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The map label for this gene is nadE

Identifier: 124027805

GI number: 124027805

Start: 939577

End: 940407

Strand: Reverse

Name: nadE

Synonym: Hbut_0932

Alternate gene names: 124027805

Gene position: 940407-939577 (Counterclockwise)

Preceding gene: 124027808

Following gene: 124027803

Centisome position: 56.41

GC content: 53.55

Gene sequence:

>831_bases
TTGAGGATTACTCTCAACGAACTTCTAGACCTGGACTATGAAGGTGTTGCCCGCTCCATAGAAGAATTCATCAAGGGCTA
CGTTGAGTCAAGTGGCGCTAAGGGTGTGGTTGTTGGTCTAAGTGGAGGCGTAGATTCTACCACCACATTGTACTTGCTGG
TTAGGGCTCTGGGTCCGGAGCGTGTCCTAGTACTAGTGATGCCGGATTCCGATGTGACGCCGGAGGAGGATGTCCACGAT
GCCGTAGGAATTGCTGAAAGGCTGGGAGTTCGCTACAAGCTGATAGATATCAAACCCATTGTGGCCAGCTATCTGGTTGC
CATGGGTGAGGCACCGGATCGGCGTAGTAAGGGGAATCTAAGGGCTAGGGTGAGGATGACACTGCTCTACCTATACGCAA
ACATGGAGGGTCTACTTGTAGCGGGAACTGGTGATCGCAGCGAGCTGCTGATAGGCTACTTTACAAAGTACGGTGATGGC
GCTGTCGACTTTCTGCCTATAGGCTGCCTCTACAAGTCTCAAGTTAGGAGGCTAGCCCTACATCTCGGTGTGCCAGAGAA
GATAGCTCTCAAGCCTAGTAGCCCACGTCTATGGCCTGGCCAACTGGCAGAGGATGAGCTAGGCATGAAGTATGAAGAGA
TAGATTTGATCCTCTACGCATTGTTTGACAAGGGGCTATCGCCCGAGGAAGCTGCAAAAGCTACCGGGCTCCCCATCGAG
AAGGTTAGGCGCGTACTAGAGCTACACCGGGCTAGCGAGCATAAGCGCAGCCTGCCGCCAGCACCTGATCCAGCCGCTAC
CGTGTGGAGGTTTAGGAGGAGGAAGGGCTAG

Upstream 100 bases:

>100_bases
ATAACGAGTTCGGGTATAAGGCGAGCTTTGCTAGCTAGTGTTGATGTAGCCTACACGTAAAAATCTCTGGGGCCTGGTAG
GGTTTGCGGGTGTGCTGGAC

Downstream 100 bases:

>100_bases
CCGAGGGCTTTTACCTCGCCGGTATACTGTCTCAGTGATGTCTCGAGCAGCTCTGATAGCTCGGCCAGCAGCTCGTTTAC
AACCTCTACCGGGCTCTTGT

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MRITLNELLDLDYEGVARSIEEFIKGYVESSGAKGVVVGLSGGVDSTTTLYLLVRALGPERVLVLVMPDSDVTPEEDVHD
AVGIAERLGVRYKLIDIKPIVASYLVAMGEAPDRRSKGNLRARVRMTLLYLYANMEGLLVAGTGDRSELLIGYFTKYGDG
AVDFLPIGCLYKSQVRRLALHLGVPEKIALKPSSPRLWPGQLAEDELGMKYEEIDLILYALFDKGLSPEEAAKATGLPIE
KVRRVLELHRASEHKRSLPPAPDPAATVWRFRRRKG

Sequences:

>Translated_276_residues
MRITLNELLDLDYEGVARSIEEFIKGYVESSGAKGVVVGLSGGVDSTTTLYLLVRALGPERVLVLVMPDSDVTPEEDVHD
AVGIAERLGVRYKLIDIKPIVASYLVAMGEAPDRRSKGNLRARVRMTLLYLYANMEGLLVAGTGDRSELLIGYFTKYGDG
AVDFLPIGCLYKSQVRRLALHLGVPEKIALKPSSPRLWPGQLAEDELGMKYEEIDLILYALFDKGLSPEEAAKATGLPIE
KVRRVLELHRASEHKRSLPPAPDPAATVWRFRRRKG
>Mature_276_residues
MRITLNELLDLDYEGVARSIEEFIKGYVESSGAKGVVVGLSGGVDSTTTLYLLVRALGPERVLVLVMPDSDVTPEEDVHD
AVGIAERLGVRYKLIDIKPIVASYLVAMGEAPDRRSKGNLRARVRMTLLYLYANMEGLLVAGTGDRSELLIGYFTKYGDG
AVDFLPIGCLYKSQVRRLALHLGVPEKIALKPSSPRLWPGQLAEDELGMKYEEIDLILYALFDKGLSPEEAAKATGLPIE
KVRRVLELHRASEHKRSLPPAPDPAATVWRFRRRKG

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Homo sapiens, GI41393551, Length=318, Percent_Identity=24.5283018867925, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1788036, Length=262, Percent_Identity=29.7709923664122, Blast_Score=85, Evalue=6e-18,
Organism=Drosophila melanogaster, GI24641841, Length=340, Percent_Identity=25.8823529411765, Blast_Score=75, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24641843, Length=340, Percent_Identity=25.8823529411765, Blast_Score=75, Evalue=7e-14,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_HYPBU (A2BLB9)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013125.1
- ProteinModelPortal:   A2BLB9
- SMR:   A2BLB9
- STRING:   A2BLB9
- GeneID:   4781885
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0932
- eggNOG:   arNOG08797
- HOGENOM:   HBG351567
- OMA:   PELEWAM
- PhylomeDB:   A2BLB9
- ProtClustDB:   PRK13980
- BioCyc:   HBUT415426:HBUT_0932-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30515; Mature: 30515

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: ACT_SITE 41-41

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITLNELLDLDYEGVARSIEEFIKGYVESSGAKGVVVGLSGGVDSTTTLYLLVRALGPE
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
RVLVLVMPDSDVTPEEDVHDAVGIAERLGVRYKLIDIKPIVASYLVAMGEAPDRRSKGNL
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCH
RARVRMTLLYLYANMEGLLVAGTGDRSELLIGYFTKYGDGAVDFLPIGCLYKSQVRRLAL
HHHHHHHHHHEEECCCEEEEECCCCCCCEEEEEHHHCCCCCEEEEEHHHHHHHHHHHHHH
HLGVPEKIALKPSSPRLWPGQLAEDELGMKYEEIDLILYALFDKGLSPEEAAKATGLPIE
HCCCCCEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
KVRRVLELHRASEHKRSLPPAPDPAATVWRFRRRKG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRITLNELLDLDYEGVARSIEEFIKGYVESSGAKGVVVGLSGGVDSTTTLYLLVRALGPE
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCC
RVLVLVMPDSDVTPEEDVHDAVGIAERLGVRYKLIDIKPIVASYLVAMGEAPDRRSKGNL
EEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECHHHHHHHHHHHCCCCCCCCCCCH
RARVRMTLLYLYANMEGLLVAGTGDRSELLIGYFTKYGDGAVDFLPIGCLYKSQVRRLAL
HHHHHHHHHHEEECCCEEEEECCCCCCCEEEEEHHHCCCCCEEEEEHHHHHHHHHHHHHH
HLGVPEKIALKPSSPRLWPGQLAEDELGMKYEEIDLILYALFDKGLSPEEAAKATGLPIE
HCCCCCEEEECCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCHH
KVRRVLELHRASEHKRSLPPAPDPAATVWRFRRRKG
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA