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The map label for this gene is yafV [C]

Identifier: 124265437

GI number: 124265437

Start: 264548

End: 265366

Strand: Direct

Name: yafV [C]

Synonym: Mpe_A0244

Alternate gene names: 124265437

Gene position: 264548-265366 (Clockwise)

Preceding gene: 124265436

Following gene: 124265439

Centisome position: 6.54

GC content: 73.75

Gene sequence:

>819_bases
ATGAAGATCGCAGCCGTGCAGATGGTGTCCACGCCGCGCGTCGCGGAGAACCTGGCGCGGGCCCGGGCGCTGATCGGCCG
GGCGGCCGACGCCGGGGCCGAGCTGGTGGCCCTGCCGGAGTACTTCTGCCTGATGGGCCAGCGCGACACCGACAAGCTCG
ACGTGGCCGAGGCCGACGGCAGCGGCCCGATTCAGGACCTGCTGGCCGCCGCTGCCCGCGAGTGCGGCGTGTGGCTGGTG
GGCGGCACGCTGCCCTTGCGCATCGACGGGCGCCGCGACCGGGCCCACAACACCACGCTGGTGTTCGGCCCCGACGGCCG
GCGCGTGGCGCGCTACGACAAGGTCCACCTGTTCCGCTTCGACGACGGCGAGCGACGCTACGACGAGGCCGCCACGCTGC
TGCCGGGCGCCACGCCGGTAGCCATCGCGCTCACCGACCGCGCCGGGCACACCTGGCGTGTCGGCCTCAGCATCTGCTAC
GACCTGCGCTTTCCCGAGCTGTACCGCGCGCTCTGCGTGCCGCCCTGCGACCTGCTGCTGGTGCCGGCCGCCTTCACCTA
CCCCACCGGCCAGGCGCACTGGGAGCTGTTGCTGCGCGCGCGGGCGGTCGAGAACCAGTGCATGGTGCTGGCGCCGGCGC
AGGGCGGCCGCCACGAGAACGGCCGCCGCACCTGGGGTCACACGATGATCGTCGGCCCCTGGGGCGACGTACTGGCGGTG
CAGGCCGAGGACGAGGGTGTGGTGATCGCGAGCCTCGACGCTGCCCGCATGGCGCAGGTGCGCGCGCAACTGCCTGCGCT
GCAGCACCGCGTGCTGTAG

Upstream 100 bases:

>100_bases
GACCGTGGGCCGAGCCCAAGGTCGAGCGCATCGAGCGCAAACTCGGCGACGCGGTGCCGACCGACGAGGCGGCCTCGGCG
CCGGCGTCCAAGGAGTCGCC

Downstream 100 bases:

>100_bases
CGCCGCTGCACGCGGCCGCGTCAGCGCCAGCCGAACATCATCTGCCGAGCCAGCAGTCGCTTGGCCGGCGACAACGCCTG
CAGCGCCGCCAGACCCAGCC

Product: putative nitrilase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKIAAVQMVSTPRVAENLARARALIGRAADAGAELVALPEYFCLMGQRDTDKLDVAEADGSGPIQDLLAAAARECGVWLV
GGTLPLRIDGRRDRAHNTTLVFGPDGRRVARYDKVHLFRFDDGERRYDEAATLLPGATPVAIALTDRAGHTWRVGLSICY
DLRFPELYRALCVPPCDLLLVPAAFTYPTGQAHWELLLRARAVENQCMVLAPAQGGRHENGRRTWGHTMIVGPWGDVLAV
QAEDEGVVIASLDAARMAQVRAQLPALQHRVL

Sequences:

>Translated_272_residues
MKIAAVQMVSTPRVAENLARARALIGRAADAGAELVALPEYFCLMGQRDTDKLDVAEADGSGPIQDLLAAAARECGVWLV
GGTLPLRIDGRRDRAHNTTLVFGPDGRRVARYDKVHLFRFDDGERRYDEAATLLPGATPVAIALTDRAGHTWRVGLSICY
DLRFPELYRALCVPPCDLLLVPAAFTYPTGQAHWELLLRARAVENQCMVLAPAQGGRHENGRRTWGHTMIVGPWGDVLAV
QAEDEGVVIASLDAARMAQVRAQLPALQHRVL
>Mature_272_residues
MKIAAVQMVSTPRVAENLARARALIGRAADAGAELVALPEYFCLMGQRDTDKLDVAEADGSGPIQDLLAAAARECGVWLV
GGTLPLRIDGRRDRAHNTTLVFGPDGRRVARYDKVHLFRFDDGERRYDEAATLLPGATPVAIALTDRAGHTWRVGLSICY
DLRFPELYRALCVPPCDLLLVPAAFTYPTGQAHWELLLRARAVENQCMVLAPAQGGRHENGRRTWGHTMIVGPWGDVLAV
QAEDEGVVIASLDAARMAQVRAQLPALQHRVL

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=271, Percent_Identity=35.4243542435424, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI297632350, Length=271, Percent_Identity=37.6383763837638, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI297632348, Length=271, Percent_Identity=37.6383763837638, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI5031947, Length=271, Percent_Identity=37.6383763837638, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI297632346, Length=196, Percent_Identity=33.1632653061224, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1786412, Length=238, Percent_Identity=29.8319327731092, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17556280, Length=278, Percent_Identity=37.410071942446, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6323383, Length=279, Percent_Identity=32.6164874551971, Blast_Score=143, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6322335, Length=303, Percent_Identity=31.023102310231, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI17933642, Length=281, Percent_Identity=37.7224199288256, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI21355835, Length=275, Percent_Identity=33.8181818181818, Blast_Score=147, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 29690; Mature: 29690

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAAVQMVSTPRVAENLARARALIGRAADAGAELVALPEYFCLMGQRDTDKLDVAEADG
CCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCEEEEECCC
SGPIQDLLAAAARECGVWLVGGTLPLRIDGRRDRAHNTTLVFGPDGRRVARYDKVHLFRF
CCCHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCCEEEECCCCCEEEEECEEEEEEE
DDGERRYDEAATLLPGATPVAIALTDRAGHTWRVGLSICYDLRFPELYRALCVPPCDLLL
CCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHCCCCCCEEE
VPAAFTYPTGQAHWELLLRARAVENQCMVLAPAQGGRHENGRRTWGHTMIVGPWGDVLAV
ECCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEECCCEEEEECCCCEEEE
QAEDEGVVIASLDAARMAQVRAQLPALQHRVL
EECCCCEEEEECCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKIAAVQMVSTPRVAENLARARALIGRAADAGAELVALPEYFCLMGQRDTDKLDVAEADG
CCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCEEEEECCC
SGPIQDLLAAAARECGVWLVGGTLPLRIDGRRDRAHNTTLVFGPDGRRVARYDKVHLFRF
CCCHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCCCCEEEECCCCCEEEEECEEEEEEE
DDGERRYDEAATLLPGATPVAIALTDRAGHTWRVGLSICYDLRFPELYRALCVPPCDLLL
CCCCHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEEEEEEECCCHHHHHHHCCCCCCEEE
VPAAFTYPTGQAHWELLLRARAVENQCMVLAPAQGGRHENGRRTWGHTMIVGPWGDVLAV
ECCEECCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCEECCCEEEEECCCCEEEE
QAEDEGVVIASLDAARMAQVRAQLPALQHRVL
EECCCCEEEEECCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]