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The map label for this gene is eno [H]

Identifier: 124027784

GI number: 124027784

Start: 917453

End: 918796

Strand: Reverse

Name: eno [H]

Synonym: Hbut_0911

Alternate gene names: 124027784

Gene position: 918796-917453 (Counterclockwise)

Preceding gene: 124027789

Following gene: 124027783

Centisome position: 55.11

GC content: 49.78

Gene sequence:

>1344_bases
ATGAGCTTGCCCGTTGGCTACGATGATAGCTATATAATATCTCGCGTTAGGGCTAGGATGATCCTGGACTCTAGGGGCAA
TCCTACCGTTGAGGTTGAGGTTGTTACGCGTGGAGGAGGCTTCGGCAGGGCCGCTGCTCCTGCCGGCGCCTCGAAGGGCA
AGCATGAAGCTCTAGAGCTACGTGATGGTGGTAAAAAGTTTGGTGGCCGTGGTGTTGACCGTGCACTGTTTAATGTAAAT
CACGTGATCGCACCAAGAATTACGGGTCTTGACGCGAGGATGCAAAGGCTAATAGATAGTATTATGGTTGATCTTGACGG
CACACCCAACAAGTCAAGGCTTGGCGCCAATGCTATTGTCGCTACTTCGCTTGCTGTTGCAAAAGCTGCTGCTGATACCG
CTGGTATGCCCCTATACCGCTACCTCGGTGGTACCGGGACATTTGTGATGCCTGTTCCACTAATGAATATCATTAATGGC
GGTGCTCATGCTGGTAACGAGCTTTCATTCCAGGAGTTTATGATTGCACCCGTAGGCGCTGATAGCTTCAGCGAAGCTCT
AAGAATTGGCGTTGAAGTTTACCAGATGCTTAAGAAATATTTGAAGGATAAATATGGAGCTAGCGCCATTAACGTCGGTG
ATGAGGGAGGCTATGCACCACCCATGAAGTCTAACAAGGAGGCCCTTGACGCGCTTGTCGAGGCTATCAAGCTAGCTGGT
TACGAGCCTGGTGCAGATGTTGTGTTAGGAATTGATGTAGCAGCTTCACAATTCTACGATGCCGAGTCTGGAACCTATAG
TGTCGACGGACATAGACTTTCAGCCGAGGAGCTGCTTGATTACTATCTCGTCATGGTTGATGAGTATCCTATTAGGAGTA
TTGAGGATCCATTCTACGAGGAGGACTATGAGTCCTTTGCTAAGCTGACAAGTAAGCTTCTTGGCAGAGTTCTCATTGTA
GGGGATGACCTCTACGTGACTAATATTGGGAGGCTTGTACGTGGCATAGAGTTGAAAGCTACGACTGCAGCACTACTAAA
GGTTAACCAGGTGGGTACACTTACTGAGGCGCTAGACTATGCCTATACGGCCATGGCTAGCAATCTGAAGGTCATTGTTA
GTCATCGCAGTGGAGAGACTGAGGATACAACAATTGCGCATCTCGCGGTAGCGCTTAGGGCAGGGTTCATTAAGACTGGA
GCACCTGCTAGGGGCGAGAGGACCGCGAAGTATAACGAACTGTTGCGCATCGAGGAGGACTGTAGTGGAGAGTGCATCTA
TCCTGGTATTAGGGCTTACCAGTCGCTCCACCCGGTAACAGCAGCATACCAGCTACCTGCTTAG

Upstream 100 bases:

>100_bases
GAGCTTTTTGGCTAGGAGTGAGCCTAGTTTACCTGCGCCAACAATTAGTATGCGCATTGGATACTCTTCCCTCGGTTGGG
CTGAGGGGGTGGACTCTATT

Downstream 100 bases:

>100_bases
CACTACCCGGCAACTCTCTAGCAAGGGTTACCGCCATGCCTACACGTAGACTCATACTAATCGCGACAGCCAAAAAGTGG
AACCTTGCACTTGGCGCTGA

Product: enolase (2-phosphoglycerate dehydratase)

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSLPVGYDDSYIISRVRARMILDSRGNPTVEVEVVTRGGGFGRAAAPAGASKGKHEALELRDGGKKFGGRGVDRALFNVN
HVIAPRITGLDARMQRLIDSIMVDLDGTPNKSRLGANAIVATSLAVAKAAADTAGMPLYRYLGGTGTFVMPVPLMNIING
GAHAGNELSFQEFMIAPVGADSFSEALRIGVEVYQMLKKYLKDKYGASAINVGDEGGYAPPMKSNKEALDALVEAIKLAG
YEPGADVVLGIDVAASQFYDAESGTYSVDGHRLSAEELLDYYLVMVDEYPIRSIEDPFYEEDYESFAKLTSKLLGRVLIV
GDDLYVTNIGRLVRGIELKATTAALLKVNQVGTLTEALDYAYTAMASNLKVIVSHRSGETEDTTIAHLAVALRAGFIKTG
APARGERTAKYNELLRIEEDCSGECIYPGIRAYQSLHPVTAAYQLPA

Sequences:

