Click here to switch to the map view.

The map label for this gene is priA [H]

Identifier: 124025266

GI number: 124025266

Start: 502621

End: 504864

Strand: Direct

Name: priA [H]

Synonym: NATL1_05551

Alternate gene names: 124025266

Gene position: 502621-504864 (Clockwise)

Preceding gene: 124025265

Following gene: 124025270

Centisome position: 26.95

GC content: 36.76

Gene sequence:

>2244_bases
ATGAATTCTTTTATATTTGATATTTGGTTACATGTAGGTCGTGAAGGGCGATGTTTTTCTTATCAAGATGGAAATAATTT
AGACATTGATTTAGGCGATGTTGTGACAGTGCGTCTGAAAGGGCAACGCATGCAAGGGTTGGTTGTTAAGAAGATGAAAA
AAAACATAAATAGTACACACCAAAATTTAAATAATTTTTCGCTGAATAATGTAGAAACATTGGTTCAAAAAGCAGCTATC
AAAAAAGAATGGAGAGAGTGGCTAGAAGAAATTGCTCTTGATTTATATGTAAGTGATTTTCAGATGCTTAAGACCGCGTT
ACCTCCTGGTTGGTTAGGAAGATCGAAACTATCGAATAGACCTAAAAAACTCTGGTGGGTAAAATTGTCTAGCAATAATT
ATGAGGGAAAGATATCTTCTCGACAGATTGAGTTAAAGAAAAATCTTCTCTTAAATGGAGGAGGAAAATGGCAAAAGGAT
TTGGAGGCTGAAGGATTTTCTTCTGTATTGATTAGAAACTTTGTCTCAGTTGGTTGCGGAGAAAGAGAAAAACGTTTTTA
TCTTTTTAATTCTTTTGATAATGAAGAATCTAATGAAAAAAAGATGTTAAAGATTGAGGAACCTCAACCTTTAACACTTG
AGCAAAAATTAGCAAAAGAAAAATATGAGTCTCTTCCAAACGGATCAGCTCTTTTGCTTTGGGGTGTTACTGGGTCTGGG
AAGACGGAGGTATACCTACAAATTGCAGCGCAAGAGTTATCTGAAAGTAGACATTGTCTTATCCTTACACCAGAAATTGG
CTTAGTACCACAATTAGTTGATCGCTTTCGAAAAAGATTTGGGTTAAATGTTTTTGAATATCATAGTAATTGTTCTCCTA
AAGAGAAAATTGAGACATGGAAGAGAGCTTTAGACAACACAAAACCTAGTGTTTTTATTGGTACTCGATCAGCTATTTTT
CTGCCTTTATCCAGCTTAGGATTGATAGTTCTTGATGAAGAACACGATAGCTCTTTTAAACAGGAATCCCCTATGCCTTG
CTATCATGCAAGAGAATTGGCAATTCATAGAGCAAAAAAAACAAGTGCTAAAGTAATACTTGGAACAGCAACTCCATCTT
TGAATGTTTGGAAAAATTTAAAACCAAATGGAAATGTAGTTGTTGCAAAATTAACCAAAAGGATTTCGAATCGTAAATTA
CCAACGGTTAGTGTAGTAGACATGCGAGAGGAATTAGCGCTTGGAAATCGAAGCTTAATTAGTAGATATCTAAAAAAACA
ACTTTTGAGTATAAAAGAGAGTGGAAATCAAGCTATTGTTTTAGTCCCTAGACGTGGATATAGTAGTTTTTTAAGTTGCC
GCAGTTGTGGAGAGGTCGTTCAATGTCCACATTGTGATATTTCACTAACTGTACATCGTTCTAAAGAGGGTAATCAATGG
TTGCGTTGTCATTGGTGCGACTTTCGTTCGAAAATTAGTGATAAGTGTGGAGAATGTGGTTCAAATGCTTTTAAACCGTT
TGGGACTGGAACACAAAGAGTGATGGATCATTTAGAAAGAGAACTAGAGGGTATAAGTTTATTAAGGTTTGATAGAGATA
CAACTAGAGGCCGTGATGGCCATAGATTGTTGCTAGAAAGATTTGCTGATGGTGACGCCGATATTTTAGTAGGTACTCAG
ATGCTATCTAAGGGAATGGATTTACCAAAGGTAACTCTTGCCGTCGTCTTAGCAGCAGATGGTTTATTGTATCGTCCTGA
TTTAATGGCTACTGAAGAAACGCTTCAATTATTTATGCAATTAGCTGGTCGTGCAGGGCGAGGTGAGCAACCTGGAAAGG
TTGTAGTGCAAACTTATTGTCCTGATCATCCAGTGATTCTTCATTTGATTGATGGGAGTTACGAAGAATTTCTGAAAAAA
GAAGAGAAGACTAGAAAAGAAGCCTCGATGGTTCCATACAGTCGAGCCTGCTTATTAAGGTTCTCTGGCGAATCTTCAGA
GTTGGCATCACAAGGAGCATTTAATGTTTTATCAAAAATAAAGAATGCTTGCAGTCAAAAAGGTTGGAAATTAGTCGGTC
CAGCACCTTCATTAGTTGAGAGAGTCGCAGGTAAAAGCCGTTGGCAACTTCTTTTGTACGGTCCAGAATCAAGTCATATA
CCACTCCCTTATGGACCTGAATTATGGAAAGATTTACCAAAAGGAGTAACTCTTTCTATTGATCCTGATCCTCTACAATT
ATGA

Upstream 100 bases:

>100_bases
TCCCTCTGGGTGGAATTTTGCAAGTTCCACAACAGCTTTAAAGCTGTTCACAATCCAATGGTAGAAAAAATTTCTAAAGT
CGGCAAGTATTAAAACAGAT

Downstream 100 bases:

>100_bases
GTTTTATCTTTTTTTAAGGTGGTAGCTCGGGGAAACCGAAGCCCATTTGTAGACGTAATCTCTGAAGGGATAAGCTCAGC
AAAGTTAAAGCAATTATCAA

Product: primosomal protein N' (replication factor Y)

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MNSFIFDIWLHVGREGRCFSYQDGNNLDIDLGDVVTVRLKGQRMQGLVVKKMKKNINSTHQNLNNFSLNNVETLVQKAAI
KKEWREWLEEIALDLYVSDFQMLKTALPPGWLGRSKLSNRPKKLWWVKLSSNNYEGKISSRQIELKKNLLLNGGGKWQKD
LEAEGFSSVLIRNFVSVGCGEREKRFYLFNSFDNEESNEKKMLKIEEPQPLTLEQKLAKEKYESLPNGSALLLWGVTGSG
KTEVYLQIAAQELSESRHCLILTPEIGLVPQLVDRFRKRFGLNVFEYHSNCSPKEKIETWKRALDNTKPSVFIGTRSAIF
LPLSSLGLIVLDEEHDSSFKQESPMPCYHARELAIHRAKKTSAKVILGTATPSLNVWKNLKPNGNVVVAKLTKRISNRKL
PTVSVVDMREELALGNRSLISRYLKKQLLSIKESGNQAIVLVPRRGYSSFLSCRSCGEVVQCPHCDISLTVHRSKEGNQW
LRCHWCDFRSKISDKCGECGSNAFKPFGTGTQRVMDHLERELEGISLLRFDRDTTRGRDGHRLLLERFADGDADILVGTQ
MLSKGMDLPKVTLAVVLAADGLLYRPDLMATEETLQLFMQLAGRAGRGEQPGKVVVQTYCPDHPVILHLIDGSYEEFLKK
EEKTRKEASMVPYSRACLLRFSGESSELASQGAFNVLSKIKNACSQKGWKLVGPAPSLVERVAGKSRWQLLLYGPESSHI
PLPYGPELWKDLPKGVTLSIDPDPLQL

Sequences:

>Translated_747_residues
MNSFIFDIWLHVGREGRCFSYQDGNNLDIDLGDVVTVRLKGQRMQGLVVKKMKKNINSTHQNLNNFSLNNVETLVQKAAI
KKEWREWLEEIALDLYVSDFQMLKTALPPGWLGRSKLSNRPKKLWWVKLSSNNYEGKISSRQIELKKNLLLNGGGKWQKD
LEAEGFSSVLIRNFVSVGCGEREKRFYLFNSFDNEESNEKKMLKIEEPQPLTLEQKLAKEKYESLPNGSALLLWGVTGSG
KTEVYLQIAAQELSESRHCLILTPEIGLVPQLVDRFRKRFGLNVFEYHSNCSPKEKIETWKRALDNTKPSVFIGTRSAIF
LPLSSLGLIVLDEEHDSSFKQESPMPCYHARELAIHRAKKTSAKVILGTATPSLNVWKNLKPNGNVVVAKLTKRISNRKL
PTVSVVDMREELALGNRSLISRYLKKQLLSIKESGNQAIVLVPRRGYSSFLSCRSCGEVVQCPHCDISLTVHRSKEGNQW
LRCHWCDFRSKISDKCGECGSNAFKPFGTGTQRVMDHLERELEGISLLRFDRDTTRGRDGHRLLLERFADGDADILVGTQ
MLSKGMDLPKVTLAVVLAADGLLYRPDLMATEETLQLFMQLAGRAGRGEQPGKVVVQTYCPDHPVILHLIDGSYEEFLKK
EEKTRKEASMVPYSRACLLRFSGESSELASQGAFNVLSKIKNACSQKGWKLVGPAPSLVERVAGKSRWQLLLYGPESSHI
PLPYGPELWKDLPKGVTLSIDPDPLQL
>Mature_747_residues
MNSFIFDIWLHVGREGRCFSYQDGNNLDIDLGDVVTVRLKGQRMQGLVVKKMKKNINSTHQNLNNFSLNNVETLVQKAAI
KKEWREWLEEIALDLYVSDFQMLKTALPPGWLGRSKLSNRPKKLWWVKLSSNNYEGKISSRQIELKKNLLLNGGGKWQKD
LEAEGFSSVLIRNFVSVGCGEREKRFYLFNSFDNEESNEKKMLKIEEPQPLTLEQKLAKEKYESLPNGSALLLWGVTGSG
KTEVYLQIAAQELSESRHCLILTPEIGLVPQLVDRFRKRFGLNVFEYHSNCSPKEKIETWKRALDNTKPSVFIGTRSAIF
LPLSSLGLIVLDEEHDSSFKQESPMPCYHARELAIHRAKKTSAKVILGTATPSLNVWKNLKPNGNVVVAKLTKRISNRKL
PTVSVVDMREELALGNRSLISRYLKKQLLSIKESGNQAIVLVPRRGYSSFLSCRSCGEVVQCPHCDISLTVHRSKEGNQW
LRCHWCDFRSKISDKCGECGSNAFKPFGTGTQRVMDHLERELEGISLLRFDRDTTRGRDGHRLLLERFADGDADILVGTQ
MLSKGMDLPKVTLAVVLAADGLLYRPDLMATEETLQLFMQLAGRAGRGEQPGKVVVQTYCPDHPVILHLIDGSYEEFLKK
EEKTRKEASMVPYSRACLLRFSGESSELASQGAFNVLSKIKNACSQKGWKLVGPAPSLVERVAGKSRWQLLLYGPESSHI
PLPYGPELWKDLPKGVTLSIDPDPLQL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=494, Percent_Identity=37.0445344129555, Blast_Score=329, Evalue=5e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84331; Mature: 84331

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFIFDIWLHVGREGRCFSYQDGNNLDIDLGDVVTVRLKGQRMQGLVVKKMKKNINSTH
CCCEEEEEHHHCCCCCCEEEECCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
QNLNNFSLNNVETLVQKAAIKKEWREWLEEIALDLYVSDFQMLKTALPPGWLGRSKLSNR
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
PKKLWWVKLSSNNYEGKISSRQIELKKNLLLNGGGKWQKDLEAEGFSSVLIRNFVSVGCG
CCEEEEEEECCCCCCCCCCCCEEEEECCEEECCCCCCCHHHCCCCHHHHHHHHHHHCCCC
EREKRFYLFNSFDNEESNEKKMLKIEEPQPLTLEQKLAKEKYESLPNGSALLLWGVTGSG
CCCCEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
KTEVYLQIAAQELSESRHCLILTPEIGLVPQLVDRFRKRFGLNVFEYHSNCSPKEKIETW
CCEEEEEEEHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHH
KRALDNTKPSVFIGTRSAIFLPLSSLGLIVLDEEHDSSFKQESPMPCYHARELAIHRAKK
HHHHCCCCCEEEEECCCEEEEEHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC
TSAKVILGTATPSLNVWKNLKPNGNVVVAKLTKRISNRKLPTVSVVDMREELALGNRSLI
CCCEEEEEECCCCCHHHCCCCCCCCEEEEEHHHHHHCCCCCEEEHHHHHHHHHCCCHHHH
SRYLKKQLLSIKESGNQAIVLVPRRGYSSFLSCRSCGEVVQCPHCDISLTVHRSKEGNQW
HHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCCE
LRCHWCDFRSKISDKCGECGSNAFKPFGTGTQRVMDHLERELEGISLLRFDRDTTRGRDG
EEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
HRLLLERFADGDADILVGTQMLSKGMDLPKVTLAVVLAADGLLYRPDLMATEETLQLFMQ
HHHHHHHHCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHH
LAGRAGRGEQPGKVVVQTYCPDHPVILHLIDGSYEEFLKKEEKTRKEASMVPYSRACLLR
HHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
FSGESSELASQGAFNVLSKIKNACSQKGWKLVGPAPSLVERVAGKSRWQLLLYGPESSHI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCEEEEEEECCCCCCC
PLPYGPELWKDLPKGVTLSIDPDPLQL
CCCCCHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MNSFIFDIWLHVGREGRCFSYQDGNNLDIDLGDVVTVRLKGQRMQGLVVKKMKKNINSTH
CCCEEEEEHHHCCCCCCEEEECCCCCCEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHH
QNLNNFSLNNVETLVQKAAIKKEWREWLEEIALDLYVSDFQMLKTALPPGWLGRSKLSNR
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCC
PKKLWWVKLSSNNYEGKISSRQIELKKNLLLNGGGKWQKDLEAEGFSSVLIRNFVSVGCG
CCEEEEEEECCCCCCCCCCCCEEEEECCEEECCCCCCCHHHCCCCHHHHHHHHHHHCCCC
EREKRFYLFNSFDNEESNEKKMLKIEEPQPLTLEQKLAKEKYESLPNGSALLLWGVTGSG
CCCCEEEEEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCC
KTEVYLQIAAQELSESRHCLILTPEIGLVPQLVDRFRKRFGLNVFEYHSNCSPKEKIETW
CCEEEEEEEHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHH
KRALDNTKPSVFIGTRSAIFLPLSSLGLIVLDEEHDSSFKQESPMPCYHARELAIHRAKK
HHHHCCCCCEEEEECCCEEEEEHHHCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCC
TSAKVILGTATPSLNVWKNLKPNGNVVVAKLTKRISNRKLPTVSVVDMREELALGNRSLI
CCCEEEEEECCCCCHHHCCCCCCCCEEEEEHHHHHHCCCCCEEEHHHHHHHHHCCCHHHH
SRYLKKQLLSIKESGNQAIVLVPRRGYSSFLSCRSCGEVVQCPHCDISLTVHRSKEGNQW
HHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHHHCCHHHCCCCCCEEEEEEECCCCCCE
LRCHWCDFRSKISDKCGECGSNAFKPFGTGTQRVMDHLERELEGISLLRFDRDTTRGRDG
EEEEECCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCH
HRLLLERFADGDADILVGTQMLSKGMDLPKVTLAVVLAADGLLYRPDLMATEETLQLFMQ
HHHHHHHHCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHCCCCEECCCCCCHHHHHHHHHH
LAGRAGRGEQPGKVVVQTYCPDHPVILHLIDGSYEEFLKKEEKTRKEASMVPYSRACLLR
HHCCCCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
FSGESSELASQGAFNVLSKIKNACSQKGWKLVGPAPSLVERVAGKSRWQLLLYGPESSHI
ECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHHHHCCCCEEEEEEECCCCCCC
PLPYGPELWKDLPKGVTLSIDPDPLQL
CCCCCHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]