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The map label for this gene is pth

Identifier: 123965518

GI number: 123965518

Start: 260541

End: 261140

Strand: Reverse

Name: pth

Synonym: P9515_02831

Alternate gene names: 123965518

Gene position: 261140-260541 (Counterclockwise)

Preceding gene: 123965519

Following gene: 123965517

Centisome position: 15.32

GC content: 28.83

Gene sequence:

>600_bases
ATGAAGGATAATGAGGTATTTATTATTGGTTTAGGTAATCCTGGTAAACAATATATCAAAAGCAGGCATAATATTGGATT
TTTGTTGTTAGAAACTTTTTCAAAAAAATATGATGCACAATTTACTCTAAAAAATAAGCTGAAAAGCAGATATACAGAAT
TCAAAATAAATGATTCTATCTATAAGTTATTTATGCCTCACACTTATATGAATAATAGTGGAGATGCTGTGAAGGCAATA
GTGGATTGGTACAAGATCAGTTTGGATAAGGTTTTTATAATAGTTGATGATATCGATCTGCCTCTGGGTAAGATTAGATT
CAGAAAAAAAGGAAGTTCTGGAGGACATAATGGCCTGAAAGATATCATAAATAAATTACAGACTGAAAATTTCAATAGAA
TAAAAATAGGTATAGGATCGCCTCCCATTAATGAAACAAAGAAACAACTGAACACGATTTCCCACGTCTTAGGGAATATT
TCTTCTCAAGAGAATTCTACGTTAGAAAAAGTTTATCAAAAAGTAATTGAATCACTAGAAGAATTAGATATTAAAAATGA
AGATTATATTATTAGCGAATTAAATTCTTTCCACAAATAA

Upstream 100 bases:

>100_bases
TATTCAAATCTTATATAAATTGTGTTGTCATACACATTAGAAAAACTTTCACTAATTTCCTATATTTATAATTTAAAAAC
TTTAGATATAAATTGAAATT

Downstream 100 bases:

>100_bases
AAGATTAAATATAATGAAATCTAAACCTGAGACTACAGCAAACGTAAGTGTTAAAGAGTACTGTTTCATTAAAAAAGAAT
TAAAAGGAGTTGTAGAAGCA

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MKDNEVFIIGLGNPGKQYIKSRHNIGFLLLETFSKKYDAQFTLKNKLKSRYTEFKINDSIYKLFMPHTYMNNSGDAVKAI
VDWYKISLDKVFIIVDDIDLPLGKIRFRKKGSSGGHNGLKDIINKLQTENFNRIKIGIGSPPINETKKQLNTISHVLGNI
SSQENSTLEKVYQKVIESLEELDIKNEDYIISELNSFHK

Sequences:

>Translated_199_residues
MKDNEVFIIGLGNPGKQYIKSRHNIGFLLLETFSKKYDAQFTLKNKLKSRYTEFKINDSIYKLFMPHTYMNNSGDAVKAI
VDWYKISLDKVFIIVDDIDLPLGKIRFRKKGSSGGHNGLKDIINKLQTENFNRIKIGIGSPPINETKKQLNTISHVLGNI
SSQENSTLEKVYQKVIESLEELDIKNEDYIISELNSFHK
>Mature_199_residues
MKDNEVFIIGLGNPGKQYIKSRHNIGFLLLETFSKKYDAQFTLKNKLKSRYTEFKINDSIYKLFMPHTYMNNSGDAVKAI
VDWYKISLDKVFIIVDDIDLPLGKIRFRKKGSSGGHNGLKDIINKLQTENFNRIKIGIGSPPINETKKQLNTISHVLGNI
SSQENSTLEKVYQKVIESLEELDIKNEDYIISELNSFHK

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=173, Percent_Identity=26.0115606936416, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1787455, Length=136, Percent_Identity=38.2352941176471, Blast_Score=94, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6321983, Length=171, Percent_Identity=28.6549707602339, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_PROM5 (A2BUN1)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010599.1
- ProteinModelPortal:   A2BUN1
- SMR:   A2BUN1
- STRING:   A2BUN1
- GeneID:   4720154
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_02831
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- ProtClustDB:   PRK05426
- BioCyc:   PMAR167542:P9515ORF_0293-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 22874; Mature: 22874

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNEVFIIGLGNPGKQYIKSRHNIGFLLLETFSKKYDAQFTLKNKLKSRYTEFKINDSI
CCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCHH
YKLFMPHTYMNNSGDAVKAIVDWYKISLDKVFIIVDDIDLPLGKIRFRKKGSSGGHNGLK
HEEECCCCEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCHH
DIINKLQTENFNRIKIGIGSPPINETKKQLNTISHVLGNISSQENSTLEKVYQKVIESLE
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ELDIKNEDYIISELNSFHK
HCCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKDNEVFIIGLGNPGKQYIKSRHNIGFLLLETFSKKYDAQFTLKNKLKSRYTEFKINDSI
CCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHEEECCHH
YKLFMPHTYMNNSGDAVKAIVDWYKISLDKVFIIVDDIDLPLGKIRFRKKGSSGGHNGLK
HEEECCCCEECCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCEEEECCCCCCCCCCHH
DIINKLQTENFNRIKIGIGSPPINETKKQLNTISHVLGNISSQENSTLEKVYQKVIESLE
HHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ELDIKNEDYIISELNSFHK
HCCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA