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The map label for this gene is 124025073

Identifier: 124025073

GI number: 124025073

Start: 332724

End: 334217

Strand: Reverse

Name: 124025073

Synonym: NATL1_03601

Alternate gene names: NA

Gene position: 334217-332724 (Counterclockwise)

Preceding gene: 124025074

Following gene: 124025070

Centisome position: 17.92

GC content: 35.27

Gene sequence:

>1494_bases
ATGGCAGTTAGTTATTTAAAAAGAGAAACATCACCAAAGCAAACAATCTTCAACAAAGAAGATTGGTCCAGTGCATATTG
CAATGTTGAAAAAGAATTAGATCACGTTCAACTCAAGCTTGTAAAAGGATCTATTCCTGAACAAATTTCTGGTACCTTTT
ATCGAAACGGGCCAGGTAGATTAGAAAGAGGGGGAAGATGGGTCCATCATCCATTTGATGGAGATGGCATGATTGCTGCC
TTCAAATTTGACAATGGAAAAATAAACCTGACGAATCGTTTTGTTCGCACAAAGGAATGGACAGAAGAAGAAAAATCCCA
AAAATTTCTATATAGAGGTGTATTTGGAACTCAAAAAGAAGGAGGAGTGTTAGCTAATGCTTTTGATGTAAGGCTAAAAA
ATATTGCCAATACACACGTAATAAAACTTGGAGATGATCTACTAGCTTTATGGGAAGCATCTAGTCCATATTCACTTAAT
CCAAATACCCTTGAGACCAAAGGTTTATCAAATTTAAAAGGAGTTTTAAAAAAAGGTGAAGCATTTAGTGCTCATCCACG
ATTTGACCCTGGCCATCATCAAAGTCAAAGAATGGTCACTTTTGGGGTATCTACAGGTCCTAAAAGCACAATAAGATTGA
TGGAATTTTCCACAAAGGGAGAAAATATTGGTTCTCTTTTAAGTGATAGAAAAGATTCTTTTAATGGATTTGCGTTCTTG
CATGATTTTGCCATAACTCCAAACTGGGCAATATTTCTGCAAAATGCTATTAGTTTTAATCCTCTTCCTTTTCTTCTTGG
ACAAAAAGGAGCCGCACAATGTTTAGCCTCTAAAAGTGATGGAACTCCAAAATTTTTATTAATTCCAAGGGACTCTGGCA
AGTTTGCTGGTCAACCTCCAAAATCAGTTGATGCTCCAAAGGGTTTTGTTTTTCATCATCTAAATGCATGGGAAGATAAT
GAAAAAATCAATATTGAAAGTATTTTTTATGATGATTTTCCGAGCATTGGACCCGAAGATAATTTTAGAGAAATTGATTT
TGATCTTTTACCAGAAGGAATTTTGAAAAGAAGTGAAATCAATCCCATAGAAAATACATTTACCTGCTCAACAATAAGCA
ATCAATGTTGTGAATTTGCAATGGTTAATCCTCATTTTGAAGGATTAAAGGCCCGCTTTAGTTGGATGGCAACTGCAGAA
GAAAAAGAGGGGAATGGGCCACTTCAAGCCATAAAAAAAATCGATTTATCTAATAATAAAGAGATAAGTTGGAGTGCGGC
TCCAAGAGGTTTTGTAAGTGAACCTATATTTATTCCATCTCAAGAATCAAAGTCTGAAGAAGACAATGGATGGGTTGTTG
CATTGGTTTGGAATAGTATTAGATCGGGAACTGATTTAATAATTCTTGATTCTAAAGATCTGACTGAAAAAGCTATTCTT
GAAGTTCCAATATCAATTCCACATGGATTACACGGAAGCTGGGTTGAAAATTAA

Upstream 100 bases:

>100_bases
GCTGGTCAATAAAAACTCCATATTTAAATAAATCATTTGAGCTTGAACTCGCAAAAGTGAGAAGTTTAAGCGAAAATCAA
GTTTGAATTCAAGAAAAATA

Downstream 100 bases:

>100_bases
AAACTAAAAGTTTTCAAGAAATCAAAAACTACTTTTTCATTGAACCCAATAGTTGTTTTAATTTTGGTATGAGCAATTTG
AATGCATTACCCCTGTGACC

Product: retinal pigment epithelial membrane protein

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MAVSYLKRETSPKQTIFNKEDWSSAYCNVEKELDHVQLKLVKGSIPEQISGTFYRNGPGRLERGGRWVHHPFDGDGMIAA
FKFDNGKINLTNRFVRTKEWTEEEKSQKFLYRGVFGTQKEGGVLANAFDVRLKNIANTHVIKLGDDLLALWEASSPYSLN
PNTLETKGLSNLKGVLKKGEAFSAHPRFDPGHHQSQRMVTFGVSTGPKSTIRLMEFSTKGENIGSLLSDRKDSFNGFAFL
HDFAITPNWAIFLQNAISFNPLPFLLGQKGAAQCLASKSDGTPKFLLIPRDSGKFAGQPPKSVDAPKGFVFHHLNAWEDN
EKINIESIFYDDFPSIGPEDNFREIDFDLLPEGILKRSEINPIENTFTCSTISNQCCEFAMVNPHFEGLKARFSWMATAE
EKEGNGPLQAIKKIDLSNNKEISWSAAPRGFVSEPIFIPSQESKSEEDNGWVVALVWNSIRSGTDLIILDSKDLTEKAIL
EVPISIPHGLHGSWVEN

Sequences:

>Translated_497_residues
MAVSYLKRETSPKQTIFNKEDWSSAYCNVEKELDHVQLKLVKGSIPEQISGTFYRNGPGRLERGGRWVHHPFDGDGMIAA
FKFDNGKINLTNRFVRTKEWTEEEKSQKFLYRGVFGTQKEGGVLANAFDVRLKNIANTHVIKLGDDLLALWEASSPYSLN
PNTLETKGLSNLKGVLKKGEAFSAHPRFDPGHHQSQRMVTFGVSTGPKSTIRLMEFSTKGENIGSLLSDRKDSFNGFAFL
HDFAITPNWAIFLQNAISFNPLPFLLGQKGAAQCLASKSDGTPKFLLIPRDSGKFAGQPPKSVDAPKGFVFHHLNAWEDN
EKINIESIFYDDFPSIGPEDNFREIDFDLLPEGILKRSEINPIENTFTCSTISNQCCEFAMVNPHFEGLKARFSWMATAE
EKEGNGPLQAIKKIDLSNNKEISWSAAPRGFVSEPIFIPSQESKSEEDNGWVVALVWNSIRSGTDLIILDSKDLTEKAIL
EVPISIPHGLHGSWVEN
>Mature_496_residues
AVSYLKRETSPKQTIFNKEDWSSAYCNVEKELDHVQLKLVKGSIPEQISGTFYRNGPGRLERGGRWVHHPFDGDGMIAAF
KFDNGKINLTNRFVRTKEWTEEEKSQKFLYRGVFGTQKEGGVLANAFDVRLKNIANTHVIKLGDDLLALWEASSPYSLNP
NTLETKGLSNLKGVLKKGEAFSAHPRFDPGHHQSQRMVTFGVSTGPKSTIRLMEFSTKGENIGSLLSDRKDSFNGFAFLH
DFAITPNWAIFLQNAISFNPLPFLLGQKGAAQCLASKSDGTPKFLLIPRDSGKFAGQPPKSVDAPKGFVFHHLNAWEDNE
KINIESIFYDDFPSIGPEDNFREIDFDLLPEGILKRSEINPIENTFTCSTISNQCCEFAMVNPHFEGLKARFSWMATAEE
KEGNGPLQAIKKIDLSNNKEISWSAAPRGFVSEPIFIPSQESKSEEDNGWVVALVWNSIRSGTDLIILDSKDLTEKAILE
VPISIPHGLHGSWVEN

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

Organism=Homo sapiens, GI74027270, Length=528, Percent_Identity=22.9166666666667, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71998242, Length=513, Percent_Identity=23.1968810916179, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI32564756, Length=540, Percent_Identity=22.4074074074074, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24646669, Length=306, Percent_Identity=23.8562091503268, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 55572; Mature: 55441

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSYLKRETSPKQTIFNKEDWSSAYCNVEKELDHVQLKLVKGSIPEQISGTFYRNGPGR
CCCHHHHHCCCCHHHCCCCCCCCHHHCCHHHCCCEEEEEEECCCCCHHHCCCEEECCCCC
LERGGRWVHHPFDGDGMIAAFKFDNGKINLTNRFVRTKEWTEEEKSQKFLYRGVFGTQKE
CCCCCEEEECCCCCCCEEEEEEECCCEEEEEHHEEECCHHHHHHHHHHHHHHCCCCCCCC
GGVLANAFDVRLKNIANTHVIKLGDDLLALWEASSPYSLNPNTLETKGLSNLKGVLKKGE
CCEEEEHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCC
AFSAHPRFDPGHHQSQRMVTFGVSTGPKSTIRLMEFSTKGENIGSLLSDRKDSFNGFAFL
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEE
HDFAITPNWAIFLQNAISFNPLPFLLGQKGAAQCLASKSDGTPKFLLIPRDSGKFAGQPP
EEEECCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC
KSVDAPKGFVFHHLNAWEDNEKINIESIFYDDFPSIGPEDNFREIDFDLLPEGILKRSEI
CCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCEECCHHHCCHHHHHCCCC
NPIENTFTCSTISNQCCEFAMVNPHFEGLKARFSWMATAEEKEGNGPLQAIKKIDLSNNK
CCCCCCEEEHHHHHHCCEEEECCCCHHHHHHHHHEEEECCCCCCCCHHHHHHHCCCCCCC
EISWSAAPRGFVSEPIFIPSQESKSEEDNGWVVALVWNSIRSGTDLIILDSKDLTEKAIL
EEEECCCCCCCCCCCEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCEEE
EVPISIPHGLHGSWVEN
EECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVSYLKRETSPKQTIFNKEDWSSAYCNVEKELDHVQLKLVKGSIPEQISGTFYRNGPGR
CCHHHHHCCCCHHHCCCCCCCCHHHCCHHHCCCEEEEEEECCCCCHHHCCCEEECCCCC
LERGGRWVHHPFDGDGMIAAFKFDNGKINLTNRFVRTKEWTEEEKSQKFLYRGVFGTQKE
CCCCCEEEECCCCCCCEEEEEEECCCEEEEEHHEEECCHHHHHHHHHHHHHHCCCCCCCC
GGVLANAFDVRLKNIANTHVIKLGDDLLALWEASSPYSLNPNTLETKGLSNLKGVLKKGE
CCEEEEHHHHHHHHCCCEEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHCCC
AFSAHPRFDPGHHQSQRMVTFGVSTGPKSTIRLMEFSTKGENIGSLLSDRKDSFNGFAFL
CCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCHHHHHHHCCCCCCCCEEEE
HDFAITPNWAIFLQNAISFNPLPFLLGQKGAAQCLASKSDGTPKFLLIPRDSGKFAGQPP
EEEECCCCCEEEEECCCCCCCCHHHHCCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC
KSVDAPKGFVFHHLNAWEDNEKINIESIFYDDFPSIGPEDNFREIDFDLLPEGILKRSEI
CCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCEECCHHHCCHHHHHCCCC
NPIENTFTCSTISNQCCEFAMVNPHFEGLKARFSWMATAEEKEGNGPLQAIKKIDLSNNK
CCCCCCEEEHHHHHHCCEEEECCCCHHHHHHHHHEEEECCCCCCCCHHHHHHHCCCCCCC
EISWSAAPRGFVSEPIFIPSQESKSEEDNGWVVALVWNSIRSGTDLIILDSKDLTEKAIL
EEEECCCCCCCCCCCEECCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCCCCCCEEE
EVPISIPHGLHGSWVEN
EECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]