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The map label for this gene is insH10 [H]

Identifier: 124265575

GI number: 124265575

Start: 416207

End: 417166

Strand: Direct

Name: insH10 [H]

Synonym: Mpe_A0382

Alternate gene names: 124265575

Gene position: 416207-417166 (Clockwise)

Preceding gene: 124265573

Following gene: 124265576

Centisome position: 10.29

GC content: 65.94

Gene sequence:

>960_bases
ATGAAGCAGCTGGGTTTGGGCCTGAACCTGTCGACCAAGAAGACGCGCAAGCGCGAGTTCCTGGAGGACATGGAGCGCGT
GGTGCCGTGGGCGGTGCTGGTGCAGATCGTGGCGCCGCACTACCCCAAGGCCAAGACCGGCCGCCCGCCCTTCGGCATCG
AGACCATGCTGCGCATCCACTACCTGCAGCAGTGGTTCGGCCTGAGCGACCCGGCGATGGAAGAGGCGCTGCACGACGTG
CCGCTGTACCGGGAGTTCGCCAAGCTCGACAGCGTGACGGCACGGCTGCCTGATGAGACCACCATCCTGAGGTTCCGCCA
CCTGCTGGAGCGCCACAACCTGGCCGTGGACATGCTGCGCGTGGTCAACGATCTGCTTCAGCACAAGGGCCTGATGATGC
GCACGGGCACGGCGGTGGACGCCACGCTGATCTCGGCGCCGAGTTCGACCAAGAACGCCGAAGGTGAGCGCGACCCGGAG
ATGAAGCAGACCCGCAAGGGCAACAACTGGTACTTCGGCATGAAGGCCCACATCGGCGTGGACGCGCAGTCGGGTCTGGT
GCACACCGTGGCCACCACGGGGGCCAACGTCAACGACCTGAACATGGCCGGCGCGCTGCTGCACGGCGATGAGGAAGCTG
CCTTCGGTGACGCGGGCTACCAGGGCGTGCACAAGCGGCCCGAGGCCAAGGGGCCGACCTGGCACGTGGCAATGCGCCCG
GGCCTGCGCAGGAAGCTCAACCCCTTCATCGAGCCCGACTTCATCGCCGAGCGCATCGAGAAGATGAAGGCCAGCATCCG
GGCCAAGGTCGAGCATCCGTTCCGGGTGATCAAGCGCCAGTTCGGGTTCACCAAGGTGCGCTACCGGGGGCTGAAGAAGA
ACACCGCGCAGATCGTCACGCTGTTCGCGCTGTCGAACCTGTGGATGGCGCGACGGCAGTTGATCGGAGCGCGGGGATGA

Upstream 100 bases:

>100_bases
TCCCGGCGCTCGCGCCTCAGCTCAAACGTTAGGGAACGTCTGCAAAACTCGTCCGAATCTTGCATGAGTTGAAGCGACGA
CGCATCGGAGCACGACGACC

Downstream 100 bases:

>100_bases
GTGCGTCCGCGATACCCGCAGCGGGCTCGCGAGGTGCCAAAAGCACCTGCGAAACGGCACGACCGAGCCGCAGAACAGAC
CGGATCGAGGGCTTCGGCGC

Product: ISRSO18-transposase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKQLGLGLNLSTKKTRKREFLEDMERVVPWAVLVQIVAPHYPKAKTGRPPFGIETMLRIHYLQQWFGLSDPAMEEALHDV
PLYREFAKLDSVTARLPDETTILRFRHLLERHNLAVDMLRVVNDLLQHKGLMMRTGTAVDATLISAPSSTKNAEGERDPE
MKQTRKGNNWYFGMKAHIGVDAQSGLVHTVATTGANVNDLNMAGALLHGDEEAAFGDAGYQGVHKRPEAKGPTWHVAMRP
GLRRKLNPFIEPDFIAERIEKMKASIRAKVEHPFRVIKRQFGFTKVRYRGLKKNTAQIVTLFALSNLWMARRQLIGARG

Sequences:

>Translated_319_residues
MKQLGLGLNLSTKKTRKREFLEDMERVVPWAVLVQIVAPHYPKAKTGRPPFGIETMLRIHYLQQWFGLSDPAMEEALHDV
PLYREFAKLDSVTARLPDETTILRFRHLLERHNLAVDMLRVVNDLLQHKGLMMRTGTAVDATLISAPSSTKNAEGERDPE
MKQTRKGNNWYFGMKAHIGVDAQSGLVHTVATTGANVNDLNMAGALLHGDEEAAFGDAGYQGVHKRPEAKGPTWHVAMRP
GLRRKLNPFIEPDFIAERIEKMKASIRAKVEHPFRVIKRQFGFTKVRYRGLKKNTAQIVTLFALSNLWMARRQLIGARG
>Mature_319_residues
MKQLGLGLNLSTKKTRKREFLEDMERVVPWAVLVQIVAPHYPKAKTGRPPFGIETMLRIHYLQQWFGLSDPAMEEALHDV
PLYREFAKLDSVTARLPDETTILRFRHLLERHNLAVDMLRVVNDLLQHKGLMMRTGTAVDATLISAPSSTKNAEGERDPE
MKQTRKGNNWYFGMKAHIGVDAQSGLVHTVATTGANVNDLNMAGALLHGDEEAAFGDAGYQGVHKRPEAKGPTWHVAMRP
GLRRKLNPFIEPDFIAERIEKMKASIRAKVEHPFRVIKRQFGFTKVRYRGLKKNTAQIVTLFALSNLWMARRQLIGARG

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: COG3039

COG function: function code L; Transposase and inactivated derivatives, IS5 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1788303, Length=300, Percent_Identity=55.6666666666667, Blast_Score=338, Evalue=4e-94,
Organism=Escherichia coli, GI1789921, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1789612, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1789355, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1788518, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1788342, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1787592, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1786877, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1786764, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1786453, Length=300, Percent_Identity=55.3333333333333, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1787634, Length=303, Percent_Identity=54.7854785478548, Blast_Score=332, Evalue=2e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 36121; Mature: 36121

Theoretical pI: Translated: 10.70; Mature: 10.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLGLGLNLSTKKTRKREFLEDMERVVPWAVLVQIVAPHYPKAKTGRPPFGIETMLRIH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
YLQQWFGLSDPAMEEALHDVPLYREFAKLDSVTARLPDETTILRFRHLLERHNLAVDMLR
HHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VVNDLLQHKGLMMRTGTAVDATLISAPSSTKNAEGERDPEMKQTRKGNNWYFGMKAHIGV
HHHHHHHHCCCEEECCCCHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEECC
DAQSGLVHTVATTGANVNDLNMAGALLHGDEEAAFGDAGYQGVHKRPEAKGPTWHVAMRP
CCCCCEEEEEECCCCCCCCHHHHHHEEECCCHHCCCCCCCCCCCCCCCCCCCEEEEEECC
GLRRKLNPFIEPDFIAERIEKMKASIRAKVEHPFRVIKRQFGFTKVRYRGLKKNTAQIVT
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHH
LFALSNLWMARRQLIGARG
HHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKQLGLGLNLSTKKTRKREFLEDMERVVPWAVLVQIVAPHYPKAKTGRPPFGIETMLRIH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHH
YLQQWFGLSDPAMEEALHDVPLYREFAKLDSVTARLPDETTILRFRHLLERHNLAVDMLR
HHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
VVNDLLQHKGLMMRTGTAVDATLISAPSSTKNAEGERDPEMKQTRKGNNWYFGMKAHIGV
HHHHHHHHCCCEEECCCCHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCEEEEEEEEECC
DAQSGLVHTVATTGANVNDLNMAGALLHGDEEAAFGDAGYQGVHKRPEAKGPTWHVAMRP
CCCCCEEEEEECCCCCCCCHHHHHHEEECCCHHCCCCCCCCCCCCCCCCCCCEEEEEECC
GLRRKLNPFIEPDFIAERIEKMKASIRAKVEHPFRVIKRQFGFTKVRYRGLKKNTAQIVT
CHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHH
LFALSNLWMARRQLIGARG
HHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]