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The map label for this gene is leuB [H]

Identifier: 123966030

GI number: 123966030

Start: 717472

End: 718545

Strand: Direct

Name: leuB [H]

Synonym: P9515_07951

Alternate gene names: 123966030

Gene position: 717472-718545 (Clockwise)

Preceding gene: 123966029

Following gene: 123966031

Centisome position: 42.1

GC content: 31.47

Gene sequence:

>1074_bases
ATGAAGAAATATAAAGTTGTTTTACTTTCTGGAGATGGTATAGGACCTGAAATATCAGAAATTTCTTCAGAAATATTAAA
AAAACTATCTCATAAATATTCTTTTGATATTGAGATTAAAGAAGAATATTTTGGTGGAATAGCTTACGAAAAGTATAAAG
ATCCAGCTCCGAAAGCGACATTAGATCAGTGCAAATCAAGTGATGCAGTTCTTTTAGCTTGTGTGGGAGATTTGAAATAT
GATTCTTTACCAAGAGAATTACGACCAGAAAGTGGTTTACTTAAATTAAGATCTGCGCTGAATTTATTCGCTAATATAAG
ACCGGTAAAAATAAGAAAATCACTTTTAGATGCCAGCTCTTTAAAAAAAGAAATCATTGAAGATGTAGATTTAGTCGTAG
TAAGAGAATTAATAAGTGGAATATATTTTGGACAGCCCAGGGGACAAATTACTAACACTAAAGTTAAAAAAGCTTTTAAT
ACAATGGTTTATGACACAAAAGAAATAGAAAGAATTACAGAAGTTGCAATTAAAATAGCAAATCAAAGAAGTAAAAAAAT
ATGTTCGGTAGATAAATCAAATGTACTAGAAGTAAGTCAATTGTGGAGAGATACAGTTTTAGATGTAGCTTCAAAAAATA
AAGATTTAAGTTTAAGTAATATGTATGTAGATAATGCAGCTATGCAACTTGTAAAAGACCCAAGCCAATTTGACGTTATT
TTAACTAGCAATTTGTTCGGCGATATTTTAAGTGACTTAGCCGCAATGATAACTGGTTCTATTGGAATGCTTCCATCAGC
ATCTTTAAGCAATTCAGGGCCAGGAGTATTTGAACCCGTTCATGGGTCTGCTCCAGACATAGCTGGTAAAAACTTAGCTA
ATCCCATTGCTATGGCTTTATCAACCTCAATGATGCTAAAAATTGGTTTGAATGAGGAAAAAGCTGCAGATGATATTGAA
ATAGCTATTGATAATGTTTTATCAAAAGGATATAGAACATCAGATTTAGACAATGGTGATTGCAACGTTCTATCTTGTAG
TGAAATTGGAGAAAAAATCATTCAAGAAATTTGA

Upstream 100 bases:

>100_bases
TTAAGAAGTTCAAGTATTTTTAAAAAATTGCCTGAATTAGCAAAAAAGCTTCGACAACTAGACAAGAAATAAATTATTCT
CTTAATAAACCAACAATAAA

Downstream 100 bases:

>100_bases
ATAAAAAATTAATAAATTAAAAATTATCTTTAACTAGATCAAAAAGTTGGCATATGACCTGTCAGAATCATTAAATATTG
TTTTAATTAAATGTCTAAAC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MKKYKVVLLSGDGIGPEISEISSEILKKLSHKYSFDIEIKEEYFGGIAYEKYKDPAPKATLDQCKSSDAVLLACVGDLKY
DSLPRELRPESGLLKLRSALNLFANIRPVKIRKSLLDASSLKKEIIEDVDLVVVRELISGIYFGQPRGQITNTKVKKAFN
TMVYDTKEIERITEVAIKIANQRSKKICSVDKSNVLEVSQLWRDTVLDVASKNKDLSLSNMYVDNAAMQLVKDPSQFDVI
LTSNLFGDILSDLAAMITGSIGMLPSASLSNSGPGVFEPVHGSAPDIAGKNLANPIAMALSTSMMLKIGLNEEKAADDIE
IAIDNVLSKGYRTSDLDNGDCNVLSCSEIGEKIIQEI

Sequences:

>Translated_357_residues
MKKYKVVLLSGDGIGPEISEISSEILKKLSHKYSFDIEIKEEYFGGIAYEKYKDPAPKATLDQCKSSDAVLLACVGDLKY
DSLPRELRPESGLLKLRSALNLFANIRPVKIRKSLLDASSLKKEIIEDVDLVVVRELISGIYFGQPRGQITNTKVKKAFN
TMVYDTKEIERITEVAIKIANQRSKKICSVDKSNVLEVSQLWRDTVLDVASKNKDLSLSNMYVDNAAMQLVKDPSQFDVI
LTSNLFGDILSDLAAMITGSIGMLPSASLSNSGPGVFEPVHGSAPDIAGKNLANPIAMALSTSMMLKIGLNEEKAADDIE
IAIDNVLSKGYRTSDLDNGDCNVLSCSEIGEKIIQEI
>Mature_357_residues
MKKYKVVLLSGDGIGPEISEISSEILKKLSHKYSFDIEIKEEYFGGIAYEKYKDPAPKATLDQCKSSDAVLLACVGDLKY
DSLPRELRPESGLLKLRSALNLFANIRPVKIRKSLLDASSLKKEIIEDVDLVVVRELISGIYFGQPRGQITNTKVKKAFN
TMVYDTKEIERITEVAIKIANQRSKKICSVDKSNVLEVSQLWRDTVLDVASKNKDLSLSNMYVDNAAMQLVKDPSQFDVI
LTSNLFGDILSDLAAMITGSIGMLPSASLSNSGPGVFEPVHGSAPDIAGKNLANPIAMALSTSMMLKIGLNEEKAADDIE
IAIDNVLSKGYRTSDLDNGDCNVLSCSEIGEKIIQEI

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=361, Percent_Identity=34.0720221606648, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI28178816, Length=364, Percent_Identity=29.9450549450549, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI28178821, Length=316, Percent_Identity=30.6962025316456, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI4758582, Length=315, Percent_Identity=27.3015873015873, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI28178838, Length=315, Percent_Identity=27.3015873015873, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI28178819, Length=142, Percent_Identity=36.6197183098592, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=47.2067039106145, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1788101, Length=346, Percent_Identity=32.6589595375723, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1787381, Length=309, Percent_Identity=27.1844660194175, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=364, Percent_Identity=29.6703296703297, Blast_Score=138, Evalue=6e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=29.0322580645161, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI25144293, Length=317, Percent_Identity=27.4447949526814, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17505779, Length=314, Percent_Identity=28.6624203821656, Blast_Score=97, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=42.6229508196721, Blast_Score=245, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6322097, Length=358, Percent_Identity=35.4748603351955, Blast_Score=163, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=364, Percent_Identity=30.7692307692308, Blast_Score=130, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=342, Percent_Identity=29.5321637426901, Blast_Score=115, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24643270, Length=361, Percent_Identity=32.9639889196676, Blast_Score=154, Evalue=9e-38,
Organism=Drosophila melanogaster, GI24643268, Length=361, Percent_Identity=32.9639889196676, Blast_Score=154, Evalue=1e-37,
Organism=Drosophila melanogaster, GI24661184, Length=368, Percent_Identity=30.9782608695652, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161078637, Length=364, Percent_Identity=26.6483516483516, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078633, Length=364, Percent_Identity=26.6483516483516, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24650122, Length=364, Percent_Identity=26.6483516483516, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI161078635, Length=364, Percent_Identity=26.6483516483516, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI281362242, Length=343, Percent_Identity=30.6122448979592, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648872, Length=343, Percent_Identity=30.6122448979592, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI161078639, Length=359, Percent_Identity=26.7409470752089, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI20130355, Length=355, Percent_Identity=25.0704225352113, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39072; Mature: 39072

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYKVVLLSGDGIGPEISEISSEILKKLSHKYSFDIEIKEEYFGGIAYEKYKDPAPKAT
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCHHHCCCCCCCHH
LDQCKSSDAVLLACVGDLKYDSLPRELRPESGLLKLRSALNLFANIRPVKIRKSLLDASS
HHHHCCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIIEDVDLVVVRELISGIYFGQPRGQITNTKVKKAFNTMVYDTKEIERITEVAIKIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NQRSKKICSVDKSNVLEVSQLWRDTVLDVASKNKDLSLSNMYVDNAAMQLVKDPSQFDVI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHCCCCHHEEE
LTSNLFGDILSDLAAMITGSIGMLPSASLSNSGPGVFEPVHGSAPDIAGKNLANPIAMAL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
STSMMLKIGLNEEKAADDIEIAIDNVLSKGYRTSDLDNGDCNVLSCSEIGEKIIQEI
HHHHHEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHC
>Mature Secondary Structure
MKKYKVVLLSGDGIGPEISEISSEILKKLSHKYSFDIEIKEEYFGGIAYEKYKDPAPKAT
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHHCCEEEEEEHHHHHCCCCHHHCCCCCCCHH
LDQCKSSDAVLLACVGDLKYDSLPRELRPESGLLKLRSALNLFANIRPVKIRKSLLDASS
HHHHCCCCCEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LKKEIIEDVDLVVVRELISGIYFGQPRGQITNTKVKKAFNTMVYDTKEIERITEVAIKIA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NQRSKKICSVDKSNVLEVSQLWRDTVLDVASKNKDLSLSNMYVDNAAMQLVKDPSQFDVI
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHEEHHHHHHHHHCCCCHHEEE
LTSNLFGDILSDLAAMITGSIGMLPSASLSNSGPGVFEPVHGSAPDIAGKNLANPIAMAL
EEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
STSMMLKIGLNEEKAADDIEIAIDNVLSKGYRTSDLDNGDCNVLSCSEIGEKIIQEI
HHHHHEEECCCHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]