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The map label for this gene is ndhB [H]

Identifier: 123968027

GI number: 123968027

Start: 423947

End: 425467

Strand: Reverse

Name: ndhB [H]

Synonym: A9601_04901

Alternate gene names: 123968027

Gene position: 425467-423947 (Counterclockwise)

Preceding gene: 123968033

Following gene: 123968026

Centisome position: 25.48

GC content: 36.42

Gene sequence:

>1521_bases
GTGCCCAACGAAATCTTTACAATTAATTTAAATGCTCAAGCCATTATTCCAGAGGCTTTTATTTTACTAGGTATTGTTGG
AACACTTCTTGTAGATTTAGCTGGAGAAAAAACTGCATCGAAGTGGGCACCAATAATTTGCTATTTGTCAATCGGAAGCT
CTCTTGTTAGCTTGGCATTGCAGTGGAGTAATCCGGTAGAAAACGCATTCCTTGGATCCTTTAATTCAGATAATTTGGCA
ATCGCATTTAGAGCAATAATTTCTTTATCAACCTTGATTTCTTTACTTATAAGTTGGCGGTATACAGAACAAAGTGGAAG
CCCAATTGGAGAGTTTGCTGCGATAGTTCTTTCAGCCACACTTGGAGCAATGCTTTTATGTGGATCTACTGACCTTATTA
GTGTATTTATTTCTCTTGAAACTTTATCTGTAGCAAGCTACTTACTTTCTGGCTACCTCAAGAGAGATCCAAGAAGTTCA
GAAGCGGCCTTAAAATACCTCCTTGTCGGATCAGCTGCTGCTGCTGTCTATTTGTATGGATCCTCTTTTCTTTATGGATT
AAGTGGTTCAACAAACTTAGCGACAATAGGTTTAGAGATTATCAATAAGCCATCCTTCATAACTTCACTAGCACTTGTAT
TTGTCTTATCAACAGTTGCATTTAAAATTGCTGCTGTTCCCTTTCATCAATGGACTCCTGACGTATATGAGGGTTCACCT
ACTCCTGTAGTAGCTTTTTTATCTGTTGGTTCAAAAACAGCGGGCTTTGCATTTGCGATAAGAATATTAAGCACAACTTT
CTCTTCTTTTGACGAAGAATGGAAACTTTTATTTACCATTTTGGCAATATTGAGCATGGCTCTAGGAAATGTTGTAGCTC
TAGCTCAAACCTCAATGAAAAGGATGCTAGCTTACAGTTCTATTGGACAAGCAGGATTTGTAATGATTGGAATAGTATCT
GGCACACAAGATGGTTTATCAGCTGCTGTTTTATATTTGGCTGCATATTTATTTATGAATTTGGGTGCATTTTCTTGTGT
AATACTTTTCTCACTAAGAACTGGTTCTGACAGAATTCTTGATTACTCAGGACTTTACCAAAAAGATCCTCTCATTACAT
TAGGCTTAAGCCTTTGTCTTCTATCACTTGGAGGTTTACCTCCAATGTTGGGATTTTTTGGAAAAATATACTTGTTCTTT
GCAGGTTGGGCCAATCATCAATATCTACTAGTTATTGTTGGATTAGTAACTTCAGTTATATCTATTTATTACTACATTTC
AGTGATAAAAATGATGGTAGTTAAAGAACCACAGGAAGCTTCTGAAATAGTCAAATCATATCCTGAAATTAATTGGGGAA
TTGAAGGATTACCTCCCTTGAGAATTGCACTTTACACTTGCGTAGCGGTAACTGCTCTTGGAGGAATCCTATCTAATCCT
CTTTTTAAATTAGCCAATACAGCAGTTTCAGAAACTCCTTTTTTACAAGATATTATTGCTATAGCAAACAATATTTCCTA
G

Upstream 100 bases:

>100_bases
TTCTGAAAATTTCAAAAATCATACCCACAAATTATAATCAAGTTAAGATTAACTCCTAGACTCTTACAATCAAACTTCTA
ATTTAATCCAATTTAATAAA

Downstream 100 bases:

>100_bases
AAAAATTCTTCAATAAATGTCTAAGAAAGTCGCTAGTTTAGAAAATATATCTAAAACATATGGTAAAGAGGATCTAACTG
TCAAAGCCTTAGACAGCATA

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2 [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLVSLALQWSNPVENAFLGSFNSDNLA
IAFRAIISLSTLISLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVIVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIEGLPPLRIALYTCVAVTALGGILSNP
LFKLANTAVSETPFLQDIIAIANNIS

Sequences:

>Translated_506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLVSLALQWSNPVENAFLGSFNSDNLA
IAFRAIISLSTLISLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVIVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIEGLPPLRIALYTCVAVTALGGILSNP
LFKLANTAVSETPFLQDIIAIANNIS
>Mature_505_residues
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLVSLALQWSNPVENAFLGSFNSDNLAI
AFRAIISLSTLISLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSSE
AALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSPT
PVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVSG
TQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFA
GWANHQYLLVIVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIEGLPPLRIALYTCVAVTALGGILSNPL
FKLANTAVSETPFLQDIIAIANNIS

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=367, Percent_Identity=24.2506811989101, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI145693160, Length=386, Percent_Identity=33.419689119171, Blast_Score=209, Evalue=5e-55,
Organism=Escherichia coli, GI1788829, Length=446, Percent_Identity=24.2152466367713, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788831, Length=297, Percent_Identity=27.9461279461279, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788614, Length=431, Percent_Identity=24.8259860788863, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788613, Length=360, Percent_Identity=24.4444444444444, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.5.3

Molecular weight: Translated: 54141; Mature: 54010

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLVSLAL
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHH
QWSNPVENAFLGSFNSDNLAIAFRAIISLSTLISLLISWRYTEQSGSPIGEFAAIVLSAT
HCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAVYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEEEC
SSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRIL
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCEE
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVIVGLVTSVI
ECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIEGLPPLRIALYTCVAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCH
LFKLANTAVSETPFLQDIIAIANNIS
HHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPIICYLSIGSSLVSLAL
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHH
QWSNPVENAFLGSFNSDNLAIAFRAIISLSTLISLLISWRYTEQSGSPIGEFAAIVLSAT
HCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAVYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEEEC
SSFLYGLSGSTNLATIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCEEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRIL
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCEE
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVIVGLVTSVI
ECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEINWGIEGLPPLRIALYTCVAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCH
LFKLANTAVSETPFLQDIIAIANNIS
HHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA