Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 124025240

GI number: 124025240

Start: 480002

End: 481480

Strand: Reverse

Name: gltX [H]

Synonym: NATL1_05291

Alternate gene names: 124025240

Gene position: 481480-480002 (Counterclockwise)

Preceding gene: 124025241

Following gene: 124025235

Centisome position: 25.82

GC content: 35.5

Gene sequence:

>1479_bases
TTGCGGGTTCGAATCCCGTCGGTCCCGTATCTAAATCGAATTAGAGCTTTGAAAGTTAGGGTAAGATTGGCACCAAGTCC
AACTGGAACACTTCATCTAGGAACAGCGAGAACGGCACTATTTAACTGGCTATTTGCAAAAAAAGAAGGTGGAACTTTTC
TCTTAAGAATTGAAGATACAGATATTGAAAGATCAAGAGAAGAGTATATAAACGATATATATGATGGTCTCAAGTGGCTA
GGAATCAACTGGGATGAATCCCCAACAATTCAAAGTGAAAGAGTAAATGAACATAAACAAATTATAAAAACTCTCGTCGA
TAAAGGATTTGCATATAAATGTTATGCATCAGAAGCTGAACTAGATGAAATGAGAGAAACTCAAAAGAGAAATGGTTTAG
CGCCAAAATATGACAATAGGCACAGAAACTTAACTCCTGAGCAAGAATCAGAATTTATAAAGCTTGGGAGAGATCCTGTT
ATCAGATTCAAAATCAGTGATGAAAAATTAATTTCATGGAATGACTTGATTCGTGGAAAAATGACTTGGAGTGGAAAAGA
TTTAGGAGGTGATATGGTCATCGCAAGAAGAGCACCAGGGAATTCAATTGGTGATCCCTTATACAATTTAGTTGTGGTAG
CTGATGATTCAGCAATGAAAATTTCTCATGTAATAAGGGGAGAAGATCATCTTGCAAATACAGCGAAACAAATTCTTCTC
TACGAAGCTCTTGATCTAAATATTCCCGTTTTTGCTCATACTCCTTTAATTCTTAATTCTGAAGGTAAAAAACTTTCCAA
GAGGGATGGAGTTACTTCCATTTCTGAATTCAAGAAAATGGGTTACACATCTGAAGCGATGGCTAATTATATGACTCTTC
TTGGCTGGTCAGTTCCAGAAGGAATTAATGAAAGATTCAACATTTCAGAAGTTTCAGAGATTTTCAGTTTTAAAAAGGTT
AATAAAGCTTCAGCAAAATTTGATTGGGACAAATTGAACTGGCTCAACTCTCAAGTAATCCATGAGATGTCAGCAGAAAC
TCTATTAGAAAATTTAGAACCTTTATTTAAAGAAAATGGTTGGCATCTTCCAAGTCATGAATGGGGCATCAATCTAGTAG
GTTTAATAGGGCCATCCATGGTTCTTATTAATGATGGAGTAGACCAAGCTAAACCTTTTTTTGAAGAGCAAGAATTAAGT
GATGATGGCAAAAAGCAATTGGAAATCAAAGAAGCAGCTGTAATTTTAAAGTTTATTCTTGAAAAGTTGGCGGACTCAGA
TGCTGCATCTTTTTCTAAAGAAAAAGCTCTTGATTTAATTGATCAAGCTACAAAAAGCTGCAAAGTAAAAAAAGGTGTTG
TTATGAAAAGTCTTAGAGCTGCACTTTTTGGAACTCTCAATGGTCCTGATTTAATTCAAAGTTGGGTTTTACTCTCAAGA
TTCAGTAAAGATAGAGCCCGAATTAGTCGGCTTATCTAA

Upstream 100 bases:

>100_bases
CAATACAAAGGATTTCTTAATTAAAGATTCAATCCTTTTCTGGGCCTTAAACTGGGACCGTAGTTCAACTGGTTAGAGCA
CCGCCCTGTCACGGCGGAAG

Downstream 100 bases:

>100_bases
ATCTTTATCTTCATCCAGTAGACCCAACATTTTTGCCAATGGTTGAGCTGTTAAACCTTGAATACCTACTGTCATTAATA
TTGTCAAAAAGACTAATCCC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MRVRIPSVPYLNRIRALKVRVRLAPSPTGTLHLGTARTALFNWLFAKKEGGTFLLRIEDTDIERSREEYINDIYDGLKWL
GINWDESPTIQSERVNEHKQIIKTLVDKGFAYKCYASEAELDEMRETQKRNGLAPKYDNRHRNLTPEQESEFIKLGRDPV
IRFKISDEKLISWNDLIRGKMTWSGKDLGGDMVIARRAPGNSIGDPLYNLVVVADDSAMKISHVIRGEDHLANTAKQILL
YEALDLNIPVFAHTPLILNSEGKKLSKRDGVTSISEFKKMGYTSEAMANYMTLLGWSVPEGINERFNISEVSEIFSFKKV
NKASAKFDWDKLNWLNSQVIHEMSAETLLENLEPLFKENGWHLPSHEWGINLVGLIGPSMVLINDGVDQAKPFFEEQELS
DDGKKQLEIKEAAVILKFILEKLADSDAASFSKEKALDLIDQATKSCKVKKGVVMKSLRAALFGTLNGPDLIQSWVLLSR
FSKDRARISRLI

Sequences:

>Translated_492_residues
MRVRIPSVPYLNRIRALKVRVRLAPSPTGTLHLGTARTALFNWLFAKKEGGTFLLRIEDTDIERSREEYINDIYDGLKWL
GINWDESPTIQSERVNEHKQIIKTLVDKGFAYKCYASEAELDEMRETQKRNGLAPKYDNRHRNLTPEQESEFIKLGRDPV
IRFKISDEKLISWNDLIRGKMTWSGKDLGGDMVIARRAPGNSIGDPLYNLVVVADDSAMKISHVIRGEDHLANTAKQILL
YEALDLNIPVFAHTPLILNSEGKKLSKRDGVTSISEFKKMGYTSEAMANYMTLLGWSVPEGINERFNISEVSEIFSFKKV
NKASAKFDWDKLNWLNSQVIHEMSAETLLENLEPLFKENGWHLPSHEWGINLVGLIGPSMVLINDGVDQAKPFFEEQELS
DDGKKQLEIKEAAVILKFILEKLADSDAASFSKEKALDLIDQATKSCKVKKGVVMKSLRAALFGTLNGPDLIQSWVLLSR
FSKDRARISRLI
>Mature_492_residues
MRVRIPSVPYLNRIRALKVRVRLAPSPTGTLHLGTARTALFNWLFAKKEGGTFLLRIEDTDIERSREEYINDIYDGLKWL
GINWDESPTIQSERVNEHKQIIKTLVDKGFAYKCYASEAELDEMRETQKRNGLAPKYDNRHRNLTPEQESEFIKLGRDPV
IRFKISDEKLISWNDLIRGKMTWSGKDLGGDMVIARRAPGNSIGDPLYNLVVVADDSAMKISHVIRGEDHLANTAKQILL
YEALDLNIPVFAHTPLILNSEGKKLSKRDGVTSISEFKKMGYTSEAMANYMTLLGWSVPEGINERFNISEVSEIFSFKKV
NKASAKFDWDKLNWLNSQVIHEMSAETLLENLEPLFKENGWHLPSHEWGINLVGLIGPSMVLINDGVDQAKPFFEEQELS
DDGKKQLEIKEAAVILKFILEKLADSDAASFSKEKALDLIDQATKSCKVKKGVVMKSLRAALFGTLNGPDLIQSWVLLSR
FSKDRARISRLI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=331, Percent_Identity=39.8791540785499, Blast_Score=222, Evalue=8e-58,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=36.7170626349892, Blast_Score=294, Evalue=7e-81,
Organism=Escherichia coli, GI226510925, Length=256, Percent_Identity=30.46875, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786895, Length=122, Percent_Identity=28.6885245901639, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=324, Percent_Identity=37.962962962963, Blast_Score=192, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6324540, Length=470, Percent_Identity=33.8297872340426, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6324742, Length=144, Percent_Identity=31.9444444444444, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=32.0987654320988, Blast_Score=224, Evalue=1e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 55808; Mature: 55808

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRIPSVPYLNRIRALKVRVRLAPSPTGTLHLGTARTALFNWLFAKKEGGTFLLRIEDT
CEECCCCCCHHHHHEEEEEEEEECCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECC
DIERSREEYINDIYDGLKWLGINWDESPTIQSERVNEHKQIIKTLVDKGFAYKCYASEAE
CHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCH
LDEMRETQKRNGLAPKYDNRHRNLTPEQESEFIKLGRDPVIRFKISDEKLISWNDLIRGK
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEECHHHHHCCC
MTWSGKDLGGDMVIARRAPGNSIGDPLYNLVVVADDSAMKISHVIRGEDHLANTAKQILL
EEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHH
YEALDLNIPVFAHTPLILNSEGKKLSKRDGVTSISEFKKMGYTSEAMANYMTLLGWSVPE
HHHHCCCCCEEECCCEEECCCCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCC
GINERFNISEVSEIFSFKKVNKASAKFDWDKLNWLNSQVIHEMSAETLLENLEPLFKENG
CCCCCCCHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
WHLPSHEWGINLVGLIGPSMVLINDGVDQAKPFFEEQELSDDGKKQLEIKEAAVILKFIL
CCCCCCCCCCEEEEECCCCEEEEECCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
EKLADSDAASFSKEKALDLIDQATKSCKVKKGVVMKSLRAALFGTLNGPDLIQSWVLLSR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FSKDRARISRLI
HHHHHHHHHHCC
>Mature Secondary Structure
MRVRIPSVPYLNRIRALKVRVRLAPSPTGTLHLGTARTALFNWLFAKKEGGTFLLRIEDT
CEECCCCCCHHHHHEEEEEEEEECCCCCCEEEECHHHHHHHHHHHEECCCCEEEEEEECC
DIERSREEYINDIYDGLKWLGINWDESPTIQSERVNEHKQIIKTLVDKGFAYKCYASEAE
CHHHHHHHHHHHHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCH
LDEMRETQKRNGLAPKYDNRHRNLTPEQESEFIKLGRDPVIRFKISDEKLISWNDLIRGK
HHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEECHHHHHCCC
MTWSGKDLGGDMVIARRAPGNSIGDPLYNLVVVADDSAMKISHVIRGEDHLANTAKQILL
EEECCCCCCCCEEEEECCCCCCCCCCCEEEEEEECCCHHHHHHHHCCCHHHHHHHHHHHH
YEALDLNIPVFAHTPLILNSEGKKLSKRDGVTSISEFKKMGYTSEAMANYMTLLGWSVPE
HHHHCCCCCEEECCCEEECCCCCHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHHCCCCCC
GINERFNISEVSEIFSFKKVNKASAKFDWDKLNWLNSQVIHEMSAETLLENLEPLFKENG
CCCCCCCHHHHHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
WHLPSHEWGINLVGLIGPSMVLINDGVDQAKPFFEEQELSDDGKKQLEIKEAAVILKFIL
CCCCCCCCCCEEEEECCCCEEEEECCCHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHHHH
EKLADSDAASFSKEKALDLIDQATKSCKVKKGVVMKSLRAALFGTLNGPDLIQSWVLLSR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FSKDRARISRLI
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA