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The map label for this gene is dmlA [H]

Identifier: 124265444

GI number: 124265444

Start: 273006

End: 274124

Strand: Direct

Name: dmlA [H]

Synonym: Mpe_A0251

Alternate gene names: 124265444

Gene position: 273006-274124 (Clockwise)

Preceding gene: 124265443

Following gene: 124265446

Centisome position: 6.75

GC content: 69.97

Gene sequence:

>1119_bases
ATGAAGCCCCATCGCATTGCGGTGATTCCCGGCGACGGCATCGGGCAGGAGGTGATGCCGGAGGGCCTGCGCGTGCTGGA
GGCCGCGGCGCAGCGCTTCGAACTGCCGCTGCAGTTCGATCATTTCGACTGGGCCTGCGCCGCCTACTGGCAGCGCACCG
GGCAGATGCTGCCCGACGACTGGTTCGCGACGCTGAGCGGCTACGACGCCATCTACTTCGGCGCGGTCGGCTGGCCGGCG
GTGGTGCCCGACCACGTGTCGCTGTGGGGCTCGCTGCTCAAGTTCCGCCGCGAGTTCGACCAGTACGTCAACCTGCGCCC
GGTGCGGCTGTTCGACGGCGTGCCGTGTCCGCTGGTCGACCGCGACGGCCGGCCGCGCAAGCCCGGCGACATCGACTTCT
TCGTGGTGCGCGAGAACACCGAGGGCGAGTACACCCATCTCGGCGGGCGCCTCTTCGAAGGCACCGAGCGCGAGATCGTG
ATCCAGGAGTCGGTGTTCTCGCGCCACGGCACCGACCGGGTGATGAAGTTCGCCTTCGAGCTGGCGCAGTCGCGGCCGCG
CAAGCACCTCACCGTCGCCACCAAGTCCAACGGCATCGCCATCAGCATGCCGTGGTGGGACGAACGCGCCGACGCGATGG
CGCAGGGCTACCCCGAGGTCACGGTCGACAAGCAGCACATCGACATCCTCAGCGCGCGCTTCGTGCTGCAGCCGACTCGC
TTCGACGTCGTGGTCGCCAGCAACCTGTTCGGCGACATCCTCAGCGACCTGGGCCCGGCCTGCACCGGCACCATCGGCCT
GGCGCCCTCGGCGAACCTGAATCCGCAGCGCCATTTCCCCTCGTTGTTCGAGCCGGTGCACGGCTCGGCGCCCGACATCT
TCGGTCGCGCGATCGCGAACCCGGTGGCGATGATCTGGTCGGGCGCGCTGATGCTCGACTTCCTCGGCGGCGCGATCGGT
GCGGACACCGCACGCCATCTGCAGGCCCACGACGCCATCGTGGCGGCGATCGAGCGGGTGCTGGTGACCGGGCCGCGCAC
GCCCGACCTGGGTGGCCACGCCTCGACGACCGTGATGGGGCGCGCGATCGCCGAGCAGGTGGCGGCCGGCGCGCCTTAG

Upstream 100 bases:

>100_bases
CGGCCAGCTCGGTATTCGAGGAGGCCTACACGCGCGGCACACTGGCGGGCCAGGCGGTCGCGCAGGCCCTGGGCGGCACC
GAGACTTCGAGGAGACCGAC

Downstream 100 bases:

>100_bases
GCGCGTTGGCTCAGCGCGCGCCGGCGCGTGTCAGCCGGTCGAGTTCGGCGCAGGCCGCGGCCTTGCCCTCGTTGAACACC
ACCACCTCGTTCGACAGCAA

Product: tartrate dehydrogenase

Products: NA

Alternate protein names: D-malate degradation protein A; D-malate oxidase [H]

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MKPHRIAVIPGDGIGQEVMPEGLRVLEAAAQRFELPLQFDHFDWACAAYWQRTGQMLPDDWFATLSGYDAIYFGAVGWPA
VVPDHVSLWGSLLKFRREFDQYVNLRPVRLFDGVPCPLVDRDGRPRKPGDIDFFVVRENTEGEYTHLGGRLFEGTEREIV
IQESVFSRHGTDRVMKFAFELAQSRPRKHLTVATKSNGIAISMPWWDERADAMAQGYPEVTVDKQHIDILSARFVLQPTR
FDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPQRHFPSLFEPVHGSAPDIFGRAIANPVAMIWSGALMLDFLGGAIG
ADTARHLQAHDAIVAAIERVLVTGPRTPDLGGHASTTVMGRAIAEQVAAGAP

Sequences:

>Translated_372_residues
MKPHRIAVIPGDGIGQEVMPEGLRVLEAAAQRFELPLQFDHFDWACAAYWQRTGQMLPDDWFATLSGYDAIYFGAVGWPA
VVPDHVSLWGSLLKFRREFDQYVNLRPVRLFDGVPCPLVDRDGRPRKPGDIDFFVVRENTEGEYTHLGGRLFEGTEREIV
IQESVFSRHGTDRVMKFAFELAQSRPRKHLTVATKSNGIAISMPWWDERADAMAQGYPEVTVDKQHIDILSARFVLQPTR
FDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPQRHFPSLFEPVHGSAPDIFGRAIANPVAMIWSGALMLDFLGGAIG
ADTARHLQAHDAIVAAIERVLVTGPRTPDLGGHASTTVMGRAIAEQVAAGAP
>Mature_372_residues
MKPHRIAVIPGDGIGQEVMPEGLRVLEAAAQRFELPLQFDHFDWACAAYWQRTGQMLPDDWFATLSGYDAIYFGAVGWPA
VVPDHVSLWGSLLKFRREFDQYVNLRPVRLFDGVPCPLVDRDGRPRKPGDIDFFVVRENTEGEYTHLGGRLFEGTEREIV
IQESVFSRHGTDRVMKFAFELAQSRPRKHLTVATKSNGIAISMPWWDERADAMAQGYPEVTVDKQHIDILSARFVLQPTR
FDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPQRHFPSLFEPVHGSAPDIFGRAIANPVAMIWSGALMLDFLGGAIG
ADTARHLQAHDAIVAAIERVLVTGPRTPDLGGHASTTVMGRAIAEQVAAGAP

Specific function: Catalyzes the NAD(+)-dependent oxidative decarboxylation of D-malate into pyruvate. Is essential for aerobic growth on D- malate as the sole carbon source. But is not required for anaerobic D-malate utilization, although DmlA is expressed and active in th

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=365, Percent_Identity=29.3150684931507, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI4758582, Length=357, Percent_Identity=28.0112044817927, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI28178821, Length=356, Percent_Identity=25, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI28178838, Length=350, Percent_Identity=28, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI28178816, Length=355, Percent_Identity=24.7887323943662, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI28178819, Length=211, Percent_Identity=27.9620853080569, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788101, Length=367, Percent_Identity=69.2098092643052, Blast_Score=533, Evalue=1e-152,
Organism=Escherichia coli, GI87081683, Length=381, Percent_Identity=39.3700787401575, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1787381, Length=358, Percent_Identity=26.536312849162, Blast_Score=110, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17550882, Length=366, Percent_Identity=27.8688524590164, Blast_Score=145, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71986051, Length=356, Percent_Identity=31.1797752808989, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=364, Percent_Identity=25.8241758241758, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17505779, Length=366, Percent_Identity=25.9562841530055, Blast_Score=106, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322097, Length=374, Percent_Identity=36.096256684492, Blast_Score=191, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6319830, Length=376, Percent_Identity=33.2446808510638, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6324709, Length=369, Percent_Identity=29.5392953929539, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324291, Length=372, Percent_Identity=29.3010752688172, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24643270, Length=374, Percent_Identity=31.0160427807487, Blast_Score=165, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24643268, Length=374, Percent_Identity=31.0160427807487, Blast_Score=164, Evalue=6e-41,
Organism=Drosophila melanogaster, GI24661184, Length=372, Percent_Identity=28.7634408602151, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI161078635, Length=356, Percent_Identity=27.8089887640449, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078637, Length=368, Percent_Identity=27.445652173913, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078633, Length=368, Percent_Identity=27.445652173913, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24650122, Length=368, Percent_Identity=27.445652173913, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161078639, Length=279, Percent_Identity=31.1827956989247, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI281362242, Length=370, Percent_Identity=27.2972972972973, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24648872, Length=370, Percent_Identity=27.2972972972973, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20130355, Length=369, Percent_Identity=23.8482384823848, Blast_Score=96, Evalue=5e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011829 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.83 [H]

Molecular weight: Translated: 40860; Mature: 40860

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPHRIAVIPGDGIGQEVMPEGLRVLEAAAQRFELPLQFDHFDWACAAYWQRTGQMLPDD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCH
WFATLSGYDAIYFGAVGWPAVVPDHVSLWGSLLKFRREFDQYVNLRPVRLFDGVPCPLVD
HHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
RDGRPRKPGDIDFFVVRENTEGEYTHLGGRLFEGTEREIVIQESVFSRHGTDRVMKFAFE
CCCCCCCCCCEEEEEEEECCCCCEEECCCEEECCCCCEEEEHHHHHHHCCHHHHHHHHHH
LAQSRPRKHLTVATKSNGIAISMPWWDERADAMAQGYPEVTVDKQHIDILSARFVLQPTR
HHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHCCCCCEEECHHHHHHHHHHEEECCCC
FDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPQRHFPSLFEPVHGSAPDIFGRAIAN
EEEEEHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHCCCHHHHCCCCCCCHHHHHHHHH
PVAMIWSGALMLDFLGGAIGADTARHLQAHDAIVAAIERVLVTGPRTPDLGGHASTTVMG
HHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
RAIAEQVAAGAP
HHHHHHHHCCCC
>Mature Secondary Structure
MKPHRIAVIPGDGIGQEVMPEGLRVLEAAAQRFELPLQFDHFDWACAAYWQRTGQMLPDD
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCH
WFATLSGYDAIYFGAVGWPAVVPDHVSLWGSLLKFRREFDQYVNLRPVRLFDGVPCPLVD
HHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
RDGRPRKPGDIDFFVVRENTEGEYTHLGGRLFEGTEREIVIQESVFSRHGTDRVMKFAFE
CCCCCCCCCCEEEEEEEECCCCCEEECCCEEECCCCCEEEEHHHHHHHCCHHHHHHHHHH
LAQSRPRKHLTVATKSNGIAISMPWWDERADAMAQGYPEVTVDKQHIDILSARFVLQPTR
HHHCCCCCEEEEEECCCCEEEECCCCCHHHHHHHCCCCCEEECHHHHHHHHHHEEECCCC
FDVVVASNLFGDILSDLGPACTGTIGLAPSANLNPQRHFPSLFEPVHGSAPDIFGRAIAN
EEEEEHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCHHCCCHHHHCCCCCCCHHHHHHHHH
PVAMIWSGALMLDFLGGAIGADTARHLQAHDAIVAAIERVLVTGPRTPDLGGHASTTVMG
HHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
RAIAEQVAAGAP
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]