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The map label for this gene is ponA2 [H]

Identifier: 121639596

GI number: 121639596

Start: 4095029

End: 4097461

Strand: Direct

Name: ponA2 [H]

Synonym: BCG_3741

Alternate gene names: 121639596

Gene position: 4095029-4097461 (Clockwise)

Preceding gene: 121639594

Following gene: 121639597

Centisome position: 93.61

GC content: 65.31

Gene sequence:

>2433_bases
ATGCCCGAGCGCCTCCCGGCCGCGATCACCGTTCTGAAGCTGGCTGGGTGCTGTCTGTTGGCCAGTGTCGTCGCCACTGC
GCTGACGTTCCCGTTCGCAGGCGGGCTAGGGCTGATGTCCAATCGTGCCTCTGAGGTCGTTGCCAACGGCTCGGCCCAGC
TGCTCGAGGGGCAAGTGCCTGCGGTATCGACGATGGTCGACGCGAAGGGCAACACGATCGCGTGGCTGTACTCGCAGCGC
CGGTTCGAGGTGCCCTCGGACAAGATCGCCAACACGATGAAGCTGGCGATCGTCTCGATTGAAGATAAGCGGTTCGCCGA
CCACAGCGGCGTGGACTGGAAGGGCACCCTGACCGGCCTGGCGGGCTACGCGTCCGGCGACCTCGACACGCGCGGCGGCT
CGACGCTCGAACAACAGTACGTGAAGAACTACCAACTGCTGGTGACAGCCCAAACCGATGCCGAGAAGCGAGCGGCCGTC
GAAACCACTCCGGCCCGCAAGCTTCGCGAGATCCGGATGGCACTCACGCTGGACAAGACCTTCACAAAATCTGAAATCCT
GACCCGATACTTGAACCTGGTCTCGTTCGGCAATAACTCGTTCGGCGTGCAGGACGCGGCGCAAACGTACTTCGGCATCA
ACGCGTCCGACCTGAATTGGCAGCAAGCGGCGCTGCTGGCCGGCATGGTGCAATCGACCAGCACGCTCAACCCGTACACC
AACCCCGACGGCGCGCTGGCCCGGCGGAACGTGGTCCTCGACACCATGATCGAGAACCTTCCCGGGGAGGCGGAGGCGTT
GCGTGCCGCCAAGGCCGAGCCGCTGGGGGTACTGCCGCAGCCCAATGAGTTGCCGCGCGGCTGCATCGCGGCCGGCGACC
GCGCATTCTTCTGCGACTACGTCCAGGAGTACCTGTCTCGGGCCGGGATCAGCAAGGAGCAGGTCGCCACGGGCGGGTAC
CTGATCCGCACCACCCTGGACCCAGAGGTGCAGGCACCGGTCAAGGCCGCCATCGACAAGTACGCCAGCCCGAACCTGGC
CGGTATTTCCAGCGTGATGAGCGTGATCAAACCGGGTAAGGATGCGCACAAGGTGTTGGCCATGGCCAGTAACCGCAAAT
ACGGGCTGGATCTAGAAGCCGGCGAAACCATGCGGCCGCAGCCATTCTCCCTGGTTGGCGACGGCGCCGGGTCTATCTTC
AAGATCTTCACCACGGCCGCTGCTCTGGACATGGGCATGGGTATTAACGCCCAACTCGACGTGCCGCCCCGATTCCAGGC
CAAAGGTCTGGGAAGTGGCGGGGCAAAGGGGTGCCCCAAAGAGACCTGGTGTGTGGTGAACGCCGGCAACTACCGCGGCT
CGATGAATGTCACCGACGCGCTGGCAACCTCGCCAAACACCGCGTTCGCCAAGCTGATCTCGCAGGTCGGGGTGGGGCGT
GCGGTCGATATGGCCATCAAACTCGGGCTGAGGTCTTATGCGAATCCCGGCACCGCACGCGACTACAACCCCGACAGCAA
TGAGAGCTTGGCTGACTTCGTCAAACGACAGAACCTGGGTTCGTTCACCCTCGGCCCCATCGAGTTAAACGCGCTGGAGC
TGTCCAACGTGGCGGCCACGTTGGCATCCGGCGGCGTGTGGTGCCCCCCCAACCCAATCGACCAGCTCATCGACCGCAAC
GGCAACGAAGTCGCGGTCACCACCGAGACGTGCGACCAGGTGGTGCCCGCAGGGCTGGCGAACACCCTCGCCAACGCGAT
GAGCAAGGACGCCGTGGGCAGCGGCACGGCGGCCGGTTCGGCCGGCGCGGCGGGCTGGGATCTGCCGATGTCCGGCAAAA
CCGGCACCACCGAGGCGCACCGGTCGGCCGGCTTCGTGGGCTTCACCAACCGCTACGCGGCGGCGAACTACATCTACGAC
GACTCCAGCTCGCCGACAGATCTGTGTTCCGGCCCGCTGCGCCATTGCGGCAGCGGCGACTTGTACGGCGGCAACGAGCC
ATCCCGCACCTGGTTCGCCGCGATGAAGCCGATCGCCAACAACTTCGGCGAAGTGCAGCTACCACCGACCGATCCACGCT
ATGTCGACGGCGCACCAGGCTCACGGGTACCAAGCGTGGCCGGTCTGGATGTCGACGCCGCACGCCAGCGCCTCAAGGAC
GCGGGCTTCCAGGTCGCCGACCAAACCAACTCGGTCAACAGCTCCGCCAAGTATGGTGAGGTGGTCGGAACGTCGCCCAG
CGGTCAAACAATTCCGGGTTCGATCGTCACGATCCAGATCAGCAACGGCATCCCGCCGGCTCCGCCTCCGCCACCGCTGC
CTGAGGATGGTGGGCCGCCACCGCCGGTCGGATCGCAGGTGGTGGAGATTCCGGGGCTGCCGCCGATCACCATTCCGCTG
CTGGCGCCACCACCCCCAGCGCCTCCCCCGTAG

Upstream 100 bases:

>100_bases
CGCGCCCGCCCCGATCCCGCAGTGACTCACCCGCCCGCGGAAAGATTCTATTGGACCGAGCGGCACGGTGGAGTGACAGG
AGGTCGCTACTGTAGTACGC

Downstream 100 bases:

>100_bases
GCCCTCCCAATCGGCCTCGTGCCGCTGCAGACGCGCGATCAGACCTCGACCGGCAGTAGGCTGCGTGCATGGCTGCTGTC
TTGCCCACCTTGATCCGCAC

Product: putative bifunctional membrane-associated penicillin-binding protein 1A/1B ponA2

Products: NA

Alternate protein names: PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 810; Mature: 809

Protein sequence:

>810_residues
MPERLPAAITVLKLAGCCLLASVVATALTFPFAGGLGLMSNRASEVVANGSAQLLEGQVPAVSTMVDAKGNTIAWLYSQR
RFEVPSDKIANTMKLAIVSIEDKRFADHSGVDWKGTLTGLAGYASGDLDTRGGSTLEQQYVKNYQLLVTAQTDAEKRAAV
ETTPARKLREIRMALTLDKTFTKSEILTRYLNLVSFGNNSFGVQDAAQTYFGINASDLNWQQAALLAGMVQSTSTLNPYT
NPDGALARRNVVLDTMIENLPGEAEALRAAKAEPLGVLPQPNELPRGCIAAGDRAFFCDYVQEYLSRAGISKEQVATGGY
LIRTTLDPEVQAPVKAAIDKYASPNLAGISSVMSVIKPGKDAHKVLAMASNRKYGLDLEAGETMRPQPFSLVGDGAGSIF
KIFTTAAALDMGMGINAQLDVPPRFQAKGLGSGGAKGCPKETWCVVNAGNYRGSMNVTDALATSPNTAFAKLISQVGVGR
AVDMAIKLGLRSYANPGTARDYNPDSNESLADFVKRQNLGSFTLGPIELNALELSNVAATLASGGVWCPPNPIDQLIDRN
GNEVAVTTETCDQVVPAGLANTLANAMSKDAVGSGTAAGSAGAAGWDLPMSGKTGTTEAHRSAGFVGFTNRYAAANYIYD
DSSSPTDLCSGPLRHCGSGDLYGGNEPSRTWFAAMKPIANNFGEVQLPPTDPRYVDGAPGSRVPSVAGLDVDAARQRLKD
AGFQVADQTNSVNSSAKYGEVVGTSPSGQTIPGSIVTIQISNGIPPAPPPPPLPEDGGPPPPVGSQVVEIPGLPPITIPL
LAPPPPAPPP

Sequences:

>Translated_810_residues
MPERLPAAITVLKLAGCCLLASVVATALTFPFAGGLGLMSNRASEVVANGSAQLLEGQVPAVSTMVDAKGNTIAWLYSQR
RFEVPSDKIANTMKLAIVSIEDKRFADHSGVDWKGTLTGLAGYASGDLDTRGGSTLEQQYVKNYQLLVTAQTDAEKRAAV
ETTPARKLREIRMALTLDKTFTKSEILTRYLNLVSFGNNSFGVQDAAQTYFGINASDLNWQQAALLAGMVQSTSTLNPYT
NPDGALARRNVVLDTMIENLPGEAEALRAAKAEPLGVLPQPNELPRGCIAAGDRAFFCDYVQEYLSRAGISKEQVATGGY
LIRTTLDPEVQAPVKAAIDKYASPNLAGISSVMSVIKPGKDAHKVLAMASNRKYGLDLEAGETMRPQPFSLVGDGAGSIF
KIFTTAAALDMGMGINAQLDVPPRFQAKGLGSGGAKGCPKETWCVVNAGNYRGSMNVTDALATSPNTAFAKLISQVGVGR
AVDMAIKLGLRSYANPGTARDYNPDSNESLADFVKRQNLGSFTLGPIELNALELSNVAATLASGGVWCPPNPIDQLIDRN
GNEVAVTTETCDQVVPAGLANTLANAMSKDAVGSGTAAGSAGAAGWDLPMSGKTGTTEAHRSAGFVGFTNRYAAANYIYD
DSSSPTDLCSGPLRHCGSGDLYGGNEPSRTWFAAMKPIANNFGEVQLPPTDPRYVDGAPGSRVPSVAGLDVDAARQRLKD
AGFQVADQTNSVNSSAKYGEVVGTSPSGQTIPGSIVTIQISNGIPPAPPPPPLPEDGGPPPPVGSQVVEIPGLPPITIPL
LAPPPPAPPP
>Mature_809_residues
PERLPAAITVLKLAGCCLLASVVATALTFPFAGGLGLMSNRASEVVANGSAQLLEGQVPAVSTMVDAKGNTIAWLYSQRR
FEVPSDKIANTMKLAIVSIEDKRFADHSGVDWKGTLTGLAGYASGDLDTRGGSTLEQQYVKNYQLLVTAQTDAEKRAAVE
TTPARKLREIRMALTLDKTFTKSEILTRYLNLVSFGNNSFGVQDAAQTYFGINASDLNWQQAALLAGMVQSTSTLNPYTN
PDGALARRNVVLDTMIENLPGEAEALRAAKAEPLGVLPQPNELPRGCIAAGDRAFFCDYVQEYLSRAGISKEQVATGGYL
IRTTLDPEVQAPVKAAIDKYASPNLAGISSVMSVIKPGKDAHKVLAMASNRKYGLDLEAGETMRPQPFSLVGDGAGSIFK
IFTTAAALDMGMGINAQLDVPPRFQAKGLGSGGAKGCPKETWCVVNAGNYRGSMNVTDALATSPNTAFAKLISQVGVGRA
VDMAIKLGLRSYANPGTARDYNPDSNESLADFVKRQNLGSFTLGPIELNALELSNVAATLASGGVWCPPNPIDQLIDRNG
NEVAVTTETCDQVVPAGLANTLANAMSKDAVGSGTAAGSAGAAGWDLPMSGKTGTTEAHRSAGFVGFTNRYAAANYIYDD
SSSPTDLCSGPLRHCGSGDLYGGNEPSRTWFAAMKPIANNFGEVQLPPTDPRYVDGAPGSRVPSVAGLDVDAARQRLKDA
GFQVADQTNSVNSSAKYGEVVGTSPSGQTIPGSIVTIQISNGIPPAPPPPPLPEDGGPPPPVGSQVVEIPGLPPITIPLL
APPPPAPPP

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass type II membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=307, Percent_Identity=30.2931596091205, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1786343, Length=590, Percent_Identity=25.5932203389831, Blast_Score=110, Evalue=4e-25,
Organism=Escherichia coli, GI1789601, Length=210, Percent_Identity=32.3809523809524, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788867, Length=452, Percent_Identity=24.5575221238938, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 84636; Mature: 84505

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERLPAAITVLKLAGCCLLASVVATALTFPFAGGLGLMSNRASEVVANGSAQLLEGQVP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCCHHHHCCCCC
AVSTMVDAKGNTIAWLYSQRRFEVPSDKIANTMKLAIVSIEDKRFADHSGVDWKGTLTGL
HHHHEECCCCCEEEEEEECCCCCCCHHHHHCEEEEEEEEECCCCCCCCCCCCCCEEHHHH
AGYASGDLDTRGGSTLEQQYVKNYQLLVTAQTDAEKRAAVETTPARKLREIRMALTLDKT
CCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCH
FTKSEILTRYLNLVSFGNNSFGVQDAAQTYFGINASDLNWQQAALLAGMVQSTSTLNPYT
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
NPDGALARRNVVLDTMIENLPGEAEALRAAKAEPLGVLPQPNELPRGCIAAGDRAFFCDY
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHHHH
VQEYLSRAGISKEQVATGGYLIRTTLDPEVQAPVKAAIDKYASPNLAGISSVMSVIKPGK
HHHHHHHCCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DAHKVLAMASNRKYGLDLEAGETMRPQPFSLVGDGAGSIFKIFTTAAALDMGMGINAQLD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEC
VPPRFQAKGLGSGGAKGCPKETWCVVNAGNYRGSMNVTDALATSPNTAFAKLISQVGVGR
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCH
AVDMAIKLGLRSYANPGTARDYNPDSNESLADFVKRQNLGSFTLGPIELNALELSNVAAT
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEEEEEEHHHHHHH
LASGGVWCPPNPIDQLIDRNGNEVAVTTETCDQVVPAGLANTLANAMSKDAVGSGTAAGS
HHCCCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AGAAGWDLPMSGKTGTTEAHRSAGFVGFTNRYAAANYIYDDSSSPTDLCSGPLRHCGSGD
CCCCCCCCCCCCCCCCCHHHHCCCCEECCCCEEEEEEEECCCCCCHHHHCCHHHHCCCCC
LYGGNEPSRTWFAAMKPIANNFGEVQLPPTDPRYVDGAPGSRVPSVAGLDVDAARQRLKD
CCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
AGFQVADQTNSVNSSAKYGEVVGTSPSGQTIPGSIVTIQISNGIPPAPPPPPLPEDGGPP
CCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PPVGSQVVEIPGLPPITIPLLAPPPPAPPP
CCCCCCEEECCCCCCEEEEEECCCCCCCCC
>Mature Secondary Structure 
PERLPAAITVLKLAGCCLLASVVATALTFPFAGGLGLMSNRASEVVANGSAQLLEGQVP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHCCCHHHHCCCCC
AVSTMVDAKGNTIAWLYSQRRFEVPSDKIANTMKLAIVSIEDKRFADHSGVDWKGTLTGL
HHHHEECCCCCEEEEEEECCCCCCCHHHHHCEEEEEEEEECCCCCCCCCCCCCCEEHHHH
AGYASGDLDTRGGSTLEQQYVKNYQLLVTAQTDAEKRAAVETTPARKLREIRMALTLDKT
CCCCCCCCCCCCCCHHHHHHHCCEEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHCCH
FTKSEILTRYLNLVSFGNNSFGVQDAAQTYFGINASDLNWQQAALLAGMVQSTSTLNPYT
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
NPDGALARRNVVLDTMIENLPGEAEALRAAKAEPLGVLPQPNELPRGCIAAGDRAFFCDY
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCHHHHHH
VQEYLSRAGISKEQVATGGYLIRTTLDPEVQAPVKAAIDKYASPNLAGISSVMSVIKPGK
HHHHHHHCCCCHHHCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCC
DAHKVLAMASNRKYGLDLEAGETMRPQPFSLVGDGAGSIFKIFTTAAALDMGMGINAQLD
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCEEEC
VPPRFQAKGLGSGGAKGCPKETWCVVNAGNYRGSMNVTDALATSPNTAFAKLISQVGVGR
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHCCCH
AVDMAIKLGLRSYANPGTARDYNPDSNESLADFVKRQNLGSFTLGPIELNALELSNVAAT
HHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCEEEEEEEHHHHHHH
LASGGVWCPPNPIDQLIDRNGNEVAVTTETCDQVVPAGLANTLANAMSKDAVGSGTAAGS
HHCCCCCCCCCHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
AGAAGWDLPMSGKTGTTEAHRSAGFVGFTNRYAAANYIYDDSSSPTDLCSGPLRHCGSGD
CCCCCCCCCCCCCCCCCHHHHCCCCEECCCCEEEEEEEECCCCCCHHHHCCHHHHCCCCC
LYGGNEPSRTWFAAMKPIANNFGEVQLPPTDPRYVDGAPGSRVPSVAGLDVDAARQRLKD
CCCCCCCCHHHHHHHHHHHCCCCEEECCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHH
AGFQVADQTNSVNSSAKYGEVVGTSPSGQTIPGSIVTIQISNGIPPAPPPPPLPEDGGPP
CCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
PPVGSQVVEIPGLPPITIPLLAPPPPAPPP
CCCCCCEEECCCCCCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA