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The map label for this gene is dacB1 [H]

Identifier: 121639258

GI number: 121639258

Start: 3707075

End: 3708292

Strand: Direct

Name: dacB1 [H]

Synonym: BCG_3400

Alternate gene names: 121639258

Gene position: 3707075-3708292 (Clockwise)

Preceding gene: 121639257

Following gene: 121639259

Centisome position: 84.74

GC content: 70.28

Gene sequence:

>1218_bases
ATGGCCTTCTTACGTTCGGTATCGTGCCTGGCAGCAGCCGTGTTTGCGGTAGGCACCGGAATTGGTCTACCTACCGCGGC
CGGCGAACCCAATGCCGCACCGGCGGCGTGCCCGTACAAGGTGTCCACCCCACCCGCCGTGGACTCGTCGGAGGTTCCCG
CGGCCGGTGAACCCCCACTGCCGCTGGTGGTACCCCCCACCCCGGTCGGCGGCAACGCGCTGGGCGGCTGCGGCATCATC
ACCGCCCCTGGCAGCGCGCCAGCGCCCGGCGACGTCTCAGCCGAGGCCTGGCTGGTGGCGGACCTGGACAGCGGCGCGGT
GATCGCCGCCCGGGATCCGCACGGCCGGCACCGCCCGGCCAGCGTCATCAAGGTGCTGGTGGCGATGGCGTCCATCAACA
CGCTCACCCTCAACAAGTCGGTCGCCGGAACCGCCGACGACGCGGCGGTCGAGGGCACCAAAGTCGGGGTGAACACCGGT
GGCACCTACACCGTCAACCAGCTGCTGCACGGGCTGCTGATGCACTCCGGCAACGACGCTGCGTACGCGCTGGCCAGGCA
GCTCGGCGGCATGCCGGCCGCGCTGGAGAAAATCAATCTGCTGGCCGCCAAGCTGGGCGGCCGGGACACCCGAGTGGCCA
CGCCGTCCGGACTGGACGGGCCCGGCATGAGCACGTCGGCCTATGACATCGGCCTGTTCTACCGGTACGCGTGGCAGAAC
CCGGTCTTCGCCGACATCGTCGCGACCCGCACCTTCGACTTCCCGGGGCACGGCGACCATCCAGGCTACGAGTTGGAGAA
CGACAACCAGCTGCTCTACAACTATCCGGGCGCGCTCGGCGGCAAGACCGGCTATACCGACGACGCGGGGCAGACCTTCG
TGGGCGCGGCCAACCGCGACGGCCGGCGGCTGATGACGGTGCTGCTGCACGGGACCCGGCAGCCGATCCCGCCGTGGGAG
CAGGCGGCGCACCTGCTCGACTACGGGTTCAACACCCCGGCAGGCACCCAGATCGGGACACTGATCGAACCCGACCCGTC
GCTGATGTCCACCGACCGCAATCCCGCCGACCGGCAACGAGTCGACCCCCAGGCCGCGGCGCGGATATCGGCCGCCGACG
CCCTTCCGGTGCGGGTTGGCGTGGCCGTCATCGGCGCCCTGATCGTGTTCGGGTTGATCATGGTCGCGCGGGCGATGAAC
CGCCGGCCGCAGCACTAG

Upstream 100 bases:

>100_bases
CCGATACCGGACGCAAATATCTTCATCTGTAACTTTCGTCCCGCCAGTCACAGCGCGGCTCCTCGCGGTCGGGCCGCCGA
TCACCTACTCTGCACAGACG

Downstream 100 bases:

>100_bases
CTGCTTACCCCGATACCTTCGGCGTCGTTTGCGGGCGGGCATCCTAGCCGGCCTTGGTCGGCACCGAAATCGGGGCTTGA
CCAGCGGTTGACCGCGTGAC

Product: putative penicillin-binding protein dacB1

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 5*; PBP-5* [H]

Number of amino acids: Translated: 405; Mature: 404

Protein sequence:

>405_residues
MAFLRSVSCLAAAVFAVGTGIGLPTAAGEPNAAPAACPYKVSTPPAVDSSEVPAAGEPPLPLVVPPTPVGGNALGGCGII
TAPGSAPAPGDVSAEAWLVADLDSGAVIAARDPHGRHRPASVIKVLVAMASINTLTLNKSVAGTADDAAVEGTKVGVNTG
GTYTVNQLLHGLLMHSGNDAAYALARQLGGMPAALEKINLLAAKLGGRDTRVATPSGLDGPGMSTSAYDIGLFYRYAWQN
PVFADIVATRTFDFPGHGDHPGYELENDNQLLYNYPGALGGKTGYTDDAGQTFVGAANRDGRRLMTVLLHGTRQPIPPWE
QAAHLLDYGFNTPAGTQIGTLIEPDPSLMSTDRNPADRQRVDPQAAARISAADALPVRVGVAVIGALIVFGLIMVARAMN
RRPQH

Sequences:

>Translated_405_residues
MAFLRSVSCLAAAVFAVGTGIGLPTAAGEPNAAPAACPYKVSTPPAVDSSEVPAAGEPPLPLVVPPTPVGGNALGGCGII
TAPGSAPAPGDVSAEAWLVADLDSGAVIAARDPHGRHRPASVIKVLVAMASINTLTLNKSVAGTADDAAVEGTKVGVNTG
GTYTVNQLLHGLLMHSGNDAAYALARQLGGMPAALEKINLLAAKLGGRDTRVATPSGLDGPGMSTSAYDIGLFYRYAWQN
PVFADIVATRTFDFPGHGDHPGYELENDNQLLYNYPGALGGKTGYTDDAGQTFVGAANRDGRRLMTVLLHGTRQPIPPWE
QAAHLLDYGFNTPAGTQIGTLIEPDPSLMSTDRNPADRQRVDPQAAARISAADALPVRVGVAVIGALIVFGLIMVARAMN
RRPQH
>Mature_404_residues
AFLRSVSCLAAAVFAVGTGIGLPTAAGEPNAAPAACPYKVSTPPAVDSSEVPAAGEPPLPLVVPPTPVGGNALGGCGIIT
APGSAPAPGDVSAEAWLVADLDSGAVIAARDPHGRHRPASVIKVLVAMASINTLTLNKSVAGTADDAAVEGTKVGVNTGG
TYTVNQLLHGLLMHSGNDAAYALARQLGGMPAALEKINLLAAKLGGRDTRVATPSGLDGPGMSTSAYDIGLFYRYAWQNP
VFADIVATRTFDFPGHGDHPGYELENDNQLLYNYPGALGGKTGYTDDAGQTFVGAANRDGRRLMTVLLHGTRQPIPPWEQ
AAHLLDYGFNTPAGTQIGTLIEPDPSLMSTDRNPADRQRVDPQAAARISAADALPVRVGVAVIGALIVFGLIMVARAMNR
RPQH

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors. Required specifically for the synthesis of the spore form of peptidoglycan (cortex) [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Forespore outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=267, Percent_Identity=28.4644194756554, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787062, Length=295, Percent_Identity=27.4576271186441, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1786851, Length=258, Percent_Identity=27.1317829457364, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 41683; Mature: 41552

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFLRSVSCLAAAVFAVGTGIGLPTAAGEPNAAPAACPYKVSTPPAVDSSEVPAAGEPPL
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
PLVVPPTPVGGNALGGCGIITAPGSAPAPGDVSAEAWLVADLDSGAVIAARDPHGRHRPA
CEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCHH
SVIKVLVAMASINTLTLNKSVAGTADDAAVEGTKVGVNTGGTYTVNQLLHGLLMHSGNDA
HHHHHHHHHHHCCEEEECCCCCCCCCCHHCCCCEEEECCCCCEEHHHHHHHHHHCCCCHH
AYALARQLGGMPAALEKINLLAAKLGGRDTRVATPSGLDGPGMSTSAYDIGLFYRYAWQN
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCC
PVFADIVATRTFDFPGHGDHPGYELENDNQLLYNYPGALGGKTGYTDDAGQTFVGAANRD
CHHHHHHHHEEECCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCC
GRRLMTVLLHGTRQPIPPWEQAAHLLDYGFNTPAGTQIGTLIEPDPSLMSTDRNPADRQR
CHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHCCCCCCHHHHH
VDPQAAARISAADALPVRVGVAVIGALIVFGLIMVARAMNRRPQH
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
AFLRSVSCLAAAVFAVGTGIGLPTAAGEPNAAPAACPYKVSTPPAVDSSEVPAAGEPPL
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
PLVVPPTPVGGNALGGCGIITAPGSAPAPGDVSAEAWLVADLDSGAVIAARDPHGRHRPA
CEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEECCCCCCCCHH
SVIKVLVAMASINTLTLNKSVAGTADDAAVEGTKVGVNTGGTYTVNQLLHGLLMHSGNDA
HHHHHHHHHHHCCEEEECCCCCCCCCCHHCCCCEEEECCCCCEEHHHHHHHHHHCCCCHH
AYALARQLGGMPAALEKINLLAAKLGGRDTRVATPSGLDGPGMSTSAYDIGLFYRYAWQN
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEEEEEEEECCCC
PVFADIVATRTFDFPGHGDHPGYELENDNQLLYNYPGALGGKTGYTDDAGQTFVGAANRD
CHHHHHHHHEEECCCCCCCCCCCEECCCCEEEEECCCCCCCCCCCCCCCCCEEEECCCCC
GRRLMTVLLHGTRQPIPPWEQAAHLLDYGFNTPAGTQIGTLIEPDPSLMSTDRNPADRQR
CHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHCCCCCCHHHHH
VDPQAAARISAADALPVRVGVAVIGALIVFGLIMVARAMNRRPQH
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1548223; 7934829; 9384377 [H]