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The map label for this gene is grpE

Identifier: 124021735

GI number: 124021735

Start: 24985

End: 25698

Strand: Direct

Name: grpE

Synonym: P9303_00201

Alternate gene names: 124021735

Gene position: 24985-25698 (Clockwise)

Preceding gene: 124021731

Following gene: 124021736

Centisome position: 0.93

GC content: 53.5

Gene sequence:

>714_bases
ATGAGTGGCGACGCCCCTACCCCAGCTCACGATCCTGCTGCTGAGGGTTTAGAGGCTTCCGTGCCTCTGGAATCAGTGGC
ATCGATGAATTCCGACGAGGGTCAGCCTTCAGCTCAATCTGCACCATTAGCGGATAATGAGGCTCGCTTACAGCAGTTGG
AGCAGGAGCACAGCAGCCTGCGGGAAGAGCACGAAACTCTTCGTAGTCAGTACATGCGGATTGCTGCAGATTTCGATAAT
TTCCGCAAACGTCAGAGTCGCGATCAGGACGATCTGCGATTGCAGCTCATCTGTACTACTTTGAGTGAAATTCTACCGGT
GGTGGACAATTTTGAGCGAGCTCGTCAGCAATTAGAGCCTCAGGGAGAGGAGGCCCAGGCTCTTCATCGCAGTTATCAGG
GTCTTTATAAACAGCTTGTTGAGGTGCTCAAGCAATTGGGCGTTGCTTCAATGCGGGTTGTTGGACAGGCGTTTGATCCC
ACGCTCCATGAGGCTGTTTCAAGAGAGCCCAGTGAGGAGCATCCAGAGGATGTAGTCACAGAGGAACTTCAGCGTGGATA
TCACCTCAATGGCAGGGTGCTTCGCCATGCTCTTGTGAAGGTGTCTATGGGACCAGGCCCGCAGAGTGGCGCCTCTCCTT
CTTCAGCTCAATCCAATGATGACTCCACTGCAACTTTTCAAGGTGAGGCAGATCCTGCCGAGCCAGGGGTCTGA

Upstream 100 bases:

>100_bases
CACGAAGAAGAAATGAAATTGGAGGTGCGGGCTTCCGCCGCTTGTGGGAGGTGTGCTACTCCCATCAACATGTCTAGACG
TGAATTTGCAAACGGTCAGC

Downstream 100 bases:

>100_bases
TTGCATGCTGTTGCCCTTTAGGGTGAAGGAGTGTTGACGAGCTGATGGCTGATTATTACGACCTGCTAGGGGTCAGCAAA
GATGCTGATGGCGATACCCT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MSGDAPTPAHDPAAEGLEASVPLESVASMNSDEGQPSAQSAPLADNEARLQQLEQEHSSLREEHETLRSQYMRIAADFDN
FRKRQSRDQDDLRLQLICTTLSEILPVVDNFERARQQLEPQGEEAQALHRSYQGLYKQLVEVLKQLGVASMRVVGQAFDP
TLHEAVSREPSEEHPEDVVTEELQRGYHLNGRVLRHALVKVSMGPGPQSGASPSSAQSNDDSTATFQGEADPAEPGV

Sequences:

>Translated_237_residues
MSGDAPTPAHDPAAEGLEASVPLESVASMNSDEGQPSAQSAPLADNEARLQQLEQEHSSLREEHETLRSQYMRIAADFDN
FRKRQSRDQDDLRLQLICTTLSEILPVVDNFERARQQLEPQGEEAQALHRSYQGLYKQLVEVLKQLGVASMRVVGQAFDP
TLHEAVSREPSEEHPEDVVTEELQRGYHLNGRVLRHALVKVSMGPGPQSGASPSSAQSNDDSTATFQGEADPAEPGV
>Mature_236_residues
SGDAPTPAHDPAAEGLEASVPLESVASMNSDEGQPSAQSAPLADNEARLQQLEQEHSSLREEHETLRSQYMRIAADFDNF
RKRQSRDQDDLRLQLICTTLSEILPVVDNFERARQQLEPQGEEAQALHRSYQGLYKQLVEVLKQLGVASMRVVGQAFDPT
LHEAVSREPSEEHPEDVVTEELQRGYHLNGRVLRHALVKVSMGPGPQSGASPSSAQSNDDSTATFQGEADPAEPGV

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Escherichia coli, GI1788967, Length=135, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653432, Length=193, Percent_Identity=27.979274611399, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_PROM3 (A2C5L7)

Other databases:

- EMBL:   CP000554
- RefSeq:   YP_001016042.1
- ProteinModelPortal:   A2C5L7
- SMR:   A2C5L7
- STRING:   A2C5L7
- GeneID:   4777023
- GenomeReviews:   CP000554_GR
- KEGG:   pmf:P9303_00201
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NDARLEQ
- ProtClustDB:   PRK14143
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 26003; Mature: 25872

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGDAPTPAHDPAAEGLEASVPLESVASMNSDEGQPSAQSAPLADNEARLQQLEQEHSSL
CCCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
REEHETLRSQYMRIAADFDNFRKRQSRDQDDLRLQLICTTLSEILPVVDNFERARQQLEP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGEEAQALHRSYQGLYKQLVEVLKQLGVASMRVVGQAFDPTLHEAVSREPSEEHPEDVVT
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHH
EELQRGYHLNGRVLRHALVKVSMGPGPQSGASPSSAQSNDDSTATFQGEADPAEPGV
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
SGDAPTPAHDPAAEGLEASVPLESVASMNSDEGQPSAQSAPLADNEARLQQLEQEHSSL
CCCCCCCCCCCHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
REEHETLRSQYMRIAADFDNFRKRQSRDQDDLRLQLICTTLSEILPVVDNFERARQQLEP
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
QGEEAQALHRSYQGLYKQLVEVLKQLGVASMRVVGQAFDPTLHEAVSREPSEEHPEDVVT
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHCCCCCCCCHHHHHH
EELQRGYHLNGRVLRHALVKVSMGPGPQSGASPSSAQSNDDSTATFQGEADPAEPGV
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA