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The map label for this gene is lplA [H]

Identifier: 124027679

GI number: 124027679

Start: 796036

End: 796809

Strand: Reverse

Name: lplA [H]

Synonym: Hbut_0801

Alternate gene names: 124027679

Gene position: 796809-796036 (Counterclockwise)

Preceding gene: 124027681

Following gene: 124027678

Centisome position: 47.79

GC content: 59.3

Gene sequence:

>774_bases
GTGCGGGGGACGCTGCGGGTCATATTGGACCTAGAGGGTGCTCCAGCAGTCTGGCAGATGGCGGTGGACGAGGCGCTGCT
TAGGCTGCGGAGTATGGGAAGGATACCCGACACGCTCCGCATTTACCGTTTCCGCCCCCACGCGGTAACCATTGGACGGT
TTCAGAGGCTAGAGTCTAGCGTAGACCTGGAGGAGGCTAGGAAGCTAGGCATAGACGTTGTTCGCAGGTTTACTGGGGGC
GGAAGCGTTTACCATGACACCGATGGAGAGGTCACCTACTCCATAGTCCTGGGGCTTGAGGGCCGTCCAGAGCTGCGTGA
TGTAGCCATGAGCTACCGTGTGCTCTGTCGCGGCGTTGTAGAGGCACTTCGCATTCTCGGTGTGGAGGCAGAGTACAAGC
CTATCAACGACGTGGTGGCTGGTGGCAGAAAGGTTTCGGGCAATGCGCAGGCTAGGACGGCCACAGCGGTGTTACAGCAT
GGCACCGTGCTGTACGCCACGAACCTTGACGTGATGGAGAGGGTCCTCCGCGTCCCTAGGGCAAAGCTCGAAAGCCATGG
AGCATTAAGGATAAGGGACCGCGTAGCAACGCTCTCCCAGCTTCTTGGCCGCGAACTCCACTACTGGGAGGTGGCGGTAG
CCCTCGTGGAGGGGTTCCGTATAGCACTGGGTTACGATGCAATACGTATTGACGCGTTGTCCGGCGAGGAGAAAAGGTTG
GCAGAACAGCTGGCCCCCAGGTATGCTAGCAGGGAGTGGCTCTACAGGAGGTAG

Upstream 100 bases:

>100_bases
CTAAACCCTATCGTAGTCGGCACAACGCGTGTTTAAATTGTGCACGGCAGACTGTGGCCTAATAGGTGGCCTGGCATTGC
ATGCCCTCGGGGGATGATTT

Downstream 100 bases:

>100_bases
GGAGGGACGGCCGGAGGCCTTGCCGTGTAACCGTTGTCCACTATCGTGCAGCGGTGGGAGCAGCTGCGGCGTGGTGGATG
CATCCAGGCAAGGCGACCTC

Product: lipoate-protein ligase A

Products: NA

Alternate protein names: Lipoate--protein ligase subunit 1 [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MRGTLRVILDLEGAPAVWQMAVDEALLRLRSMGRIPDTLRIYRFRPHAVTIGRFQRLESSVDLEEARKLGIDVVRRFTGG
GSVYHDTDGEVTYSIVLGLEGRPELRDVAMSYRVLCRGVVEALRILGVEAEYKPINDVVAGGRKVSGNAQARTATAVLQH
GTVLYATNLDVMERVLRVPRAKLESHGALRIRDRVATLSQLLGRELHYWEVAVALVEGFRIALGYDAIRIDALSGEEKRL
AEQLAPRYASREWLYRR

Sequences:

>Translated_257_residues
MRGTLRVILDLEGAPAVWQMAVDEALLRLRSMGRIPDTLRIYRFRPHAVTIGRFQRLESSVDLEEARKLGIDVVRRFTGG
GSVYHDTDGEVTYSIVLGLEGRPELRDVAMSYRVLCRGVVEALRILGVEAEYKPINDVVAGGRKVSGNAQARTATAVLQH
GTVLYATNLDVMERVLRVPRAKLESHGALRIRDRVATLSQLLGRELHYWEVAVALVEGFRIALGYDAIRIDALSGEEKRL
AEQLAPRYASREWLYRR
>Mature_257_residues
MRGTLRVILDLEGAPAVWQMAVDEALLRLRSMGRIPDTLRIYRFRPHAVTIGRFQRLESSVDLEEARKLGIDVVRRFTGG
GSVYHDTDGEVTYSIVLGLEGRPELRDVAMSYRVLCRGVVEALRILGVEAEYKPINDVVAGGRKVSGNAQARTATAVLQH
GTVLYATNLDVMERVLRVPRAKLESHGALRIRDRVATLSQLLGRELHYWEVAVALVEGFRIALGYDAIRIDALSGEEKRL
AEQLAPRYASREWLYRR

Specific function: Lipoate-protein ligase catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. This subunit can alone only catalyze the lipoat

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Escherichia coli, GI1790846, Length=263, Percent_Identity=30.0380228136882, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 28892; Mature: 28892

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGTLRVILDLEGAPAVWQMAVDEALLRLRSMGRIPDTLRIYRFRPHAVTIGRFQRLESS
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHHHHC
VDLEEARKLGIDVVRRFTGGGSVYHDTDGEVTYSIVLGLEGRPELRDVAMSYRVLCRGVV
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EALRILGVEAEYKPINDVVAGGRKVSGNAQARTATAVLQHGTVLYATNLDVMERVLRVPR
HHHHHHCCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHCCH
AKLESHGALRIRDRVATLSQLLGRELHYWEVAVALVEGFRIALGYDAIRIDALSGEEKRL
HHHHCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCEEEEEECCCHHHHH
AEQLAPRYASREWLYRR
HHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MRGTLRVILDLEGAPAVWQMAVDEALLRLRSMGRIPDTLRIYRFRPHAVTIGRFQRLESS
CCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEHHHHHHHHHC
VDLEEARKLGIDVVRRFTGGGSVYHDTDGEVTYSIVLGLEGRPELRDVAMSYRVLCRGVV
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EALRILGVEAEYKPINDVVAGGRKVSGNAQARTATAVLQHGTVLYATNLDVMERVLRVPR
HHHHHHCCCCCCCCHHHHHCCCEEECCCCHHHHHHHHHHCCCEEEEECHHHHHHHHHCCH
AKLESHGALRIRDRVATLSQLLGRELHYWEVAVALVEGFRIALGYDAIRIDALSGEEKRL
HHHHCCCCEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHEEECCCEEEEEECCCHHHHH
AEQLAPRYASREWLYRR
HHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029001 [H]