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The map label for this gene is cobS [H]

Identifier: 124025062

GI number: 124025062

Start: 322880

End: 323461

Strand: Reverse

Name: cobS [H]

Synonym: NATL1_03491

Alternate gene names: 124025062

Gene position: 323461-322880 (Counterclockwise)

Preceding gene: 124025065

Following gene: 124025060

Centisome position: 17.35

GC content: 32.47

Gene sequence:

>582_bases
TTGATATCTATTGCAATTAGCATCTGCATAACTGGTGGACTTCATATTGACGGGTTAATGGATACAGCTGATGGGATTGG
TGCAGGGCCATCAAAGCGAATTGAGGCAATGAAAGACAGCCGAGTTGGAGCTATAGGTGTGCAAAGTTTAGTAATTATTT
TAGTCCTTCAAATAGCCGCAATTATCAAACTTGGTTTTTATGCTCCTTTTGCTTTTCCAATAGCTGCCTTCTGGGGACGA
TTCTCCCAAATAGTTGCTATTGAAAATTATGAATACATTCTCAATAAAGAATTTGGTTCCATTCATCAAAAATACTGGAG
AGGCATCTCCCAAGAAATAAGACCTTCATTAATAATAATTTGTATTGGCATAATATTATTTTTATCTACAACTAACTTAA
ATTTATCTAATACATTATTATTGATATATTGCATTTCTTCAGGATTAACAACTTCAATATTAATACCACACTTTATAAAC
AAATTCCTAGGAGGACATAATGGTGATAGTTATGGAGCAGGTTTAGTGATAACGGAAACTACTAATTTATTACTATTAAG
TATCATTTTGATTCCAAATTAA

Upstream 100 bases:

>100_bases
TGCTCGCTTTGCACCTTTAATAGGACTTTTAATCGGCTTTTTACAATCTTTTTTTTGGCTTGTCTTTAACTACTTTGATT
GGCCCCATACTTCTATCGCC

Downstream 100 bases:

>100_bases
AAACGAAAGCATTCCCTGATTCAGGCAAAGAGTTTTTAAATTGACTTGGATCAACTAATAACTGTAAATCTCCACCTAGT
TGTCTAGCCAAATCTCTTGC

Product: cobalamin-5-phosphate synthase CobS

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MISIAISICITGGLHIDGLMDTADGIGAGPSKRIEAMKDSRVGAIGVQSLVIILVLQIAAIIKLGFYAPFAFPIAAFWGR
FSQIVAIENYEYILNKEFGSIHQKYWRGISQEIRPSLIIICIGIILFLSTTNLNLSNTLLLIYCISSGLTTSILIPHFIN
KFLGGHNGDSYGAGLVITETTNLLLLSIILIPN

Sequences:

>Translated_193_residues
MISIAISICITGGLHIDGLMDTADGIGAGPSKRIEAMKDSRVGAIGVQSLVIILVLQIAAIIKLGFYAPFAFPIAAFWGR
FSQIVAIENYEYILNKEFGSIHQKYWRGISQEIRPSLIIICIGIILFLSTTNLNLSNTLLLIYCISSGLTTSILIPHFIN
KFLGGHNGDSYGAGLVITETTNLLLLSIILIPN
>Mature_193_residues
MISIAISICITGGLHIDGLMDTADGIGAGPSKRIEAMKDSRVGAIGVQSLVIILVLQIAAIIKLGFYAPFAFPIAAFWGR
FSQIVAIENYEYILNKEFGSIHQKYWRGISQEIRPSLIIICIGIILFLSTTNLNLSNTLLLIYCISSGLTTSILIPHFIN
KFLGGHNGDSYGAGLVITETTNLLLLSIILIPN

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 20893; Mature: 20893

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIAISICITGGLHIDGLMDTADGIGAGPSKRIEAMKDSRVGAIGVQSLVIILVLQIAA
CEEEEEEEEEECCEEECCHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IIKLGFYAPFAFPIAAFWGRFSQIVAIENYEYILNKEFGSIHQKYWRGISQEIRPSLIII
HHHHHHCCHHHHHHHHHHHHHHHEEEECCHHHHHCCHHHHHHHHHHHCHHHHHCHHHHHH
CIGIILFLSTTNLNLSNTLLLIYCISSGLTTSILIPHFINKFLGGHNGDSYGAGLVITET
HHHHHHHHHCCCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
TNLLLLSIILIPN
HHHHHHHHEECCC
>Mature Secondary Structure
MISIAISICITGGLHIDGLMDTADGIGAGPSKRIEAMKDSRVGAIGVQSLVIILVLQIAA
CEEEEEEEEEECCEEECCHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IIKLGFYAPFAFPIAAFWGRFSQIVAIENYEYILNKEFGSIHQKYWRGISQEIRPSLIII
HHHHHHCCHHHHHHHHHHHHHHHEEEECCHHHHHCCHHHHHHHHHHHCHHHHHCHHHHHH
CIGIILFLSTTNLNLSNTLLLIYCISSGLTTSILIPHFINKFLGGHNGDSYGAGLVITET
HHHHHHHHHCCCCCCCHHEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEECC
TNLLLLSIILIPN
HHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]