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The map label for this gene is gatA

Identifier: 124025640

GI number: 124025640

Start: 859859

End: 861319

Strand: Direct

Name: gatA

Synonym: NATL1_09331

Alternate gene names: 124025640

Gene position: 859859-861319 (Clockwise)

Preceding gene: 124025639

Following gene: 124025641

Centisome position: 46.11

GC content: 39.01

Gene sequence:

>1461_bases
ATGACTTTTGAAGACTTACGCCAGAAATTAAAAAGTGGTGAGGTTTCTTCCAAAGAACTTGTTCAAGAAAAAATAAACCG
AATAAATGAATTAGATCCAACTCTGAATTCCTTTTTAATTGTTAATACTGAGCTAGCTCTAAGCAAAGCAGAGCATATCG
ATAAACAAATCGCTTCTGGCGATCATTTACCGCCTTTGTCAGGAATACCCATTGCTATAAAAGACAATCTTTGTACAAAA
GGAATTAAGACAACTTGCGCAAGCAAGATTTTGGACAACTTTGTCCCCCCATACGAGTCAACAGTTACCAAAAAGCTTTT
GAACGCAGGAGCAATAATGATTGGCAAGACAAATATGGATGAATTTGCTATGGGGAGCTCTACAGAAACCTCTGCATTTG
GTCCTACTTTGAATCCATGGAACATTACAAAAGTTCCAGGAGGCAGCTCTGGTGGTAGCGCAGCTTCTGTTGCTGCCGGA
TTATGTTACGGATCTCTGGGTTCTGATACTGGCGGGTCAATCCGACAACCTGCTTCTTTTTGTGGCGTTGTTGGCATGAA
GCCAACTTATGGTCGAGTAAGTCGATGGGGGTTAATAGCTTTTGCTAGTTCTTTAGATCAGGTTGGTCCATTTGCGAATA
ATGTTTCTGATGCAGCTGAGATTTTGCAAGTTATATCTGGCAAGGATGAATTGGATTCAACTACTGTTGATATTCCCGTT
CCCAATTATTTAGAGACTCTTTCTAAGTCAATTAAGGGAATGAAAATAGGTTTAATTGATAACTGCTTTGATCATGAAGG
TTTAGCCACTGATGTTAAAGAATCTGTTCTTAGCTCTGCCTCGCTGCTAGAAAACTTAGGCGCAGAAATTGTTAATATTT
CTTGTCCTCGTTTCAATGATGGAATTGCTACTTATTACGTTATTGCCCCATCTGAAGCTTCAGCGAATTTAGCCAGATAT
GACGGAGTCAAATATGGATTCCGCGCTGAAGATGAACAATCTCTTATAGAGATGACCTCCAAAAGTCGAGCACTTGGTTT
TGGAAGTGAAGTTAAAAGAAGAATTCTTATTGGAACTTATGCTCTATCCGCTGGTTATGTTGATGCTTACTACAAAAAAG
CCCAGCAAGTTCGAACTTTGATACGTAGAGATTTTGATGATGCTTTTAAAAAAGTAGATGTTTTGTTAGCGCCAACAGCA
CCTACTACTGCTTTTGGTTCTGGAGATAATATTGATAATCCAATGGCGATGTATCTATCGGATCTATTAACTATTCCAGC
AAATTTGGCTGGATTGCCTGCAATCAGCCTGCCGTGTGGTTTTGATAAATCCGATTTGCCTATAGGCTTGCAGCTAATAG
GAAATGTTTTTGAAGAAGGAAAGCTTCTTCAAGTGGCTAATCAATTTGAGAAAGCTGCTGAGGTTTATAAAAATAGGCCT
AAAACAGATTTCACGTTATAA

Upstream 100 bases:

>100_bases
TGCAGAGTCACCCAAGAAAATTAATCATAGTTTAATTAGGTGTCGCCGTAATGAGCCTTTAACATCATAAGCTTAAAAAT
ATTTTTTTTATAATCTAATA

Downstream 100 bases:

>100_bases
AGTTTAAACCACTTCATGTGGAGAATATAATTATCGCTACACTATATGCAGTGTAGTTCCGTGGAATATTAATGGCTTTT
GTTCCTATTCATAACCATAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTFEDLRQKLKSGEVSSKELVQEKINRINELDPTLNSFLIVNTELALSKAEHIDKQIASGDHLPPLSGIPIAIKDNLCTK
GIKTTCASKILDNFVPPYESTVTKKLLNAGAIMIGKTNMDEFAMGSSTETSAFGPTLNPWNITKVPGGSSGGSAASVAAG
LCYGSLGSDTGGSIRQPASFCGVVGMKPTYGRVSRWGLIAFASSLDQVGPFANNVSDAAEILQVISGKDELDSTTVDIPV
PNYLETLSKSIKGMKIGLIDNCFDHEGLATDVKESVLSSASLLENLGAEIVNISCPRFNDGIATYYVIAPSEASANLARY
DGVKYGFRAEDEQSLIEMTSKSRALGFGSEVKRRILIGTYALSAGYVDAYYKKAQQVRTLIRRDFDDAFKKVDVLLAPTA
PTTAFGSGDNIDNPMAMYLSDLLTIPANLAGLPAISLPCGFDKSDLPIGLQLIGNVFEEGKLLQVANQFEKAAEVYKNRP
KTDFTL

Sequences:

>Translated_486_residues
MTFEDLRQKLKSGEVSSKELVQEKINRINELDPTLNSFLIVNTELALSKAEHIDKQIASGDHLPPLSGIPIAIKDNLCTK
GIKTTCASKILDNFVPPYESTVTKKLLNAGAIMIGKTNMDEFAMGSSTETSAFGPTLNPWNITKVPGGSSGGSAASVAAG
LCYGSLGSDTGGSIRQPASFCGVVGMKPTYGRVSRWGLIAFASSLDQVGPFANNVSDAAEILQVISGKDELDSTTVDIPV
PNYLETLSKSIKGMKIGLIDNCFDHEGLATDVKESVLSSASLLENLGAEIVNISCPRFNDGIATYYVIAPSEASANLARY
DGVKYGFRAEDEQSLIEMTSKSRALGFGSEVKRRILIGTYALSAGYVDAYYKKAQQVRTLIRRDFDDAFKKVDVLLAPTA
PTTAFGSGDNIDNPMAMYLSDLLTIPANLAGLPAISLPCGFDKSDLPIGLQLIGNVFEEGKLLQVANQFEKAAEVYKNRP
KTDFTL
>Mature_485_residues
TFEDLRQKLKSGEVSSKELVQEKINRINELDPTLNSFLIVNTELALSKAEHIDKQIASGDHLPPLSGIPIAIKDNLCTKG
IKTTCASKILDNFVPPYESTVTKKLLNAGAIMIGKTNMDEFAMGSSTETSAFGPTLNPWNITKVPGGSSGGSAASVAAGL
CYGSLGSDTGGSIRQPASFCGVVGMKPTYGRVSRWGLIAFASSLDQVGPFANNVSDAAEILQVISGKDELDSTTVDIPVP
NYLETLSKSIKGMKIGLIDNCFDHEGLATDVKESVLSSASLLENLGAEIVNISCPRFNDGIATYYVIAPSEASANLARYD
GVKYGFRAEDEQSLIEMTSKSRALGFGSEVKRRILIGTYALSAGYVDAYYKKAQQVRTLIRRDFDDAFKKVDVLLAPTAP
TTAFGSGDNIDNPMAMYLSDLLTIPANLAGLPAISLPCGFDKSDLPIGLQLIGNVFEEGKLLQVANQFEKAAEVYKNRPK
TDFTL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=498, Percent_Identity=39.7590361445783, Blast_Score=336, Evalue=3e-92,
Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=24.1379310344828, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI166795287, Length=291, Percent_Identity=28.8659793814433, Blast_Score=79, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=424, Percent_Identity=38.4433962264151, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71990152, Length=301, Percent_Identity=30.8970099667774, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17556264, Length=298, Percent_Identity=30.2013422818792, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17538252, Length=316, Percent_Identity=27.8481012658228, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=234, Percent_Identity=30.3418803418803, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17538254, Length=304, Percent_Identity=24.6710526315789, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=191, Percent_Identity=30.8900523560209, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556276, Length=191, Percent_Identity=30.8900523560209, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=422, Percent_Identity=36.7298578199052, Blast_Score=252, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6319685, Length=420, Percent_Identity=32.1428571428571, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6320448, Length=230, Percent_Identity=31.7391304347826, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24648113, Length=460, Percent_Identity=43.9130434782609, Blast_Score=338, Evalue=7e-93,
Organism=Drosophila melanogaster, GI24644968, Length=497, Percent_Identity=26.9617706237425, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24652985, Length=489, Percent_Identity=26.1758691206544, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI19922090, Length=489, Percent_Identity=26.1758691206544, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652981, Length=489, Percent_Identity=26.1758691206544, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652983, Length=489, Percent_Identity=26.1758691206544, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI161078093, Length=505, Percent_Identity=27.7227722772277, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI45550774, Length=508, Percent_Identity=23.6220472440945, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648435, Length=508, Percent_Identity=23.6220472440945, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648437, Length=508, Percent_Identity=23.6220472440945, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648441, Length=449, Percent_Identity=22.9398663697105, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648439, Length=449, Percent_Identity=22.9398663697105, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21356731, Length=232, Percent_Identity=28.0172413793103, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_PROM1 (A2C1Y1)

Other databases:

- EMBL:   CP000553
- RefSeq:   YP_001014756.1
- ProteinModelPortal:   A2C1Y1
- SMR:   A2C1Y1
- STRING:   A2C1Y1
- GeneID:   4780706
- GenomeReviews:   CP000553_GR
- KEGG:   pme:NATL1_09331
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   RWGLIAF
- ProtClustDB:   PRK00012
- BioCyc:   PMAR167555:NATL1_09331-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52045; Mature: 51914

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 74-74 ACT_SITE 149-149 ACT_SITE 173-173

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFEDLRQKLKSGEVSSKELVQEKINRINELDPTLNSFLIVNTELALSKAEHIDKQIASG
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
DHLPPLSGIPIAIKDNLCTKGIKTTCASKILDNFVPPYESTVTKKLLNAGAIMIGKTNMD
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCH
EFAMGSSTETSAFGPTLNPWNITKVPGGSSGGSAASVAAGLCYGSLGSDTGGSIRQPASF
HHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHH
CGVVGMKPTYGRVSRWGLIAFASSLDQVGPFANNVSDAAEILQVISGKDELDSTTVDIPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCC
PNYLETLSKSIKGMKIGLIDNCFDHEGLATDVKESVLSSASLLENLGAEIVNISCPRFND
CHHHHHHHHHHCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGFRAEDEQSLIEMTSKSRALGFGSEVKRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHEEEHH
ALSAGYVDAYYKKAQQVRTLIRRDFDDAFKKVDVLLAPTAPTTAFGSGDNIDNPMAMYLS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHH
DLLTIPANLAGLPAISLPCGFDKSDLPIGLQLIGNVFEEGKLLQVANQFEKAAEVYKNRP
HHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
KTDFTL
CCCCCC
>Mature Secondary Structure 
TFEDLRQKLKSGEVSSKELVQEKINRINELDPTLNSFLIVNTELALSKAEHIDKQIASG
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHCC
DHLPPLSGIPIAIKDNLCTKGIKTTCASKILDNFVPPYESTVTKKLLNAGAIMIGKTNMD
CCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCEEEEECCCCH
EFAMGSSTETSAFGPTLNPWNITKVPGGSSGGSAASVAAGLCYGSLGSDTGGSIRQPASF
HHHCCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHH
CGVVGMKPTYGRVSRWGLIAFASSLDQVGPFANNVSDAAEILQVISGKDELDSTTVDIPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCEEECCC
PNYLETLSKSIKGMKIGLIDNCFDHEGLATDVKESVLSSASLLENLGAEIVNISCPRFND
CHHHHHHHHHHCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGFRAEDEQSLIEMTSKSRALGFGSEVKRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHEEEHH
ALSAGYVDAYYKKAQQVRTLIRRDFDDAFKKVDVLLAPTAPTTAFGSGDNIDNPMAMYLS
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHH
DLLTIPANLAGLPAISLPCGFDKSDLPIGLQLIGNVFEEGKLLQVANQFEKAAEVYKNRP
HHHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCC
KTDFTL
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA