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The map label for this gene is lipA2 [H]

Identifier: 123968735

GI number: 123968735

Start: 1018371

End: 1019270

Strand: Reverse

Name: lipA2 [H]

Synonym: A9601_12021

Alternate gene names: 123968735

Gene position: 1019270-1018371 (Counterclockwise)

Preceding gene: 123968736

Following gene: 123968728

Centisome position: 61.04

GC content: 32.56

Gene sequence:

>900_bases
ATGAGAAACAATAATCTTATCAAGAAAGAAAAAATCTTAAGGCTTCCCTCTTGGATAAAATTTCCCATTAGTAAATCATC
AGAGTTAGAAAAAATACAAACACTCATCAAAAAATCAAATATTCACACTATTTGTGAAGAAGCAAGATGTCCAAATAGAG
CAGAATGTTATGCCTCAGGAACAGCCACCTTCTTACTGGGTGGATCGATATGCTCTCGTTCTTGTGCTTTTTGTCAGGTA
AATAAAGGTAGACCTAGTTCTATTAATATTGATGAATGCACTCAAGTCGCTGAAGCAGTAAAAGTACTAAATTTGAAATA
TGTTGTTTTGACATCTGTAGCTAGAGATGATCTCCCTGATCATGGTGCAAATTTATTTATATCTACAATTGATGAGATTA
GAAAAATTGATTCAACAATTAAAATAGAGGTTTTAACTCCTGATTTATGGGGTGGGGGCAAAAATTTTGATGAAACTAAC
AACCTTCAGACTGAGAGATTAAAAATGATCTTAGAAAAAAATCCAACATGCTTTAATCATAATCTTGAAACTGTTGAAAG
ATTGCAAAAAGAAGTTAGGAGAGGTGCAAATTACAAAAAATCCCTTAGCTTATTAAAAAAGTCAAAAGACATTGCCCCTC
ATATTCAAACTAAATCAGGCATCATGTTAGGTCTTGGTGAAACCTTGGATGAAATAAAAAATACAATTTATGATCTTAAA
AAAATAGATTGTGATCAAATTACAATTGGCCAATATTTAAGGCCCTCATTCAATCATTTGGCAGTTAAGAAATATTGGGA
TCCATCAGAGTTCGAATATTTATATCGCTTTTCCAAGGAATTAGGATTCAAGAAAGTATCTTCTGGGCCCTTAGTTAGAA
GTAGTTATCATGCGGGTTGA

Upstream 100 bases:

>100_bases
GAGGATTGCATATGGGCTCCCAATGGGGAGTGAACTTGAATATGTGGATGAAGTTACACTTGCGAGAGCCTTAGAGGGAA
GAACAAAATTAAATTAGATA

Downstream 100 bases:

>100_bases
CTTCCCTCAATTAAAAAAAGTTTCTTTTCAAGTTTTTTCAATATGGAAGTACATTCCCCGTTCATAAGTGTACCCGCACC
ACCCCAAACCAATCTTAATA

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 2; Lipoate synthase 2; Lipoic acid synthase 2; Sulfur insertion protein lipA2 [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MRNNNLIKKEKILRLPSWIKFPISKSSELEKIQTLIKKSNIHTICEEARCPNRAECYASGTATFLLGGSICSRSCAFCQV
NKGRPSSINIDECTQVAEAVKVLNLKYVVLTSVARDDLPDHGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETN
NLQTERLKMILEKNPTCFNHNLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIYDLK
KIDCDQITIGQYLRPSFNHLAVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG

Sequences:

>Translated_299_residues
MRNNNLIKKEKILRLPSWIKFPISKSSELEKIQTLIKKSNIHTICEEARCPNRAECYASGTATFLLGGSICSRSCAFCQV
NKGRPSSINIDECTQVAEAVKVLNLKYVVLTSVARDDLPDHGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETN
NLQTERLKMILEKNPTCFNHNLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIYDLK
KIDCDQITIGQYLRPSFNHLAVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG
>Mature_299_residues
MRNNNLIKKEKILRLPSWIKFPISKSSELEKIQTLIKKSNIHTICEEARCPNRAECYASGTATFLLGGSICSRSCAFCQV
NKGRPSSINIDECTQVAEAVKVLNLKYVVLTSVARDDLPDHGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETN
NLQTERLKMILEKNPTCFNHNLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIYDLK
KIDCDQITIGQYLRPSFNHLAVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=299, Percent_Identity=40.133779264214, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI37577164, Length=262, Percent_Identity=38.5496183206107, Blast_Score=186, Evalue=2e-47,
Organism=Escherichia coli, GI1786846, Length=296, Percent_Identity=42.9054054054054, Blast_Score=257, Evalue=6e-70,
Organism=Caenorhabditis elegans, GI32564533, Length=309, Percent_Identity=39.8058252427184, Blast_Score=208, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6324770, Length=297, Percent_Identity=42.0875420875421, Blast_Score=229, Evalue=4e-61,
Organism=Drosophila melanogaster, GI221513272, Length=298, Percent_Identity=42.6174496644295, Blast_Score=232, Evalue=2e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33920; Mature: 33920

Theoretical pI: Translated: 9.33; Mature: 9.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNNNLIKKEKILRLPSWIKFPISKSSELEKIQTLIKKSNIHTICEEARCPNRAECYASG
CCCCCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHEEECC
TATFLLGGSICSRSCAFCQVNKGRPSSINIDECTQVAEAVKVLNLKYVVLTSVARDDLPD
CEEEEECCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETNNLQTERLKMILEKNPTCFNH
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
NLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIYDLK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
KIDCDQITIGQYLRPSFNHLAVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG
HCCCCCEEHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MRNNNLIKKEKILRLPSWIKFPISKSSELEKIQTLIKKSNIHTICEEARCPNRAECYASG
CCCCCCCHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHEEECC
TATFLLGGSICSRSCAFCQVNKGRPSSINIDECTQVAEAVKVLNLKYVVLTSVARDDLPD
CEEEEECCHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HGANLFISTIDEIRKIDSTIKIEVLTPDLWGGGKNFDETNNLQTERLKMILEKNPTCFNH
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
NLETVERLQKEVRRGANYKKSLSLLKKSKDIAPHIQTKSGIMLGLGETLDEIKNTIYDLK
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHH
KIDCDQITIGQYLRPSFNHLAVKKYWDPSEFEYLYRFSKELGFKKVSSGPLVRSSYHAG
HCCCCCEEHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]