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The map label for this gene is gloB [H]

Identifier: 123968150

GI number: 123968150

Start: 536512

End: 537423

Strand: Reverse

Name: gloB [H]

Synonym: A9601_06151

Alternate gene names: 123968150

Gene position: 537423-536512 (Counterclockwise)

Preceding gene: 123968154

Following gene: 123968149

Centisome position: 32.18

GC content: 29.93

Gene sequence:

>912_bases
TTGCAATTTGATTCAAAGGTTAATGATCTATTATTTTCTTCCAACGCATTAGAGAAATCTAACCCAGCTTTTTTAAAAGT
TGAAATTGAATCTTTTAACAGAGCTCCTTTTGGTAAAGCTATAGTAATCATAGATCAATTTCTTCCAAGGATTCTTCATT
CTAATTTAACAATGGAATTTAATAAAGCTCGAAATATAATCGGACTTAGAGTTTTAAGTGATAACGTCATATGGTTGATG
GCAAAAGATAAATCCGTTGTAGTTATAGATCCATCTGTACACGAACCAGTTATTAGATATATAAATGAAAACAATTTTCA
CTTAGAAGCTATTTTGCAAACTCATCATCATTCAGACCATATTGGAGGGACGAAGTCTCTTATTGAAAAATGGCCAAATG
TAAAGGTGATTGCTTCCTCCAAAGAAAAAAAGCGAATCCCTTTTCAAAATGTATCTGTTGAAGATGGAGAAACTTTAAAC
ATTTTAGGGGAAGAAGTAAAAATAATTGAAGTATTAGGACATACAAGCTCACATATTGCCTTCTTTTTGAATGGGGAAAA
TCCTGTTCTTTTTATTGGTGACACATTGTTTTCTGGAGGATGTGGAAGAATTTTTGAGGGAACTTATCAACAAATGTATT
CTTCACTAGAAAGAATCAAATCTTTACCAAAAAATACTCTCATATATTGTGCACATGAATATACAAAGGCAAATATATTG
TGGGCATTGAATCTAAAGCCAAAAGATCAAGATATAAAAAATAAACTTTCGGAAGTTGAAAAAAAACTTTCACTTAACGA
ATTGACAATTCCATTTTTACTAGATGAAGAGATGAAAATAAACCTTTTCTTAAGAGCAAAAAATTTAGAAGAATTTACTT
TTTTAAGAGCAAATAAAGATTTATGGGTTTAA

Upstream 100 bases:

>100_bases
TTCTCGTAAAACGTCATACCCAACAATACCCAAATCAGCCTGACCATAACTTACATAAACAGGAACATCTCCATTTCTTA
CTAATAGAGCTTTTGCCCGT

Downstream 100 bases:

>100_bases
ATAGATAGGTATCTTCCTAAACAAATGTCCTCAAAACAAGTAATTAAAACATCAAATGCTCCAGATCCAGTAGGACCTTA
TAATCAAGCAATAAAAGCTG

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQFDSKVNDLLFSSNALEKSNPAFLKVEIESFNRAPFGKAIVIIDQFLPRILHSNLTMEFNKARNIIGLRVLSDNVIWLM
AKDKSVVVIDPSVHEPVIRYINENNFHLEAILQTHHHSDHIGGTKSLIEKWPNVKVIASSKEKKRIPFQNVSVEDGETLN
ILGEEVKIIEVLGHTSSHIAFFLNGENPVLFIGDTLFSGGCGRIFEGTYQQMYSSLERIKSLPKNTLIYCAHEYTKANIL
WALNLKPKDQDIKNKLSEVEKKLSLNELTIPFLLDEEMKINLFLRAKNLEEFTFLRANKDLWV

Sequences:

>Translated_303_residues
MQFDSKVNDLLFSSNALEKSNPAFLKVEIESFNRAPFGKAIVIIDQFLPRILHSNLTMEFNKARNIIGLRVLSDNVIWLM
AKDKSVVVIDPSVHEPVIRYINENNFHLEAILQTHHHSDHIGGTKSLIEKWPNVKVIASSKEKKRIPFQNVSVEDGETLN
ILGEEVKIIEVLGHTSSHIAFFLNGENPVLFIGDTLFSGGCGRIFEGTYQQMYSSLERIKSLPKNTLIYCAHEYTKANIL
WALNLKPKDQDIKNKLSEVEKKLSLNELTIPFLLDEEMKINLFLRAKNLEEFTFLRANKDLWV
>Mature_303_residues
MQFDSKVNDLLFSSNALEKSNPAFLKVEIESFNRAPFGKAIVIIDQFLPRILHSNLTMEFNKARNIIGLRVLSDNVIWLM
AKDKSVVVIDPSVHEPVIRYINENNFHLEAILQTHHHSDHIGGTKSLIEKWPNVKVIASSKEKKRIPFQNVSVEDGETLN
ILGEEVKIIEVLGHTSSHIAFFLNGENPVLFIGDTLFSGGCGRIFEGTYQQMYSSLERIKSLPKNTLIYCAHEYTKANIL
WALNLKPKDQDIKNKLSEVEKKLSLNELTIPFLLDEEMKINLFLRAKNLEEFTFLRANKDLWV

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538322, Length=264, Percent_Identity=32.5757575757576, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI94538320, Length=226, Percent_Identity=34.5132743362832, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI14150041, Length=221, Percent_Identity=33.0316742081448, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI116642887, Length=243, Percent_Identity=31.2757201646091, Blast_Score=121, Evalue=6e-28,
Organism=Homo sapiens, GI21703352, Length=243, Percent_Identity=31.2757201646091, Blast_Score=121, Evalue=7e-28,
Organism=Homo sapiens, GI46361987, Length=198, Percent_Identity=31.8181818181818, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1786406, Length=253, Percent_Identity=40.3162055335968, Blast_Score=185, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17536925, Length=231, Percent_Identity=32.9004329004329, Blast_Score=137, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17538952, Length=227, Percent_Identity=29.5154185022026, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320478, Length=230, Percent_Identity=33.0434782608696, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324614, Length=219, Percent_Identity=31.0502283105023, Blast_Score=79, Evalue=8e-16,
Organism=Drosophila melanogaster, GI21356335, Length=244, Percent_Identity=34.0163934426229, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24667703, Length=244, Percent_Identity=34.0163934426229, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI24667711, Length=242, Percent_Identity=33.8842975206612, Blast_Score=142, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 34675; Mature: 34675

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFDSKVNDLLFSSNALEKSNPAFLKVEIESFNRAPFGKAIVIIDQFLPRILHSNLTMEF
CCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEE
NKARNIIGLRVLSDNVIWLMAKDKSVVVIDPSVHEPVIRYINENNFHLEAILQTHHHSDH
CCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCC
IGGTKSLIEKWPNVKVIASSKEKKRIPFQNVSVEDGETLNILGEEVKIIEVLGHTSSHIA
CCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCEEE
FFLNGENPVLFIGDTLFSGGCGRIFEGTYQQMYSSLERIKSLPKNTLIYCAHEYTKANIL
EEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
WALNLKPKDQDIKNKLSEVEKKLSLNELTIPFLLDEEMKINLFLRAKNLEEFTFLRANKD
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEECCCCCEEEEECCCC
LWV
CCC
>Mature Secondary Structure
MQFDSKVNDLLFSSNALEKSNPAFLKVEIESFNRAPFGKAIVIIDQFLPRILHSNLTMEF
CCCCHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEHHHHHHHHHHCCCEEEE
NKARNIIGLRVLSDNVIWLMAKDKSVVVIDPSVHEPVIRYINENNFHLEAILQTHHHSDH
CCCCCEEEEEEECCCEEEEEECCCEEEEECCCCCHHHHHHHCCCCEEEEEEEECCCCCCC
IGGTKSLIEKWPNVKVIASSKEKKRIPFQNVSVEDGETLNILGEEVKIIEVLGHTSSHIA
CCCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCEEE
FFLNGENPVLFIGDTLFSGGCGRIFEGTYQQMYSSLERIKSLPKNTLIYCAHEYTKANIL
EEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEE
WALNLKPKDQDIKNKLSEVEKKLSLNELTIPFLLDEEMKINLFLRAKNLEEFTFLRANKD
EEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCEEEEEEEEECCCCCEEEEECCCC
LWV
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA