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The map label for this gene is mraW

Identifier: 124265647

GI number: 124265647

Start: 491966

End: 492922

Strand: Direct

Name: mraW

Synonym: Mpe_A0454

Alternate gene names: 124265647

Gene position: 491966-492922 (Clockwise)

Preceding gene: 124265646

Following gene: 124265648

Centisome position: 12.16

GC content: 72.41

Gene sequence:

>957_bases
ATGAACCAGGGAGTCGGGCCTTGGCAGCACACCACCGTCCTGCTGCACGAGGCGGTCGATGCGCTCGTGCACTCGCCGGA
CGGCTACTACGTCGACGGCACCTACGGCCGCGGCGGGCACTCGCGGCTGATCCTGTCGAAGCTGTCCGCGGCGGGCCGCC
TGCTGGCGATCGACCGCGACCCCGACGCGGTGGCGCACGCCACCTCGGGCGCGGCGCGGGTCACGGACCCGCGCTTCCAC
ATCGAGCACGCGCCCTACAGCGAGCTGCCGGCGCTGCTGGCGGCGCGTGGCGTGTCACGCATTGACGGCCTGCTGCTGGA
CATCGGTGTGAGCTCGCCGCAGATCGACAACCCGGAGCGCGGCTTCAGCTTCCGCGCCAGCGGTCCGCTGGACATGCGCA
TGGACCCGACGCGTGGAGAGAGCGCGGCCGACTACCTGGCGCGCGCCGATGAACGCGAGATCGCGGCGGTGATCCGCGAC
TACGGCGAGGAACGCTTCGCGACCGCGATCGCCCGTGCGCTGGTGGCGCGCCGCCTGGCCGGCCGTCCGGTACGCACCAC
CGAGGAACTCGCGGAGCTGGTGGCGCAGGCGGTCAAGACCCGCGAGCCTGGGCAGAACCCGGCCACCCGCACCTTCCAGG
CGCTGCGCATCTTCGTCAACGCCGAGCTGACGCAGCTCGAACAAGGCCTGGCGGCGGCACTCCAATGCCTGAAGCCGGGC
GGCCGGCTGGTCGTGATCAGCTTCCACTCGCTCGAGGACCGCATCGTCAAGACCTTCATCGCGAGTCACAGCAAGGCGGT
GGTCGACCGCCGGGCGCCGTTCGCGCCGCCGGCGCCGATGAGGCTGCGCGCGCTGGCGCGCCTGAAGCCCGGCGCCGACG
AGGTGGCAGCCAATCCGCGCGCGCGCTCGGCCATCCTGCGCGTGGCCGAACGGACCGACGTGGGGATCGCCGCATGA

Upstream 100 bases:

>100_bases
ATTTTGAACTCTGGGACGTCCAGCGCTACCAGGCCCACGAGGCCGAGGTGATGCAGCAGGGGCTGCCCGAGAGCCTGGGC
GACTTCTCGTTCTGACGGCG

Downstream 100 bases:

>100_bases
CACGCCTCAACATCGTGGTGCTGATCGCGCTGGTGTGCAGCTCGCTCTACCTCGTCAAGGTGTCCTACGAGTCGCGCCGT
CTGTTCGCCGAACTCGATCG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNQGVGPWQHTTVLLHEAVDALVHSPDGYYVDGTYGRGGHSRLILSKLSAAGRLLAIDRDPDAVAHATSGAARVTDPRFH
IEHAPYSELPALLAARGVSRIDGLLLDIGVSSPQIDNPERGFSFRASGPLDMRMDPTRGESAADYLARADEREIAAVIRD
YGEERFATAIARALVARRLAGRPVRTTEELAELVAQAVKTREPGQNPATRTFQALRIFVNAELTQLEQGLAAALQCLKPG
GRLVVISFHSLEDRIVKTFIASHSKAVVDRRAPFAPPAPMRLRALARLKPGADEVAANPRARSAILRVAERTDVGIAA

Sequences:

>Translated_318_residues
MNQGVGPWQHTTVLLHEAVDALVHSPDGYYVDGTYGRGGHSRLILSKLSAAGRLLAIDRDPDAVAHATSGAARVTDPRFH
IEHAPYSELPALLAARGVSRIDGLLLDIGVSSPQIDNPERGFSFRASGPLDMRMDPTRGESAADYLARADEREIAAVIRD
YGEERFATAIARALVARRLAGRPVRTTEELAELVAQAVKTREPGQNPATRTFQALRIFVNAELTQLEQGLAAALQCLKPG
GRLVVISFHSLEDRIVKTFIASHSKAVVDRRAPFAPPAPMRLRALARLKPGADEVAANPRARSAILRVAERTDVGIAA
>Mature_318_residues
MNQGVGPWQHTTVLLHEAVDALVHSPDGYYVDGTYGRGGHSRLILSKLSAAGRLLAIDRDPDAVAHATSGAARVTDPRFH
IEHAPYSELPALLAARGVSRIDGLLLDIGVSSPQIDNPERGFSFRASGPLDMRMDPTRGESAADYLARADEREIAAVIRD
YGEERFATAIARALVARRLAGRPVRTTEELAELVAQAVKTREPGQNPATRTFQALRIFVNAELTQLEQGLAAALQCLKPG
GRLVVISFHSLEDRIVKTFIASHSKAVVDRRAPFAPPAPMRLRALARLKPGADEVAANPRARSAILRVAERTDVGIAA

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=340, Percent_Identity=39.7058823529412, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI165377202, Length=199, Percent_Identity=39.6984924623116, Blast_Score=123, Evalue=3e-28,
Organism=Escherichia coli, GI1786270, Length=319, Percent_Identity=56.4263322884012, Blast_Score=338, Evalue=2e-94,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=39.4029850746269, Blast_Score=192, Evalue=2e-49,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=39.4029850746269, Blast_Score=192, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_METPP (A2SCX7)

Other databases:

- EMBL:   CP000555
- RefSeq:   YP_001019651.1
- ProteinModelPortal:   A2SCX7
- SMR:   A2SCX7
- STRING:   A2SCX7
- GeneID:   4784173
- GenomeReviews:   CP000555_GR
- KEGG:   mpt:Mpe_A0454
- NMPDR:   fig|279263.3.peg.2697
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   A2SCX7
- ProtClustDB:   PRK00050
- BioCyc:   MPET420662:MPE_A0454-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34316; Mature: 34316

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: BINDING 58-58 BINDING 86-86 BINDING 107-107 BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQGVGPWQHTTVLLHEAVDALVHSPDGYYVDGTYGRGGHSRLILSKLSAAGRLLAIDRD
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEEECC
PDAVAHATSGAARVTDPRFHIEHAPYSELPALLAARGVSRIDGLLLDIGVSSPQIDNPER
CCHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
GFSFRASGPLDMRMDPTRGESAADYLARADEREIAAVIRDYGEERFATAIARALVARRLA
CCEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
GRPVRTTEELAELVAQAVKTREPGQNPATRTFQALRIFVNAELTQLEQGLAAALQCLKPG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRLVVISFHSLEDRIVKTFIASHSKAVVDRRAPFAPPAPMRLRALARLKPGADEVAANPR
CCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCH
ARSAILRVAERTDVGIAA
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNQGVGPWQHTTVLLHEAVDALVHSPDGYYVDGTYGRGGHSRLILSKLSAAGRLLAIDRD
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHCCCEEEEEECC
PDAVAHATSGAARVTDPRFHIEHAPYSELPALLAARGVSRIDGLLLDIGVSSPQIDNPER
CCHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCC
GFSFRASGPLDMRMDPTRGESAADYLARADEREIAAVIRDYGEERFATAIARALVARRLA
CCEEECCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
GRPVRTTEELAELVAQAVKTREPGQNPATRTFQALRIFVNAELTQLEQGLAAALQCLKPG
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GRLVVISFHSLEDRIVKTFIASHSKAVVDRRAPFAPPAPMRLRALARLKPGADEVAANPR
CCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCHHHHHCCCH
ARSAILRVAERTDVGIAA
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA