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The map label for this gene is cocE [H]

Identifier: 123968176

GI number: 123968176

Start: 561350

End: 562930

Strand: Reverse

Name: cocE [H]

Synonym: A9601_06411

Alternate gene names: 123968176

Gene position: 562930-561350 (Counterclockwise)

Preceding gene: 123968177

Following gene: 123968175

Centisome position: 33.71

GC content: 33.84

Gene sequence:

>1581_bases
ATGTCTGGTTCTAGATGGTTTGACAAGTCTCTAATACTTAGAGATGGAGTAAAACTTATATCAAGGATTTGGTTACCTAA
TAGCAATGGGCCATGGCCTGCATTATTAATGAGACAACCTTATGGCAGGGAAATTGCTTCAACTATCACCTATTCTCATC
CTGAATGGTGGGCTTCCAAAGGGTATATGGTCATAATTCAAGACGTTAGAGGTATGGGTTCTTCTGAAGGAGTCTTTAAT
GGTTTTTCTCAAGAAGCTAGCGATACTTCAGAAACTCATGAATGGGTAAGGTCTCTAAAAGAATGTAATGGGAAACTTGG
CTTATATGGTTTTTCATATCAAGGATTTACTCAACTAACCGGTGAATTATATTCAAAGCCGCCCGATTGTTTATCTCCAG
CAATGACTGGGATGAATATTAAGGATCATTGGTGCTCAGATGGAGGAGCATATTGGTGGCATAACAATATTGCATGGGGA
CTTCAAATTGCGGCACTAAAAATGAAAAAAGAAAATAAATTGCTTGAGTGGGGAAAGATAAGATTAGCCCTAGAAAATAA
AAGTTATTTAAAGGAAGGAATTGAAATTTTAAAAAAATATGATCCTAATAGCTTTGTTTTGGAATGGCTTAAAAATTTAA
ATAATGCTCACCCATTTGAAGAATTTAAACCAATTTCAACATGGATTAAACAACCTATGTTAATTATTGGAGGACTTTGG
GATCCACATTTAAAAGGTGCCTTTGATCTTTATCAACAATCTAAAGAAGCGGGTGGAAGCCCAGAGATTATTATTGGGAA
TGCAACACATCTAAATTGGTGGGAGGGATCACAAGAATCTTTATTAAATTTTTTTGATAAACATTTAAAATTAGATGAAA
ATTTTAATTCTAAGAATTTAAAAGACGAGAAAAAAATATGGAATATTTCATTAAATAAATGGGAAGATTTAGATAATAAA
TTTAATCCTGAATTTATTTTTGGACTCAAAAGCGATGGTACCGCAAATGTAGAGGTTGAAGATGGAAGTCTGACCATAAA
TTCAAAAGGATCAGGATGGTTCACAATTGTTCATGACCCATGGAGACCTACTCCATCAGACGGTGGACATTTAGGTCAAA
ATCCAGGAAAGTTTAATAGAAGTATTATTGATAAAAGACTGGATGTAGGTGTTTTTCAAACCAATTCTTTTGAAGAAGAT
CAATATTTAACAGGAGTACCCACATTAGAAATCCAGGTAAAAAGTGATCAGCCCAATTTCGATATCTGCCTTGCTTTATC
TCTAGTTGAAGAAGGTAATGAAAAGGTGAATCAATTTTCAACCGGATTCTTAAGGGTTAAAAACTCCAAAATAAATGAAG
AATGCATTTATCAAATCACAATGCAACCAACAAATATTTGTTTGATTAAAGATAGCAAGCTTCGCTTATCTATATCTGCT
GCAGCTTATCCCGCTATTGGAGTTAATCCGGGATTTGGGGATGGAAATATTGGATCTCCTTCAGCAAATCATAGAGTTAT
TACTCTAAGTTTTAGCCTTGATAAAACATTTATGAAAATGACCCCTTTTTTTTATAAATAA

Upstream 100 bases:

>100_bases
GAGTATGTATCAAACAAAAAAAAGTTAACTCCCGTTAACGCAGAGATCTTTTCTATTTACGTCATTGGGCTGCACTGTCC
AGACATTATCTAAAGCCTAA

Downstream 100 bases:

>100_bases
TTAATTTTTTGGTTAATCATTTGATATTATTAATCGTTAATATCTACCAGTCATGATCGAGACAATTCAAGCAGACTGGA
TTAAATCAGAAGCTATCAAC

Product: acyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSGSRWFDKSLILRDGVKLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASKGYMVIIQDVRGMGSSEGVFN
GFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGFTQLTGELYSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWG
LQIAALKMKKENKLLEWGKIRLALENKSYLKEGIEILKKYDPNSFVLEWLKNLNNAHPFEEFKPISTWIKQPMLIIGGLW
DPHLKGAFDLYQQSKEAGGSPEIIIGNATHLNWWEGSQESLLNFFDKHLKLDENFNSKNLKDEKKIWNISLNKWEDLDNK
FNPEFIFGLKSDGTANVEVEDGSLTINSKGSGWFTIVHDPWRPTPSDGGHLGQNPGKFNRSIIDKRLDVGVFQTNSFEED
QYLTGVPTLEIQVKSDQPNFDICLALSLVEEGNEKVNQFSTGFLRVKNSKINEECIYQITMQPTNICLIKDSKLRLSISA
AAYPAIGVNPGFGDGNIGSPSANHRVITLSFSLDKTFMKMTPFFYK

Sequences:

>Translated_526_residues
MSGSRWFDKSLILRDGVKLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASKGYMVIIQDVRGMGSSEGVFN
GFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGFTQLTGELYSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWG
LQIAALKMKKENKLLEWGKIRLALENKSYLKEGIEILKKYDPNSFVLEWLKNLNNAHPFEEFKPISTWIKQPMLIIGGLW
DPHLKGAFDLYQQSKEAGGSPEIIIGNATHLNWWEGSQESLLNFFDKHLKLDENFNSKNLKDEKKIWNISLNKWEDLDNK
FNPEFIFGLKSDGTANVEVEDGSLTINSKGSGWFTIVHDPWRPTPSDGGHLGQNPGKFNRSIIDKRLDVGVFQTNSFEED
QYLTGVPTLEIQVKSDQPNFDICLALSLVEEGNEKVNQFSTGFLRVKNSKINEECIYQITMQPTNICLIKDSKLRLSISA
AAYPAIGVNPGFGDGNIGSPSANHRVITLSFSLDKTFMKMTPFFYK
>Mature_525_residues
SGSRWFDKSLILRDGVKLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASKGYMVIIQDVRGMGSSEGVFNG
FSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGFTQLTGELYSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGL
QIAALKMKKENKLLEWGKIRLALENKSYLKEGIEILKKYDPNSFVLEWLKNLNNAHPFEEFKPISTWIKQPMLIIGGLWD
PHLKGAFDLYQQSKEAGGSPEIIIGNATHLNWWEGSQESLLNFFDKHLKLDENFNSKNLKDEKKIWNISLNKWEDLDNKF
NPEFIFGLKSDGTANVEVEDGSLTINSKGSGWFTIVHDPWRPTPSDGGHLGQNPGKFNRSIIDKRLDVGVFQTNSFEEDQ
YLTGVPTLEIQVKSDQPNFDICLALSLVEEGNEKVNQFSTGFLRVKNSKINEECIYQITMQPTNICLIKDSKLRLSISAA
AYPAIGVNPGFGDGNIGSPSANHRVITLSFSLDKTFMKMTPFFYK

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 59637; Mature: 59506

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSRWFDKSLILRDGVKLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASK
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHEECCCCHHHCCC
GYMVIIQDVRGMGSSEGVFNGFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGFTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
GELYSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGLQIAALKMKKENKLLEWGKI
HHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEEECCCCCCEEECEE
RLALENKSYLKEGIEILKKYDPNSFVLEWLKNLNNAHPFEEFKPISTWIKQPMLIIGGLW
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCC
DPHLKGAFDLYQQSKEAGGSPEIIIGNATHLNWWEGSQESLLNFFDKHLKLDENFNSKNL
CCCHHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCC
KDEKKIWNISLNKWEDLDNKFNPEFIFGLKSDGTANVEVEDGSLTINSKGSGWFTIVHDP
CCCCEEEEEECCHHHCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEEECC
WRPTPSDGGHLGQNPGKFNRSIIDKRLDVGVFQTNSFEEDQYLTGVPTLEIQVKSDQPNF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEECCCEEEEEEECCCCCC
DICLALSLVEEGNEKVNQFSTGFLRVKNSKINEECIYQITMQPTNICLIKDSKLRLSISA
EEEEEEEHHHCCCHHHHHHCCCEEEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEEEE
AAYPAIGVNPGFGDGNIGSPSANHRVITLSFSLDKTFMKMTPFFYK
CCCCEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCCC
>Mature Secondary Structure 
SGSRWFDKSLILRDGVKLISRIWLPNSNGPWPALLMRQPYGREIASTITYSHPEWWASK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHEECCCCHHHCCC
GYMVIIQDVRGMGSSEGVFNGFSQEASDTSETHEWVRSLKECNGKLGLYGFSYQGFTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHH
GELYSKPPDCLSPAMTGMNIKDHWCSDGGAYWWHNNIAWGLQIAALKMKKENKLLEWGKI
HHHHCCCCHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEEEEEEEEECCCCCCEEECEE
RLALENKSYLKEGIEILKKYDPNSFVLEWLKNLNNAHPFEEFKPISTWIKQPMLIIGGLW
EEEECCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCC
DPHLKGAFDLYQQSKEAGGSPEIIIGNATHLNWWEGSQESLLNFFDKHLKLDENFNSKNL
CCCHHHHHHHHHHHHHCCCCCCEEECCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCC
KDEKKIWNISLNKWEDLDNKFNPEFIFGLKSDGTANVEVEDGSLTINSKGSGWFTIVHDP
CCCCEEEEEECCHHHCCCCCCCCEEEEEECCCCEEEEEEECCEEEEECCCCEEEEEEECC
WRPTPSDGGHLGQNPGKFNRSIIDKRLDVGVFQTNSFEEDQYLTGVPTLEIQVKSDQPNF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEECCCCCCCCEEECCCEEEEEEECCCCCC
DICLALSLVEEGNEKVNQFSTGFLRVKNSKINEECIYQITMQPTNICLIKDSKLRLSISA
EEEEEEEHHHCCCHHHHHHCCCEEEEECCCCCCEEEEEEEECCCEEEEEECCCEEEEEEE
AAYPAIGVNPGFGDGNIGSPSANHRVITLSFSLDKTFMKMTPFFYK
CCCCEECCCCCCCCCCCCCCCCCCEEEEEEECCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]