCGACGACGTGCAGGTCGAGGCCATGGTCCCGGCGCCATTGCCGCAGAAAGGCCGCTTCGCG
AAGCCGCTGGCGCGCAAGGTGGTCCGCTTCGGGCTCGACGCGCCGCAGCGCCCCGGCGACTACGGGCTGGTGGTCGAGAA
CGGCATGGCCTGGCTGGTGCGCGCGCTGGAGGCCGACGAGACGATCAAGCGCCGCAAGGGCGACACCGAGGACGAGGAGA
TCCACCTGCAGCGCCTGATGCCGGCCGACGCGCTGCGCATCCGCGGCCGCCACAACGCCGCCAACGCGCTGGCCGCGCTG
GCGCTGGCCACCGCCATCGGCCGGCCGCTGGCGCCGATGCTGCACGGCCTGCGCGAGTACGGCGGCGAGCCGCACCGCGT
GGCTTTCGTCGCCACGGTCGGGGGCGTCGAGGCCTACGACGACAGCAAGGGCACCAACGTCGGCGCCACGGTGGCCGCGC
TCGAGGGTCTGGGCGCCGACAAGACGCCGGCCAAGCTGGTGGTGATCCTCGGCGGCGACGGCAAGGGCCAGGACTTCACG
CCGCTGGTCGCGCCGGTGGCGCGCCATGTGCGCGCGGTGGCGCTGATCGGCCGCGATGCGCCGGCGATCGAGGCGGTGCT
CGCGGCCGGCAGTGTGCCGCTGGCGCGGCACGACACGCTGCAGACCGCCACCCGCTGGTGCTTCGAGCAGGCGCGCGCCG
GCGATGCCGTGCTGCTGAGCCCGGCCTGCGCCAGCCTGGACATGTTCCGCAACTACGCGCAGCGCGCCGAGGTGTTCTGC
GACACCGTGCGCGAGCTGGCGTGCGAGCACGGGGAGACGGCATGA

Upstream 100 bases:

>100_bases
GGCTGGAAGGAGACGCAGGTCGTCGTGCGCTTCTGGATCATCACCATGCTGCTGTGCCTGGTCGGGCTGTCCAGCCTGAA
GCTGCGCTGAACCGACGCCC

Downstream 100 bases:

>100_bases
GCCGCGCCGCGTCGTCCGCCGCCGTCGGCGCCCGCCCGGGGCTGGCCGCGCTGAAGGAGCGCGCGGCGCGCTGGCTGGGC
CGCGGTGCGGGCAGCGAGAC

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MKPLDHQTVLMLGLGDSGLAMAQWCARQGAAVRVADSREAPPQAAALAQALPGATLHHGFDAGLLDGVQRVLKSPGLSPL
DPVLAPLLARAAELGVPVEGELDLFAQALAGLKAERGYAPKVIAITGTNGKTTTTVMCGQLIERSGLRVAVAGNVGPTML
GTLAAALEQEGEPECPSPQPSPAPGGEGDEPPAPTPISAGESGGEGLPVSEPADDDEAPLQLAPPPPAAPVFGHLPEAWV
LELSSFQLDGVRGFVPDAAVVLNITQDHLDWHGSMPAYAAAKARIYGPADAWGCTMVVNRDDVQVEAMVPAPLPQKGRFA
KPLARKVVRFGLDAPQRPGDYGLVVENGMAWLVRALEADETIKRRKGDTEDEEIHLQRLMPADALRIRGRHNAANALAAL
ALATAIGRPLAPMLHGLREYGGEPHRVAFVATVGGVEAYDDSKGTNVGATVAALEGLGADKTPAKLVVILGGDGKGQDFT
PLVAPVARHVRAVALIGRDAPAIEAVLAAGSVPLARHDTLQTATRWCFEQARAGDAVLLSPACASLDMFRNYAQRAEVFC
DTVRELACEHGETA

Sequences:

>Translated_574_residues
MKPLDHQTVLMLGLGDSGLAMAQWCARQGAAVRVADSREAPPQAAALAQALPGATLHHGFDAGLLDGVQRVLKSPGLSPL
DPVLAPLLARAAELGVPVEGELDLFAQALAGLKAERGYAPKVIAITGTNGKTTTTVMCGQLIERSGLRVAVAGNVGPTML
GTLAAALEQEGEPECPSPQPSPAPGGEGDEPPAPTPISAGESGGEGLPVSEPADDDEAPLQLAPPPPAAPVFGHLPEAWV
LELSSFQLDGVRGFVPDAAVVLNITQDHLDWHGSMPAYAAAKARIYGPADAWGCTMVVNRDDVQVEAMVPAPLPQKGRFA
KPLARKVVRFGLDAPQRPGDYGLVVENGMAWLVRALEADETIKRRKGDTEDEEIHLQRLMPADALRIRGRHNAANALAAL
ALATAIGRPLAPMLHGLREYGGEPHRVAFVATVGGVEAYDDSKGTNVGATVAALEGLGADKTPAKLVVILGGDGKGQDFT
PLVAPVARHVRAVALIGRDAPAIEAVLAAGSVPLARHDTLQTATRWCFEQARAGDAVLLSPACASLDMFRNYAQRAEVFC
DTVRELACEHGETA
>Mature_574_residues
MKPLDHQTVLMLGLGDSGLAMAQWCARQGAAVRVADSREAPPQAAALAQALPGATLHHGFDAGLLDGVQRVLKSPGLSPL
DPVLAPLLARAAELGVPVEGELDLFAQALAGLKAERGYAPKVIAITGTNGKTTTTVMCGQLIERSGLRVAVAGNVGPTML
GTLAAALEQEGEPECPSPQPSPAPGGEGDEPPAPTPISAGESGGEGLPVSEPADDDEAPLQLAPPPPAAPVFGHLPEAWV
LELSSFQLDGVRGFVPDAAVVLNITQDHLDWHGSMPAYAAAKARIYGPADAWGCTMVVNRDDVQVEAMVPAPLPQKGRFA
KPLARKVVRFGLDAPQRPGDYGLVVENGMAWLVRALEADETIKRRKGDTEDEEIHLQRLMPADALRIRGRHNAANALAAL
ALATAIGRPLAPMLHGLREYGGEPHRVAFVATVGGVEAYDDSKGTNVGATVAALEGLGADKTPAKLVVILGGDGKGQDFT
PLVAPVARHVRAVALIGRDAPAIEAVLAAGSVPLARHDTLQTATRWCFEQARAGDAVLLSPACASLDMFRNYAQRAEVFC
DTVRELACEHGETA

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=562, Percent_Identity=31.4946619217082, Blast_Score=186, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 59784; Mature: 59784

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLDHQTVLMLGLGDSGLAMAQWCARQGAAVRVADSREAPPQAAALAQALPGATLHHGF
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCHHHCCC
DAGLLDGVQRVLKSPGLSPLDPVLAPLLARAAELGVPVEGELDLFAQALAGLKAERGYAP
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHCCCCCC
KVIAITGTNGKTTTTVMCGQLIERSGLRVAVAGNVGPTMLGTLAAALEQEGEPECPSPQP
EEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCC
SPAPGGEGDEPPAPTPISAGESGGEGLPVSEPADDDEAPLQLAPPPPAAPVFGHLPEAWV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCHHHH
LELSSFQLDGVRGFVPDAAVVLNITQDHLDWHGSMPAYAAAKARIYGPADAWGCTMVVNR
HHHHCCEECCCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHCEEEECCCCCCCEEEEEEC
DDVQVEAMVPAPLPQKGRFAKPLARKVVRFGLDAPQRPGDYGLVVENGMAWLVRALEADE
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHH
TIKRRKGDTEDEEIHLQRLMPADALRIRGRHNAANALAALALATAIGRPLAPMLHGLREY
HHHHHCCCCCHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GGEPHRVAFVATVGGVEAYDDSKGTNVGATVAALEGLGADKTPAKLVVILGGDGKGQDFT
CCCCCEEEEEEEECCCEECCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCC
PLVAPVARHVRAVALIGRDAPAIEAVLAAGSVPLARHDTLQTATRWCFEQARAGDAVLLS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
PACASLDMFRNYAQRAEVFCDTVRELACEHGETA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPLDHQTVLMLGLGDSGLAMAQWCARQGAAVRVADSREAPPQAAALAQALPGATLHHGF
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCCCHHHCCC
DAGLLDGVQRVLKSPGLSPLDPVLAPLLARAAELGVPVEGELDLFAQALAGLKAERGYAP
CHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHCCCCCC
KVIAITGTNGKTTTTVMCGQLIERSGLRVAVAGNVGPTMLGTLAAALEQEGEPECPSPQP
EEEEEECCCCCEEHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCC
SPAPGGEGDEPPAPTPISAGESGGEGLPVSEPADDDEAPLQLAPPPPAAPVFGHLPEAWV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCHHCCCCHHHH
LELSSFQLDGVRGFVPDAAVVLNITQDHLDWHGSMPAYAAAKARIYGPADAWGCTMVVNR
HHHHCCEECCCCCCCCCCEEEEEEEHHHHCCCCCCCHHHHHCEEEECCCCCCCEEEEEEC
DDVQVEAMVPAPLPQKGRFAKPLARKVVRFGLDAPQRPGDYGLVVENGMAWLVRALEADE
CCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHHHH
TIKRRKGDTEDEEIHLQRLMPADALRIRGRHNAANALAALALATAIGRPLAPMLHGLREY
HHHHHCCCCCHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GGEPHRVAFVATVGGVEAYDDSKGTNVGATVAALEGLGADKTPAKLVVILGGDGKGQDFT
CCCCCEEEEEEEECCCEECCCCCCCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCC
PLVAPVARHVRAVALIGRDAPAIEAVLAAGSVPLARHDTLQTATRWCFEQARAGDAVLLS
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEC
PACASLDMFRNYAQRAEVFCDTVRELACEHGETA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA