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The map label for this gene is speE1 [H]

Identifier: 124265442

GI number: 124265442

Start: 269756

End: 271342

Strand: Direct

Name: speE1 [H]

Synonym: Mpe_A0249

Alternate gene names: 124265442

Gene position: 269756-271342 (Clockwise)

Preceding gene: 124265441

Following gene: 124265443

Centisome position: 6.67

GC content: 69.94

Gene sequence:

>1587_bases
GTGAGCGAACCGGCGGCACTCGATGGCGCGGCCGACGGCCGCGCGGCGGCAGCGGTGCGACCGGCCGAGGTGGCCTTGCT
CGCCTCGGTGTTCGTCGTCGCGGCCTGCGGCCTGGTCTACGAGCTCGCAGCAGGGGCGCTGGCGAGCTACCTGCTCGGCG
ACTCGGTCCTGCAATTCAGCACCGTCATCGGCGCCTACCTGTTCGCGATGGGCATCGGCTCCTGGCTGTCGCGCCACATC
GAGCGCCAACTGGTCGCGAACTTCCTGCGCATCGAGCTGCTGATCGGCCTGATCGGCGGGCTGATGCCGGCGGCACTGTT
CGTCGCGCACAGCCTGCTGCCGGTCAGCGCGGTCGCGCCCTTCCGTGCCCTGCTCTACGGGCTGGTGCTGGCGGTGGGCA
TCCTGGTGGGCCTGGAGATCCCGCTGGTGATGCGCCTGCTCAAGCGCCACTTCAGCGAGCGTTACGCGCTGAAGGACCTG
GTGTCGCAGGTGCTGACCTTCGACTATCTCGGAGCGCTGGCCGTGTCGGTCGCGTTCCCGCTGCTGCTCGTGCCGCAGCT
CGGGCTGGTCCGCACCGGCGTGGTGTTCGGCCTGCTGAACGTCGTCGTCGCGGTGTGGGCGCTGTGGCTCTTCCGGGCAG
AACTGAGGGCCTGGCGCGCCCATGCGCTGGCCTGCGCTGCCGTGCTCGCGGCGCTCGGTGCGGCGCTGATCGGGGCCGAC
CGGCTCACGACCTGGGCTGAGGACCGCTTCTACGGCGACCGCATCGTCTTCAGCCAGAGCTCGCCCTACCAGCGCGTCGT
CGTCACGTCGGGGGCGGCCGGCGTGCGGCTGTTCCTCAACGGCAACCTGCAGTTCCACTCGCGCGACGAGTACCGCTACC
ACGAGGCGCTGGTGCATCCGGCGATGGCGGCGCATGGCGCGCCCAAGCGGGTTCTGGTGCTCGGCGGCGGCGACGGCATG
GCGGTGCGCGAGGTGCTGAAGCACTCGACGGTCGAGCACGTCACGCTGGTCGAGCTGGACCCGGCGATGACGCGCCTGTT
CTCGACCGAACCGCTGCTGATCGCACTGAATGGCGGGGCGCTGAAGTCGCCCAAGCTCACGATCGTCAACGACGATGCCT
TTGGCTGGCTGGACCGCCACGACGCGCTGTTCGACGTGATCGTGGTCGACTTCCCCGACCCCAGCAACCACGCGATCGGC
AAGCTCTACTCGACCAGCTTCTACGCGCTGATGGCCCGCCATCTCGCGGCCTCCGGCTACGCGGTGGTGCAGACCACCTC
GCCGCTGATCGCGCGCAAGAGCTTCTGGACGGTGGTGAGCACCATCGAGGCCGTGGGCCTGCAGGCGTCGCCCTATCACG
CGCACGTGCCGAGCTTCGGCGAATGGGGCTTCGTGTTGGCCGGTCAACGTCCGCTGGCCACGCCGCGCCGACTGCCCGAG
GGCTTGCGCTTCCTGACCGTCGAGGGCCTGCCCGCGCTGTTCGACTTCCCGCCCGACATGGCCCGCGTGCCCGCCGAGCC
CAACCGCCTCAGCACCCAGGGGCTGGTGCACACCTTCGAAGAGGAATGGGGTCGCGTCCACCAGTGA

Upstream 100 bases:

>100_bases
GCTGGCACTGGTGGTGGCGGCGATGTGCCTGGCGATCGGGCTGATCGTCGCCGCGGCGATCCATGGCTGACCGCGCGCAC
CCGGCGCAGCCGGCCGGCGC

Downstream 100 bases:

>100_bases
GTGCCGCACGCCGTGCGCTGCTGGCGGCCGGCGGCGCGCTGGCCACCTGGCCGTGGCTGGCCGGTTGCGAGCGCGCGCCG
GTCGAGGTGGCGGGCGGTTG

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 1; PAPT 1; SPDSY 1 [H]

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSEPAALDGAADGRAAAAVRPAEVALLASVFVVAACGLVYELAAGALASYLLGDSVLQFSTVIGAYLFAMGIGSWLSRHI
ERQLVANFLRIELLIGLIGGLMPAALFVAHSLLPVSAVAPFRALLYGLVLAVGILVGLEIPLVMRLLKRHFSERYALKDL
VSQVLTFDYLGALAVSVAFPLLLVPQLGLVRTGVVFGLLNVVVAVWALWLFRAELRAWRAHALACAAVLAALGAALIGAD
RLTTWAEDRFYGDRIVFSQSSPYQRVVVTSGAAGVRLFLNGNLQFHSRDEYRYHEALVHPAMAAHGAPKRVLVLGGGDGM
AVREVLKHSTVEHVTLVELDPAMTRLFSTEPLLIALNGGALKSPKLTIVNDDAFGWLDRHDALFDVIVVDFPDPSNHAIG
KLYSTSFYALMARHLAASGYAVVQTTSPLIARKSFWTVVSTIEAVGLQASPYHAHVPSFGEWGFVLAGQRPLATPRRLPE
GLRFLTVEGLPALFDFPPDMARVPAEPNRLSTQGLVHTFEEEWGRVHQ

Sequences:

>Translated_528_residues
MSEPAALDGAADGRAAAAVRPAEVALLASVFVVAACGLVYELAAGALASYLLGDSVLQFSTVIGAYLFAMGIGSWLSRHI
ERQLVANFLRIELLIGLIGGLMPAALFVAHSLLPVSAVAPFRALLYGLVLAVGILVGLEIPLVMRLLKRHFSERYALKDL
VSQVLTFDYLGALAVSVAFPLLLVPQLGLVRTGVVFGLLNVVVAVWALWLFRAELRAWRAHALACAAVLAALGAALIGAD
RLTTWAEDRFYGDRIVFSQSSPYQRVVVTSGAAGVRLFLNGNLQFHSRDEYRYHEALVHPAMAAHGAPKRVLVLGGGDGM
AVREVLKHSTVEHVTLVELDPAMTRLFSTEPLLIALNGGALKSPKLTIVNDDAFGWLDRHDALFDVIVVDFPDPSNHAIG
KLYSTSFYALMARHLAASGYAVVQTTSPLIARKSFWTVVSTIEAVGLQASPYHAHVPSFGEWGFVLAGQRPLATPRRLPE
GLRFLTVEGLPALFDFPPDMARVPAEPNRLSTQGLVHTFEEEWGRVHQ
>Mature_527_residues
SEPAALDGAADGRAAAAVRPAEVALLASVFVVAACGLVYELAAGALASYLLGDSVLQFSTVIGAYLFAMGIGSWLSRHIE
RQLVANFLRIELLIGLIGGLMPAALFVAHSLLPVSAVAPFRALLYGLVLAVGILVGLEIPLVMRLLKRHFSERYALKDLV
SQVLTFDYLGALAVSVAFPLLLVPQLGLVRTGVVFGLLNVVVAVWALWLFRAELRAWRAHALACAAVLAALGAALIGADR
LTTWAEDRFYGDRIVFSQSSPYQRVVVTSGAAGVRLFLNGNLQFHSRDEYRYHEALVHPAMAAHGAPKRVLVLGGGDGMA
VREVLKHSTVEHVTLVELDPAMTRLFSTEPLLIALNGGALKSPKLTIVNDDAFGWLDRHDALFDVIVVDFPDPSNHAIGK
LYSTSFYALMARHLAASGYAVVQTTSPLIARKSFWTVVSTIEAVGLQASPYHAHVPSFGEWGFVLAGQRPLATPRRLPEG
LRFLTVEGLPALFDFPPDMARVPAEPNRLSTQGLVHTFEEEWGRVHQ

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=222, Percent_Identity=28.8288288288288, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786312, Length=177, Percent_Identity=33.8983050847458, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI212645616, Length=224, Percent_Identity=35.2678571428571, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25154106, Length=255, Percent_Identity=35.2941176470588, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6323175, Length=238, Percent_Identity=31.0924369747899, Blast_Score=110, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6325326, Length=176, Percent_Identity=34.6590909090909, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645443, Length=236, Percent_Identity=33.4745762711864, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355941, Length=187, Percent_Identity=34.7593582887701, Blast_Score=87, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 56999; Mature: 56868

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEECCCCHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SEPAALDGAADGRAAAAVRPAEVALLASVFVVAACGLVYELAAGALASYLLGDSVLQFS
CCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVIGAYLFAMGIGSWLSRHIERQLVANFLRIELLIGLIGGLMPAALFVAHSLLPVSAVAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRALLYGLVLAVGILVGLEIPLVMRLLKRHFSERYALKDLVSQVLTFDYLGALAVSVAFP
HHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLLVPQLGLVRTGVVFGLLNVVVAVWALWLFRAELRAWRAHALACAAVLAALGAALIGAD
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLTTWAEDRFYGDRIVFSQSSPYQRVVVTSGAAGVRLFLNGNLQFHSRDEYRYHEALVHP
HHHHHHHHCCCCCEEEEECCCCCCEEEEECCCCCEEEEEECCEEECCCCHHHHHHHHHHH
AMAAHGAPKRVLVLGGGDGMAVREVLKHSTVEHVTLVELDPAMTRLFSTEPLLIALNGGA
HHHHCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEEECHHHHHHHCCCCEEEEECCCC
LKSPKLTIVNDDAFGWLDRHDALFDVIVVDFPDPSNHAIGKLYSTSFYALMARHLAASGY
CCCCEEEEEECCCCCCHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCE
AVVQTTSPLIARKSFWTVVSTIEAVGLQASPYHAHVPSFGEWGFVLAGQRPLATPRRLPE
EEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCHHHCCC
GLRFLTVEGLPALFDFPPDMARVPAEPNRLSTQGLVHTFEEEWGRVHQ
CCEEEEECCCCHHHCCCCCHHCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]