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The map label for this gene is thrS

Identifier: 124027839

GI number: 124027839

Start: 977314

End: 979191

Strand: Reverse

Name: thrS

Synonym: Hbut_0966

Alternate gene names: 124027839

Gene position: 979191-977314 (Counterclockwise)

Preceding gene: 124027841

Following gene: 124027838

Centisome position: 58.73

GC content: 49.79

Gene sequence:

>1878_bases
TTGAGAGTGCTACTAATACATGCTAAACGGTTCATCTACCGGACAAGGATGAAAGCTATACCTGAGGCAGAAGATATTAA
CGGCGTACAAGGAGAAGGCAGCTTCGAGAACGCCCTCGTCGTATTTACCACTGTTGAGAGCCGCGATACTAGCAACCCAA
AGGAAGTCGTAGAGACTGCTGCCAAGGATATCGTTGAGATAGCCTCAAAGGTTGGAGCTAAGACGATAGTCGTGTACCCC
TATGCCCATCTCTCACAAAAGCTTGCACCGCCTCATCAAGCCAAGAATATCTTAGCAATGCTCGAGTTGGAGATAAAGAA
GCTGGCTAGCGACAAGTTTGAGGTTAGCAGGGCACCATTTGGCTGGTATAAGGAGTTTGAGCTGCACTGCCACGGTCACC
CATTATCAGAGCTTTCAAGAAACTACGAGGCTAAGACCACGGCTAGGGTTGAGGTTAAGAAGAAGTACTACGTTCTAACC
CCGGAGGGCGAAGTCTATAGTCCCGAAGAGTTCCTTGAGAAGGCTTCTCCAGAGTTTAGGGCTGTAATAGAGAAGGAGGC
TCTCGGCAAGGAGATTGGCGGAGTTGAAAATCCAGTTAACAAGCTGTGCGCAAAATTCGGTTTTGAATGGGAGCCATTAT
CCGATTACGGACACATGCGCTACGAGCCACACGCTACACTAATGATAGAGGCTGTAGGCGAGTATGCATGGATACTTGCT
AGAAGCCTCACAATCCCCGTGCTAAAGGTTAAGGGTACAAACATGTTCGACCTGGCAGAAAAGCCCGTCTATGAACATGC
AGCACTCTTCGGCGACCGCCTCTATGAACTATGGGCTGATAAGAAACACCTCGTCATGAGATACGCGGCATGCCATCAAC
AGTTCTCTATGCTTAAAGACTATGTTCTCAGCTACCGGGACCTTCCGCTCGGAATGTTCGAGATTGCCGATAGCTACAGG
CTCGAGCAGAGTGGAGAGGTTACACTCTGCTTTAGGCTACGCAGATTCTATATGCCCGACCTTCACATACTGGCTCGCAG
CGTTGAAGAAGCGGTAAAGATTGCTGAGGAGCTGCAAAAGATTATACACCGGGAGGCTGAGAAGCTAGGCCAGACATACT
ACGCCGTATACAATGTAACCGAGGACTTCTGGGAGGAGAAACGCAACCTACTACTAGAACTCGTAAGGCGTGATGGGAAG
CCTGCCTTGATAACAGTCTATCCGGCAGGCATATACTACTGGGTAGTTAACGTCGAGTATCACATTGTGGATTCTGCTGG
CAGGCCCCGGGAGATAGCAACTTTCCAATTTGACGTCGGCAACGCGAAAAGGTTCGGAATAAGATATGTCGATGAGAATA
ACAAGGAGCATTACCCTGTGATAATACATACTGCACTCATAGGCTCCATTGAGAGATACATATACATGGTATTCGATGCC
GCTGTAAAGATGGAGCGGCGCGGCCAGACACCCTACATACCAACATGGCTGGCACCAATACAAGTAAGGCTAATCCCGGT
CAATCCGTCCTCAGAGCAACAGCTCAGCCATGCCGAGAAGGTTGCAAGTCTGCTGGAACGGCACCTTATAAGGGTCGATA
TTGATGATAGACAGTTAAGCCTGGGAAGGAGGATAAGGGATGCAGCTAGGGAGTGGATACCCTACATAGCGGTTATAGGC
GACCGCGAGGTGGAAACCGGCACCGTCAACGTAACCATACGACGCACCAACGATAGGGTAGCGGTGAAGCCCGAGGAGCT
GCTGGAAATGGTGCTAAAAGACCTTGAGGGATACCCGAGAGTACAGTCAACACTTCCACGCTACGTATCCAAGAGGCCCA
CGCTAGTATACCTTGAGAAGGAGGTTGCGCTGGAGTAG

Upstream 100 bases:

>100_bases
GGCCTTAGCTTAAAAGCACATGTCGACCCGCGAAACCCCTTAAATAATCCTCGCCGAGCTGTGATGTGCTGGGCTAATTG
CTTGGCCTAGGTGTAGCGTT

Downstream 100 bases:

>100_bases
ATGTTTTTGGGCTCAGTACGCTGAAGGCATTTCATTGCAACTGCTCGTAACAGGGGTTCTTTCATCACAGCCAGCCCGGT
ATCATATTATACCCTCTCAA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MRVLLIHAKRFIYRTRMKAIPEAEDINGVQGEGSFENALVVFTTVESRDTSNPKEVVETAAKDIVEIASKVGAKTIVVYP
YAHLSQKLAPPHQAKNILAMLELEIKKLASDKFEVSRAPFGWYKEFELHCHGHPLSELSRNYEAKTTARVEVKKKYYVLT
PEGEVYSPEEFLEKASPEFRAVIEKEALGKEIGGVENPVNKLCAKFGFEWEPLSDYGHMRYEPHATLMIEAVGEYAWILA
RSLTIPVLKVKGTNMFDLAEKPVYEHAALFGDRLYELWADKKHLVMRYAACHQQFSMLKDYVLSYRDLPLGMFEIADSYR
LEQSGEVTLCFRLRRFYMPDLHILARSVEEAVKIAEELQKIIHREAEKLGQTYYAVYNVTEDFWEEKRNLLLELVRRDGK
PALITVYPAGIYYWVVNVEYHIVDSAGRPREIATFQFDVGNAKRFGIRYVDENNKEHYPVIIHTALIGSIERYIYMVFDA
AVKMERRGQTPYIPTWLAPIQVRLIPVNPSSEQQLSHAEKVASLLERHLIRVDIDDRQLSLGRRIRDAAREWIPYIAVIG
DREVETGTVNVTIRRTNDRVAVKPEELLEMVLKDLEGYPRVQSTLPRYVSKRPTLVYLEKEVALE

Sequences:

>Translated_625_residues
MRVLLIHAKRFIYRTRMKAIPEAEDINGVQGEGSFENALVVFTTVESRDTSNPKEVVETAAKDIVEIASKVGAKTIVVYP
YAHLSQKLAPPHQAKNILAMLELEIKKLASDKFEVSRAPFGWYKEFELHCHGHPLSELSRNYEAKTTARVEVKKKYYVLT
PEGEVYSPEEFLEKASPEFRAVIEKEALGKEIGGVENPVNKLCAKFGFEWEPLSDYGHMRYEPHATLMIEAVGEYAWILA
RSLTIPVLKVKGTNMFDLAEKPVYEHAALFGDRLYELWADKKHLVMRYAACHQQFSMLKDYVLSYRDLPLGMFEIADSYR
LEQSGEVTLCFRLRRFYMPDLHILARSVEEAVKIAEELQKIIHREAEKLGQTYYAVYNVTEDFWEEKRNLLLELVRRDGK
PALITVYPAGIYYWVVNVEYHIVDSAGRPREIATFQFDVGNAKRFGIRYVDENNKEHYPVIIHTALIGSIERYIYMVFDA
AVKMERRGQTPYIPTWLAPIQVRLIPVNPSSEQQLSHAEKVASLLERHLIRVDIDDRQLSLGRRIRDAAREWIPYIAVIG
DREVETGTVNVTIRRTNDRVAVKPEELLEMVLKDLEGYPRVQSTLPRYVSKRPTLVYLEKEVALE
>Mature_625_residues
MRVLLIHAKRFIYRTRMKAIPEAEDINGVQGEGSFENALVVFTTVESRDTSNPKEVVETAAKDIVEIASKVGAKTIVVYP
YAHLSQKLAPPHQAKNILAMLELEIKKLASDKFEVSRAPFGWYKEFELHCHGHPLSELSRNYEAKTTARVEVKKKYYVLT
PEGEVYSPEEFLEKASPEFRAVIEKEALGKEIGGVENPVNKLCAKFGFEWEPLSDYGHMRYEPHATLMIEAVGEYAWILA
RSLTIPVLKVKGTNMFDLAEKPVYEHAALFGDRLYELWADKKHLVMRYAACHQQFSMLKDYVLSYRDLPLGMFEIADSYR
LEQSGEVTLCFRLRRFYMPDLHILARSVEEAVKIAEELQKIIHREAEKLGQTYYAVYNVTEDFWEEKRNLLLELVRRDGK
PALITVYPAGIYYWVVNVEYHIVDSAGRPREIATFQFDVGNAKRFGIRYVDENNKEHYPVIIHTALIGSIERYIYMVFDA
AVKMERRGQTPYIPTWLAPIQVRLIPVNPSSEQQLSHAEKVASLLERHLIRVDIDDRQLSLGRRIRDAAREWIPYIAVIG
DREVETGTVNVTIRRTNDRVAVKPEELLEMVLKDLEGYPRVQSTLPRYVSKRPTLVYLEKEVALE

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=343, Percent_Identity=26.2390670553936, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI38202255, Length=326, Percent_Identity=26.6871165644172, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI20070344, Length=321, Percent_Identity=27.4143302180685, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1788013, Length=385, Percent_Identity=25.4545454545455, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71984192, Length=351, Percent_Identity=24.2165242165242, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71984184, Length=351, Percent_Identity=24.2165242165242, Blast_Score=96, Evalue=8e-20,
Organism=Saccharomyces cerevisiae, GI6322654, Length=301, Percent_Identity=25.5813953488372, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322112, Length=351, Percent_Identity=23.0769230769231, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24583842, Length=338, Percent_Identity=26.0355029585799, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI28574141, Length=338, Percent_Identity=26.0355029585799, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24583839, Length=338, Percent_Identity=26.0355029585799, Blast_Score=112, Evalue=8e-25,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_HYPBU (A2BLF3)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013159.1
- ProteinModelPortal:   A2BLF3
- SMR:   A2BLF3
- STRING:   A2BLF3
- GeneID:   4781838
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0966
- eggNOG:   arNOG04477
- HOGENOM:   HBG392335
- OMA:   ETPVMYD
- ProtClustDB:   PRK03991
- BioCyc:   HBUT415426:HBUT_0966-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_A
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002320
- InterPro:   IPR015011
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01047
- ProDom:   PD016189

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF08915 tRNA-Thr_ED; SSF52954 Anticodon_bd

EC number: =6.1.1.3

Molecular weight: Translated: 72132; Mature: 72132

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLLIHAKRFIYRTRMKAIPEAEDINGVQGEGSFENALVVFTTVESRDTSNPKEVVETA
CEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
AKDIVEIASKVGAKTIVVYPYAHLSQKLAPPHQAKNILAMLELEIKKLASDKFEVSRAPF
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCC
GWYKEFELHCHGHPLSELSRNYEAKTTARVEVKKKYYVLTPEGEVYSPEEFLEKASPEFR
CCCEEEEEEECCCCHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHCCCHHH
AVIEKEALGKEIGGVENPVNKLCAKFGFEWEPLSDYGHMRYEPHATLMIEAVGEYAWILA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHH
RSLTIPVLKVKGTNMFDLAEKPVYEHAALFGDRLYELWADKKHLVMRYAACHQQFSMLKD
HHCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YVLSYRDLPLGMFEIADSYRLEQSGEVTLCFRLRRFYMPDLHILARSVEEAVKIAEELQK
HHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IIHREAEKLGQTYYAVYNVTEDFWEEKRNLLLELVRRDGKPALITVYPAGIYYWVVNVEY
HHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEE
HIVDSAGRPREIATFQFDVGNAKRFGIRYVDENNKEHYPVIIHTALIGSIERYIYMVFDA
EEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
AVKMERRGQTPYIPTWLAPIQVRLIPVNPSSEQQLSHAEKVASLLERHLIRVDIDDRQLS
HHHHHHCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEEECCCHHHH
LGRRIRDAAREWIPYIAVIGDREVETGTVNVTIRRTNDRVAVKPEELLEMVLKDLEGYPR
HHHHHHHHHHHHCCEEEEECCCCEECCEEEEEEEECCCEEEECHHHHHHHHHHHCCCCCH
VQSTLPRYVSKRPTLVYLEKEVALE
HHHHHHHHHCCCCCEEEEEHHHCCC
>Mature Secondary Structure
MRVLLIHAKRFIYRTRMKAIPEAEDINGVQGEGSFENALVVFTTVESRDTSNPKEVVETA
CEEEEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHH
AKDIVEIASKVGAKTIVVYPYAHLSQKLAPPHQAKNILAMLELEIKKLASDKFEVSRAPF
HHHHHHHHHHCCCCEEEEECCHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCC
GWYKEFELHCHGHPLSELSRNYEAKTTARVEVKKKYYVLTPEGEVYSPEEFLEKASPEFR
CCCEEEEEEECCCCHHHHHCCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHCCCHHH
AVIEKEALGKEIGGVENPVNKLCAKFGFEWEPLSDYGHMRYEPHATLMIEAVGEYAWILA
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHH
RSLTIPVLKVKGTNMFDLAEKPVYEHAALFGDRLYELWADKKHLVMRYAACHQQFSMLKD
HHCCEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
YVLSYRDLPLGMFEIADSYRLEQSGEVTLCFRLRRFYMPDLHILARSVEEAVKIAEELQK
HHHHHCCCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IIHREAEKLGQTYYAVYNVTEDFWEEKRNLLLELVRRDGKPALITVYPAGIYYWVVNVEY
HHHHHHHHHCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEEEEEEE
HIVDSAGRPREIATFQFDVGNAKRFGIRYVDENNKEHYPVIIHTALIGSIERYIYMVFDA
EEEECCCCCCEEEEEEEECCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
AVKMERRGQTPYIPTWLAPIQVRLIPVNPSSEQQLSHAEKVASLLERHLIRVDIDDRQLS
HHHHHHCCCCCCCCEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHCEEEEECCCHHHH
LGRRIRDAAREWIPYIAVIGDREVETGTVNVTIRRTNDRVAVKPEELLEMVLKDLEGYPR
HHHHHHHHHHHHCCEEEEECCCCEECCEEEEEEEECCCEEEECHHHHHHHHHHHCCCCCH
VQSTLPRYVSKRPTLVYLEKEVALE
HHHHHHHHHCCCCCEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA