Click here to switch to the map view.

The map label for this gene is fadD23 [H]

Identifier: 121639744

GI number: 121639744

Start: 4272214

End: 4273968

Strand: Direct

Name: fadD23 [H]

Synonym: BCG_3889

Alternate gene names: 121639744

Gene position: 4272214-4273968 (Clockwise)

Preceding gene: 121639740

Following gene: 121639749

Centisome position: 97.66

GC content: 57.55

Gene sequence:

>1755_bases
ATGGTTTCGCTTTCCATCCCCTCGATGTTGCGCCAGTGCGTCAACCTGCACCCGGACGGCACGGCATTCACTTACATCGA
TTACGAACGGGATTCGGAGGGCATAAGTGAAAGCCTGACGTGGTCGCAGGTGTATCGGCGAACCCTAAACGTTGCAGCAG
AAGTCCGCCGCCATGCCGCAATTGGTGACCGTGCAGTGATATTGGCCCCACAAGGACTCGATTATATTGTTGCTTTTCTG
GGCGCTTTACAGGCCGGTCTTATTGCGGTTCCACTTTCGGCTCCGCTCGGCGGCGCCAGCGATGAACGTGTTGACGCGGT
AGTGCGTGACGCGAAACCCAATGTCGTTCTGACAACATCCGCGATAATGGGCGATGTCGTCCCGCGCGTTACGCCACCGC
CCGGTATTGCCAGCCCGCCAACGGTTGCGGTCGATCAACTAGATCTGGACTCGCCGATACGATCTAATATTGTGGACGAT
TCTCTCCAAACAACCGCATATTTGCAGTATACGTCGGGATCGACCCGCACACCTGCCGGTGTAATGATTACCTACAAGAA
TATATTGGCAAATTTCCAGCAGATGATTTCCGCCTATTTCGCCGACACCGGAGCCGTACCGCCATTGGACCTTTTCATTA
TGTCGTGGCTACCGTTCTATCATGACATGGGTTTGGTTCTGGGAGTTTGTGCGCCGATTATCGTAGGATGCGGCGCTGTG
CTCACAAGCCCGGTGGCGTTTCTGCAGCGACCAGCCCGGTGGCTGCAATTGATGGCACGCGAGGGCCAGGCGTTTTCGGC
GGCACCGAACTTCGCCTTCGAACTGACGGCAGCAAAAGCAATAGATGACGACTTGGCCGGGCTCGACCTTGGACGGATCA
AAACCATCCTCTGCGGCAGTGAAAGGGTGCATCCGGCGACCCTCAAGCGCTTTGTCGACCGGTTTAGCCGTTTCAATCTT
CGAGAATTCGCAATTCGGCCCGCGTACGGACTCGCGGAAGCCACGGTGTATGTGGCGACCAGCCAAGCCGGCCAACCCCC
AGAAATCCGTTACTTCGAACCCCACGAACTTTCCGCTGGGCAGGCCAAGCCGTGCGCAACCGGGGCGGGCACAGCTCTGG
TCAGTTACCCGCTGCCGCAATCACCCATTGTTCGGATCGTCGATCCCAACACCAATACCGAGTGCCCACCCGGAACAATC
GGTGAGATCTGGGTACACGGCGACAATGTCGCCGGCGGCTATTGGGAAAAGCCTGACGAGACTGAACGCACCTTCGGAGG
AGCACTGGTCGCTCCCTCGGCCGGCACACCCGTAGGGCCTTGGCTACGAACTGGCGACTCGGGCTTCGTGTCTGAGGACA
AGTTTTTCATCATCGGCAGAATAAAGGATCTGTTGATTGTTTACGGCCGCAATCATTCTCCCGACGACATCGAGGCAACG
ATCCAGGAGATCACTCGGGGCCGCTGTGCGGCGATAGCGGTTCCGAGCAATGGCGTGGAGAAGCTCGTTGCCATCGTCGA
ACTCAACAACCGCGGCAACTTGGACACAGAGAGGCTGAGCTTCGTCACGCGTGAAGTCACCTCGGCGATATCCACCTCGC
ATGGATTGAGCGTGTCGGATCTGGTTCTGGTGGCGCCCGGCTCGATTCCGATCACCACGAGCGGCAAGGTCAGACGTGCC
GAGTGTGTGAAGCTGTATCGACACAACGAGTTCACCCGGTTGGACGCTAAGCCGTTGCAAGCGAGCGATCTTTAG

Upstream 100 bases:

>100_bases
CGGTTTGTCGTGGCATGGGCAGCCCGCGGTCTGCCGCAGTCTGAAGTTCGCGGCCTGAGCGCGCGCTATCTTCCACGCCG
GGCCGGTAGTCTGACGCTTC

Downstream 100 bases:

>100_bases
TGGTCACGCGACTTGCACCCCGTCTCGGGGTTGTTCGGCAGCCATGCGGCTGCCTCCCTTCCGCGCTTCACAGCCACCAG
CCGGGCAAGGCCCGGTCTTA

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MVSLSIPSMLRQCVNLHPDGTAFTYIDYERDSEGISESLTWSQVYRRTLNVAAEVRRHAAIGDRAVILAPQGLDYIVAFL
GALQAGLIAVPLSAPLGGASDERVDAVVRDAKPNVVLTTSAIMGDVVPRVTPPPGIASPPTVAVDQLDLDSPIRSNIVDD
SLQTTAYLQYTSGSTRTPAGVMITYKNILANFQQMISAYFADTGAVPPLDLFIMSWLPFYHDMGLVLGVCAPIIVGCGAV
LTSPVAFLQRPARWLQLMAREGQAFSAAPNFAFELTAAKAIDDDLAGLDLGRIKTILCGSERVHPATLKRFVDRFSRFNL
REFAIRPAYGLAEATVYVATSQAGQPPEIRYFEPHELSAGQAKPCATGAGTALVSYPLPQSPIVRIVDPNTNTECPPGTI
GEIWVHGDNVAGGYWEKPDETERTFGGALVAPSAGTPVGPWLRTGDSGFVSEDKFFIIGRIKDLLIVYGRNHSPDDIEAT
IQEITRGRCAAIAVPSNGVEKLVAIVELNNRGNLDTERLSFVTREVTSAISTSHGLSVSDLVLVAPGSIPITTSGKVRRA
ECVKLYRHNEFTRLDAKPLQASDL

Sequences:

>Translated_584_residues
MVSLSIPSMLRQCVNLHPDGTAFTYIDYERDSEGISESLTWSQVYRRTLNVAAEVRRHAAIGDRAVILAPQGLDYIVAFL
GALQAGLIAVPLSAPLGGASDERVDAVVRDAKPNVVLTTSAIMGDVVPRVTPPPGIASPPTVAVDQLDLDSPIRSNIVDD
SLQTTAYLQYTSGSTRTPAGVMITYKNILANFQQMISAYFADTGAVPPLDLFIMSWLPFYHDMGLVLGVCAPIIVGCGAV
LTSPVAFLQRPARWLQLMAREGQAFSAAPNFAFELTAAKAIDDDLAGLDLGRIKTILCGSERVHPATLKRFVDRFSRFNL
REFAIRPAYGLAEATVYVATSQAGQPPEIRYFEPHELSAGQAKPCATGAGTALVSYPLPQSPIVRIVDPNTNTECPPGTI
GEIWVHGDNVAGGYWEKPDETERTFGGALVAPSAGTPVGPWLRTGDSGFVSEDKFFIIGRIKDLLIVYGRNHSPDDIEAT
IQEITRGRCAAIAVPSNGVEKLVAIVELNNRGNLDTERLSFVTREVTSAISTSHGLSVSDLVLVAPGSIPITTSGKVRRA
ECVKLYRHNEFTRLDAKPLQASDL
>Mature_584_residues
MVSLSIPSMLRQCVNLHPDGTAFTYIDYERDSEGISESLTWSQVYRRTLNVAAEVRRHAAIGDRAVILAPQGLDYIVAFL
GALQAGLIAVPLSAPLGGASDERVDAVVRDAKPNVVLTTSAIMGDVVPRVTPPPGIASPPTVAVDQLDLDSPIRSNIVDD
SLQTTAYLQYTSGSTRTPAGVMITYKNILANFQQMISAYFADTGAVPPLDLFIMSWLPFYHDMGLVLGVCAPIIVGCGAV
LTSPVAFLQRPARWLQLMAREGQAFSAAPNFAFELTAAKAIDDDLAGLDLGRIKTILCGSERVHPATLKRFVDRFSRFNL
REFAIRPAYGLAEATVYVATSQAGQPPEIRYFEPHELSAGQAKPCATGAGTALVSYPLPQSPIVRIVDPNTNTECPPGTI
GEIWVHGDNVAGGYWEKPDETERTFGGALVAPSAGTPVGPWLRTGDSGFVSEDKFFIIGRIKDLLIVYGRNHSPDDIEAT
IQEITRGRCAAIAVPSNGVEKLVAIVELNNRGNLDTERLSFVTREVTSAISTSHGLSVSDLVLVAPGSIPITTSGKVRRA
ECVKLYRHNEFTRLDAKPLQASDL

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=573, Percent_Identity=22.6876090750436, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI55749758, Length=578, Percent_Identity=24.5674740484429, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI225735629, Length=559, Percent_Identity=24.1502683363148, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI45827692, Length=559, Percent_Identity=24.1502683363148, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI225735625, Length=566, Percent_Identity=22.9681978798587, Blast_Score=86, Evalue=8e-17,
Organism=Escherichia coli, GI1788107, Length=595, Percent_Identity=26.7226890756303, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI193202679, Length=562, Percent_Identity=24.0213523131673, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=568, Percent_Identity=24.4718309859155, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531443, Length=484, Percent_Identity=22.5206611570248, Blast_Score=82, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=530, Percent_Identity=22.8301886792453, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24654656, Length=469, Percent_Identity=23.8805970149254, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 62841; Mature: 62841

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSLSIPSMLRQCVNLHPDGTAFTYIDYERDSEGISESLTWSQVYRRTLNVAAEVRRHAA
CCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHC
IGDRAVILAPQGLDYIVAFLGALQAGLIAVPLSAPLGGASDERVDAVVRDAKPNVVLTTS
CCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEH
AIMGDVVPRVTPPPGIASPPTVAVDQLDLDSPIRSNIVDDSLQTTAYLQYTSGSTRTPAG
HHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCE
VMITYKNILANFQQMISAYFADTGAVPPLDLFIMSWLPFYHDMGLVLGVCAPIIVGCGAV
EEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LTSPVAFLQRPARWLQLMAREGQAFSAAPNFAFELTAAKAIDDDLAGLDLGRIKTILCGS
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEHHHHHCCHHCCCCHHHHHEEEECC
ERVHPATLKRFVDRFSRFNLREFAIRPAYGLAEATVYVATSQAGQPPEIRYFEPHELSAG
CCCCHHHHHHHHHHHHHCCHHHHEECCCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCC
QAKPCATGAGTALVSYPLPQSPIVRIVDPNTNTECPPGTIGEIWVHGDNVAGGYWEKPDE
CCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCH
TERTFGGALVAPSAGTPVGPWLRTGDSGFVSEDKFFIIGRIKDLLIVYGRNHSPDDIEAT
HHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEEECCCCCCHHHHHH
IQEITRGRCAAIAVPSNGVEKLVAIVELNNRGNLDTERLSFVTREVTSAISTSHGLSVSD
HHHHCCCCEEEEEECCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
LVLVAPGSIPITTSGKVRRAECVKLYRHNEFTRLDAKPLQASDL
EEEECCCCEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MVSLSIPSMLRQCVNLHPDGTAFTYIDYERDSEGISESLTWSQVYRRTLNVAAEVRRHAA
CCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHC
IGDRAVILAPQGLDYIVAFLGALQAGLIAVPLSAPLGGASDERVDAVVRDAKPNVVLTTS
CCCCEEEECCCCHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEH
AIMGDVVPRVTPPPGIASPPTVAVDQLDLDSPIRSNIVDDSLQTTAYLQYTSGSTRTPAG
HHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHCCCCCCCEEEEEECCCCCCCCCE
VMITYKNILANFQQMISAYFADTGAVPPLDLFIMSWLPFYHDMGLVLGVCAPIIVGCGAV
EEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LTSPVAFLQRPARWLQLMAREGQAFSAAPNFAFELTAAKAIDDDLAGLDLGRIKTILCGS
HHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEHHHHHCCHHCCCCHHHHHEEEECC
ERVHPATLKRFVDRFSRFNLREFAIRPAYGLAEATVYVATSQAGQPPEIRYFEPHELSAG
CCCCHHHHHHHHHHHHHCCHHHHEECCCCCCCEEEEEEEECCCCCCCCCEEECCCCCCCC
QAKPCATGAGTALVSYPLPQSPIVRIVDPNTNTECPPGTIGEIWVHGDNVAGGYWEKPDE
CCCCCCCCCCCEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCH
TERTFGGALVAPSAGTPVGPWLRTGDSGFVSEDKFFIIGRIKDLLIVYGRNHSPDDIEAT
HHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECEEEEEECCCCCCHHHHHH
IQEITRGRCAAIAVPSNGVEKLVAIVELNNRGNLDTERLSFVTREVTSAISTSHGLSVSD
HHHHCCCCEEEEEECCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEE
LVLVAPGSIPITTSGKVRRAECVKLYRHNEFTRLDAKPLQASDL
EEEECCCCEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972; 1527058 [H]