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The map label for this gene is cysW [H]

Identifier: 124265318

GI number: 124265318

Start: 127548

End: 128462

Strand: Direct

Name: cysW [H]

Synonym: Mpe_A0125

Alternate gene names: 124265318

Gene position: 127548-128462 (Clockwise)

Preceding gene: 124265317

Following gene: 124265319

Centisome position: 3.15

GC content: 67.1

Gene sequence:

>915_bases
ATGACGACCGGCGCCACGCTGGCCCTGAAGGCCGCTGCACCCGCTGCGGCGGTCGCTCCGACGAACCTGCGCAACGCCAC
CTCGGAGGCGCCCTGGGTGCGCCGCACGCTGATCGGCATCGCGCTGGTGTTCCTGACGGTGTTCCTGTTCGTGCCGCTGT
TCGCGGTGTTCTACGAGGCGCTGAAGAAGGGCTGGGATGTCTATCTCGCGGCGATCGTCGAGCCCGACGCGCTGTCGGCC
ATCCGGCTCACGCTGCTGGCGGCAGCGGTGTCGCTGCCGCTGAACCTGGTGTTCGGCGTGGCGGCGGCCTGGTGTATCGC
CAAGTTCAACTTCCGCGGCAAGAACATCCTGCTGACGCTGATCGACCTGCCGTTCTCGGTGAGCCCGGTGATCGCCGGCC
TGATCTTCGTGCTGGTGTTCGGCCTGCAGGGCTGGCTGGGCGGCTGGCTGCGCGAGCACGACCTGAAGATCATCTTCGCG
CTGCCTGGCATCGTGCTGGCCACGGTGTTCATCACCTTCCCGTTCATCGCGCGCGAGCTGATCCCGCTGATGCAGGCGCA
GGGCGTCGAGCAGGAAGAGGCGGCGCGCGTGCTGGGCGCTTCGGGCTGGCAGATCTTCTGGCGCGTGACGCTGCCCAACA
TCAAGTGGGCGCTGCTGTATGGCGTGATCCTGTGCAATGCGCGGGCGATGGGGGAGTTCGGCGCGGTGTCGGTGGTCTCG
GGCCACATCCGCGGCCAGACCAACACGATGCCGCTGCACATCGAGATCCTCTACAACGAATACCAGTTCGCGGCGTCGTT
CGCGGTGGCTTCGCTGCTGGCCGGGCTGGCGCTGGTGACGCTGGTGCTGAAGTACCTGGTGGAGCGACGGGTGAGAAACC
AGATCAAGGGCGCGAGCGCGCTGGAGGCCGTATGA

Upstream 100 bases:

>100_bases
ACCGCGATCGCGGTCGTGATGCTGGTGTCGAGCTTCGTGCTGCTGCTGGGCATCAACCTGCTGCAGGCCTGGTCGCGCCG
TCGCCAGGGAGGAAACTGAC

Downstream 100 bases:

>100_bases
GCATCGAAGTCCGTAACCTGAACAAGCGCTTCGGCCCCACCGTCGTCTGCGACGACATCTCGATCGACATCCCCTCGGGC
GAGCTGGTCGCGCTGCTCGG

Product: ABC type transporter

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTTGATLALKAAAPAAAVAPTNLRNATSEAPWVRRTLIGIALVFLTVFLFVPLFAVFYEALKKGWDVYLAAIVEPDALSA
IRLTLLAAAVSLPLNLVFGVAAAWCIAKFNFRGKNILLTLIDLPFSVSPVIAGLIFVLVFGLQGWLGGWLREHDLKIIFA
LPGIVLATVFITFPFIARELIPLMQAQGVEQEEAARVLGASGWQIFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVS
GHIRGQTNTMPLHIEILYNEYQFAASFAVASLLAGLALVTLVLKYLVERRVRNQIKGASALEAV

Sequences:

>Translated_304_residues
MTTGATLALKAAAPAAAVAPTNLRNATSEAPWVRRTLIGIALVFLTVFLFVPLFAVFYEALKKGWDVYLAAIVEPDALSA
IRLTLLAAAVSLPLNLVFGVAAAWCIAKFNFRGKNILLTLIDLPFSVSPVIAGLIFVLVFGLQGWLGGWLREHDLKIIFA
LPGIVLATVFITFPFIARELIPLMQAQGVEQEEAARVLGASGWQIFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVS
GHIRGQTNTMPLHIEILYNEYQFAASFAVASLLAGLALVTLVLKYLVERRVRNQIKGASALEAV
>Mature_303_residues
TTGATLALKAAAPAAAVAPTNLRNATSEAPWVRRTLIGIALVFLTVFLFVPLFAVFYEALKKGWDVYLAAIVEPDALSAI
RLTLLAAAVSLPLNLVFGVAAAWCIAKFNFRGKNILLTLIDLPFSVSPVIAGLIFVLVFGLQGWLGGWLREHDLKIIFAL
PGIVLATVFITFPFIARELIPLMQAQGVEQEEAARVLGASGWQIFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVSG
HIRGQTNTMPLHIEILYNEYQFAASFAVASLLAGLALVTLVLKYLVERRVRNQIKGASALEAV

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=271, Percent_Identity=52.7675276752767, Blast_Score=295, Evalue=2e-81,
Organism=Escherichia coli, GI1788764, Length=212, Percent_Identity=37.2641509433962, Blast_Score=154, Evalue=8e-39,
Organism=Escherichia coli, GI1786980, Length=227, Percent_Identity=29.0748898678414, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 32945; Mature: 32813

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS00656 GLYCOSYL_HYDROL_F6_2 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTGATLALKAAAPAAAVAPTNLRNATSEAPWVRRTLIGIALVFLTVFLFVPLFAVFYEA
CCCCCEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKGWDVYLAAIVEPDALSAIRLTLLAAAVSLPLNLVFGVAAAWCIAKFNFRGKNILLTL
HHCCHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
IDLPFSVSPVIAGLIFVLVFGLQGWLGGWLREHDLKIIFALPGIVLATVFITFPFIAREL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHH
IPLMQAQGVEQEEAARVLGASGWQIFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVS
HHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHEE
GHIRGQTNTMPLHIEILYNEYQFAASFAVASLLAGLALVTLVLKYLVERRVRNQIKGASA
CCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LEAV
HCCC
>Mature Secondary Structure 
TTGATLALKAAAPAAAVAPTNLRNATSEAPWVRRTLIGIALVFLTVFLFVPLFAVFYEA
CCCCEEEEECCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKKGWDVYLAAIVEPDALSAIRLTLLAAAVSLPLNLVFGVAAAWCIAKFNFRGKNILLTL
HHCCHHEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
IDLPFSVSPVIAGLIFVLVFGLQGWLGGWLREHDLKIIFALPGIVLATVFITFPFIAREL
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHH
IPLMQAQGVEQEEAARVLGASGWQIFWRVTLPNIKWALLYGVILCNARAMGEFGAVSVVS
HHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCHHHEE
GHIRGQTNTMPLHIEILYNEYQFAASFAVASLLAGLALVTLVLKYLVERRVRNQIKGASA
CCCCCCCCCCEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
LEAV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]