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The map label for this gene is ilvB [H]

Identifier: 124025294

GI number: 124025294

Start: 528967

End: 530730

Strand: Direct

Name: ilvB [H]

Synonym: NATL1_05831

Alternate gene names: 124025294

Gene position: 528967-530730 (Clockwise)

Preceding gene: 124025293

Following gene: 124025295

Centisome position: 28.37

GC content: 40.14

Gene sequence:

>1764_bases
GTGACCCTGACTTCTACGCCTAATCAAATAGAAGATTCAGGGACACAAACTCAACATGAGATGTCAGGAGCTGATGCCTT
GATGGATTCTCTTCGTAGGCATGGAGTTGATACTATTTTTGGTTATCCTGGCGGAGCAATCCTCCCAATATATGACGCGG
TTTTTAAAGCGGAGCAAGAGGGATGGTTAAAACACATTCTTGTTCGACATGAACAAGGTGGAACTCATGCTGCAGATGGT
TTCGCTAGAGCGACGGGAAAAGTAGGGGTTTGTTTTGGTACATCAGGTCCAGGCGCTACGAATCTAGTCACAGGAATAGC
AACTTCTCAGATGGATTCTGTACCTCTTGTCGTCATAACAGGACAAGTTCCTAGACCTGCTATTGGAACTGATGCTTTTC
AAGAAACAGATATTTTTGGAATAACACTTCCAATTGTTAAACATTCATGGGTCGTAAGAGATCCATCTGAAATTGCAAAA
GTTGTAGCGCAAGCCTTTCTCATCGCTTCGTCGGGTAGACCAGGCCCTGTTTTGATTGACATACCGAAAGATGTTGGACA
AGAGATGTTTAAATATCTTCCTGTTGAGCCTGGTTCAATAAAGCCACTAGGGTTTGAACTACCATTCGCGCCAGAACATA
AGGCCATAAGTTCAGCCTTAGATCTAATTGAAAATGCTGAGCAACCACTCCTTTACGTTGGCGGCGGTGTTATTTCTTCG
GGGGCACATGAAACTCTGGCTGCGATTGCTAATAGATATCAAATACCCGTAACAACAACTTTGATGGGAAAAGGCGCTTT
CGATGAACGTGATCCTCTATCAGTTGGAATGCTTGGTATGCATGGAACAGCTTATGCCAACTTTGCTGTGACTGAATGTG
ATTTGTTGATTGCCATTGGAGCAAGATTTGACGACAGAGTTACAGGTAAATTAGATACTTTTGCTCCTAAAGCAAAAGTA
ATTCATTTTGAGATTGATCCTGCTGAAATAAATAAAAATAGAGTTGTTGAAGTTTCTGTATTAGGTGATGTTGGAATTAG
TCTTGTTAAATTATTAGATCTCAGTAACCAAAGGAAAACAAATCCAAGAACTTCTCAATGGCTTAACAAAATTAAAAACT
GGAAAAATAATTTTCCTTTGATAACTCCCCCTAAAGAAGGAGAAATTTATCCACAGGAAGTTTTAATTGCGTTGAGAGAT
TTGGCTCAAGATGCTTACATTACAACTGATGTTGGACAACATCAGATGTGGGCAGCTCAGTATTTGTTGAATGGACCAAG
ACAATGGATTAGCAGTGCTGGACTTGGGACAATGGGCTTTGGAATGCCCGCTGCAATGGGAGTCAAAGTTGCTTTGCCTA
AGGAGAAAGTAATTTGTATCGCCGGTGATGCAAGTATCCTCATGAACATTCAGGAGCTTGGAACTATTGCTCAATATAAG
TTGAATTTAAAAGTAATTATCATCAATAATCATTGGCAAGGGATGGTTAGGCAATGGCAAGAAAGTTTTTATGATGAACG
ATATTCGGCATCTAACATGTCTGTTGGGGAGCCAGACTTTATTTCGCTATCTAAGGCTTTTGGAATTGAGGGTATAGTGA
TTTCAGAAAGAGAAAACCTAATTCCTCAACTCCAAAAAGCATTGGAGAATGAAGGACCAGTTCTTGTAAACGTAAATGTA
AGAAAAGGTGAAAATTGTTACCCTATGGTGCCACCTGGAAAAAGTAATGCTGAAATGGTTGGCATATCAGAGATTAAGAA
ATAA

Upstream 100 bases:

>100_bases
GCTGTTAAAACTTAGAGAATTTAATTAAATCTTTGCTTAATTTAGTTGAATTGAAATTATGCATACTAAGATTTATTGTT
AGTTTAGATTTTCTTCTGCC

Downstream 100 bases:

>100_bases
GTTAAAAACGTTTTATGAATAAATTTTTTACAAAGATTTTAATATTATTAGTTTCTCTTTTTTGGTTTCATTCGGCAGTT
GATGCCGCTGAAGTTTTACA

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTLTSTPNQIEDSGTQTQHEMSGADALMDSLRRHGVDTIFGYPGGAILPIYDAVFKAEQEGWLKHILVRHEQGGTHAADG
FARATGKVGVCFGTSGPGATNLVTGIATSQMDSVPLVVITGQVPRPAIGTDAFQETDIFGITLPIVKHSWVVRDPSEIAK
VVAQAFLIASSGRPGPVLIDIPKDVGQEMFKYLPVEPGSIKPLGFELPFAPEHKAISSALDLIENAEQPLLYVGGGVISS
GAHETLAAIANRYQIPVTTTLMGKGAFDERDPLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPKAKV
IHFEIDPAEINKNRVVEVSVLGDVGISLVKLLDLSNQRKTNPRTSQWLNKIKNWKNNFPLITPPKEGEIYPQEVLIALRD
LAQDAYITTDVGQHQMWAAQYLLNGPRQWISSAGLGTMGFGMPAAMGVKVALPKEKVICIAGDASILMNIQELGTIAQYK
LNLKVIIINNHWQGMVRQWQESFYDERYSASNMSVGEPDFISLSKAFGIEGIVISERENLIPQLQKALENEGPVLVNVNV
RKGENCYPMVPPGKSNAEMVGISEIKK

Sequences:

>Translated_587_residues
MTLTSTPNQIEDSGTQTQHEMSGADALMDSLRRHGVDTIFGYPGGAILPIYDAVFKAEQEGWLKHILVRHEQGGTHAADG
FARATGKVGVCFGTSGPGATNLVTGIATSQMDSVPLVVITGQVPRPAIGTDAFQETDIFGITLPIVKHSWVVRDPSEIAK
VVAQAFLIASSGRPGPVLIDIPKDVGQEMFKYLPVEPGSIKPLGFELPFAPEHKAISSALDLIENAEQPLLYVGGGVISS
GAHETLAAIANRYQIPVTTTLMGKGAFDERDPLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPKAKV
IHFEIDPAEINKNRVVEVSVLGDVGISLVKLLDLSNQRKTNPRTSQWLNKIKNWKNNFPLITPPKEGEIYPQEVLIALRD
LAQDAYITTDVGQHQMWAAQYLLNGPRQWISSAGLGTMGFGMPAAMGVKVALPKEKVICIAGDASILMNIQELGTIAQYK
LNLKVIIINNHWQGMVRQWQESFYDERYSASNMSVGEPDFISLSKAFGIEGIVISERENLIPQLQKALENEGPVLVNVNV
RKGENCYPMVPPGKSNAEMVGISEIKK
>Mature_586_residues
TLTSTPNQIEDSGTQTQHEMSGADALMDSLRRHGVDTIFGYPGGAILPIYDAVFKAEQEGWLKHILVRHEQGGTHAADGF
ARATGKVGVCFGTSGPGATNLVTGIATSQMDSVPLVVITGQVPRPAIGTDAFQETDIFGITLPIVKHSWVVRDPSEIAKV
VAQAFLIASSGRPGPVLIDIPKDVGQEMFKYLPVEPGSIKPLGFELPFAPEHKAISSALDLIENAEQPLLYVGGGVISSG
AHETLAAIANRYQIPVTTTLMGKGAFDERDPLSVGMLGMHGTAYANFAVTECDLLIAIGARFDDRVTGKLDTFAPKAKVI
HFEIDPAEINKNRVVEVSVLGDVGISLVKLLDLSNQRKTNPRTSQWLNKIKNWKNNFPLITPPKEGEIYPQEVLIALRDL
AQDAYITTDVGQHQMWAAQYLLNGPRQWISSAGLGTMGFGMPAAMGVKVALPKEKVICIAGDASILMNIQELGTIAQYKL
NLKVIIINNHWQGMVRQWQESFYDERYSASNMSVGEPDFISLSKAFGIEGIVISERENLIPQLQKALENEGPVLVNVNVR
KGENCYPMVPPGKSNAEMVGISEIKK

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=570, Percent_Identity=27.1929824561404, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI21361361, Length=587, Percent_Identity=24.190800681431, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI87081685, Length=568, Percent_Identity=46.1267605633803, Blast_Score=490, Evalue=1e-139,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=44.1124780316344, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1786717, Length=565, Percent_Identity=31.858407079646, Blast_Score=302, Evalue=5e-83,
Organism=Escherichia coli, GI1787096, Length=549, Percent_Identity=27.6867030965392, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1788716, Length=547, Percent_Identity=26.508226691042, Blast_Score=162, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17542570, Length=601, Percent_Identity=26.955074875208, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17531301, Length=562, Percent_Identity=26.8683274021352, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17531299, Length=562, Percent_Identity=26.8683274021352, Blast_Score=143, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323755, Length=589, Percent_Identity=43.2937181663837, Blast_Score=446, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=496, Percent_Identity=25, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6321524, Length=497, Percent_Identity=24.3460764587525, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323073, Length=499, Percent_Identity=23.0460921843687, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323163, Length=518, Percent_Identity=23.1660231660232, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320588, Length=519, Percent_Identity=21.7726396917148, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19922626, Length=574, Percent_Identity=25.609756097561, Blast_Score=169, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 63632; Mature: 63501

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTSTPNQIEDSGTQTQHEMSGADALMDSLRRHGVDTIFGYPGGAILPIYDAVFKAEQE
CCCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
GWLKHILVRHEQGGTHAADGFARATGKVGVCFGTSGPGATNLVTGIATSQMDSVPLVVIT
HHHHHHHEEECCCCCCHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVVRDPSEIAKVVAQAFLIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEHHHCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEE
IPKDVGQEMFKYLPVEPGSIKPLGFELPFAPEHKAISSALDLIENAEQPLLYVGGGVISS
CCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCEECC
GAHETLAAIANRYQIPVTTTLMGKGAFDERDPLSVGMLGMHGTAYANFAVTECDLLIAIG
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECEEEEEEC
ARFDDRVTGKLDTFAPKAKVIHFEIDPAEINKNRVVEVSVLGDVGISLVKLLDLSNQRKT
CCCCCCCCCCHHCCCCCEEEEEEECCHHHCCCCEEEEEEEECHHHHHHHHHHHCCCCCCC
NPRTSQWLNKIKNWKNNFPLITPPKEGEIYPQEVLIALRDLAQDAYITTDVGQHQMWAAQ
CCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
YLLNGPRQWISSAGLGTMGFGMPAAMGVKVALPKEKVICIAGDASILMNIQELGTIAQYK
HHHCCHHHHHHHCCCCCCCCCCHHHCCEEEECCCCCEEEEECCHHHEEEHHHHCCEEEEE
LNLKVIIINNHWQGMVRQWQESFYDERYSASNMSVGEPDFISLSKAFGIEGIVISERENL
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHCCCCEEEECCHHHH
IPQLQKALENEGPVLVNVNVRKGENCYPMVPPGKSNAEMVGISEIKK
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEECHHHHCC
>Mature Secondary Structure 
TLTSTPNQIEDSGTQTQHEMSGADALMDSLRRHGVDTIFGYPGGAILPIYDAVFKAEQE
CCCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHH
GWLKHILVRHEQGGTHAADGFARATGKVGVCFGTSGPGATNLVTGIATSQMDSVPLVVIT
HHHHHHHEEECCCCCCHHCCHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCEEEEE
GQVPRPAIGTDAFQETDIFGITLPIVKHSWVVRDPSEIAKVVAQAFLIASSGRPGPVLID
CCCCCCCCCCCCCCCCCEEEEEEHHHCCCEEEECHHHHHHHHHHHHHHCCCCCCCCEEEE
IPKDVGQEMFKYLPVEPGSIKPLGFELPFAPEHKAISSALDLIENAEQPLLYVGGGVISS
CCHHHHHHHHHHCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCEECC
GAHETLAAIANRYQIPVTTTLMGKGAFDERDPLSVGMLGMHGTAYANFAVTECDLLIAIG
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECEEEEEEC
ARFDDRVTGKLDTFAPKAKVIHFEIDPAEINKNRVVEVSVLGDVGISLVKLLDLSNQRKT
CCCCCCCCCCHHCCCCCEEEEEEECCHHHCCCCEEEEEEEECHHHHHHHHHHHCCCCCCC
NPRTSQWLNKIKNWKNNFPLITPPKEGEIYPQEVLIALRDLAQDAYITTDVGQHQMWAAQ
CCHHHHHHHHHHHHCCCCCEECCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHH
YLLNGPRQWISSAGLGTMGFGMPAAMGVKVALPKEKVICIAGDASILMNIQELGTIAQYK
HHHCCHHHHHHHCCCCCCCCCCHHHCCEEEECCCCCEEEEECCHHHEEEHHHHCCEEEEE
LNLKVIIINNHWQGMVRQWQESFYDERYSASNMSVGEPDFISLSKAFGIEGIVISERENL
EEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHCCCCEEEECCHHHH
IPQLQKALENEGPVLVNVNVRKGENCYPMVPPGKSNAEMVGISEIKK
HHHHHHHHCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]