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The map label for this gene is phnE [H]

Identifier: 124023133

GI number: 124023133

Start: 1223319

End: 1224884

Strand: Reverse

Name: phnE [H]

Synonym: P9303_14291

Alternate gene names: 124023133

Gene position: 1224884-1223319 (Counterclockwise)

Preceding gene: 124023134

Following gene: 124023132

Centisome position: 45.66

GC content: 47.06

Gene sequence:

>1566_bases
ATGAGTCGCCTCAGTCCAACTCCTCCAATCCTGAGCCTTTTGCCAGCACTGGCTTTATTGCCTGTACTTATTTCTATCCT
CATAAGTTTCCACAATGGTGGAGTCACTCTCCTATGGGAGTTTATATGCGCTGCCGTTCATCCATCTATTCAAGCAGAGG
TCGTTCAAAGTGTATTTAGAGGAATACAGATCACTTTGGCCACAGCTCTTCTTAGCTGGTCAATCAGTTCAATATTTGGA
ATTTGTCTTGGCATAGCCAGCGCCAGGGTGGTATGGGAGACCTTTTTAGGAAAAAGCTGGCCAGCGATTGTGATTCGCAG
ACTTTTGGCAATACCGCGAGCAATTCATGAACTGCTCTGGGGTTTGTTGTTACTTCAAATGGTTGGACTGAGCCCTTGGG
TAGCAATTGTTGCAATCGTCATTCCTTACACCTCACTTGTTGCTCGAGTCGTAAGCGATCAACTTGACACCCAGGATCGT
CGAGCCTTAAGGGGCTTAAAACAGGCTGGGGCGGGCTCCTCCTCCGCATTGATTACAGCTCTTGGCCCTGCGATGGGGCC
TGTCTTATTGAGTTATGTCGGATATCGACTTGAATGTGCATTAAGAGGGGCAACCGTGTTGGGGGTTTTTGGATTAGGTG
GTATTGGGACTGAGCTTCAACTGGCACTTCAATCTCTTCAATTCAAGGACGTATGGACAGCTTTATGGTTACTGGCAGCA
GTGATGTTTAGTCTTGAGCAGATATTAGGTTTGATACGTGGATATTTCATATTATCGAAAAATGGAATATGGCAAATTGC
CACAATGTTGATTGTCTTATTGATTCTTTGCGCTCTAAGTATTCCCTGGCTCAATGCAGTTGATATTGATCTAGGAGAAC
CTCTTCACTGGAGTCCCCTTCCATTACCAACTGTTGATGAGCTAATAAATGCTATTAATGCTCTCCCATGGATCCAATTA
ATCAGCAGCACGATGTTGCTAACAGTGTTAGCAGCAGGAATAGCTATCGGAACACCGCCACTAGCAATGATGCTTTGGCC
AAGCCGACTGGGAATAGCAATACAAAGAATCATCTGGGGACTGCTAAGACTGATGCCAACCCCTCTTACTGCTCTATTTT
TGCTGCTCTTCAGCAAACCGAGTGTGGCTGTTGCTGCTCTTGCACTTGGAGCCCACAACATTGGAGTGATGGGCCGCCTC
TTAAAAGAAGGACTTGACCAACAAAGCGACAACAATTTGAAAGCCCTAAGCGCGACTGGCGCCGGAGCACGATCGGCTTG
GCTCTATGGATGCTTTAGCGGCCAAAGCCGTAGCTATTTAGCCTATGCCGCTTACAGAACGGACGTGATTTTGCGTGAAA
CCGTGGTAGTTGGCATGGTTGGAGGCACTGGACTGGGTTGGCAGTTGATCGAATCTCTTGGTTCGTTCAACTGGGCACAG
GTCAGTGTCCTAATCGCGGTGTTCACAGCTCTGACCTTGATCGGAGAATCAATCACTGATCGCTTGCGATATCACTGGAC
TGGTTCAACTAAAAAGCTACCTATGGTTGTAGACCTACAAAGCTGA

Upstream 100 bases:

>100_bases
ACTTTGACAGGGTAATCGCACTTAAGTCTGGGAGAGTTGTCTTTGATCAACCTGCTGAATGTCTTGATGAAGAAGCCTTA
GCCTTGATCTACGGATCTGC

Downstream 100 bases:

>100_bases
TCAGGTTCCACTTGTCGATGTGATGGCCAATACATACAAGAGACTCGTTGTCATTGGTGACAGTGGTGTTTATGGATGGG
GAGATCGAGAGGGGGGCGGA

Product: phosphonate ABC transporter

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 520

Protein sequence:

>521_residues
MSRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSVFRGIQITLATALLSWSISSIFG
ICLGIASARVVWETFLGKSWPAIVIRRLLAIPRAIHELLWGLLLLQMVGLSPWVAIVAIVIPYTSLVARVVSDQLDTQDR
RALRGLKQAGAGSSSALITALGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTELQLALQSLQFKDVWTALWLLAA
VMFSLEQILGLIRGYFILSKNGIWQIATMLIVLLILCALSIPWLNAVDIDLGEPLHWSPLPLPTVDELINAINALPWIQL
ISSTMLLTVLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWGLLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRL
LKEGLDQQSDNNLKALSATGAGARSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS

Sequences:

>Translated_521_residues
MSRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSVFRGIQITLATALLSWSISSIFG
ICLGIASARVVWETFLGKSWPAIVIRRLLAIPRAIHELLWGLLLLQMVGLSPWVAIVAIVIPYTSLVARVVSDQLDTQDR
RALRGLKQAGAGSSSALITALGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTELQLALQSLQFKDVWTALWLLAA
VMFSLEQILGLIRGYFILSKNGIWQIATMLIVLLILCALSIPWLNAVDIDLGEPLHWSPLPLPTVDELINAINALPWIQL
ISSTMLLTVLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWGLLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRL
LKEGLDQQSDNNLKALSATGAGARSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS
>Mature_520_residues
SRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSVFRGIQITLATALLSWSISSIFGI
CLGIASARVVWETFLGKSWPAIVIRRLLAIPRAIHELLWGLLLLQMVGLSPWVAIVAIVIPYTSLVARVVSDQLDTQDRR
ALRGLKQAGAGSSSALITALGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTELQLALQSLQFKDVWTALWLLAAV
MFSLEQILGLIRGYFILSKNGIWQIATMLIVLLILCALSIPWLNAVDIDLGEPLHWSPLPLPTVDELINAINALPWIQLI
SSTMLLTVLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWGLLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRLL
KEGLDQQSDNNLKALSATGAGARSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQV
SVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 56080; Mature: 55948

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50928 ABC_TM1 ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSVFR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
GIQITLATALLSWSISSIFGICLGIASARVVWETFLGKSWPAIVIRRLLAIPRAIHELLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLLLLQMVGLSPWVAIVAIVIPYTSLVARVVSDQLDTQDRRALRGLKQAGAGSSSALITA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
LGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTELQLALQSLQFKDVWTALWLLAA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VMFSLEQILGLIRGYFILSKNGIWQIATMLIVLLILCALSIPWLNAVDIDLGEPLHWSPL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
PLPTVDELINAINALPWIQLISSTMLLTVLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHH
LLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRLLKEGLDQQSDNNLKALSATG
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCC
AGARSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
CCCCHHHEEEEECCCCCCEEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHH
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECC
>Mature Secondary Structure 
SRLSPTPPILSLLPALALLPVLISILISFHNGGVTLLWEFICAAVHPSIQAEVVQSVFR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
GIQITLATALLSWSISSIFGICLGIASARVVWETFLGKSWPAIVIRRLLAIPRAIHELLW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GLLLLQMVGLSPWVAIVAIVIPYTSLVARVVSDQLDTQDRRALRGLKQAGAGSSSALITA
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
LGPAMGPVLLSYVGYRLECALRGATVLGVFGLGGIGTELQLALQSLQFKDVWTALWLLAA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VMFSLEQILGLIRGYFILSKNGIWQIATMLIVLLILCALSIPWLNAVDIDLGEPLHWSPL
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
PLPTVDELINAINALPWIQLISSTMLLTVLAAGIAIGTPPLAMMLWPSRLGIAIQRIIWG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHHHHHHHHHHHH
LLRLMPTPLTALFLLLFSKPSVAVAALALGAHNIGVMGRLLKEGLDQQSDNNLKALSATG
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCEEEECCC
AGARSAWLYGCFSGQSRSYLAYAAYRTDVILRETVVVGMVGGTGLGWQLIESLGSFNWAQ
CCCCHHHEEEEECCCCCCEEEEHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCHHH
VSVLIAVFTALTLIGESITDRLRYHWTGSTKKLPMVVDLQS
HHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]