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The map label for this gene is dnaG [H]

Identifier: 124025647

GI number: 124025647

Start: 867908

End: 869785

Strand: Direct

Name: dnaG [H]

Synonym: NATL1_09401

Alternate gene names: 124025647

Gene position: 867908-869785 (Clockwise)

Preceding gene: 124025641

Following gene: 124025648

Centisome position: 46.54

GC content: 35.41

Gene sequence:

>1878_bases
ATGTCAGTAATTCCTGGCAAGCAATTTTATTATTGTTTTTCATGTGGAGCTGGTGGAAATGCAATTAAATTCTTAATGGA
GTTTCAGAGACAAAGTTTTAGTGATGTTGTTCTTGAGCTGGCTAAGAAATATCAAGTACCAATTGATACGATTGAAGGAC
CTCAACAAGAAAGACTCAAGCAACAGCTTTCACGTAGAGATACCCTTTATCGTGTTTTAAAAACCGCTACTGGTTGGTTT
AGAAATCAATTGAATTCTCCATGTGGAGAAAATGCACTTAATTACCTTAAGAATAAGCGTAATTTAAGTGATGGAACTTT
AATCAATTTTGAACTTGGTTTTGCTCCAGATAATTGGGATTCACTACTTAAATATTTTGTAGACATAGAAAAAGTCAGTG
TTGAAATCCTTGAATCGGCGGGATTGATTGTTCCTCGAAAGGGTGGTAATGGTTTCTATGACAGATTTCGCAATCGAATA
ATTGTTCCTATTCACGACAGGCAAAAAAGAGTTATTGGTTTCGGCGGACGAAGTCTTGATGGTTCAGAACCTAAGTATTT
AAATTCACCTGAGACTGAAATCTTTGAAAAAGGAAAAAATCTTTTTGGTTTTGATAAATCCACACTTTCCATTAGGAAAA
AAGATTATGCAGTTGTTGTAGAGGGATATTTTGATGTGATGGCACTTCATGATTCGGGTATTACAAATGTTGTCGCTTCT
TTAGGAACAGCTTTAAGTCGCAATCAAATAACGCTTCTTTCTCGTGCCACCGATAGTAAAAAGATCCTCTTAAATTTTGA
CTCAGATAATGCTGGAATTCGTGCGGCTAATAGAGCCATTAGTGAAGTAGAAAACCTTGCTATTCAAGGTCAACTAGATT
TACGAGTCCTTCAATTACCTTCAGGTAAAGATCCAGATGAATTTCTTAAGGGTAATTCTCCATCCGAATATGAAGCATTA
GCGGCAAGATCACCTCTTTGGATGGATTGGCAAATTGATCAATCATTGAAGGATTTAGATTTAAGTAAATCTGATCAATT
TCAGGAAGCCGTTAGCAGCTTAGTAAGTCTCCTTGGAAAGCTTCCTCAAACTGCAATAAGAACTCATTATCTACAGAAGG
TTGCTCAGCGTCTTAGTGGAGGTCAAGGTAGATTCGCTCTACAACTAGAGGAGGATTTACGTAATCAAATAAGTGGTCAA
AGATGGCACGGTCGCTCGAAAAAAATTGATAAGCCTCAAGAAATTAGTCTCAGAGAAAGAAGTGAGTCAGATATACTTTT
TACTTATATTCACTGTCCTAATTACAGATCTTTTATTCGTTATGAACTTCGCTTAAGGGATCTTGATGATTTTGCGATTA
ATCATCATCGTGCAATATGGTCTACAATAAGTAACATTGAGGAAAATATGTTTGGTCCAGAAACTGTTGAGAAGATTAAT
CGTTTTAATGATTCTAATAATATTTTAGCTGATGTTGATTTAATTAAAAAGTTGTTAGACAATTTCCTATCCAGTGATAA
TGAGCATCTTCCTAAACTTACTCCTTTACTAGATGTTAATGAACTTCGTTTGGCAACATTAAATGACCCCGAGTCGTTCA
TCCGTGGAGCTATGGCTGCTCTTGAAAAGCAAAAATCCTTAAAACGTTGTAGACATTTAATTGATGCATGGAGTTCACAG
AGATTGCAAACTCTTGAGAACTGTATAGCCTCTCTTATTGTTCAGGAAAAATCTGAGCCTAGCGATTCATCTGATATGGA
ACAGAGGGTTATTAGCATGTTTGAAGACTTAAATAATGATGCTATAAATTTTCAACAACTTTATTACGCTGAAAGAAAAC
ACATACTAAATCTAGATCAACAGAGATGTTATAAATAA

Upstream 100 bases:

>100_bases
GCGTAGATATTGTTGATGTTGTTGGTGAACACGTCGTCTTAAAGAAAAAGGTAAAGAATATGTTGGAATATGTCCTTTTC
ATGATGATAGTAAACCTTCA

Downstream 100 bases:

>100_bases
TTTTATATAGTTTTTACAATGAAAAATATCTCACCTTTTAATTTGGATCAATGTTCTTTATCTAATGATCTATGGCCAGA
TTTTATCAATAAAATTGGTC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MSVIPGKQFYYCFSCGAGGNAIKFLMEFQRQSFSDVVLELAKKYQVPIDTIEGPQQERLKQQLSRRDTLYRVLKTATGWF
RNQLNSPCGENALNYLKNKRNLSDGTLINFELGFAPDNWDSLLKYFVDIEKVSVEILESAGLIVPRKGGNGFYDRFRNRI
IVPIHDRQKRVIGFGGRSLDGSEPKYLNSPETEIFEKGKNLFGFDKSTLSIRKKDYAVVVEGYFDVMALHDSGITNVVAS
LGTALSRNQITLLSRATDSKKILLNFDSDNAGIRAANRAISEVENLAIQGQLDLRVLQLPSGKDPDEFLKGNSPSEYEAL
AARSPLWMDWQIDQSLKDLDLSKSDQFQEAVSSLVSLLGKLPQTAIRTHYLQKVAQRLSGGQGRFALQLEEDLRNQISGQ
RWHGRSKKIDKPQEISLRERSESDILFTYIHCPNYRSFIRYELRLRDLDDFAINHHRAIWSTISNIEENMFGPETVEKIN
RFNDSNNILADVDLIKKLLDNFLSSDNEHLPKLTPLLDVNELRLATLNDPESFIRGAMAALEKQKSLKRCRHLIDAWSSQ
RLQTLENCIASLIVQEKSEPSDSSDMEQRVISMFEDLNNDAINFQQLYYAERKHILNLDQQRCYK

Sequences:

>Translated_625_residues
MSVIPGKQFYYCFSCGAGGNAIKFLMEFQRQSFSDVVLELAKKYQVPIDTIEGPQQERLKQQLSRRDTLYRVLKTATGWF
RNQLNSPCGENALNYLKNKRNLSDGTLINFELGFAPDNWDSLLKYFVDIEKVSVEILESAGLIVPRKGGNGFYDRFRNRI
IVPIHDRQKRVIGFGGRSLDGSEPKYLNSPETEIFEKGKNLFGFDKSTLSIRKKDYAVVVEGYFDVMALHDSGITNVVAS
LGTALSRNQITLLSRATDSKKILLNFDSDNAGIRAANRAISEVENLAIQGQLDLRVLQLPSGKDPDEFLKGNSPSEYEAL
AARSPLWMDWQIDQSLKDLDLSKSDQFQEAVSSLVSLLGKLPQTAIRTHYLQKVAQRLSGGQGRFALQLEEDLRNQISGQ
RWHGRSKKIDKPQEISLRERSESDILFTYIHCPNYRSFIRYELRLRDLDDFAINHHRAIWSTISNIEENMFGPETVEKIN
RFNDSNNILADVDLIKKLLDNFLSSDNEHLPKLTPLLDVNELRLATLNDPESFIRGAMAALEKQKSLKRCRHLIDAWSSQ
RLQTLENCIASLIVQEKSEPSDSSDMEQRVISMFEDLNNDAINFQQLYYAERKHILNLDQQRCYK
>Mature_624_residues
SVIPGKQFYYCFSCGAGGNAIKFLMEFQRQSFSDVVLELAKKYQVPIDTIEGPQQERLKQQLSRRDTLYRVLKTATGWFR
NQLNSPCGENALNYLKNKRNLSDGTLINFELGFAPDNWDSLLKYFVDIEKVSVEILESAGLIVPRKGGNGFYDRFRNRII
VPIHDRQKRVIGFGGRSLDGSEPKYLNSPETEIFEKGKNLFGFDKSTLSIRKKDYAVVVEGYFDVMALHDSGITNVVASL
GTALSRNQITLLSRATDSKKILLNFDSDNAGIRAANRAISEVENLAIQGQLDLRVLQLPSGKDPDEFLKGNSPSEYEALA
ARSPLWMDWQIDQSLKDLDLSKSDQFQEAVSSLVSLLGKLPQTAIRTHYLQKVAQRLSGGQGRFALQLEEDLRNQISGQR
WHGRSKKIDKPQEISLRERSESDILFTYIHCPNYRSFIRYELRLRDLDDFAINHHRAIWSTISNIEENMFGPETVEKINR
FNDSNNILADVDLIKKLLDNFLSSDNEHLPKLTPLLDVNELRLATLNDPESFIRGAMAALEKQKSLKRCRHLIDAWSSQR
LQTLENCIASLIVQEKSEPSDSSDMEQRVISMFEDLNNDAINFQQLYYAERKHILNLDQQRCYK

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=375, Percent_Identity=36.8, Blast_Score=235, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 71441; Mature: 71310

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIPGKQFYYCFSCGAGGNAIKFLMEFQRQSFSDVVLELAKKYQVPIDTIEGPQQERLK
CCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
QQLSRRDTLYRVLKTATGWFRNQLNSPCGENALNYLKNKRNLSDGTLINFELGFAPDNWD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
SLLKYFVDIEKVSVEILESAGLIVPRKGGNGFYDRFRNRIIVPIHDRQKRVIGFGGRSLD
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCCEEEEEECCCCEEEECCCCCCC
GSEPKYLNSPETEIFEKGKNLFGFDKSTLSIRKKDYAVVVEGYFDVMALHDSGITNVVAS
CCCCCCCCCCHHHHHHCCCCCCCCCCHHHEEECCCEEEEEECCEEEEEECCCCHHHHHHH
LGTALSRNQITLLSRATDSKKILLNFDSDNAGIRAANRAISEVENLAIQGQLDLRVLQLP
HHHHHCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEECCCCEEEEECC
SGKDPDEFLKGNSPSEYEALAARSPLWMDWQIDQSLKDLDLSKSDQFQEAVSSLVSLLGK
CCCCHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LPQTAIRTHYLQKVAQRLSGGQGRFALQLEEDLRNQISGQRWHGRSKKIDKPQEISLRER
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCC
SESDILFTYIHCPNYRSFIRYELRLRDLDDFAINHHRAIWSTISNIEENMFGPETVEKIN
CCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RFNDSNNILADVDLIKKLLDNFLSSDNEHLPKLTPLLDVNELRLATLNDPESFIRGAMAA
HCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
LEKQKSLKRCRHLIDAWSSQRLQTLENCIASLIVQEKSEPSDSSDMEQRVISMFEDLNND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
AINFQQLYYAERKHILNLDQQRCYK
CCHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
SVIPGKQFYYCFSCGAGGNAIKFLMEFQRQSFSDVVLELAKKYQVPIDTIEGPQQERLK
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
QQLSRRDTLYRVLKTATGWFRNQLNSPCGENALNYLKNKRNLSDGTLINFELGFAPDNWD
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCHH
SLLKYFVDIEKVSVEILESAGLIVPRKGGNGFYDRFRNRIIVPIHDRQKRVIGFGGRSLD
HHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHCCCEEEEEECCCCEEEECCCCCCC
GSEPKYLNSPETEIFEKGKNLFGFDKSTLSIRKKDYAVVVEGYFDVMALHDSGITNVVAS
CCCCCCCCCCHHHHHHCCCCCCCCCCHHHEEECCCEEEEEECCEEEEEECCCCHHHHHHH
LGTALSRNQITLLSRATDSKKILLNFDSDNAGIRAANRAISEVENLAIQGQLDLRVLQLP
HHHHHCCCCEEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHEEECCCCEEEEECC
SGKDPDEFLKGNSPSEYEALAARSPLWMDWQIDQSLKDLDLSKSDQFQEAVSSLVSLLGK
CCCCHHHHHCCCCCCHHHHHHHCCCCEEEEECCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LPQTAIRTHYLQKVAQRLSGGQGRFALQLEEDLRNQISGQRWHGRSKKIDKPQEISLRER
CCHHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHCCCCCCCCCCCCCCCCCCCHHCC
SESDILFTYIHCPNYRSFIRYELRLRDLDDFAINHHRAIWSTISNIEENMFGPETVEKIN
CCCCEEEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
RFNDSNNILADVDLIKKLLDNFLSSDNEHLPKLTPLLDVNELRLATLNDPESFIRGAMAA
HCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHH
LEKQKSLKRCRHLIDAWSSQRLQTLENCIASLIVQEKSEPSDSSDMEQRVISMFEDLNND
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
AINFQQLYYAERKHILNLDQQRCYK
CCHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA