Click here to switch to the map view.

The map label for this gene is pntA [H]

Identifier: 123968855

GI number: 123968855

Start: 1100247

End: 1101377

Strand: Reverse

Name: pntA [H]

Synonym: A9601_13221

Alternate gene names: 123968855

Gene position: 1101377-1100247 (Counterclockwise)

Preceding gene: 123968859

Following gene: 123968854

Centisome position: 65.96

GC content: 36.96

Gene sequence:

>1131_bases
TTGACAAAGATACTTATTCCTTCCGAAACAAGCTCTGGTGAAAGGAGAGTTTCAGCCACACCAGAAGCTGTAAAGAAATT
AAATAGCCTTGGATGTGATGTTTATATTGAAAGTTCAGCAGGAAAATTATCAGGATTTAGTGACTTATCATATGAAGAAT
CGGGAGGAACAATAATAAACAACTTAGATCAAAAAATTTGGGGTGAAGCTGACTTAGTATTTTGTGTTCGAACTCCATCA
GAGGATAATTTAACTAAGTTAAAGAAAGGTGCTGTTCTTCTTGGTCTTCTTAACCCATATGGTAATAAAGAGCTTCTAAG
GATTATAAATAGTAATAAGATTTCAGCTTTATCACTAGAGTTGCTTCCGAGGATTAGTAGAGCTCAATCTTCTGATGTTC
TTTCTTCACAGGCTAATATTGCTGGATATAAAGCAGTTCTTTTGGCTGCAAGTGAGTTAGATAGATATTTTCCAATGCTT
ATGACTGCTGCAGGGACTGTCCAACCTGCAAAAGTAGTGGTTCTTGGTGGAGGAGTTGCAGGATTGCAGGCAGTTGCGAC
GGCGAAAAGACTTGGAGCAATAGTATTTGTGTCTGATATTAGACCTGCTGTTAAAGAACAAGTAGAGTCCCTCGGAGCAA
GATTTATAGAACTTCCTGAAGTAGAGGAAAAGCCAGGAGAGGCAGGAGGTTATGCAAAAGCTGTAACACCAGAATTCCTC
TCAAAACAGAAGGCAACTTTAACTAAATATTTATCTGAAGCTGATGTTGCTATATGTACTGCGCAAGTTCTAGGTAAAAA
GGCCCCTGTTTTAATAGACTCACCCATGATTGAAAAAATGAGGCCTGGAGCAGTAGTTATTGATTTAGCAGTTTCTCAGG
GAGGTAACTGCGAAGGAACAAAATCTAATGAAACTATTATCAAAGATGGGGTAAAACTTATAGGAGCAGGCGAATTACCC
TCTTCAGTTCCTTATGATGCAAGTTCACTTTATGCTAAGAATTTAACATCTTTGATTACACCATTTATAAAAGATGGTGT
AATTAAATTAGATAAAGAGGATGAACTCATTTCTGGATGTTTATTAAGCGATGAAGGAGTTGTTCTTCAAAATAAAGTTT
TTGAAAATTGA

Upstream 100 bases:

>100_bases
GATAGCTATTTTTTACAGAAAAGAAAATATATTTTTAACAAAAATCCAAGACTTTTGACTTTCTTCAATTTATATTTAGT
ATGTTAAAAAAATAAATAAA

Downstream 100 bases:

>100_bases
GGTTTTAAAATTATGTCTTTTATAAGTCTTCTTTGGGTTCTTTTACTTGGTAGTTTATTAGGTCTCGAGTTAATTGGAAA
AGTTCCTCCTACTCTTCACA

Product: putative nicotinamide nucleotide transhydrogenase, subunit alpha 1 (A1)

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTKILIPSETSSGERRVSATPEAVKKLNSLGCDVYIESSAGKLSGFSDLSYEESGGTIINNLDQKIWGEADLVFCVRTPS
EDNLTKLKKGAVLLGLLNPYGNKELLRIINSNKISALSLELLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPML
MTAAGTVQPAKVVVLGGGVAGLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEVEEKPGEAGGYAKAVTPEFL
SKQKATLTKYLSEADVAICTAQVLGKKAPVLIDSPMIEKMRPGAVVIDLAVSQGGNCEGTKSNETIIKDGVKLIGAGELP
SSVPYDASSLYAKNLTSLITPFIKDGVIKLDKEDELISGCLLSDEGVVLQNKVFEN

Sequences:

>Translated_376_residues
MTKILIPSETSSGERRVSATPEAVKKLNSLGCDVYIESSAGKLSGFSDLSYEESGGTIINNLDQKIWGEADLVFCVRTPS
EDNLTKLKKGAVLLGLLNPYGNKELLRIINSNKISALSLELLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPML
MTAAGTVQPAKVVVLGGGVAGLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEVEEKPGEAGGYAKAVTPEFL
SKQKATLTKYLSEADVAICTAQVLGKKAPVLIDSPMIEKMRPGAVVIDLAVSQGGNCEGTKSNETIIKDGVKLIGAGELP
SSVPYDASSLYAKNLTSLITPFIKDGVIKLDKEDELISGCLLSDEGVVLQNKVFEN
>Mature_375_residues
TKILIPSETSSGERRVSATPEAVKKLNSLGCDVYIESSAGKLSGFSDLSYEESGGTIINNLDQKIWGEADLVFCVRTPSE
DNLTKLKKGAVLLGLLNPYGNKELLRIINSNKISALSLELLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPMLM
TAAGTVQPAKVVVLGGGVAGLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEVEEKPGEAGGYAKAVTPEFLS
KQKATLTKYLSEADVAICTAQVLGKKAPVLIDSPMIEKMRPGAVVIDLAVSQGGNCEGTKSNETIIKDGVKLIGAGELPS
SVPYDASSLYAKNLTSLITPFIKDGVIKLDKEDELISGCLLSDEGVVLQNKVFEN

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=342, Percent_Identity=42.3976608187134, Blast_Score=238, Evalue=6e-63,
Organism=Homo sapiens, GI122939153, Length=342, Percent_Identity=42.3976608187134, Blast_Score=238, Evalue=6e-63,
Organism=Escherichia coli, GI1787887, Length=367, Percent_Identity=40.8719346049046, Blast_Score=272, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17550456, Length=364, Percent_Identity=41.4835164835165, Blast_Score=277, Evalue=6e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39860; Mature: 39729

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKILIPSETSSGERRVSATPEAVKKLNSLGCDVYIESSAGKLSGFSDLSYEESGGTIIN
CCEEEECCCCCCCCEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHH
NLDQKIWGEADLVFCVRTPSEDNLTKLKKGAVLLGLLNPYGNKELLRIINSNKISALSLE
HHHHHHCCCCEEEEEEECCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCEEEHHH
LLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPMLMTAAGTVQPAKVVVLGGGVA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
GLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEVEEKPGEAGGYAKAVTPEFL
HHHHHHHHHHHCEEEEEHHCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHH
SKQKATLTKYLSEADVAICTAQVLGKKAPVLIDSPMIEKMRPGAVVIDLAVSQGGNCEGT
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEECCHHHHHCCCCEEEEEEEECCCCCCCCC
KSNETIIKDGVKLIGAGELPSSVPYDASSLYAKNLTSLITPFIKDGVIKLDKEDELISGC
CCCCHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHCE
LLSDEGVVLQNKVFEN
EECCCCEEECCCCCCC
>Mature Secondary Structure 
TKILIPSETSSGERRVSATPEAVKKLNSLGCDVYIESSAGKLSGFSDLSYEESGGTIIN
CEEEECCCCCCCCEEECCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCHHHH
NLDQKIWGEADLVFCVRTPSEDNLTKLKKGAVLLGLLNPYGNKELLRIINSNKISALSLE
HHHHHHCCCCEEEEEEECCCCCCHHHHHCCEEEEEECCCCCCHHHHHHHCCCCCEEEHHH
LLPRISRAQSSDVLSSQANIAGYKAVLLAASELDRYFPMLMTAAGTVQPAKVVVLGGGVA
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHH
GLQAVATAKRLGAIVFVSDIRPAVKEQVESLGARFIELPEVEEKPGEAGGYAKAVTPEFL
HHHHHHHHHHHCEEEEEHHCCHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCHHHH
SKQKATLTKYLSEADVAICTAQVLGKKAPVLIDSPMIEKMRPGAVVIDLAVSQGGNCEGT
HHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEECCHHHHHCCCCEEEEEEEECCCCCCCCC
KSNETIIKDGVKLIGAGELPSSVPYDASSLYAKNLTSLITPFIKDGVIKLDKEDELISGC
CCCCHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHCE
LLSDEGVVLQNKVFEN
EECCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]