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The map label for this gene is garL [H]

Identifier: 124265382

GI number: 124265382

Start: 205543

End: 206382

Strand: Direct

Name: garL [H]

Synonym: Mpe_A0189

Alternate gene names: 124265382

Gene position: 205543-206382 (Clockwise)

Preceding gene: 124265381

Following gene: 124265383

Centisome position: 5.08

GC content: 72.62

Gene sequence:

>840_bases
ATGAATGCGTTCAAGCAGCTGTTGCAGAACCCGGCCCGTGGCATCGACGCGCACCCGCGCGCCAGCCACCAGCGCGTGCC
GCTCGGCACCTGGGTCATGTCGGCCAGCCCGCTGGTTGCCGAGGCGATCGGCCATGCAGGCTTCGACTGGGCGGTGCTCG
ACATGGAGCACTCGCCGCTCGAGCTGCCGGGCGTGGTGCAGATGCTGCAAGCCCTGGCCACGACGAAGCTGGTGCCGATC
GTGCGCCTGCCCGCGAACGATGCGGTGCTGTTCAAGCGCCTGCTCGATGCGGGTGCCAGCACCTTGATGGTGCCGTTCGT
CCAGAGCGCCGCCGAGGCCCGGCGCGCCGTGGCGGCCACGCGCTATCCGCCGCTCGGCATGCGCGGTGTGGCCGGCATGA
GCCGCGCCACGCGCTACGGCACGCTGCGCCTCGATACGCAGGCGGCCGACGCGGGCGTGGCTCTGATCCTGCAGCTCGAA
ACGCCGCAGGCGCTCGACGCGCTGGAGGAGATCGCCGCGGTCGAGGGCGTCGATGCGCTGTTCATCGGGCCGGCCGATCT
TTCGGCGGCCCTTGGGCATGGCGGCAATGCGCGGCACCCGGCGGTGCAGAAGCTGGCAGCGCAGGCGGCGGAGCGCGCGC
GTGCGGCGGGCATCCCGATCGGCACGCTGGCCGGCACGCCGGAAATGGCCACCCAGGTCCGCGCGGCCGGGTTCGATTTC
GTCGGCCTGGGGTCCGACCTCGGGTTGCTGGTGCATGCGGCGCAGGCCTCGCTGCACGCGCTGCGCACGCCGGATGCCGC
GGCGGTGCACTCGCTCCACGTCGGGACGCACGCCTACTGA

Upstream 100 bases:

>100_bases
ACCTGCGGGTCGGCCTCGAAGGGTCCGGCTCGCTGTACTGAGTCCCAGATCGTTAAGTGGAGTGTCGAGGCGGCGCCGTT
GCGTCGCCCGGGAGGCCGCG

Downstream 100 bases:

>100_bases
CCCGCGGGAGGGCCGCGGCCGAAGCAGGCTGGGGCGGCTGGCCCCTTAACACGGCAGCGGGCGTCCGTCGAGAGGCTTTC
GCGCACGCGAATCAGCGGAC

Product: 2-dehydro-3-deoxyglucarate aldolase

Products: NA

Alternate protein names: KDGluc aldolase; KDGlucA; 2-dehydro-3-deoxy-D-glucarate aldolase; 2-keto-3-deoxy-D-glucarate aldolase; 5-dehydro-4-deoxy-D-glucarate aldolase; Alpha-keto-beta-deoxy-D-glucarate aldolase [H]

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MNAFKQLLQNPARGIDAHPRASHQRVPLGTWVMSASPLVAEAIGHAGFDWAVLDMEHSPLELPGVVQMLQALATTKLVPI
VRLPANDAVLFKRLLDAGASTLMVPFVQSAAEARRAVAATRYPPLGMRGVAGMSRATRYGTLRLDTQAADAGVALILQLE
TPQALDALEEIAAVEGVDALFIGPADLSAALGHGGNARHPAVQKLAAQAAERARAAGIPIGTLAGTPEMATQVRAAGFDF
VGLGSDLGLLVHAAQASLHALRTPDAAAVHSLHVGTHAY

Sequences:

>Translated_279_residues
MNAFKQLLQNPARGIDAHPRASHQRVPLGTWVMSASPLVAEAIGHAGFDWAVLDMEHSPLELPGVVQMLQALATTKLVPI
VRLPANDAVLFKRLLDAGASTLMVPFVQSAAEARRAVAATRYPPLGMRGVAGMSRATRYGTLRLDTQAADAGVALILQLE
TPQALDALEEIAAVEGVDALFIGPADLSAALGHGGNARHPAVQKLAAQAAERARAAGIPIGTLAGTPEMATQVRAAGFDF
VGLGSDLGLLVHAAQASLHALRTPDAAAVHSLHVGTHAY
>Mature_279_residues
MNAFKQLLQNPARGIDAHPRASHQRVPLGTWVMSASPLVAEAIGHAGFDWAVLDMEHSPLELPGVVQMLQALATTKLVPI
VRLPANDAVLFKRLLDAGASTLMVPFVQSAAEARRAVAATRYPPLGMRGVAGMSRATRYGTLRLDTQAADAGVALILQLE
TPQALDALEEIAAVEGVDALFIGPADLSAALGHGGNARHPAVQKLAAQAAERARAAGIPIGTLAGTPEMATQVRAAGFDF
VGLGSDLGLLVHAAQASLHALRTPDAAAVHSLHVGTHAY

Specific function: Catalyzes the reversible retro-aldol cleavage of both 5- keto-4-deoxy-D-glucarate and 2-keto-3-deoxy-D-glucarate to pyruvate and tartronic semialdehyde [H]

COG id: COG3836

COG function: function code G; 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. KDGluc aldolase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789514, Length=232, Percent_Identity=43.5344827586207, Blast_Score=200, Evalue=9e-53,
Organism=Escherichia coli, GI1788578, Length=264, Percent_Identity=40.530303030303, Blast_Score=186, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR017648
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: =4.1.2.20 [H]

Molecular weight: Translated: 28967; Mature: 28967

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAFKQLLQNPARGIDAHPRASHQRVPLGTWVMSASPLVAEAIGHAGFDWAVLDMEHSPL
CCHHHHHHHCHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCC
ELPGVVQMLQALATTKLVPIVRLPANDAVLFKRLLDAGASTLMVPFVQSAAEARRAVAAT
CCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RYPPLGMRGVAGMSRATRYGTLRLDTQAADAGVALILQLETPQALDALEEIAAVEGVDAL
CCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEE
FIGPADLSAALGHGGNARHPAVQKLAAQAAERARAAGIPIGTLAGTPEMATQVRAAGFDF
EECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCCE
VGLGSDLGLLVHAAQASLHALRTPDAAAVHSLHVGTHAY
EECCCCHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCC
>Mature Secondary Structure
MNAFKQLLQNPARGIDAHPRASHQRVPLGTWVMSASPLVAEAIGHAGFDWAVLDMEHSPL
CCHHHHHHHCHHCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCC
ELPGVVQMLQALATTKLVPIVRLPANDAVLFKRLLDAGASTLMVPFVQSAAEARRAVAAT
CCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
RYPPLGMRGVAGMSRATRYGTLRLDTQAADAGVALILQLETPQALDALEEIAAVEGVDAL
CCCCCCCHHHHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCCEE
FIGPADLSAALGHGGNARHPAVQKLAAQAAERARAAGIPIGTLAGTPEMATQVRAAGFDF
EECCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHCCCCE
VGLGSDLGLLVHAAQASLHALRTPDAAAVHSLHVGTHAY
EECCCCHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA