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The map label for this gene is rnhB

Identifier: 124027720

GI number: 124027720

Start: 843255

End: 843977

Strand: Reverse

Name: rnhB

Synonym: Hbut_0844

Alternate gene names: 124027720

Gene position: 843977-843255 (Counterclockwise)

Preceding gene: 124027721

Following gene: 124027719

Centisome position: 50.62

GC content: 55.33

Gene sequence:

>723_bases
GTGCAACGGAGAAGATGCCGCTATGCTATCGGCATCGACGAGGCTGGTAGAGGGCCAGTTATAGGGCCAATGGTCGTTGT
AGGAGTTGCTGTGTGCAGCAATGATATCGACAAGCTTGTAGCACTAGGGGTTAGGGATAGTAAACAACTCACACCGGTTG
TCCGTGCAAAGCTCTACGGCGAGATTCTCCGCGTAGCACTACACAGTGTTATAGTGAAGCTGCCTCCAGCGCTCCTAGAT
GCTGTAAACCTAAACCAGCTAGAAGTCGAGACCTTCGAGTACATAGCGTCAAGGATAGCTGGTGTCCACGATAGCCCCGA
GGCAGTCTACGTCGATGCTGTGGGTAGCCCCGAGAAGCTGGCTGCAAGACTCTCTGGTAGGCTAGGCGTACGCGTCATAG
CCGAGCCGGGTGCTGATAAAACGTATCCCATAGTCTCTGCGGCGAGTATTGTTGCTAAGGTTGTGCGTGACGCGGAGATA
AGGATGCTACGCAGGCTCTACGGTGTAAGGGGCTCTGGCTATCCAACAGATCCCGAGACCATAGCATGGCTTGCAGAAGA
GTATAGGCGGAACCCTGCAAACCCGCCGTGGTTCGTACGGAGAACATGGTCCACACTGAAGCGTATAGCTCCGGGCTGGT
ACGTGGAGAAGCAGGCTACGACGCAACCTCCACGGGGGCAGAGGTCGCTCCTCGACTACCTCCTCGGAGAAAAGCAGAGC
TAG

Upstream 100 bases:

>100_bases
GGGGGTTGAGCTGGTCGACCCAGAGGAGGCAGCATGCATAGCATATGAGGGGCTAGCAGGCTATACTTGTGGCTAACAGT
TGGAAGGGGCGTGTAGAATG

Downstream 100 bases:

>100_bases
CCTGTAAAGATTCTGGGGTTGTGCCGGCTCCTCAGATACGGCCACACTCTTCACGGCCCCCTTTTGCGGGCTCTGGTAAG
CCCACCTATCATCACTGGCT

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MQRRRCRYAIGIDEAGRGPVIGPMVVVGVAVCSNDIDKLVALGVRDSKQLTPVVRAKLYGEILRVALHSVIVKLPPALLD
AVNLNQLEVETFEYIASRIAGVHDSPEAVYVDAVGSPEKLAARLSGRLGVRVIAEPGADKTYPIVSAASIVAKVVRDAEI
RMLRRLYGVRGSGYPTDPETIAWLAEEYRRNPANPPWFVRRTWSTLKRIAPGWYVEKQATTQPPRGQRSLLDYLLGEKQS

Sequences:

>Translated_240_residues
MQRRRCRYAIGIDEAGRGPVIGPMVVVGVAVCSNDIDKLVALGVRDSKQLTPVVRAKLYGEILRVALHSVIVKLPPALLD
AVNLNQLEVETFEYIASRIAGVHDSPEAVYVDAVGSPEKLAARLSGRLGVRVIAEPGADKTYPIVSAASIVAKVVRDAEI
RMLRRLYGVRGSGYPTDPETIAWLAEEYRRNPANPPWFVRRTWSTLKRIAPGWYVEKQATTQPPRGQRSLLDYLLGEKQS
>Mature_240_residues
MQRRRCRYAIGIDEAGRGPVIGPMVVVGVAVCSNDIDKLVALGVRDSKQLTPVVRAKLYGEILRVALHSVIVKLPPALLD
AVNLNQLEVETFEYIASRIAGVHDSPEAVYVDAVGSPEKLAARLSGRLGVRVIAEPGADKTYPIVSAASIVAKVVRDAEI
RMLRRLYGVRGSGYPTDPETIAWLAEEYRRNPANPPWFVRRTWSTLKRIAPGWYVEKQATTQPPRGQRSLLDYLLGEKQS

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Homo sapiens, GI38455391, Length=254, Percent_Identity=35.0393700787402, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786380, Length=202, Percent_Identity=32.1782178217822, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536263, Length=248, Percent_Identity=30.6451612903226, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324257, Length=221, Percent_Identity=32.1266968325792, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24580635, Length=216, Percent_Identity=35.6481481481481, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_HYPBU (A2BL34)

Other databases:

- EMBL:   CP000493
- RefSeq:   YP_001013040.1
- ProteinModelPortal:   A2BL34
- SMR:   A2BL34
- STRING:   A2BL34
- GeneID:   4782909
- GenomeReviews:   CP000493_GR
- KEGG:   hbu:Hbut_0844
- eggNOG:   arNOG07713
- HOGENOM:   HBG584843
- OMA:   AEHKADE
- PhylomeDB:   A2BL34
- ProtClustDB:   PRK00015
- BioCyc:   HBUT415426:HBUT_0844-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_A
- InterPro:   IPR004649
- InterPro:   IPR001352
- InterPro:   IPR020787
- InterPro:   IPR023160
- InterPro:   IPR012337
- Gene3D:   G3DSA:1.10.10.460
- PANTHER:   PTHR10954
- TIGRFAMs:   TIGR00729

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 26481; Mature: 26481

Theoretical pI: Translated: 10.03; Mature: 10.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRRCRYAIGIDEAGRGPVIGPMVVVGVAVCSNDIDKLVALGVRDSKQLTPVVRAKLYG
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EILRVALHSVIVKLPPALLDAVNLNQLEVETFEYIASRIAGVHDSPEAVYVDAVGSPEKL
HHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
AARLSGRLGVRVIAEPGADKTYPIVSAASIVAKVVRDAEIRMLRRLYGVRGSGYPTDPET
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IAWLAEEYRRNPANPPWFVRRTWSTLKRIAPGWYVEKQATTQPPRGQRSLLDYLLGEKQS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MQRRRCRYAIGIDEAGRGPVIGPMVVVGVAVCSNDIDKLVALGVRDSKQLTPVVRAKLYG
CCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
EILRVALHSVIVKLPPALLDAVNLNQLEVETFEYIASRIAGVHDSPEAVYVDAVGSPEKL
HHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHH
AARLSGRLGVRVIAEPGADKTYPIVSAASIVAKVVRDAEIRMLRRLYGVRGSGYPTDPET
HHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
IAWLAEEYRRNPANPPWFVRRTWSTLKRIAPGWYVEKQATTQPPRGQRSLLDYLLGEKQS
HHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA