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The map label for this gene is glnB [H]

Identifier: 124022177

GI number: 124022177

Start: 468962

End: 469300

Strand: Reverse

Name: glnB [H]

Synonym: P9303_04671

Alternate gene names: 124022177

Gene position: 469300-468962 (Counterclockwise)

Preceding gene: 124022178

Following gene: 124022175

Centisome position: 17.49

GC content: 51.62

Gene sequence:

>339_bases
ATGAAGAAAGTCGAGGCGATTGTCCGTCCCTTCAAGCTTGAGGACGTCAAGCTTGCATTAGTCAATGCCGAGATCATCGG
CATGACTGTGAGTGAAGTGAGAGGCTTCGGACGCCAGAAAGGCCAAGTTGAGCGCTATAGGGGCTCGGAATTTACCGTTG
AATTCCTGCAGAAGTTAAAGGTCGAAGTGGTCGTCGACGACGACAAGGTGGAAGCTGTCGTGAACGCCATTGCTGAAGCT
GCCAAAACAGGCGAAATCGGTGACGGCAAGATTTTCATCTCTCCAGTGGATTCCGTAGTACGTATCCGTACCGGTGATCG
TGACAGCAAAGCCCTCTAG

Upstream 100 bases:

>100_bases
AAGAACGCAGGGATGAGCCTCGGTCATCTATCTGCCCTATCAGTTCCAGGCCGTTTCTGACAAAATTCCTTTATCTCTGA
ACGAGCAACATCAAGCCGTC

Downstream 100 bases:

>100_bases
GCCAACGTCTTAGCCACAAGTCTCTCAAGAACTGGAAAATCTGCGAGGGTCTCGTCACCCAGCCAGAGCAGGTATTCATG
ATTACCAGCCGGTCCAGTGA

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: PII signal transducing protein [H]

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKKVEAIVRPFKLEDVKLALVNAEIIGMTVSEVRGFGRQKGQVERYRGSEFTVEFLQKLKVEVVVDDDKVEAVVNAIAEA
AKTGEIGDGKIFISPVDSVVRIRTGDRDSKAL

Sequences:

>Translated_112_residues
MKKVEAIVRPFKLEDVKLALVNAEIIGMTVSEVRGFGRQKGQVERYRGSEFTVEFLQKLKVEVVVDDDKVEAVVNAIAEA
AKTGEIGDGKIFISPVDSVVRIRTGDRDSKAL
>Mature_112_residues
MKKVEAIVRPFKLEDVKLALVNAEIIGMTVSEVRGFGRQKGQVERYRGSEFTVEFLQKLKVEVVVDDDKVEAVVNAIAEA
AKTGEIGDGKIFISPVDSVVRIRTGDRDSKAL

Specific function: P-II indirectly controls the transcription of the GS gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is phosphorylated, these events are reversed. In nitrogen-limiting condi

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=57.1428571428571, Blast_Score=133, Evalue=2e-33,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=53.5714285714286, Blast_Score=128, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12348; Mature: 12348

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVEAIVRPFKLEDVKLALVNAEIIGMTVSEVRGFGRQKGQVERYRGSEFTVEFLQKLK
CCCHHHHHCCCCCCCEEEEEECHHEEEEEHHHHHCCCCCCCCHHHHCCCCHHHHHHHHCE
VEVVVDDDKVEAVVNAIAEAAKTGEIGDGKIFISPVDSVVRIRTGDRDSKAL
EEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCC
>Mature Secondary Structure
MKKVEAIVRPFKLEDVKLALVNAEIIGMTVSEVRGFGRQKGQVERYRGSEFTVEFLQKLK
CCCHHHHHCCCCCCCEEEEEECHHEEEEEHHHHHCCCCCCCCHHHHCCCCHHHHHHHHCE
VEVVVDDDKVEAVVNAIAEAAKTGEIGDGKIFISPVDSVVRIRTGDRDSKAL
EEEEECCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA