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The map label for this gene is prsA [H]

Identifier: 123968719

GI number: 123968719

Start: 1000177

End: 1001172

Strand: Reverse

Name: prsA [H]

Synonym: A9601_11861

Alternate gene names: 123968719

Gene position: 1001172-1000177 (Counterclockwise)

Preceding gene: 123968721

Following gene: 123968716

Centisome position: 59.95

GC content: 36.14

Gene sequence:

>996_bases
GTGACAAGTTTTATCACGGCAATGCAGAGTAAAGAATCGAACTTTAATCTAACTAATAGTAGATTAAGATTAGTAAGTGG
GACAACAAACCCAAAGTTAGCTGAAGAAATTGCATCATACTTAGGGATTGAAAATGTACCTTTAATATCTAAAAGATTTG
CAGATGGAGAACTTTATGTTCAGATTCAGCAATCTATTAGAGGTTGTGATGTATTCCTTATACAACCTACATGCGCTCCT
GTAAACGATAGTTTAATGGAACTAATGATCATGATTGACGCTTGCAAAAGAGCCTCTGCAAGACAAATAACGGCTGTAAT
TCCCTATTTTGGATATGCGAGGGCAGATAGGAAAACCTCAGGAAGAGAGTCTATAACTGCAAAATTAACTGCCAATTTAC
TAGAGAAATCAGGAGTTGATAGAGTTTTAGCAATGGACTTACACTCAGCTCAAATACAAGGCTACTTCGATATACCATGC
GATCATATTTACGGTTCACCTGTATTAATTGATTATCTAGAATCTCTAGATTTAGAAGAAATAGTTGTAGTCTCTCCTGA
TGTAGGCGGGGTAGCAAGAGCAAGAGCATTCGCCAAATTAATGAATGATGCGCCTTTGGCTATAATTGATAAAAGGAGAT
CTGCGCATAATATCGCTGAAAGTCTAACGGTTATCGGTGAAGTTAAAGGTAAAACTGCTATTCTTATAGACGACATGATA
GATACGGGAGGCACAATTTGTTCTGGAGCAAATTTGCTAAAGAAAGAAGGAGCTAATAGGATATTTGCTTGTGCCTCACA
TGCTGTATTTTCTCCTCCTTCTTATGAAAGATTAAGTTCTAAGAATCTTTTCGAACAAGTTATTGTGACTAACAGCATAC
CAGTTAAACATAAAGATAATTTTCCACAATTAAAAGTTCTTTCTGTCGCAAATATGCTTGGGGAAGCCATTTGGAGAATT
CACGAAGAAAGTTCTGTTAGTTCTATGTTTAGATAA

Upstream 100 bases:

>100_bases
TTGCTTAATTGATGTGACTTTTGAAAATATCTAGTCATTATCTTTTGCGAAATTCACATAAACGACTACGATGATAATAT
AGTTTTATGGTGCAAATTTC

Downstream 100 bases:

>100_bases
AAAAATTTATTTTTTACAAACCTTGTAATTTCTTGATTCTCTCAGTTTCAATCTTAAATTGTTTTTCGATCTTTTCAGGA
ACATTATCTGGGCACACTTT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MTSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMIDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLIDYLESLDLEEIVVVSPDVGGVARARAFAKLMNDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
DTGGTICSGANLLKKEGANRIFACASHAVFSPPSYERLSSKNLFEQVIVTNSIPVKHKDNFPQLKVLSVANMLGEAIWRI
HEESSVSSMFR

Sequences:

>Translated_331_residues
MTSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYVQIQQSIRGCDVFLIQPTCAP
VNDSLMELMIMIDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPC
DHIYGSPVLIDYLESLDLEEIVVVSPDVGGVARARAFAKLMNDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
DTGGTICSGANLLKKEGANRIFACASHAVFSPPSYERLSSKNLFEQVIVTNSIPVKHKDNFPQLKVLSVANMLGEAIWRI
HEESSVSSMFR
>Mature_330_residues
TSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYVQIQQSIRGCDVFLIQPTCAPV
NDSLMELMIMIDACKRASARQITAVIPYFGYARADRKTSGRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCD
HIYGSPVLIDYLESLDLEEIVVVSPDVGGVARARAFAKLMNDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMID
TGGTICSGANLLKKEGANRIFACASHAVFSPPSYERLSSKNLFEQVIVTNSIPVKHKDNFPQLKVLSVANMLGEAIWRIH
EESSVSSMFR

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=312, Percent_Identity=41.9871794871795, Blast_Score=267, Evalue=1e-71,
Organism=Homo sapiens, GI4506129, Length=310, Percent_Identity=41.9354838709677, Blast_Score=266, Evalue=3e-71,
Organism=Homo sapiens, GI28557709, Length=312, Percent_Identity=41.9871794871795, Blast_Score=265, Evalue=6e-71,
Organism=Homo sapiens, GI84875539, Length=313, Percent_Identity=41.85303514377, Blast_Score=260, Evalue=1e-69,
Organism=Homo sapiens, GI4506133, Length=356, Percent_Identity=35.6741573033708, Blast_Score=184, Evalue=8e-47,
Organism=Homo sapiens, GI194018537, Length=333, Percent_Identity=33.6336336336336, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=28.4722222222222, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=28.4722222222222, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=28.4722222222222, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=28.4722222222222, Blast_Score=79, Evalue=4e-15,
Organism=Escherichia coli, GI1787458, Length=311, Percent_Identity=49.8392282958199, Blast_Score=327, Evalue=7e-91,
Organism=Caenorhabditis elegans, GI17554702, Length=328, Percent_Identity=41.7682926829268, Blast_Score=260, Evalue=8e-70,
Organism=Caenorhabditis elegans, GI71989924, Length=328, Percent_Identity=41.7682926829268, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI25149168, Length=312, Percent_Identity=42.3076923076923, Blast_Score=256, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=42.5806451612903, Blast_Score=254, Evalue=5e-68,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=30.1169590643275, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319403, Length=328, Percent_Identity=38.4146341463415, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6320946, Length=316, Percent_Identity=40.8227848101266, Blast_Score=234, Evalue=1e-62,
Organism=Saccharomyces cerevisiae, GI6321776, Length=317, Percent_Identity=39.1167192429022, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=41.6243654822335, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324511, Length=98, Percent_Identity=37.7551020408163, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355239, Length=329, Percent_Identity=41.3373860182371, Blast_Score=269, Evalue=2e-72,
Organism=Drosophila melanogaster, GI45551540, Length=352, Percent_Identity=38.6363636363636, Blast_Score=255, Evalue=3e-68,
Organism=Drosophila melanogaster, GI24651458, Length=361, Percent_Identity=30.4709141274238, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI24651456, Length=361, Percent_Identity=30.4709141274238, Blast_Score=179, Evalue=3e-45,
Organism=Drosophila melanogaster, GI281362873, Length=361, Percent_Identity=30.4709141274238, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651454, Length=361, Percent_Identity=30.4709141274238, Blast_Score=178, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24651462, Length=186, Percent_Identity=37.0967741935484, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI24651464, Length=186, Percent_Identity=37.0967741935484, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45552010, Length=186, Percent_Identity=37.0967741935484, Blast_Score=127, Evalue=9e-30,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 36316; Mature: 36185

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYV
CCHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHCCCCEEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMIDACKRASARQITAVIPYFGYARADRKTS
EEHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCCCCC
GRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLIDYLESLDLEE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCEEECCCHHHHCCCHHHHHHHHHCCCCE
IVVVSPDVGGVARARAFAKLMNDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHEECCCCEEEEEECHH
DTGGTICSGANLLKKEGANRIFACASHAVFSPPSYERLSSKNLFEQVIVTNSIPVKHKDN
CCCCCEECCCHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FPQLKVLSVANMLGEAIWRIHEESSVSSMFR
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure 
TSFITAMQSKESNFNLTNSRLRLVSGTTNPKLAEEIASYLGIENVPLISKRFADGELYV
CHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHCCCCEEEE
QIQQSIRGCDVFLIQPTCAPVNDSLMELMIMIDACKRASARQITAVIPYFGYARADRKTS
EEHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCHHHEEEEECCCCCHHCCCCCC
GRESITAKLTANLLEKSGVDRVLAMDLHSAQIQGYFDIPCDHIYGSPVLIDYLESLDLEE
CCHHHHHHHHHHHHHHCCCCEEEEEEHHHHHCCEEECCCHHHHCCCHHHHHHHHHCCCCE
IVVVSPDVGGVARARAFAKLMNDAPLAIIDKRRSAHNIAESLTVIGEVKGKTAILIDDMI
EEEECCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHEECCCCEEEEEECHH
DTGGTICSGANLLKKEGANRIFACASHAVFSPPSYERLSSKNLFEQVIVTNSIPVKHKDN
CCCCCEECCCHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
FPQLKVLSVANMLGEAIWRIHEESSVSSMFR
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]