>Translated_447_residues
MSLPVGYDDSYIISRVRARMILDSRGNPTVEVEVVTRGGGFGRAAAPAGASKGKHEALELRDGGKKFGGRGVDRALFNVN
HVIAPRITGLDARMQRLIDSIMVDLDGTPNKSRLGANAIVATSLAVAKAAADTAGMPLYRYLGGTGTFVMPVPLMNIING
GAHAGNELSFQEFMIAPVGADSFSEALRIGVEVYQMLKKYLKDKYGASAINVGDEGGYAPPMKSNKEALDALVEAIKLAG
YEPGADVVLGIDVAASQFYDAESGTYSVDGHRLSAEELLDYYLVMVDEYPIRSIEDPFYEEDYESFAKLTSKLLGRVLIV
GDDLYVTNIGRLVRGIELKATTAALLKVNQVGTLTEALDYAYTAMASNLKVIVSHRSGETEDTTIAHLAVALRAGFIKTG
APARGERTAKYNELLRIEEDCSGECIYPGIRAYQSLHPVTAAYQLPA
>Mature_446_residues
SLPVGYDDSYIISRVRARMILDSRGNPTVEVEVVTRGGGFGRAAAPAGASKGKHEALELRDGGKKFGGRGVDRALFNVNH
VIAPRITGLDARMQRLIDSIMVDLDGTPNKSRLGANAIVATSLAVAKAAADTAGMPLYRYLGGTGTFVMPVPLMNIINGG
AHAGNELSFQEFMIAPVGADSFSEALRIGVEVYQMLKKYLKDKYGASAINVGDEGGYAPPMKSNKEALDALVEAIKLAGY
EPGADVVLGIDVAASQFYDAESGTYSVDGHRLSAEELLDYYLVMVDEYPIRSIEDPFYEEDYESFAKLTSKLLGRVLIVG
DDLYVTNIGRLVRGIELKATTAALLKVNQVGTLTEALDYAYTAMASNLKVIVSHRSGETEDTTIAHLAVALRAGFIKTGA
PARGERTAKYNELLRIEEDCSGECIYPGIRAYQSLHPVTAAYQLPA

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the archaeal cel

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=50.9302325581395, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI4503571, Length=430, Percent_Identity=50.2325581395349, Blast_Score=400, Evalue=1e-111,
Organism=Homo sapiens, GI301897477, Length=430, Percent_Identity=50.2325581395349, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI301897469, Length=430, Percent_Identity=50.2325581395349, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI301897479, Length=427, Percent_Identity=47.3067915690867, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=25.6716417910448, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=25.6716417910448, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=25.6716417910448, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789141, Length=425, Percent_Identity=51.5294117647059, Blast_Score=379, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=51.2761020881671, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=51.2761020881671, Blast_Score=406, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI32563855, Length=191, Percent_Identity=45.0261780104712, Blast_Score=163, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324974, Length=437, Percent_Identity=50.5720823798627, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=437, Percent_Identity=50.5720823798627, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=437, Percent_Identity=50.3432494279176, Blast_Score=404, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=51.0392609699769, Blast_Score=397, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=51.5011547344111, Blast_Score=377, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=422, Percent_Identity=49.5260663507109, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580916, Length=422, Percent_Identity=49.5260663507109, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580920, Length=422, Percent_Identity=49.5260663507109, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580914, Length=422, Percent_Identity=49.5260663507109, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI281360527, Length=431, Percent_Identity=48.7238979118329, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI17137654, Length=431, Percent_Identity=48.7238979118329, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 48005; Mature: 47874

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPVGYDDSYIISRVRARMILDSRGNPTVEVEVVTRGGGFGRAAAPAGASKGKHEALEL
CCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
RDGGKKFGGRGVDRALFNVNHVIAPRITGLDARMQRLIDSIMVDLDGTPNKSRLGANAIV
CCCCHHCCCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHH
ATSLAVAKAAADTAGMPLYRYLGGTGTFVMPVPLMNIINGGAHAGNELSFQEFMIAPVGA
HHHHHHHHHHHCCCCCCHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHEEEECCCC
DSFSEALRIGVEVYQMLKKYLKDKYGASAINVGDEGGYAPPMKSNKEALDALVEAIKLAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YEPGADVVLGIDVAASQFYDAESGTYSVDGHRLSAEELLDYYLVMVDEYPIRSIEDPFYE
CCCCCCEEEEEEHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHH
EDYESFAKLTSKLLGRVLIVGDDLYVTNIGRLVRGIELKATTAALLKVNQVGTLTEALDY
HHHHHHHHHHHHHHCEEEEEECCEEEHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHH
AYTAMASNLKVIVSHRSGETEDTTIAHLAVALRAGFIKTGAPARGERTAKYNELLRIEED
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCC
CSGECIYPGIRAYQSLHPVTAAYQLPA
CCCCEECCHHHHHHHHCCCHHEEECCC
>Mature Secondary Structure 
SLPVGYDDSYIISRVRARMILDSRGNPTVEVEVVTRGGGFGRAAAPAGASKGKHEALEL
CCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
RDGGKKFGGRGVDRALFNVNHVIAPRITGLDARMQRLIDSIMVDLDGTPNKSRLGANAIV
CCCCHHCCCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCHHH
ATSLAVAKAAADTAGMPLYRYLGGTGTFVMPVPLMNIINGGAHAGNELSFQEFMIAPVGA
HHHHHHHHHHHCCCCCCHHHHHCCCCCEEEHHHHHHHHCCCCCCCCCCCHHHEEEECCCC
DSFSEALRIGVEVYQMLKKYLKDKYGASAINVGDEGGYAPPMKSNKEALDALVEAIKLAG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCC
YEPGADVVLGIDVAASQFYDAESGTYSVDGHRLSAEELLDYYLVMVDEYPIRSIEDPFYE
CCCCCCEEEEEEHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHH
EDYESFAKLTSKLLGRVLIVGDDLYVTNIGRLVRGIELKATTAALLKVNQVGTLTEALDY
HHHHHHHHHHHHHHCEEEEEECCEEEHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHH
AYTAMASNLKVIVSHRSGETEDTTIAHLAVALRAGFIKTGAPARGERTAKYNELLRIEED
HHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHEECCC
CSGECIYPGIRAYQSLHPVTAAYQLPA
CCCCEECCHHHHHHHHCCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA