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The map label for this gene is pykF [H]

Identifier: 124265483

GI number: 124265483

Start: 314322

End: 315758

Strand: Direct

Name: pykF [H]

Synonym: Mpe_A0290

Alternate gene names: 124265483

Gene position: 314322-315758 (Clockwise)

Preceding gene: 124265482

Following gene: 124265484

Centisome position: 7.77

GC content: 67.43

Gene sequence:

>1437_bases
ATGACCATGCACCGCGCCACCAAGATCGTCGCCACCCTCGGCCCCGCCTCGTCCACGCCCGACGTGCTTGAACGCATGAT
CACCGCCGGCGTCGACGTCGTCCGGCTGAACTTCTCGCACGGCAGCGCGCAGGACCACATCGCACGTGCCGCCCTGGTGC
GCGAGGTGGCGCAGCGCGCCGGCAAGGAGGTCGCGATCATGGCCGATCTGCAAGGGCCGAAGATCCGTGTCGGCAAGTTC
GCGCAGGGCAAGGTGATGCTGGAGCCTGGCGCGGCGTTCGTACTGGACGCCGCCCGCAGCGAGCCGGGCGACCTGCAGGC
GGTCGGCCTGGACTACAAGTCGCTGCCGCGCGACGTGAAGCCGGGCGACACGCTGCTGCTCAACGACGGACTGATCGTGC
TGACCGTCGACAAGGTGATCGGCGAGGCGGTGCACACCGTCGTGAAGATCGGCGGCGAGCTGTCCAACAACAAGGGCATC
AACAAGCAGGGCGGCGGCCTCACGGCGCCGGCGCTGACCGCGAAGGACATGGAGGACATCAAGACCGCGATGAGCTTCCA
GTGCGAGTACCTCGCGGTCAGCTTCCCCAAGAGCGCGACCGACATGGAGATGGCCCGGCAGCTGGCCAACGTGGCCGGTG
AGCCGTGGCGTCACAAGCCGGCGCTGATCGCGAAGATCGAGCGCAGCGAGGCCATCCCCGTGCTCGAGCAGATCCTCAAG
GCTAGCGACGGCATCATGGTGGCGCGCGGTGACCTCGCGGTCGAGGTCGGCAACGCCGCGGTGCCGGCGTTGCAGAAGAA
GATGATCAAGCTCGCCCGGGAGCTCGACAAGGTGGTGATCACCGCGACCCAGATGATGGAGTCGATGATCGTCAACCCGG
TGCCGACGCGCGCCGAGGTGAGCGACGTCGCGAATGCCGTGCTCGACGGCACCGATGCGGTGATGCTCAGCGCCGAGACA
GCGGCCGGCAAGTACCCGGTCGAGACCATCGAACAGATGGCCGCCATCGCACTGGCAGCCGAACGGGCCGAGGACGTGGC
GCTGGACGCCGACTTCACCAACAAGCGCTTCGGCCGCATCGACCAGAGCATCGCGATGGGTGCGCTGTTCACCGCCCACC
ACCTGGGCTGCAAGGCGATCGTCGCGCTGACGGAATCGGGCTCGACGGCGCTGTGGATGAGCCGCCACAAGATCCACGTG
CCGATCTTCGCGCTGACTTCGCAGCAGACCAGCCAGCGCAAGATGACGCTGTATCGCAACGTGCGTCCGCTGCTGATGCC
GTCGTACACCGACCGCGACGAGGCCCTCGCGAAGGCGGAGGCGCTGCTGGTGGACGGTGGCGTGCTCAAGCCGGGAGACA
CCTACGCCATCACGTGCGGCGAGCCGATGGGCTACCCGGGCGGTACCAACATGCTCAAGGTTTGCCGAGTCGCCTGA

Upstream 100 bases:

>100_bases
CCTGCCGGCCTTCGAGATCCTCGAGCAGCGGGCCGCCGGCTGAGCGGCATGACGACACGGGGCGCTGCGGCGCCCCTCGC
TTCATCGGAATCTTCTTCAC

Downstream 100 bases:

>100_bases
GCAAGGGGGTCACGGACGGTCTCCGGCGCCGATCGGACGCCTAAAATCAGGGTTTCGCGGGCTCGCCGTCCGGCCTGCCG
CTCCTTTCGCTGTCGACTTT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MTMHRATKIVATLGPASSTPDVLERMITAGVDVVRLNFSHGSAQDHIARAALVREVAQRAGKEVAIMADLQGPKIRVGKF
AQGKVMLEPGAAFVLDAARSEPGDLQAVGLDYKSLPRDVKPGDTLLLNDGLIVLTVDKVIGEAVHTVVKIGGELSNNKGI
NKQGGGLTAPALTAKDMEDIKTAMSFQCEYLAVSFPKSATDMEMARQLANVAGEPWRHKPALIAKIERSEAIPVLEQILK
ASDGIMVARGDLAVEVGNAAVPALQKKMIKLARELDKVVITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAET
AAGKYPVETIEQMAAIALAAERAEDVALDADFTNKRFGRIDQSIAMGALFTAHHLGCKAIVALTESGSTALWMSRHKIHV
PIFALTSQQTSQRKMTLYRNVRPLLMPSYTDRDEALAKAEALLVDGGVLKPGDTYAITCGEPMGYPGGTNMLKVCRVA

Sequences:

>Translated_478_residues
MTMHRATKIVATLGPASSTPDVLERMITAGVDVVRLNFSHGSAQDHIARAALVREVAQRAGKEVAIMADLQGPKIRVGKF
AQGKVMLEPGAAFVLDAARSEPGDLQAVGLDYKSLPRDVKPGDTLLLNDGLIVLTVDKVIGEAVHTVVKIGGELSNNKGI
NKQGGGLTAPALTAKDMEDIKTAMSFQCEYLAVSFPKSATDMEMARQLANVAGEPWRHKPALIAKIERSEAIPVLEQILK
ASDGIMVARGDLAVEVGNAAVPALQKKMIKLARELDKVVITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAET
AAGKYPVETIEQMAAIALAAERAEDVALDADFTNKRFGRIDQSIAMGALFTAHHLGCKAIVALTESGSTALWMSRHKIHV
PIFALTSQQTSQRKMTLYRNVRPLLMPSYTDRDEALAKAEALLVDGGVLKPGDTYAITCGEPMGYPGGTNMLKVCRVA
>Mature_477_residues
TMHRATKIVATLGPASSTPDVLERMITAGVDVVRLNFSHGSAQDHIARAALVREVAQRAGKEVAIMADLQGPKIRVGKFA
QGKVMLEPGAAFVLDAARSEPGDLQAVGLDYKSLPRDVKPGDTLLLNDGLIVLTVDKVIGEAVHTVVKIGGELSNNKGIN
KQGGGLTAPALTAKDMEDIKTAMSFQCEYLAVSFPKSATDMEMARQLANVAGEPWRHKPALIAKIERSEAIPVLEQILKA
SDGIMVARGDLAVEVGNAAVPALQKKMIKLARELDKVVITATQMMESMIVNPVPTRAEVSDVANAVLDGTDAVMLSAETA
AGKYPVETIEQMAAIALAAERAEDVALDADFTNKRFGRIDQSIAMGALFTAHHLGCKAIVALTESGSTALWMSRHKIHVP
IFALTSQQTSQRKMTLYRNVRPLLMPSYTDRDEALAKAEALLVDGGVLKPGDTYAITCGEPMGYPGGTNMLKVCRVA

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=499, Percent_Identity=36.0721442885772, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI33286420, Length=499, Percent_Identity=36.0721442885772, Blast_Score=262, Evalue=6e-70,
Organism=Homo sapiens, GI32967597, Length=498, Percent_Identity=36.9477911646586, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI10835121, Length=498, Percent_Identity=36.9477911646586, Blast_Score=260, Evalue=3e-69,
Organism=Homo sapiens, GI33286418, Length=496, Percent_Identity=36.2903225806452, Blast_Score=259, Evalue=5e-69,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=35.3951890034364, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=35.3951890034364, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=32.6359832635983, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=32.6359832635983, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.651376146789, Blast_Score=102, Evalue=6e-22,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=51.255230125523, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=38.4288747346072, Blast_Score=294, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI17544584, Length=487, Percent_Identity=36.7556468172485, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI71984413, Length=486, Percent_Identity=36.8312757201646, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI71984406, Length=486, Percent_Identity=36.8312757201646, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17506829, Length=486, Percent_Identity=36.8312757201646, Blast_Score=238, Evalue=4e-63,
Organism=Caenorhabditis elegans, GI17506831, Length=486, Percent_Identity=36.8312757201646, Blast_Score=238, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=493, Percent_Identity=33.8742393509128, Blast_Score=245, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=432, Percent_Identity=35.4166666666667, Blast_Score=233, Evalue=6e-62,
Organism=Drosophila melanogaster, GI24648964, Length=493, Percent_Identity=36.7139959432049, Blast_Score=258, Evalue=8e-69,
Organism=Drosophila melanogaster, GI28571814, Length=493, Percent_Identity=36.7139959432049, Blast_Score=257, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24648966, Length=413, Percent_Identity=37.2881355932203, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24581235, Length=495, Percent_Identity=32.5252525252525, Blast_Score=224, Evalue=9e-59,
Organism=Drosophila melanogaster, GI24646914, Length=274, Percent_Identity=35.7664233576642, Blast_Score=135, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 50969; Mature: 50838

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMHRATKIVATLGPASSTPDVLERMITAGVDVVRLNFSHGSAQDHIARAALVREVAQRA
CCCHHHHEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHC
GKEVAIMADLQGPKIRVGKFAQGKVMLEPGAAFVLDAARSEPGDLQAVGLDYKSLPRDVK
CCEEEEEEECCCCEEEECCEECCEEEECCCCEEEEECCCCCCCCEEEECCCHHHCCCCCC
PGDTLLLNDGLIVLTVDKVIGEAVHTVVKIGGELSNNKGINKQGGGLTAPALTAKDMEDI
CCCEEEEECCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
KTAMSFQCEYLAVSFPKSATDMEMARQLANVAGEPWRHKPALIAKIERSEAIPVLEQILK
HHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCHHHHHHHH
ASDGIMVARGDLAVEVGNAAVPALQKKMIKLARELDKVVITATQMMESMIVNPVPTRAEV
CCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
SDVANAVLDGTDAVMLSAETAAGKYPVETIEQMAAIALAAERAEDVALDADFTNKRFGRI
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
DQSIAMGALFTAHHLGCKAIVALTESGSTALWMSRHKIHVPIFALTSQQTSQRKMTLYRN
HHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHC
VRPLLMPSYTDRDEALAKAEALLVDGGVLKPGDTYAITCGEPMGYPGGTNMLKVCRVA
CCCCCCCCCCCHHHHHHHHHEEEECCCEECCCCCEEEECCCCCCCCCCCCHHHHEECC
>Mature Secondary Structure 
TMHRATKIVATLGPASSTPDVLERMITAGVDVVRLNFSHGSAQDHIARAALVREVAQRA
CCHHHHEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHC
GKEVAIMADLQGPKIRVGKFAQGKVMLEPGAAFVLDAARSEPGDLQAVGLDYKSLPRDVK
CCEEEEEEECCCCEEEECCEECCEEEECCCCEEEEECCCCCCCCEEEECCCHHHCCCCCC
PGDTLLLNDGLIVLTVDKVIGEAVHTVVKIGGELSNNKGINKQGGGLTAPALTAKDMEDI
CCCEEEEECCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
KTAMSFQCEYLAVSFPKSATDMEMARQLANVAGEPWRHKPALIAKIERSEAIPVLEQILK
HHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCHHEEEECCCCCCHHHHHHHH
ASDGIMVARGDLAVEVGNAAVPALQKKMIKLARELDKVVITATQMMESMIVNPVPTRAEV
CCCCEEEEECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
SDVANAVLDGTDAVMLSAETAAGKYPVETIEQMAAIALAAERAEDVALDADFTNKRFGRI
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHH
DQSIAMGALFTAHHLGCKAIVALTESGSTALWMSRHKIHVPIFALTSQQTSQRKMTLYRN
HHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEECCEEEEEEEEECCCCCHHHHHHHHHC
VRPLLMPSYTDRDEALAKAEALLVDGGVLKPGDTYAITCGEPMGYPGGTNMLKVCRVA
CCCCCCCCCCCHHHHHHHHHEEEECCCEECCCCCEEEECCCCCCCCCCCCHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]