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The map label for this gene is dacB [C]

Identifier: 124025705

GI number: 124025705

Start: 919287

End: 920522

Strand: Direct

Name: dacB [C]

Synonym: NATL1_09981

Alternate gene names: 124025705

Gene position: 919287-920522 (Clockwise)

Preceding gene: 124025704

Following gene: 124025707

Centisome position: 49.3

GC content: 27.75

Gene sequence:

>1236_bases
ATGAAAAAAAAACTTTCTTTATATATTGGATTAATAATTATATCGATAAATACAGCTAATGGTAATTTGTTTTCGATATA
TTCCTACGTCCGACAAAGAATTAATATTAATTCTATAACTCCTGAAGAGACAAATATATGTTATGCATTACAAAAAAAGA
TAGAGGATTATATTGATAACTTTAATAGTAAAATAAGTGTTAATATATTAAATAAAAATGGTGAGTTTATTGTAGATATA
AATGGAAAAATTCCTAGAATTCCTGCATCGAATCAAAAGATTTTGTCGAGCGCTTTTTCTTTAGATAATTTAGGACCAAA
CTATATTCTCAAAACATCTCTAAATGAGATGAGTAATGGAGGCTTATACCTTGATGCCTCTGGTGATCCTGACTTTGATA
AAAGACATTTAGATGAGTTACTAAGAGGACTAAATAGTAAGGAGTTTAATACTGGTATTAAATTACCAATTTTAATCACT
AGTAGTAAAGAAAATACTTGGTGGCCTCCTAGCTGGTCCTATTCTGATAGGAAAGAGGAGTATGGAGCACCAATTACTAA
GTACTCGATAGCTAGTAATGCAAGTACAAATGCTTTAAACAATCCAATAGATAATTTTATGAATGAGTTAAAGACTTCTC
TAGTAAGAAATAACTTAGCAAGTAAATATTTTATTAAACCCGTTTATAATGATTTTTCTATCGATTTTGTTTCTACTATC
AAAAGTGTTAATTCTGCGCCCTTATATATTTTGCTTAATCTAGTTAATTCAGAAAGCCATAATTATACAGCTGAGGTTGT
TTTTAGACATTCATTAAATAACTGGTCTCATGATTTTCCTAATGTTAAATACACTAAGTGGATAAATGATCAGAACTTTG
ATTCAGATGATTTTATCTTTGCTGATGCTAGTGGTTTATCAAGAGAAAATAGAGTTACCACATATGGTTTATCTCAATTT
TTGAGAAGAATGCAATTAAGTAGATATTCCGATTATTATTTCTCATCTTTTTCTATTTTAGGGGTTAGAGGATCATTGAA
AAATGTAAAAGCCCCAATAAATCTCAAAGGGAGAATTTTGGCTAAATCAGGAAGACTTAGTAACGTTAGATCTATTTCTG
GTATTATATTAGGTAAAGATAAAATATTTAGTATTATTGTTAATAATAAGGAAAATTCGACGAAGAATATTATTAATATT
TTATCAATAGTAGATAATACTAATTATTGTACTTAA

Upstream 100 bases:

>100_bases
TGATTGGGAACTTTTAAATTAAATAATAATGGATATTCTTTTGTTAAATCATTTATTAATAGACCTGACGATGAAACAAT
AATTCTTAACTTGTGAATAT

Downstream 100 bases:

>100_bases
TTAGTATTTAGTTTCATAAGATCCTTAGCAATAAATTCAGGAACCATATGGCGAATCTCACCGCCAAATCTTGCGACTTC
TTTAACTACTGAACTACTTA

Product: D-Ala-D-Ala carboxypeptidase 3

Products: NA

Alternate protein names: Serine-Type D-Ala-D-Ala Carboxypeptidase; Peptidase; D-Ala-D-Ala Carboxypeptidase 3 Family Protein

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MKKKLSLYIGLIIISINTANGNLFSIYSYVRQRININSITPEETNICYALQKKIEDYIDNFNSKISVNILNKNGEFIVDI
NGKIPRIPASNQKILSSAFSLDNLGPNYILKTSLNEMSNGGLYLDASGDPDFDKRHLDELLRGLNSKEFNTGIKLPILIT
SSKENTWWPPSWSYSDRKEEYGAPITKYSIASNASTNALNNPIDNFMNELKTSLVRNNLASKYFIKPVYNDFSIDFVSTI
KSVNSAPLYILLNLVNSESHNYTAEVVFRHSLNNWSHDFPNVKYTKWINDQNFDSDDFIFADASGLSRENRVTTYGLSQF
LRRMQLSRYSDYYFSSFSILGVRGSLKNVKAPINLKGRILAKSGRLSNVRSISGIILGKDKIFSIIVNNKENSTKNIINI
LSIVDNTNYCT

Sequences:

>Translated_411_residues
MKKKLSLYIGLIIISINTANGNLFSIYSYVRQRININSITPEETNICYALQKKIEDYIDNFNSKISVNILNKNGEFIVDI
NGKIPRIPASNQKILSSAFSLDNLGPNYILKTSLNEMSNGGLYLDASGDPDFDKRHLDELLRGLNSKEFNTGIKLPILIT
SSKENTWWPPSWSYSDRKEEYGAPITKYSIASNASTNALNNPIDNFMNELKTSLVRNNLASKYFIKPVYNDFSIDFVSTI
KSVNSAPLYILLNLVNSESHNYTAEVVFRHSLNNWSHDFPNVKYTKWINDQNFDSDDFIFADASGLSRENRVTTYGLSQF
LRRMQLSRYSDYYFSSFSILGVRGSLKNVKAPINLKGRILAKSGRLSNVRSISGIILGKDKIFSIIVNNKENSTKNIINI
LSIVDNTNYCT
>Mature_411_residues
MKKKLSLYIGLIIISINTANGNLFSIYSYVRQRININSITPEETNICYALQKKIEDYIDNFNSKISVNILNKNGEFIVDI
NGKIPRIPASNQKILSSAFSLDNLGPNYILKTSLNEMSNGGLYLDASGDPDFDKRHLDELLRGLNSKEFNTGIKLPILIT
SSKENTWWPPSWSYSDRKEEYGAPITKYSIASNASTNALNNPIDNFMNELKTSLVRNNLASKYFIKPVYNDFSIDFVSTI
KSVNSAPLYILLNLVNSESHNYTAEVVFRHSLNNWSHDFPNVKYTKWINDQNFDSDDFIFADASGLSRENRVTTYGLSQF
LRRMQLSRYSDYYFSSFSILGVRGSLKNVKAPINLKGRILAKSGRLSNVRSISGIILGKDKIFSIIVNNKENSTKNIINI
LSIVDNTNYCT

Specific function: Not Involved In Transpeptidation But Exclusively Catalyzes A Dd-Carboxypeptidase And Dd-Endopeptidase Reaction. [C]

COG id: COG2027

COG function: function code M; D-alanyl-D-alanine carboxypeptidase (penicillin-binding protein 4)

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.4.16.4; 3.4.99.- [C]

Molecular weight: Translated: 46563; Mature: 46563

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKLSLYIGLIIISINTANGNLFSIYSYVRQRININSITPEETNICYALQKKIEDYIDN
CCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
FNSKISVNILNKNGEFIVDINGKIPRIPASNQKILSSAFSLDNLGPNYILKTSLNEMSNG
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHCCCC
GLYLDASGDPDFDKRHLDELLRGLNSKEFNTGIKLPILITSSKENTWWPPSWSYSDRKEE
CEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
YGAPITKYSIASNASTNALNNPIDNFMNELKTSLVRNNLASKYFIKPVYNDFSIDFVSTI
HCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
KSVNSAPLYILLNLVNSESHNYTAEVVFRHSLNNWSHDFPNVKYTKWINDQNFDSDDFIF
HHCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEE
ADASGLSRENRVTTYGLSQFLRRMQLSRYSDYYFSSFSILGVRGSLKNVKAPINLKGRIL
EECCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCHHCCCCCCCCCEEEE
AKSGRLSNVRSISGIILGKDKIFSIIVNNKENSTKNIINILSIVDNTNYCT
ECCCCCCCHHHHCEEEEECCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKKLSLYIGLIIISINTANGNLFSIYSYVRQRININSITPEETNICYALQKKIEDYIDN
CCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHC
FNSKISVNILNKNGEFIVDINGKIPRIPASNQKILSSAFSLDNLGPNYILKTSLNEMSNG
CCCEEEEEEEECCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEECHHHCCCC
GLYLDASGDPDFDKRHLDELLRGLNSKEFNTGIKLPILITSSKENTWWPPSWSYSDRKEE
CEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHH
YGAPITKYSIASNASTNALNNPIDNFMNELKTSLVRNNLASKYFIKPVYNDFSIDFVSTI
HCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
KSVNSAPLYILLNLVNSESHNYTAEVVFRHSLNNWSHDFPNVKYTKWINDQNFDSDDFIF
HHCCCCCEEEEEEECCCCCCCEEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEE
ADASGLSRENRVTTYGLSQFLRRMQLSRYSDYYFSSFSILGVRGSLKNVKAPINLKGRIL
EECCCCCCCCCEEHHHHHHHHHHHHHHHCCCHHCCCEEEEEECCCHHCCCCCCCCCEEEE
AKSGRLSNVRSISGIILGKDKIFSIIVNNKENSTKNIINILSIVDNTNYCT
ECCCCCCCHHHHCEEEEECCEEEEEEEECCCCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: Preferential cleavage is (Ac)2-L-Lys-D-Ala D-Ala and Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine [C]

General reaction: Hydrolase; Acting on peptide bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA